Regulation of biofilm formation and cellular buoyancy through modulating intracellular cyclic di-GMP levels in engineered cyanobacteria.

abstract：:Continous culture experiments with the obligatory methanotroph, Methylosinus trichosporium OB3b, were conducted to study the whole-cell methane monooxygenase (MMO) and nitrogenase activities in a nitrate minimal salts medium under oxygen-limited conditions with methane as the carbone source. The important variables in...

journal_title：Biotechnology and bioengineering

authors： Shah NN,Park S,Taylor RT,Droege MW

更新日期：1992-09-01 00:00:00

abstract：:The design and operation of an industrial penicillin-V deacylation reactor is simulated, using a kinetic expression and mass transport parameters for the immobilized enzyme particles which were determined experimentally in a previous study. It is desirable to use a series of equalsized plug flow reactors with pH contr...

journal_title：Biotechnology and bioengineering

authors： Karlsen LG,Villadsen J

更新日期：1984-12-01 00:00:00

abstract：:A novel multivariable control strategy is developed for alcohol (ethanol and n-pentanol) concentrations in the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(HB-co-HV), a biodegradable polymer by Paracoccus denitrificans ATCC 1774. This controller, which is developed to control the mole fraction of P(HB...

journal_title：Biotechnology and bioengineering

authors： Chanprateep S,Abe N,Shimizu H,Yamane T,Shioya S

更新日期：2001-07-20 00:00:00

abstract：:A fuzzy-logic light attenuation model was developed and validated for a stirred-tank photobioreactor. Based on this model, local light intensities were used to calculate local specific growth rates of the cyanobacteria Synechococcus PCC 7942. The light regime for maximization of biomass space-time yield in a batch pro...

journal_title：Biotechnology and bioengineering

authors： Franco-Lara E,Havel J,Peterat F,Weuster-Botz D

更新日期：2006-12-20 00:00:00

abstract：:This article demonstrates the feasibility of a novel process concept for the remediation of PCB contaminated soil. The proposed process consists of PCB extraction from soil using solid polymer beads, followed by biodegradation of the extracted PCBs in a solid-liquid two-phase partitioning bioreactor (TPPB), where PCBs...

journal_title：Biotechnology and bioengineering

authors： Rehmann L,Daugulis AJ

更新日期：2008-04-01 00:00:00

abstract：:Saccharomyces cerevisiae (yeast) cells were employed as a source of alcohol dehydrogenase in the NAD(+)-to-NADH reaction. The cells were immobilized in calcium alginate monofilament fibers and used in a biological reactor. The alginate could not be heat sterilized since temperatures above 80 degrees C caused the polym...

journal_title：Biotechnology and bioengineering

authors： Ibbotson PG,Spedding PL,Otterburn MS

更新日期：1990-10-05 00:00:00

abstract：:Cell-based tissue engineering is limited by the size of cell-containing constructs that can be successfully cultured in vitro. This limit is largely a result of the slow diffusion of molecules such as oxygen into the interior of three-dimensional scaffolds in static culture. Bioreactor culture has been shown to overco...

journal_title：Biotechnology and bioengineering

authors： Yeatts AB,Geibel EM,Fears FF,Fisher JP

更新日期：2012-09-01 00:00:00

abstract：:Enterokinase and recombinant enterokinase light chain (rEK(L)) have been used widely to cleave fusion proteins with the target sequence of (Asp)(4)-Lys. In this work, we show that their utility as a site-specific cleavage agent is compromised by sporadic cleavage at other sites, albeit at low levels. Further degradati...

journal_title：Biotechnology and bioengineering

authors： Choi SI,Song HW,Moon JW,Seong BL

更新日期：2001-12-20 00:00:00

abstract：:Invertase from Candida utilis was immobilized on porous cellulose beads by an ionic-quanidino bond. The immobilized invertase showed optimum activity between pH 4.0 and 5.4, while the free enzyme had a sharp optimum at pH 4.1. Both temperature profiles were fairly similar up to 55 degrees C. However, above this temper...

journal_title：Biotechnology and bioengineering

authors： Dickensheets PA,Chen LF,Tsao GT

更新日期：1977-03-01 00:00:00

abstract：:A newly designed batch calorimeter was used to investigate the degradability of some celluloses having varying degrees of crystallinity. The PTC of an aerobic culture of Cellulomonas uda ATCC 21399 obtained revealed a diauxic growth which is attributed to the presence of hemicellulose contaminating Avicel and MN300 ce...

