On the estimation of average shear rate in bubble columns.

abstract：:The effect of protein concentration in partitioning in PEG/salt aqueous two-phase systems has been investigated. PEG 4000/phosphate systems in the presence of 0% w/w and 8.8% w/w NaCl have been evaluated using amyloglucosidase, subtilisin, and trypsin inhibitor. Also, a PEG 4000/phosphate system with 3% w/w NaCl was u...

journal_title：Biotechnology and bioengineering

authors： Schmidt AS,Andrews BA,Asenjo JA

更新日期：1996-06-20 00:00:00

abstract：:As the therapeutic monoclonal antibody (mAb) market continues to grow, optimizing production processes is becoming more critical in improving efficiencies and reducing cost-of-goods in large-scale production. With the recent trends of increasing cell culture titers from upstream process improvements, downstream capaci...

journal_title：Biotechnology and bioengineering

authors： Dizon-Maspat J,Bourret J,D'Agostini A,Li F

更新日期：2012-04-01 00:00:00

abstract：:Two-dimensional image analysis was applied to counting, sizing, and density determinations of granules in full-scale and laboratory-scale upflow anaerobic sludge blanket (UASB) digesters. An advantage of this technique for monitoring laboratory-scale digester sludge is the small amount of material required for analysi...

journal_title：Biotechnology and bioengineering

authors： Dudley BT,Howgrave-Graham AR,Bruton AG,Wallis FM

更新日期：1993-07-01 00:00:00

abstract：:Penicillinase (beta-lactamase I, EC 3.5.2.6) secreted by Bacillus cereus, strain 569/H, was covalently attached to aminoethyl cellulose via glutaraldehyde. The immobilized derivative shows increased thermostability and decreased susceptibility to conformational changes induced by certain substrates of penicillinase. T...

journal_title：Biotechnology and bioengineering

authors： Klemes Y,Citri N

更新日期：1979-05-01 00:00:00

abstract：:A titration bioassay, designed to accurately determine the activity of acetoclastic methanogens, is described that also allows evaluation of inhibition due to potential toxicants on the active biomass. The instrument is made of a pH-stat connected to an anaerobic batch reactor. Acetate is blended and mixed with anaero...

journal_title：Biotechnology and bioengineering

authors： Rozzi A,Castellazzi L,Speece RE

更新日期：2002-01-05 00:00:00

abstract：:A sequencing batch membrane biofilm reactor (SBMBfR) was developed for simultaneous carbon, nitrogen, and phosphorus removal from wastewater. This reactor was composed of two functional parts: (1) a gas-permeable membrane on which a nitrifying biofilm formed and (2) a bulk solution in which bacteria, mainly denitrifyi...

journal_title：Biotechnology and bioengineering

authors： Terada A,Yamamoto T,Tsuneda S,Hirata A

更新日期：2006-07-05 00:00:00

abstract：:C-terminal lysine (C-K) variants are commonly observed in therapeutic monoclonal antibodies and recombinant proteins. Heterogeneity of C-K residues is believed to result from varying degree of proteolysis by endogenous carboxypeptidase(s) during cell culture production. The achievement of batch-to-batch culture perfor...

journal_title：Biotechnology and bioengineering

authors： Luo J,Zhang J,Ren D,Tsai WL,Li F,Amanullah A,Hudson T

更新日期：2012-09-01 00:00:00

abstract：:The increasing accessibility of mass isotopomer data via GC-MS and NMR technology has necessitated the use of a systematic and reliable method to take advantage of such data for flux analysis. Here we applied a nonlinear, optimization-based method to study substrate metabolism in cardiomyocytes using (13)C data from p...

journal_title：Biotechnology and bioengineering

authors： Vo TD,Palsson BO

更新日期：2006-12-05 00:00:00

abstract：:Three cell lines, that is, the human breast cancer cell line (MCF-7) and the human mammary epithelial cell line (S-1) and its malignant form (T4-2) were embedded in a reconstituted basement membrane (Matrigel) that had 20-nL pyramid-shaped silicon microstructures. The proliferative behavior of the MCF-7 cells was depe...

journal_title：Biotechnology and bioengineering

authors： Torisawa YS,Nashimoto Y,Yasukawa T,Shiku H,Matsue T

更新日期：2007-06-15 00:00:00

abstract：:The performance and microbial communities of three differently inoculated biotrickling filters removing dimethyl sulfide (DMS) were compared. The biotrickling filters were inoculated with Thiobacillus thioparus TK-m (THIO), sludge (HANDS) and sludge + T. thioparus TK-m + Hyphomicrobium VS (HANDS++), respectively. The ...

