Further improvement of phosphite dehydrogenase thermostability by saturation mutagenesis.

abstract：:Co-culture of hepatocytes or hepatocyte spheroids with the supporting NIH3T3 in a 3D microcapsule formed with a hybrid natural/synthetic matrix has led to enhanced hepatocyte functions. We investigated the mechanism of the functional enhancement in co-culture with respect to the contributions of soluble factors and di...

journal_title：Biotechnology and bioengineering

authors： Chia SM,Lin PC,Yu H

更新日期：2005-03-05 00:00:00

abstract：:Due to its availability, low-price, and high degree of reduction, glycerol has become an attractive carbon source for the production of fuels and reduced chemicals. Using the platform we have established from the identification of key pathways mediating fermentative metabolism of glycerol, this work reports the engine...

journal_title：Biotechnology and bioengineering

authors： Clomburg JM,Gonzalez R

更新日期：2011-04-01 00:00:00

abstract：:Biomass autoflocculation in outdoor algal cultures was found to be associated with increases of culture pH levels, due to CO(2) consumption by the algal photosynthetic activity. Under these alkaline conditions, some medium chemical ions precipitated together with the algal biomass. The chemical substances involved wit...

journal_title：Biotechnology and bioengineering

authors： Sukenik A,Shelef G

更新日期：1984-02-01 00:00:00

abstract：:Beta defensins are antimicrobial peptides (AMPs) with a broad spectrum antimicrobial behavior against pathogens while having minimal tendency to incur pathogen resistance. Human β-defensin 28 (hBD28) is a strongly cationic AMP and hence hypothesized to be highly effective in permeabilizing negatively-charged pathogen ...

journal_title：Biotechnology and bioengineering

authors： Tay DK,Rajagopalan G,Li X,Chen Y,Lua LH,Leong SS

更新日期：2011-03-01 00:00:00

abstract：:The in vitro generation of cell-based three dimensional (3D) nerve tissue is an attractive subject to improve graft survival and integration into host tissue for neural tissue regeneration or to model biological events in stem cell differentiation. Although 3D organotypic culture strategies are well established for 3D...

journal_title：Biotechnology and bioengineering

authors： Kim BC,Jun SM,Kim SY,Kwon YD,Choe SC,Kim EC,Lee JH,Kim J,Suh JF,Hwang YS

更新日期：2017-04-01 00:00:00

abstract：:Fed-batch cultures of recombinant microorganisms have attracted attention as they can separate cell growth stage from cloned-gene expression phase during fermentations. In this work, the effect of different glucose feeding strategies on cell growth and cloned gene expression was studied during aerobic fed-batch fermen...

journal_title：Biotechnology and bioengineering

authors： Patkar A,Seo JH

更新日期：1992-06-05 00:00:00

abstract：:Strains from Trichoderma reesei have been used for cellulase production with a long history. It has been well known that cellulase biosynthesis by the fungal species is controlled through regulators, and elucidation of their regulation network is of great importance for engineering T. reesei with robust cellulase prod...

journal_title：Biotechnology and bioengineering

authors： Meng QS,Zhang F,Liu CG,Zhao XQ,Bai FW

更新日期：2020-06-01 00:00:00

abstract：:The mean sizes and size distributions of air bubbles and viscous castor oil drops were studied in a salt-rich aqueous solution (medium), first separately, and then simultaneously as a three-phase system. The dispersion was created in a 150-mm-diameter stirred tank equipped with a Rushton turbine, and the sizes were me...

journal_title：Biotechnology and bioengineering

authors： Galindo E,Pacek AW,Nienow AW

更新日期：2000-07-20 00:00:00

abstract：:A new method is described for the immobilization of biologically active proteins onto several types of organic polymer beads. First, the soluble protein is modified by reaction with an excess of a hydrophobic imidoester, for example methyl 4-phenyl-butyrimidate, at ca. pH 9 and 0 degrees . Excess imidoester and side p...

journal_title：Biotechnology and bioengineering

authors： Ampon K,Means GE

更新日期：1988-08-20 00:00:00

abstract：:Chinese hamster ovary (CHO) cells are the predominant host cell line for the production of biopharmaceuticals, a growing industry currently worth more than $188 billion USD in global sales. CHO cells undergo programmed cell death (apoptosis) following different stresses encountered in cell culture, such as substrate l...

journal_title：Biotechnology and bioengineering

authors： Henry MN,MacDonald MA,Orellana CA,Gray PP,Gillard M,Baker K,Nielsen LK,Marcellin E,Mahler S,Martínez VS

