Abstract:
:A packed granular activated carbon (GAC) biobed, inoculated with the ethane-degrading strain Mycobacterium E3, was used to study ethene removal from a synthetic waste gas. Ethene, for which the dimensionless partition coefficient for an air-water system at 20 degrees C is about 7.6, was used as a model compound for poorly water soluble gaseous pollutants. In a first mode or operation, the GAC biobed was sprinkled intermittently and the waste gas influent was continuously pre-humidified, establishing relatively moist conditions (water content >40% to 45%). A volumetric ethene removal rate of 0.382 kg COD x m(-3) x d(-1) (0.112 kg ethene x m(-3) x d(-1)) was obtained for an influent concentration of 125 ppm, a superficial waste gas velocity of 3.6E-3 m x s(-1) and a pseudo residence time of 45 s. However, in the second mode of operation, omitting the pre-humidification of the waste gas influent and establishing a "dry" biobed (water content <40% to 45%), and thus obtaining better mass transfer to the biofilm, the ethene removal could be doubled for otherwise comparable operating parameters. Furthermore, under decreased wetting and for the given experimental conditions (influent concentration 125 to 816 ppm, waste gas superficial velocity 3.0E-3 m x s(-1), pseudo waste gas residence time 43 s), the ethene removal was not limited by mass transfer of ethene through the water layer covering the biofilm.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
De Heyder B,Overmeire A,Van Langenhove H,Verstraete Wdoi
10.1002/bit.260440511subject
Has Abstractpub_date
1994-08-20 00:00:00pages
642-8issue
5eissn
0006-3592issn
1097-0290journal_volume
44pub_type
杂志文章abstract::In Part II, the process utility of a thermotolerant methylotrophic bacterium is evaluated with respect to its dynamic response when various substrate or nutrient pulses are imposed on it while growing under steady-state conditions in a chemostat. The pulses investigated were methanol pulses on methanol-, methanol/form...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260360811
更新日期:1990-10-20 00:00:00
abstract::The scope in improving enzyme productivities from the cellulose fermentation process is examined in laboratory-scale fermentors. The maximum productivity (30 IU/liter hr) is attained in a continuous-culture process with cell recycle using modified medium containing 0.5% cellulose. Optimum dilution rate and recycle rat...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260210213
更新日期:1979-02-01 00:00:00
abstract::High hydrostatic pressure (HHP) stabilized glucose oxidase (GOx) against thermal inactivation. The apparent first-order kinetics of inactivation of GOx were investigated at 0.1-300 MPa and 58.8-80.0°C. At 240 MPa and 74.5°C, GOx inactivated at a rate 50 times slower than at atmospheric pressure at the same temperature...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26185
更新日期:2017-03-01 00:00:00
abstract::Mass balances were performed on an alkaline lysis operation for the primary recovery of supercoiled plasmid DNA as part of a process for plasmid gene preparation. Escherichia coli DH5alpha/pSVbeta was cultured in defined medium by fed-batch fermentation and harvested at the end of the exponential phase. Alkaline lysis...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10166
更新日期:2002-03-30 00:00:00
abstract::The compound 5-hydroxymethylfurfural (HMF) has attracted much attention due to its versatility as an important bio-based platform chemical. Here, we engineered Raoultella ornithinolytica BF60 as a whole-cell biocatalyst for a highly efficient synthesis of 2,5-furandicarboxylic acid (FDCA) from HMF. Specifically, vario...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26725
更新日期:2018-09-01 00:00:00
abstract::In order to evaluate the sensitivity of hepatocellular cultures to variations in both substrate stiffness and bioactive ligand presentation, hepatocytes were cultured on differentially compliant polyacrylamide gel discs functionalized with varying amounts of the ECM ligand, fibronectin (FN). Subconfluent cell cultures...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20328
更新日期:2005-02-05 00:00:00
abstract::Based upon the general characteristics of the optimal feed rate profiles presented in an earlier article, efficient computational algorithms have been developed for fed-batch fermentation processes described by four or less mass balance equations. These algorithms make computations of optimal substrate feed rate profi...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260280915
更新日期:1986-09-01 00:00:00