journal_title：Biotechnology and bioengineering

authors： Sercu B,Boon N,Beken SV,Verstraete W,Van Langenhove H

更新日期：2007-03-01 00:00:00

abstract：:Constitutive activation of Wnt signaling plays an important role in colorectal and liver tumorigenesis. Cell-based assays using synthetic TCF/LEF (T-cell factor/lymphoid enhancer factor) reporters, as readouts of β-catenin/TCF-dependent transcriptional activity, have contributed greatly to the discovery of small molec...

journal_title：Biotechnology and bioengineering

authors： Yamaguchi K,Zhu C,Ohsugi T,Yamaguchi Y,Ikenoue T,Furukawa Y

更新日期：2017-12-01 00:00:00

abstract：:Two attractive features of ELISA are the specificity of antibody-antigen recognition and the sensitivity achieved by enzymatic amplification. This report describes the development of a non-enzymatic molecular recognition platform adaptable to point-of-care clinical settings and field detection of biohazardous material...

journal_title：Biotechnology and bioengineering

authors： Stone GP,Mernaugh R,Haselton FR

更新日期：2005-09-20 00:00:00

abstract：:A second generation flow contraction device was developed and modeled which allows cells to be subjected to well-defined hydrodynamic forces. Studies were conducted with this system on wild-type Chinese Hamster Ovary cells (CHO-K1) and a strain of CHO cells which expresses the human Bcl-2 triangle gene (CHO-bcl-2). In...

journal_title：Biotechnology and bioengineering

authors： Mollet M,Godoy-Silva R,Berdugo C,Chalmers JJ

更新日期：2007-11-01 00:00:00

abstract：:A novel technique for immobilization of Candida rugosa lipase onto anionic colloidal gas aphrons (CGAs) is described. CGAs are spherical microbubbles (10-100 microm) composed of an inner gas core surrounded by a surfactant shell. In this initial study, greater than 80% lipase (w/w) was effectively retained on the CGAs...

journal_title：Biotechnology and bioengineering

authors： O'Connell PJ,Varley J

更新日期：2001-08-05 00:00:00

abstract：:It has long been established that UVC light is a very effective method for inactivating pathogens in a fluid, yet the application of UVC irradiation to modern biotechnological processes is limited by the intrinsic short penetration distance of UVC light in optically dense protein solutions. This experimental and numer...

journal_title：Biotechnology and bioengineering

authors： Ren Y,Crump CM,Mackley MM,Li Puma G,Reis NM

更新日期：2016-07-01 00:00:00

abstract：:Biofiltration of air polluted by volatile organic compounds is now recognized by the industrial and research communities as an effective and viable alternative to standard environmental technologies. Whereas many studies have focused on solid/liquid/gas biofilters, there have been fewer reports on waste air treatment ...

journal_title：Biotechnology and bioengineering

authors： Erable B,Goubet I,Lamare S,Legoy MD,Maugard T

更新日期：2004-04-05 00:00:00

abstract：:D-Methionine was converted to L-methionine in a reaction system where four enzymes were used. D-amino acid oxidase (D-AAO) from Arthrobacter protophormiae was used for the complete conversion of D-methionine to 2-oxo-4-methylthiobutyric acid. Catalase was added to prevent 2-oxo-4-methylthiobutyric acid decarboxylation...

journal_title：Biotechnology and bioengineering

authors： Findrik Z,Vasić-Racki D

更新日期：2007-12-01 00:00:00

abstract：:The production of heterologous proteins by secretion from cellular hosts is an important determinant for the cost of biotherapeutics. A single-cell analytical method called microengraving was used to examine the heterogeneity in secretion by the methylotrophic yeast Pichia pastoris. We show that constitutive secretion...

journal_title：Biotechnology and bioengineering

authors： Love KR,Panagiotou V,Jiang B,Stadheim TA,Love JC

更新日期：2010-06-01 00:00:00

abstract：:Design of 3D scaffolds that can facilitate proper survival, proliferation, and differentiation of progenitor cells is a challenge for clinical applications involving large connective tissue defects. Cell migration within such scaffolds is a critical process governing tissue integration. Here, we examine effects of sca...

journal_title：Biotechnology and bioengineering

authors： Peyton SR,Kalcioglu ZI,Cohen JC,Runkle AP,Van Vliet KJ,Lauffenburger DA,Griffith LG

更新日期：2011-05-01 00:00:00

abstract：:Overexpression of the cyclin-dependent kinase inhibitor p27 and exposure to low temperature (30 degrees C) represent two strategies to establish controlled proliferation processes for production of therapeutic proteins using Chinese hamster ovary (CHO) cells. Here we analyze the effect of growth inhibition on the qual...

