Glucose oxidase stabilization against thermal inactivation using high hydrostatic pressure and hydrophobic modification.


:High hydrostatic pressure (HHP) stabilized glucose oxidase (GOx) against thermal inactivation. The apparent first-order kinetics of inactivation of GOx were investigated at 0.1-300 MPa and 58.8-80.0°C. At 240 MPa and 74.5°C, GOx inactivated at a rate 50 times slower than at atmospheric pressure at the same temperature. The apparent activation energy of inactivation at 300 MPa was 281.0 ± 17.4 kJ mol-1 or 1.3-fold smaller than for the inactivation at atmospheric pressure (378.1 ± 25.6 kJ mol-1 ). The stabilizing effect of HHP was greatest at 74.5°C, where the activation volume of 57.0 ± 12.0 cm3  mol-1 was highest compared to all other studied temperatures. Positive apparent activation volumes for all the treatment temperatures confirmed that HHP favors GOx stabilization. A second approach to increase GOx stability involved crosslinking with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) and either aniline or benzoate. The modified enzyme remained fully active with only slight increases in KM (1.3-1.9-fold increases for aniline and benzoate modification, respectively). The thermal stability of GOx increased by 8°C with aniline modification, while it decreased by 0.9°C upon modification with benzoate. Biotechnol. Bioeng. 2017;114: 516-525. © 2016 Wiley Periodicals, Inc.


Biotechnol Bioeng


Halalipour A,Duff MR Jr,Howell EE,Reyes-De-Corcuera JI




Has Abstract


2017-03-01 00:00:00












  • Increased metal tolerance and bioaccumulation of zinc and cadmium in Chlamydomonas reinhardtii expressing a AtHMA4 C-terminal domain protein.

    abstract::The use of microalgal biomass for metal pollutant bioremediation might be improved by genetic engineering to modify the selectivity or capacity of metal biosorption. A plant cadmium (Cd) and zinc (Zn) transporter (AtHMA4) was used as a transgene to increase the ability of Chlamydomonas reinhardtii to tolerate 0.2 mM C...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ibuot A,Webster RE,Williams LE,Pittman JK

    更新日期:2020-10-01 00:00:00

  • Random mutagenesis of global transcription factor cAMP receptor protein for improved osmotolerance.

    abstract::The naturally existing microbial hosts can rarely satisfy industrial requirements, thus there has always been an intense effort in strain engineering to meet the needs of these bioprocesses. Here, in this work, we want to prove the concept that engineering global transcription factor cAMP receptor protein (CRP) of Esc...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Zhang H,Chong H,Ching CB,Jiang R

    更新日期:2012-05-01 00:00:00

  • Identification of key residues modulating the stereoselectivity of nitrile hydratase toward rac-mandelonitrile by semi-rational engineering.

    abstract::Optically pure compounds are important in the synthesis of fine chemicals. Using directed evolution of enzymes to obtain biocatalysts that can selectively produce high-value chiral chemicals is often time-, money-, and resource-intensive; traditional semi-rational designs based on structural data and docking experimen...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Cheng Z,Peplowski L,Cui W,Xia Y,Liu Z,Zhang J,Kobayashi M,Zhou Z

    更新日期:2018-03-01 00:00:00

  • Itaconic acid production from glycerol using Escherichia coli harboring a random synonymous codon-substituted 5'-coding region variant of the cadA gene.

    abstract::Aspergillus terreus cadA, encoding cis-aconitate decarboxylase, is an essential gene for itaconic acid (IA) biosynthesis, but it is primarily expressed as insoluble aggregates in most industrial hosts. This has been a hurdle for the development of recombinant strategies for IA production. Here, we created a library of...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Jeon HG,Cheong DE,Han Y,Song JJ,Choi JH

    更新日期:2016-07-01 00:00:00

  • Kinetics of the enzymatic hydrolysis of cellulose.

    abstract::Enzymatic hydrolysis of cellulose for sugar production offers advantages of higher conversion, minimal by-product formation, low energy requirements, and mild operating conditions over other chemical conversions. The development of a kinetic model, based on observable, macroscopic properties of the overall system, is ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Wald S,Wilke CR,Blanch HW

    更新日期:1984-03-01 00:00:00

  • Biofabrication of antibodies and antigens via IgG-binding domain engineered with activatable pentatyrosine pro-tag.