journal_title：Biotechnology and bioengineering

authors： Dermoun Z,Belaich JP

更新日期：1985-07-01 00:00:00

abstract：:Nitrifying bacteria, cyanobacteria, and algae are important microorganisms in open pond wastewater treatment systems. Nitrification involving the sequential oxidation of ammonia to nitrite and nitrate, mainly due to autotrophic nitrifying bacteria, is essential to biological nitrogen removal in wastewater and global n...

journal_title：Biotechnology and bioengineering

authors： Choi O,Das A,Yu CP,Hu Z

更新日期：2010-12-15 00:00:00

abstract：:A packed granular activated carbon (GAC) biobed, inoculated with the ethane-degrading strain Mycobacterium E3, was used to study ethene removal from a synthetic waste gas. Ethene, for which the dimensionless partition coefficient for an air-water system at 20 degrees C is about 7.6, was used as a model compound for po...

journal_title：Biotechnology and bioengineering

authors： De Heyder B,Overmeire A,Van Langenhove H,Verstraete W

更新日期：1994-08-20 00:00:00

abstract：:Coastal bermudagrass was pretreated by a low-temperature ammonia fiber explosion (AFEX) process, which soaked the grass in liquid ammonia and then explosively released the pressure. Saccharifying enzymes were systematically applied to the AFEX-treated grass corresponding to low, medium, and high loadings of cellulase/...

journal_title：Biotechnology and bioengineering

authors： Holtzapple MT,Ripley EP,Nikolaou M

更新日期：1994-11-05 00:00:00

abstract：:The ferrous-iron oxidation kinetics of a bacterial culture consisting predominantly of Leptospirillum ferrooxidans were studied in continuous-flow bioreactors. The bacterial culture was fed with a salts solution containing 12 g/L ferrous-iron, at dilution rates ranging from 0.01 to 0.06 l/h, and temperatures ranging f...

journal_title：Biotechnology and bioengineering

authors： Breed AW,Dempers CJ,Searby GE,Gardner MN,Rawlings DE,Hansford GS

更新日期：1999-10-05 00:00:00

abstract：:Protein switches have a variety of potential applications in biotechnology and medicine that motivate efforts to accelerate their development. Switches can be built by the proper fusion of two proteins with the prerequisite input and output functions. However, the exact fusion geometry for switch creation, which typic...

journal_title：Biotechnology and bioengineering

authors： Tullman J,Nicholes N,Dumont MR,Ribeiro LF,Ostermeier M

更新日期：2016-04-01 00:00:00

abstract：:Metabolically active resting (i.e., nongrowing) bacterial cells have a high potential in cofactor-dependent redox biotransformations. Where growing cells require carbon and energy for biomass production, resting cells can potentially exploit their metabolism more efficiently for redox biocatalysis allowing higher spec...

journal_title：Biotechnology and bioengineering

authors： Julsing MK,Kuhn D,Schmid A,Bühler B

更新日期：2012-05-01 00:00:00

abstract：:Effective and efficient breakdown of lignocellulosic biomass remains a primary barrier for its use as a feedstock for renewable transportation fuels. A more detailed understanding of the material properties of biomass slurries during conversion is needed to design cost-effective conversion processes. A series of enzym...

journal_title：Biotechnology and bioengineering

authors： Roche CM,Dibble CJ,Knutsen JS,Stickel JJ,Liberatore MW

更新日期：2009-10-01 00:00:00

abstract：:Microaerobic (oxygen-limited) conditions are critical for inducing many important microbial processes in industrial or environmental applications. At very low oxygen concentrations, however, the process performance often suffers from technical limitations. Available dissolved oxygen measurement techniques are not sens...

journal_title：Biotechnology and bioengineering

authors： Carius L,Rumschinski P,Faulwasser T,Flockerzi D,Grammel H,Findeisen R

更新日期：2014-04-01 00:00:00

abstract：:Three-dimensional cell culture models, such as spheroids, can be used in the process of the development of new anticancer agents because they are able to closely mimic the main features of human solid tumors, namely their structural organization, cellular layered assembling, hypoxia, and nutrient gradients. These prop...

journal_title：Biotechnology and bioengineering

authors： Nunes AS,Barros AS,Costa EC,Moreira AF,Correia IJ

更新日期：2019-01-01 00:00:00

abstract：:This study examines protein adsorption behavior and the effects of mobile phase modifiers in multimodal chromatographic systems. Chromatography results with a diverse protein library indicate that multimodal and ion exchange resins have markedly different protein binding behavior and selectivity. NMR results corrobora...