abstract::A uniquely simple model is developed to describe the NO(3) (-) and NO(2) (-) concentration profiles within a dentrification fluidized bed biological reactor. This simple model is compared to experimental data, and to a more complex model similar to those previously proposed in the literature. The simple model fits the...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19970620)54:6<543::AID-BIT
更新日期:1997-06-20 00:00:00
abstract::An extractive acetonobutylic fermentation process is developed by integrating bioproduction, Ultrafiltration, and distillation, providing simultaneous retention of biomass, selective removal of inhibitors from the permeate, as well as separation and purification of acetone-butanol-ethanol solvents. Successive batch fe...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260350903
更新日期:1990-04-15 00:00:00
abstract::A second generation flow contraction device was developed and modeled which allows cells to be subjected to well-defined hydrodynamic forces. Studies were conducted with this system on wild-type Chinese Hamster Ovary cells (CHO-K1) and a strain of CHO cells which expresses the human Bcl-2 triangle gene (CHO-bcl-2). In...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21476
更新日期:2007-11-01 00:00:00
abstract::Two general models for batch simultaneous enzymatic and microbial reaction (SEMR) processes are presented, the second derived from and simpler than the first and accounting for enzyme denaturation. Using the second model and parameter values from the literature, simulation was used to examine a range of enzyme additio...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260371114
更新日期:1991-05-01 00:00:00
abstract::Lignin, one of the major components of lignocellulosic biomass, plays an inhibitory role on the enzymatic hydrolysis of cellulose. This work examines the role of lignin in pretreated hardwood, where extents of cellulose hydrolysis decrease, rather than increase with increasing severity of liquid hot water pretreatment...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章,评审
doi:10.1002/bit.25349
更新日期:2015-02-01 00:00:00
abstract::Catalytic activity of lipases (from Rhizopus arrhizus, Canadida rugosa, and Pseudomonas sp. was studied in organic media, mainly diisopropyl ether. The effect of water activity (a(w)) on V(max) showed that the enzyme activity in general increased with increasing amounts of water for the three enzymes. This was shown b...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19970905)55:5<798::AID-BIT
更新日期:1997-09-05 00:00:00
abstract::The diffusion of viruses toward cells is a limiting step of the infection process. To be modeled correctly, this step must be evaluated in combination with the adsorption of the virus to the cell surface, which is a rapid but reversible step. In this paper, the recombinant adenovirus (rAd) diffusion and its adsorption...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21340
更新日期:2007-09-01 00:00:00
abstract::A submerged anaerobic dynamic membrane bioreactor (AnDMBR) was operated for treatment of concentrated wastewater. The dynamic membrane (DM) or cake layer was characterized on its physicochemical and biological composition and the role of the DM layer in treatment and filtration performances was assessed. The results s...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25841
更新日期:2016-04-01 00:00:00
abstract::In order to effectively treat obesity, it must be better understood at the cellular level with respect to metabolic state and environmental stress. However, current two-dimensional (2D) in vitro cell culture methods do not represent the in vivo adipose tissue appropriately due to the absence of complex architecture an...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25099
更新日期:2014-01-01 00:00:00
abstract::Fungal morphology in many filamentous fungal fermentations leads to high broth viscosity which limits oxygen mass transfer, and often results in reduced productivity. The objective in this study was to determine if a simple, fed-batch, process strategy-pulsed addition of limiting-carbon source-could be used to reduce ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10548
更新日期:2003-04-05 00:00:00
abstract::Somatic embryogenesis is among the most promising means of large scale plant micropropagation. The development of somatic embryos is characterized by their morphological changes. Embryos in culture usually exhibit high heterogeneity and abnormality. As conventional microscopic observation is laborious and subjective, ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19960405)50:1<65::AID-BIT8
更新日期:1996-04-05 00:00:00
abstract::Lipid productivity and fatty acid composition are important metrics for the production of high quality biodiesel from algae. Our previous results showed that co-culturing the green alga Chlorella minutissima with Escherichia coli under high-substrate mixotrophic conditions enhanced both culture growth and crude lipid ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25609
