Abstract:
:Using (13)C-labeled glucose fed to a penicillin-overproducing strain of Penicillium chrysogenum, the intracellular fluxes were quantified, and the presence of two new pathways, not previously described in this organism, is suggested. Thus, glycine was synthesized not only by serine hydroxymethyltransferase, but also by threonine aldolase. The formation of cytosolic acetyl-CoA was found to be synthesized both via the citrate lyase-catalyzed reaction and by degradation of the penicillin side-chain precursor, phenoxyacetic acid. Furthermore, the experimental data indicate that the main activities of homocitrate synthase and alpha-isopropylmalate synthase are located in the cytosol. All experimental data on the labeling patterns were obtained using gas chromatography-mass spectrometry, which is faster and more sensitive than the nuclear magnetic resonance methods usually applied for analysis of labeling patterns.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Christensen B,Nielsen Jsubject
Has Abstractpub_date
2000-06-20 00:00:00pages
652-9issue
6eissn
0006-3592issn
1097-0290pii
10.1002/(SICI)1097-0290(20000620)68:6<652::AID-BITjournal_volume
68pub_type
杂志文章abstract::The surface hydrophobicity of a protein is an important factor for its interactions in solution and thus the outcome of its production process. Yet most of the methods are not able to evaluate the influence of these hydrophobic interactions under natural conditions. In the present work we have established a high resol...
journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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abstract::A quick technique for determination of kinetic parameters of fermentation processes is proposed and applied to the transformation of D-xylose into ethanol by Pichia stipitis. The commonly used method to evaluate these parameters is based on achieving several steady states. In the proposed procedure, micro(m) and K(s) ...
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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更新日期:2007-03-01 00:00:00
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更新日期:2003-10-05 00:00:00