更新日期：2020-04-01 00:00:00

abstract：:Membrane separation and chromatographic technologies are regarded as an attractive alternative to conventional academic small-scale ultracentrifugation procedures used for retrovirus purification. However, despite the increasing demands for purified retroviral vector preparations, new chromatography adsorbents with hi...

journal_title：Biotechnology and bioengineering

authors： Segura MM,Kamen A,Trudel P,Garnier A

更新日期：2005-05-20 00:00:00

abstract：:The study reports on the development of a bioreactor for the production of alpha-keto acids from D,L- or D-amino acids using Rhodotorula gracilis D-amino acid oxidase. D-amino acid oxidase was co-immobilized with catalase on Affi-Gel 10 matrix, and the reactor was operated as a continuous-stirred tank reactor (CSTR) o...

journal_title：Biotechnology and bioengineering

authors： Butò S,Pollegioni L,D'Angiuro L,Pilone MS

更新日期：1994-12-01 00:00:00

abstract：:Increasing demand for petroleum has stimulated industry to develop sustainable production of chemicals and biofuels using microbial cell factories. Fatty acids of chain lengths from C6 to C16 are propitious intermediates for the catalytic synthesis of industrial chemicals and diesel-like biofuels. The abundance of gen...

journal_title：Biotechnology and bioengineering

authors： Tee TW,Chowdhury A,Maranas CD,Shanks JV

更新日期：2014-05-01 00:00:00

abstract：:A thermodynamic framework (UNIQUAC model with temperature dependent parameters) is applied to model the salt-induced protein crystallization equilibrium, i.e., protein solubility. The framework introduces a term for the solubility product describing protein transfer between the liquid and solid phase and a term for th...

journal_title：Biotechnology and bioengineering

authors： Agena SM,Pusey ML,Bogle ID

更新日期：1999-07-20 00:00:00

abstract：:The influence of fluid-dynamic conditions on the yield of Phaeodactylum tricornutum microalgal cultures was analyzed in two stages: first, the influence of air flow rate; second, the influence of using fluid-moving pumps for recirculating the culture. With respect to the air flow rate, the yield of the cultures increa...

journal_title：Biotechnology and bioengineering

authors： Alías CB,García-Malea López MC,Acién Fernández FG,Ferníndez Sevilla JM,García Sánchez JL,Molina Grima E

更新日期：2004-09-20 00:00:00

abstract：:We describe a method by which the degree of bubble saturation can be determined by measuring the velocity of single bubbles at different heights from the bubble source in pure water containing increasing concentrations of surfactants. The highest rising velocities were measured in pure water. Addition of surfactants c...

journal_title：Biotechnology and bioengineering

authors： Jordan M,Eppenberger HM,Sucker H,Widmer F,Einsele A

更新日期：1994-03-15 00:00:00

abstract：:Many enzymes or fluorescent proteins produced in Escherichia coli are enzymatically active or fluorescent respectively when deposited as inclusion bodies. The occurrence of insoluble but functional protein species with native-like secondary structure indicates that solubility and conformational quality of recombinant ...

journal_title：Biotechnology and bioengineering

authors： Martínez-Alonso M,García-Fruitós E,Villaverde A

更新日期：2008-12-15 00:00:00

abstract：:Numerous studies on poly γ-d-glutamicacid (γ-PGA) production have investigated terrestrial renewable sources for reducing production costs, but there are no studies using waste marine resources so far. We aimed to develop a cost-effective production method of γ-d-PGA by Bacillus sp. SJ-10 using green macroalgae (Ulva ...

journal_title：Biotechnology and bioengineering

authors： Kim JH,Lee JM,Jang WJ,Park HD,Kim YO,Kim CH,Kong IS

更新日期：2019-07-01 00:00:00

abstract：:Abnormal airway mucus presents a significant challenge for inhalational drug delivery. Recognizing the thick and tenacious airway mucus seen in the cystic fibrosis (CF) patients as a critical barrier to effective drug delivery, both into the mucus layer itself as well as across that layer to the underlying airway epit...

journal_title：Biotechnology and bioengineering

authors： Yang Y,Tsifansky MD,Shin S,Lin Q,Yeo Y

更新日期：2011-06-01 00:00:00

abstract：:The reconstruction of functioning human tissues ex vivo is becoming an important part of biotechnology. There are compelling scientific, clinical, and biotechnological reasons for fully or partially reconstituting human tissues such as skin, bone marrow, and liver ex vivo. In particular, bone marrow is a tissue of muc...

journal_title：Biotechnology and bioengineering

authors： Koller MR,Palsson BO

更新日期：1993-10-01 00:00:00

abstract：:The effect of hydrogen and carbon dioxide partial pressure on the growth of the extremely thermophilic archaebacterium Pyrodictium brockii at 98 degrees C was investigated. Previous work with this bacterium has been done using an 80:20 hydrogen-carbon dioxide gas phase with a total pressure of 4 atm; no attempt has be...