    abstract::We report the assembly of seven different antibodies (and two antigens) into functional supramolecular structures that are specifically designed to facilitate integration into devices using entirely biologically based bottom-up fabrication. This is enabled by the creation of an engineered IgG-binding domain (HG3T) wit...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Wu HC,Shi XW,Tsao CY,Lewandowski AT,Fernandes R,Hung CW,DeShong P,Kobatake E,Valdes JJ,Payne GF,Bentley WE

    更新日期:2009-06-01 00:00:00

  • Application of a mechanistic model as a tool for on-line monitoring of pilot scale filamentous fungal fermentation processes-The importance of evaporation effects.

    abstract::A mechanistic model-based soft sensor is developed and validated for 550L filamentous fungus fermentations operated at Novozymes A/S. The soft sensor is comprised of a parameter estimation block based on a stoichiometric balance, coupled to a dynamic process model. The on-line parameter estimation block models the cha...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Mears L,Stocks SM,Albaek MO,Sin G,Gernaey KV

    更新日期:2017-03-01 00:00:00

  • Biofilm parameters influencing biocide efficacy.

    abstract::The influence of biofilm areal cell density, species composition, and the presence of abiotic particles on the disinfection and removal of bacterial biofilms by monochloramine was investigated. Mono- and binary population biofilms of Pseudomonas aeruginosa and Klebsiella pneumoniae were grown on stainless-steel slides...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Srinivasan R,Stewart PS,Griebe T,Chen CI,Xu X

    更新日期:1995-06-20 00:00:00

  • A defined co-culture of Geobacter sulfurreducens and Escherichia coli in a membrane-less microbial fuel cell.

    abstract::Wastewater-fed microbial fuel cells (MFCs) are a promising technology to treat low-organic carbon wastewater and recover part of the chemical energy in wastewater as electrical power. However, the interactions between electrochemically active and fermentative microorganisms cannot be easily studied in wastewater-fed M...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Bourdakos N,Marsili E,Mahadevan R

    更新日期:2014-04-01 00:00:00

  • Feasibility of enzymatic hydrolysis and alcoholic fermentation of starch contained in buffalo gourd (Cucurbita foetidissima) roots.

    abstract::The suitability of using annually grown, carrot-sized buffalo gourd (Cucurbita foetidissima) roots as a feedstock for alcoholic fermentation was explored. Roots grown in 1982 and 1983 were slurried, dextrinized and saccharified using Takathermtrade mark and Diazymetrade mark (commercial enzymes manufactured by Miles L...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Scheerens JC,Kopplin MJ,Abbas IR,Nelson JM,Gathman AC,Berry JW

    更新日期:1987-03-01 00:00:00

  • Biochemical characterization of a novel thermo-halo-tolerant GH5 endoglucanase from a thermal spring metagenome.

    abstract::A novel endoglucanase gene, celM , was cloned from a thermal spring metagenome. The gene was expressed in Escherichia coli, and the protein was extracted and purified. The protein catalyzed the hydrolysis of amorphous cellulose in a wide range of temperatures, 30-95°C, with optimal activity at 80°C. It was able to tol...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Joshi N,Kaushal G,Singh SP

    更新日期:2021-01-06 00:00:00

  • Directed evolution of xylose specific transporters to facilitate glucose-xylose co-utilization.

    abstract::A highly active xylose specific transporter without glucose inhibition is highly desirable in cost-effective production of biofuels from lignocellulosic biomass. However, currently available xylose specific transporters suffer from low overall activity and most are inhibited by glucose. In this study, we applied a dir...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Wang M,Yu C,Zhao H

    更新日期:2016-03-01 00:00:00

  • Cholestyramine as a promising, strong anion exchange resin for direct capture of genetic biomarkers from raw pancreatic fluids.

    abstract::The ability to capture cell-free DNA from the gastrointestinal tract, in a minimally invasive manner, could enhance our ability to diagnose gastrointestinal disease, or gain a better understanding of the spatial mapping of the intestinal microbiota. We, therefore, sought to identify a class of capture agents that coul...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Hilmer AJ,Jeffrey RB,Park WG,Khosla C

    更新日期:2017-04-01 00:00:00

  • Three-dimensional biofilm model with individual cells and continuum EPS matrix.

