Characteristics and role of dynamic membrane layer in anaerobic membrane bioreactors.

abstract：:Anaerobic methanethiol (MT) degradation by mesophilic (30 degrees C) alkaliphilic (pH 10) communities was studied in a lab-scale Upflow Anaerobic Sludge Bed (UASB) reactor inoculated with a mixture of sediments from the Wadden Sea (The Netherlands), Soap Lake (Central Washington), and Russian soda lakes. MT degradatio...

journal_title：Biotechnology and bioengineering

authors： van Leerdam RC,Bonilla-Salinas M,de Bok FA,Bruning H,Lens PN,Stams AJ,Janssen AJ

更新日期：2008-11-01 00:00:00

abstract：:Non-viral delivery of cell-impermeant drugs and DNA in vivo has traditionally relied upon either chemical or physical stress applied directly to target tissues. Physical methods typically use contact between an applicator, or electrode, and the target tissue and may involve patient discomfort. To overcome contact-depe...

journal_title：Biotechnology and bioengineering

authors： Connolly RJ,Lopez GA,Hoff AM,Jaroszeski MJ

更新日期：2009-12-01 00:00:00

abstract：:Two types of polyethylenimine-coated glass microbeads (13-44 micrometer) were synthesized and used for the immobilization of glucose oxidase from Aspergillus niger and catalase from A. niger and beef liver. The two types of beads were distinguishable by differences in their surface topography. Immobilizations were per...

journal_title：Biotechnology and bioengineering

authors： Wasserman BP,Hultin HO,Jacobson BS

更新日期：1980-02-01 00:00:00

abstract：:Animal-derived serum is an essential media supplement for mammalian cells in cell culture. For a number of reasons including cost, regulatory concerns, lot inconsistency, potential contamination with adventitious agents, and down-stream processing it is desirable to eliminate the use of serum. Existing protocols desig...

journal_title：Biotechnology and bioengineering

authors： Jaluria P,Konstantopoulos K,Betenbaugh M,Shiloach J

更新日期：2008-04-15 00:00:00

abstract：:Potentiometric and amperometric measurements were made with microbial fuel cells containing E. coli or yeast as the anodic reducing agent and glucose as the oxidizable substrate. The catalytic effects of thionine and resorufin on the anode reaction were investigated. Results on the potentiometry, polarization, and cou...

journal_title：Biotechnology and bioengineering

authors： Bennetto HP,Stirling JL,Tanaka K,Vega CA

更新日期：1983-02-01 00:00:00

abstract：:Screening tests of different marine algae biomas types revealed a high passive biosorptive uptake of lead up to 270 mg Pb/g of biomass in some brown marine algae. Members of the order Fucales perfomed particularly well in this descending sequence: Fucus > Ascophyllum > Sargassum. Although decreasing the swelling of we...

journal_title：Biotechnology and bioengineering

authors： Holan ZR,Volesky B

更新日期：1994-05-01 00:00:00

abstract：:Many biochemical processes consist of a sequence of operations for which optimal operating conditions (setpoints) have to be determined. If such optimization is performed for each operation separately with respect to objectives defined for each operation individually, overall process performance is likely to be subopt...

journal_title：Biotechnology and bioengineering

authors： Groep ME,Gregory ME,Kershenbaum LS,Bogle ID

更新日期：2000-02-05 00:00:00

abstract：:As part of an investigation into the use of biological nitrogen fixation for fertilizer ammonia production, continuous culture studies of respiration and nitrogen fixation in the aerobic bacteria Azotobacter vinelandii under oxygen-limited conditions were conducted. Respiration and growth rates followed Monod forms wi...

journal_title：Biotechnology and bioengineering

authors： Diluccio RC,Kirwan DJ

更新日期：1984-01-01 00:00:00

abstract：:Clostridium carboxidivorans P7 is one of three microbial catalysts capable of fermenting synthesis gas (mainly CO, CO(2) , and H(2) ) to produce the liquid biofuels ethanol and butanol. Gasification of feedstocks to produce synthesis gas (syngas), followed by microbial conversion to solvents, greatly expands the diver...