更新日期:2015-09-01 00:00:00
abstract::RNA sequencing (RNASeq) has been widely used to associate alterations in Chinese hamster ovary (CHO) cell gene expression with bioprocess phenotypes; however, alternative messenger RNA (mRNA) splicing, has thus far, received little attention. In this study, we utilized RNASeq for transcriptomic analysis of a monoclona...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27365
更新日期:2020-08-01 00:00:00
abstract::Overexpression of recombinant proteins in Escherichia coli often leads to a severe growth retardation of the host cells. The phage T7 promoter phi10 in a pET vector was utilized to express human superoxide dismutase. Induction with IPTG lead to an increase in protein content and cell size and a termination of cell div...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10353
更新日期:2002-10-05 00:00:00
abstract::The present study evaluated the influence of water activity and lactose concentration on the synthesis of galactooligosaccharides (GOS), by means of a hyperthermophilic beta-glycosidase in an organic system. The production of GOS gradually grew as water activity increased in the reaction system; later, their synthesis...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20824
更新日期:2006-04-20 00:00:00
abstract::The glycosylation of pharmaglycoproteins from recombinant cell lines can be affected by an uncontrolled accumulation of ammonium in the medium. Glucosamine-6-phosphate isomerase (GPI) has been proposed as the key enzyme responsible for elevating the intracellular UDP-N-acetylhexosamine pool (UDPGNAc) by accepting ammo...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19991020)65:2<192::aid-bit
更新日期:1999-10-20 00:00:00
abstract::As the therapeutic monoclonal antibody (mAb) market continues to grow, optimizing production processes is becoming more critical in improving efficiencies and reducing cost-of-goods in large-scale production. With the recent trends of increasing cell culture titers from upstream process improvements, downstream capaci...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24377
更新日期:2012-04-01 00:00:00
abstract::Co-Immobilization of dextransucrase (DS) and dextranase (DN) into calcium alginate includes the co-entrapment of soluble DS and adsorbed DN. DS converts sucrose into dextran, which is the substrate for DN, so that isomalto-oligosaccharides (IMOs) are follow-up products of dextran hydrolysis. The boundary conditions fo...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21810
更新日期:2008-07-01 00:00:00
abstract::Hairy root cultures of Catharanthus roseus were established by infection of seedlings with Agrobacterium rhizogenes 15834. Approximately 150 transformants from four different. C. roseus cultivars were screened for desirable traits in growth and indole alkaloid production. Five hairy root clones grew well in liquid cul...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260410511
更新日期:1993-03-05 00:00:00
abstract::A proteinase isolated from Thermus RT41a was immobilized to controlled pore glass beads and was used in the free and immobilized forms for peptide synthesis. The observed maximum yield was the same in both cases. a number of dipeptides were produced from amino acid esters and amides. The best acyl components, from tho...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260440311
更新日期:1994-07-01 00:00:00
abstract::The C-terminal lysine variation is commonly observed in biopharmaceutical monoclonal antibodies. This modification can be important since it is found to be sensitive to the production process. The methods commonly used to probe this charge variation, including IEF, cIEF, ion-exchange chromatography, and LC-MS, were ev...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21855
更新日期:2008-08-15 00:00:00
abstract::Exchange of enzyme-bound H(2)O with T(2)O in aqueous solution followed by freeze drying provided tritiated water bound to chymotrypsin, subtilisin Carlsberg, and horseradish peroxidase. The desorption of T(2)O from these enzymes suspended in various organic solvents showed that all three enzymes lost enzyme-bound wate...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260390405
更新日期:1992-02-20 00:00:00
abstract::Abnormal airway mucus presents a significant challenge for inhalational drug delivery. Recognizing the thick and tenacious airway mucus seen in the cystic fibrosis (CF) patients as a critical barrier to effective drug delivery, both into the mucus layer itself as well as across that layer to the underlying airway epit...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.23046
更新日期:2011-06-01 00:00:00