    abstract::An innovative type of biofilm model is derived by combining an individual description of microbial particles with a continuum representation of the biofilm matrix. This hybrid model retains the advantages of each approach, while providing a more realistic description of the temporal development of biofilm structure in...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Alpkvist E,Picioreanu C,van Loosdrecht MC,Heyden A

    更新日期:2006-08-05 00:00:00

  • Immobilized enzymes in reverse micelles: studies with gel-entrapped trypsin and alpha-chymotrypsin in AOT reverse micelles.

    abstract::Trypsin and alpha-chymotrypsin were immobilized by gelentrapment in polyacrylamide cross-linked with N,N(1)-methylenebisacrylamide. The immobilized enzymes are catalytically efficient in suspensions of reverse micelles formed in isooctane by bis(2-ethylhexyl) sodium sulfosuccinate (AOT) and water. Both entrapped enzym...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Fadnavis NW,Luisi PL

    更新日期:1989-04-20 00:00:00

  • High concentrations of clavulanic acid but not of its degradation products decrease glycerol consumption and oxygen uptake rates in cultures of Streptomyces clavuligerus.

    abstract::Product inhibition of biological production systems is a widely observed phenomenon with prominent implications for the design and ultimately the success of biotechnological processes. In order to investigate whether such effects could limit the maximal concentrations in the production of the beta-lactamase inhibitor ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Brethauer S,Held M,Panke S

    更新日期:2008-06-15 00:00:00

  • Process performance and product quality in an integrated continuous antibody production process.

    abstract::Continuous manufacturing is currently being seriously considered in the biopharmaceutical industry as the possible new paradigm for producing therapeutic proteins, due to production cost and product quality related benefits. In this study, a monoclonal antibody producing CHO cell line was cultured in perfusion mode an...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Karst DJ,Steinebach F,Soos M,Morbidelli M

    更新日期:2017-02-01 00:00:00

  • Metabolic modeling for predicting VFA production from protein-rich substrates by mixed-culture fermentation.

    abstract::Proteinaceous organic wastes are suitable substrates to produce high added-value products in anaerobic mixed-culture fermentations. In these processes, the stoichiometry of the biotransformation depends highly on operational conditions such as pH or feeding characteristics and there are still no tools that allow the p...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Regueira A,Lema JM,Carballa M,Mauricio-Iglesias M

    更新日期:2020-01-01 00:00:00

  • Influence of uncertainties in pH, pMg, activity coefficients, metabolite concentrations, and other factors on the analysis of the thermodynamic feasibility of metabolic pathways.

    abstract::Thermodynamic feasibility analysis (TFA) has been used as a tool capable of providing additional constraints to the mass balance-based methods of analysis of metabolic networks (e.g., flux balance analysis). Several publications have recently appeared in which TFA of different metabolic pathways from relatively simple...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Vojinović V,von Stockar U

    更新日期:2009-07-01 00:00:00

  • Biodegradation of PCBs in two-phase partitioning bioreactors following solid extraction from soil.

    abstract::This article demonstrates the feasibility of a novel process concept for the remediation of PCB contaminated soil. The proposed process consists of PCB extraction from soil using solid polymer beads, followed by biodegradation of the extracted PCBs in a solid-liquid two-phase partitioning bioreactor (TPPB), where PCBs...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Rehmann L,Daugulis AJ

    更新日期:2008-04-01 00:00:00

  • In situ determination of kinetic parameters for biofilms: Isolation and characterization of oligotrophic biofilms.

    abstract::This article develops and utilizes an in situ technique to estimate the Monod half-maximum rate concentration, K(s) and the maximum specific utilization rate constant, k, for biofilms. The technique employs a curve-matching method with kinetic results from several short-term experiments with completely mixed biofilm r...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Rittmann BE,Crawford L,Tuck CK,Namkung E

    更新日期:1986-11-01 00:00:00

  • Integrated reactor concepts for the enzymatic kinetic synthesis of cephalexin.

    abstract::Integrated process concepts for enzymatic cephalexin synthesis were investigated by our group, and this article focuses on the integration of reactions and product removal during the reactions. The last step in cephalexin production is the enzymatic kinetic coupling of activated phenylglycine (phenylglycine amide or p...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Schroën CG,Nierstrasz VA,Bosma R,Kroon PJ,Tjeerdsma PS,DeVroom E,VanderLaan JM,Moody HM,Beeftink HH,Janssen AE,Tramper J