journal_title：Biotechnology and bioengineering

authors： Kaufmann H,Mazur X,Marone R,Bailey JE,Fussenegger M

更新日期：2001-03-20 00:00:00

abstract：:L-Threonine, a kind of essential amino acid, has numerous applications in food, pharmaceutical, and aquaculture industries. Fermentative l-threonine production from glucose has been achieved in Escherichia coli. However, there are still several limiting factors hindering further improvement of l-threonine productivity...

journal_title：Biotechnology and bioengineering

authors： Liu J,Li H,Xiong H,Xie X,Chen N,Zhao G,Caiyin Q,Zhu H,Qiao J

更新日期：2019-01-01 00:00:00

abstract：:Trichoderma reesei (QM 9414) produced cellulase in continuous culture, on media containing xylose (1%) supplemented with sorbose (0.3%) to induce cellulase production. Maximum cell mass of 4.54 kg/m(3) occurred at pH 4.0 and a dilution rate of 0.0391 h(-1) where residual substrate was 0.43 kg/m(3), but no cellulase wa...

journal_title：Biotechnology and bioengineering

authors： Schafner DW,Toledo RT

更新日期：1992-04-05 00:00:00

abstract：:Aggregates of Escherichia coli confined within hollowfiber reactors were either formed in place by culturing cells within the reactors, or were prepared by injecting a cell suspension that had been concentrated by centrifugation. The effective diffusive permeability of an uncharged nonreacting tracer, nitrous oxide, w...

journal_title：Biotechnology and bioengineering

authors： Libicki SB,Salmon PM,Robertson CR

更新日期：1988-06-20 00:00:00

abstract：:Metabolically active resting (i.e., nongrowing) bacterial cells have a high potential in cofactor-dependent redox biotransformations. Where growing cells require carbon and energy for biomass production, resting cells can potentially exploit their metabolism more efficiently for redox biocatalysis allowing higher spec...

journal_title：Biotechnology and bioengineering

authors： Julsing MK,Kuhn D,Schmid A,Bühler B

更新日期：2012-05-01 00:00:00

abstract：:Derivatives of Escherichia coli C were engineered to produce primarily succinate or malate in mineral salts media using simple fermentations (anaerobic stirred batch with pH control) without the addition of plasmids or foreign genes. This was done by a combination of gene deletions (genetic engineering) and metabolic ...

journal_title：Biotechnology and bioengineering

authors： Jantama K,Haupt MJ,Svoronos SA,Zhang X,Moore JC,Shanmugam KT,Ingram LO

更新日期：2008-04-01 00:00:00

abstract：:Nanotechnology is becoming increasingly important for products used in our daily lives, such as the masses of titanium dioxide nanoparticle agglomerates (TiO(2) NPs) used in the pharmaceutical industry, for cosmetic products, or for pigments. Meanwhile, a serious lack of detailed information concerning the interaction...

journal_title：Biotechnology and bioengineering

authors： Chen P,Kanehira K,Sonezaki S,Taniguchi A

更新日期：2012-12-01 00:00:00

abstract：:The microscopic morphology of Trichoderma reesei QM 9414, growing in submerged culture, was studied by image analysis. The morphology was characterized by the total hyphal length, the total number of tips, the number of actively growing tips, and the length of the main hypha. To describe the growth of a single myceliu...

journal_title：Biotechnology and bioengineering

authors： Lejeune R,Nielsen J,Baron GV

更新日期：1995-09-05 00:00:00

abstract：:A high-rate anaerobic digestion of food waste leachate were tested using intermittent continuously stirred tank reactors (iCSTRs) to evaluate how severe ammonia inhibition could be mitigated with internal dilution strategy, and to identify how bacterial and archaeal community respond in genus and species level. Experi...

journal_title：Biotechnology and bioengineering

authors： Yun YM,Kim DH,Cho SK,Shin HS,Jung KW,Kim HW

更新日期：2016-09-01 00:00:00

abstract：:A dynamic method is proposed for k(l)a measurement in aerated and agitated reactors, in which a change in the total pressure in the reactor by approximately 20% leads to a simultaneous change in the oxygen concentration in all the bubbles in the dispersion. This procedure suppresses the influence of nonideal mixing of...

journal_title：Biotechnology and bioengineering

authors： Linek V,Benes P,Vacek V

更新日期：1989-05-01 00:00:00

abstract：:The ferrous-iron oxidation kinetics of a bacterial culture consisting predominantly of Leptospirillum ferrooxidans were studied in continuous-flow bioreactors. The bacterial culture was fed with a salts solution containing 12 g/L ferrous-iron, at dilution rates ranging from 0.01 to 0.06 l/h, and temperatures ranging f...

journal_title：Biotechnology and bioengineering

authors： Breed AW,Dempers CJ,Searby GE,Gardner MN,Rawlings DE,Hansford GS

更新日期：1999-10-05 00:00:00