journal_title：Biotechnology and bioengineering

authors： Holstein MA,Parimal S,McCallum SA,Cramer SM

更新日期：2012-01-01 00:00:00

abstract：:Physical damage of animal cells in suspension culture, due to stirring and sparging, is coupled with complex metabolic responses. Nylon microcapsules, therefore, were used as a physical model to study the mechanisms of damage in a stirred bioreactor and in a bubble column. Microcapsule breaskage folowed first-order ki...

journal_title：Biotechnology and bioengineering

authors： Lu GZ,Gray MR,Thompson BG

更新日期：1992-12-05 00:00:00

abstract：:The desired product of bioprocesses is often produced in particulate form, either as an inclusion body (IB) or as a crystal. Particle harvesting is then a crucial and attractive form of product recovery. Because the liquid phase often contains other bioparticles, such as cell debris, whole cells, particulate biocataly...

journal_title：Biotechnology and bioengineering

authors： van Hee P,Hoeben MA,van der Lans RG,van der Wielen LA

更新日期：2006-07-05 00:00:00

abstract：:Short-chain carboxylic acids generated by various mixed- or pure-culture fermentation processes have been considered valuable precursors for production of bioalcohols. While conversion of carboxylic acids into alcohols is routinely performed with catalytic hydrogenation or with strong chemical reducing agents, here, a...

journal_title：Biotechnology and bioengineering

authors： Perez JM,Richter H,Loftus SE,Angenent LT

更新日期：2013-04-01 00:00:00

abstract：:Diffusion of phenol through a biofilm attached to activated carbon particles was investigated. The biofilm was grown on activated carbon particles in a draft-tube three-phase fluidized-bed bioreactor operating in a fed-batch mode. It was found that phenol did not adsorb on the biofilm and that the diffusion coefficien...

journal_title：Biotechnology and bioengineering

authors： Fan LS,Leyva-Ramos R,Wisecarver KD,Zehner BJ

更新日期：1990-02-05 00:00:00

abstract：:Engineered human T-cells are a promising therapeutic modality for cancer immunotherapy. T-cells expressing chimeric antigen receptors combined with additional genes to enhance T-cell proliferation, survival, or tumor targeting may further improve efficacy but require multiple stable gene transfer events. Methods are t...

journal_title：Biotechnology and bioengineering

authors： Kacherovsky N,Liu GW,Jensen MC,Pun SH

更新日期：2015-07-01 00:00:00

abstract：:Phaeodactylum tricornutum is a widely studied diatom and has been proposed as a source of oil and polyunsaturated fatty acids (PUFA), particularly eicosapentaenoic acid (EPA). Recent studies indicate that lipid accumulation occurs under nutritional stress. Aim of this research was to determine how changes in nitrogen ...

journal_title：Biotechnology and bioengineering

authors： Rodolfi L,Biondi N,Guccione A,Bassi N,D'Ottavio M,Arganaraz G,Tredici MR

更新日期：2017-10-01 00:00:00

abstract：:Penicillinase (beta-lactamase I, EC 3.5.2.6) secreted by Bacillus cereus, strain 569/H, was covalently attached to aminoethyl cellulose via glutaraldehyde. The immobilized derivative shows increased thermostability and decreased susceptibility to conformational changes induced by certain substrates of penicillinase. T...

journal_title：Biotechnology and bioengineering

authors： Klemes Y,Citri N

更新日期：1979-05-01 00:00:00

abstract：:Axial and radial oxygen depletion are believed to be critical scale-limiting factors in the design of cell culture hollow fiber bioreactors. A mathematical analysis of oxygen depletion has been performed in order to develop effectiveness factor plots to aid in the scaling of hollow fiber bioreactors with cells immobil...

journal_title：Biotechnology and bioengineering

authors： Piret JM,Cooney CL

更新日期：1991-01-05 00:00:00

abstract：:Polymers produced by horseradish-peroxidase-catalyzed coupling of phenols have been explored as potential substitutes for phenol-formaldehyde resins. To overcome low substrate solubilities and product molecular weights in water, enzymatic polymerizations in aqueous-organic mixtures have been examined. Peroxidase vigor...

journal_title：Biotechnology and bioengineering

authors： Dordick JS,Marletta MA,Klibanov AM

更新日期：1987-07-01 00:00:00

abstract：:The ability of algae and bacteria to accumulate heavy metals from the surrounding environment is a widely recognized phenomenon that has a number of important implications. This work reports on the development of a quantitative model that addresses the basic mechanisms inherent in many uptake processes. The model post...

journal_title：Biotechnology and bioengineering

authors： Ting YP,Lawson F,Prince IG

更新日期：1989-10-05 00:00:00