    更新日期:2002-10-20 00:00:00

  • Continuous conversion of D-xylose to ethanol by immobilized pachysolen tannophilus.

    abstract::The yeast Pachysolen tannophilus was entrapped in calcium alginate beads to ferment D-xylose on a continous basis in the presence of high cell densities. Experimental operating variables included the feed D-xylose concentration, the dilution rate, and the fermentor biomass concentration. Under favorable operating cond...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Slininger PJ,Bothast RJ,Black LT,McGhee JE

    更新日期:1982-10-01 00:00:00

  • Membrane-attached biofilms for VOC wastewater treatment. II: Effect of biofilm thickness on performance.

    abstract::This article reports a study of the performance of membrane-attached biofilms grown in a single tube extractive membrane bioreactor (STEMS) used for the treatment of a synthetic wastewater containing a toxic VOC (1,2-dichloroethane [DCE]). Mass balances show that complete mineralization of DCE was achieved, and that t...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Dos Santos LM,Livingston AG

    更新日期:1995-07-05 00:00:00

  • Concentration of baboon endogenous virus in large-scale production by use of hollow-fiber ultrafiltration technology.

    abstract::A process is described in which the baboon endogenous virus (BaEV) is produced under optimum conditions in cell culture, and concentrated by hollow-fiber ultrafiltration technology under conditions of large-scale production. This system has advantages over conventional systems in that the flow rate is increased 2.5-fo...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Weiss SA

    更新日期:1980-01-01 00:00:00

  • Kinetic resolution of racemic glycidyl butyrate using a multiphase membrane enzyme reactor: experiments and model verification.

    abstract::A laboratory-scale multiphase hollow fiber membrane reactor was employed to investigate the lipase-catalyzed enzymatic resolution of racemic glycidyl butyrate. A mathematical formulation was developed to simulate the performance of this system. Model parameters were determined independently (except the effective rate ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Wu DR,Cramer SM,Belfort G

    更新日期:1993-04-25 00:00:00

  • Forecasting and control of lactate bifurcation in Chinese hamster ovary cell culture processes.

    abstract::Biomanufacturing exhibits inherent variability that can lead to variation in performance attributes and batch failure. To help ensure process consistency and product quality the development of predictive models and integrated control strategies is a promising approach. In this study, a feedback controller was develope...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Schmitt J,Downey B,Beller J,Russell B,Quach A,Lyon D,Curran M,Mulukutla BC,Chu C

    更新日期:2019-09-01 00:00:00

  • Application of an electric DNA-chip for the expression analysis of bioprocess-relevant marker genes of Bacillus subtilis.

    abstract::The knowledge of critical process-relevant genes can be used for an improved control of bioprocesses. So far bioprocess-relevant marker genes can be analyzed by established expression analysis methods only off-line. In this study, an alternative approach for a potential at-line monitoring of gene expression during bio...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Jürgen B,Barken KB,Tobisch S,Pioch D,Wümpelmann M,Hecker M,Schweder T

    更新日期:2005-11-05 00:00:00

  • Oxygen uptake rates and liver-specific functions of hepatocyte and 3T3 fibroblast co-cultures.

    abstract::Bioartificial liver (BAL) devices have been developed to treat patients undergoing acute liver failure. One of the most important parameters to consider in designing these devices is the oxygen consumption rate of the seeded hepatocytes which are known to have oxygen consumption rates 10 times higher than most other c...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Cho CH,Park J,Nagrath D,Tilles AW,Berthiaume F,Toner M,Yarmush ML

    更新日期:2007-05-01 00:00:00

  • Surfactant-Induced hydrogen production in cyanobacteria.

    abstract::Addition of Tween 85 to aqueous suspensions of Anabaena variabilis induced photosynthetic evolution of hydrogen over a time span of several weeks: As much as 148 nmol H(2)/h . mg dry weight was produced in the first week by a suspension containing 4.2 mg dry weight of cells and 77 mM Tween 85. The chemical structure o...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Famiglietti M,Hochkoeppler A,Luisi PL

    更新日期:1993-10-01 00:00:00