journal_title：Biotechnology and bioengineering

authors： Parameswaran AK,Schicho RN,Soisson JP,Kelly RM

更新日期：1988-08-05 00:00:00

abstract：:Currently in America the biofuel ethanol is primarily being produced by the dry grind technique to obtain the starch contained in the corn grains and subsequently subjected to fermentation. This so-called 1st generation technology has two setbacks; first the lingering debate whether its life cycle contributes to a red...

journal_title：Biotechnology and bioengineering

authors： Brehmer B,Bals B,Sanders J,Dale B

更新日期：2008-09-01 00:00:00

abstract：:The second messenger cyclic dimeric (3'→5') GMP (cyclic di-GMP or c-di-GMP) has been implicated in the transition between motile and sessile lifestyles in bacteria. In this study, we demonstrate that biofilm formation, cellular aggregation or flocculation, and cellular buoyancy are under the control of c-di-GMP in Syn...

journal_title：Biotechnology and bioengineering

authors： Agostoni M,Waters CM,Montgomery BL

更新日期：2016-02-01 00:00:00

abstract：:The Waterloo Fast Pyrolysis Process (WFPP) can produce an organic liquid high in levoglucosan (1, 6-anhydro-beta-D-glucopyranose) content from suitably pretreated lignocellulosics. A variety of fungi and yeasts were screened for their ability to utilize and ferment this organic liquid. To enhance its fermentability, t...

journal_title：Biotechnology and bioengineering

authors： Prosen EM,Radlein D,Piskorz J,Scott DS,Legge RL

更新日期：1993-08-05 00:00:00

abstract：:Regenerating damaged tissue interfaces remains a significant clinical challenge, requiring recapitulation of the structure, composition, and function of the native enthesis. In the ligament-to-bone interface, this region transitions from ligament to fibrocartilage, to calcified cartilage and then to bone. This gradati...

journal_title：Biotechnology and bioengineering

authors： Harris E,Liu Y,Cunniffe G,Morrissey D,Carroll S,Mulhall K,Kelly DJ

更新日期：2017-10-01 00:00:00

abstract：:A complete, integrated process for the production of an innovative formulation of penicillin G acylase from Providencia rettgeri(rPAC(P.rett))of industrial applicability is reported. In order to improve the yield of rPAC, the clone LN5.5, carrying four copies of pac gene integrated into the genome of Pichia pastoris, ...

journal_title：Biotechnology and bioengineering

authors： Senerovic L,Stankovic N,Spizzo P,Basso A,Gardossi L,Vasiljevic B,Ljubijankic G,Tisminetzky S,Degrassi G

更新日期：2006-02-05 00:00:00

abstract：:The use of microalgal biomass for metal pollutant bioremediation might be improved by genetic engineering to modify the selectivity or capacity of metal biosorption. A plant cadmium (Cd) and zinc (Zn) transporter (AtHMA4) was used as a transgene to increase the ability of Chlamydomonas reinhardtii to tolerate 0.2 mM C...

journal_title：Biotechnology and bioengineering

authors： Ibuot A,Webster RE,Williams LE,Pittman JK

更新日期：2020-10-01 00:00:00

abstract：:Metabolic carbon labelling experiments enable a large amount of extracellular fluxes and intracellular carbon isotope enrichments to be measured. Since the relation between the measured quantities and the unknown intracellular metabolic fluxes is given by bilinear balance equations, flux determination from this data s...

journal_title：Biotechnology and bioengineering

authors： Wiechert W,Siefke C,de Graaf AA,Marx A

更新日期：1997-07-05 00:00:00

abstract：:The application of rational design in reallocating metabolic flux to accumulate desired chemicals is always restricted by the native regulatory network. In this study, recombinant Pichia pastoris was constructed for malic acid production from sole methanol through rational redistribution of metabolic flux. Different m...

journal_title：Biotechnology and bioengineering

authors： Guo F,Dai Z,Peng W,Zhang S,Zhou J,Ma J,Dong W,Xin F,Zhang W,Jiang M

更新日期：2021-01-01 00:00:00

abstract：:Continuous propionic acid fermentations of lactate by Propionibacterium acidipropionici were studied in spiral wound fibrous bed bioreactors. Cells were imobilized by natural attachment to fiber surfaces and entrapment in the void volume within the fibrous matrix. A high cell density of approximately 37 g/L was attain...

journal_title：Biotechnology and bioengineering

authors： Lewis VP,Yang ST

更新日期：1992-08-05 00:00:00