journal_title：Biotechnology and bioengineering

authors： Ukpong MN,Atiyeh HK,De Lorme MJ,Liu K,Zhu X,Tanner RS,Wilkins MR,Stevenson BS

更新日期：2012-11-01 00:00:00

abstract：:Catalytic activity of lipases (from Rhizopus arrhizus, Canadida rugosa, and Pseudomonas sp. was studied in organic media, mainly diisopropyl ether. The effect of water activity (a(w)) on V(max) showed that the enzyme activity in general increased with increasing amounts of water for the three enzymes. This was shown b...

journal_title：Biotechnology and bioengineering

authors： Wehtje E,Adlercreutz P

更新日期：1997-09-05 00:00:00

abstract：:Mammalian cell cultures typically exhibit an energy inefficient phenotype characterized by the consumption of large quantities of glucose and the concomitant production of large quantities of lactate. Under certain conditions, mammalian cells can switch to a more energy efficient state during which lactate is consumed...

journal_title：Biotechnology and bioengineering

authors： Martínez VS,Dietmair S,Quek LE,Hodson MP,Gray P,Nielsen LK

更新日期：2013-02-01 00:00:00

abstract：:Rifamycin B is an important polyketide antibiotic used in the treatment of tuberculosis and leprosy. We present results on medium optimization for Rifamycin B production via a barbital insensitive mutant strain of Amycolatopsis mediterranei S699. Machine-learning approaches such as Genetic algorithm (GA), Neighborhood...

journal_title：Biotechnology and bioengineering

authors： Bapat PM,Wangikar PP

更新日期：2004-04-20 00:00:00

abstract：:Lipase (EC 3.1.1.3., from Pseudomonas sp.) was entrapped in collagen membrane containing liquid crystal (4-methoxybenzilidene-4'-n-butylaniline). The activity of the lipase-liquid crystal membrane at an applied voltage of 4 V was 3.4 compared to a membrane tested without imposition of an external electric field. A lin...

journal_title：Biotechnology and bioengineering

authors： Karube I,Yugeta Y,Suzuki S

更新日期：1977-10-01 00:00:00

abstract：:The transesterification reaction of N-acetyl-L-phenylalanine ethyl ester with 1-propanol catalyzed by alpha-chymotrypsin was examined in the ionic liquids 1-butyl-3-methylimidazolium hexafluorophosphate ([bmim][PF(6)]) and 1-octyl-3-methylimidazolium hexafluorophosphate ([omim][PF(6)]), and in combination with supercr...

journal_title：Biotechnology and bioengineering

authors： Laszlo JA,Compton DL

更新日期：2001-10-20 00:00:00

abstract：:Highly substituted polychlorinated biphenyls (PCBs) are known to be very resistant to aerobic biodegradation, particularly the initial attack by biphenyl dioxygenase. Functional evolution of the substrate specificity of biphenyl dioxygenase was demonstrated by DNA shuffling and staggered extension process (StEP) of th...

journal_title：Biotechnology and bioengineering

authors： Brühlmann F,Chen W

更新日期：1999-06-05 00:00:00

abstract：:We have used a novel approach in conjunction with flow cytometry to quantify the biological heterogeneity of populations of the ciliate Tetrahymena pyriformis. It was found that the rate of particle uptake of exponentially growing cells is not uniform among cells and partially correlated with cell size. The physiologi...

journal_title：Biotechnology and bioengineering

authors： Hatzis C,Srienc F,Fredrickson AG

更新日期：1994-03-05 00:00:00

abstract：:A production process for B. thuringiensis (Bt) bioinsecticides was designed in detail, including alternative batch, low-density fed-batch (LDFB), and high-density fed-batch (HDFB) fermentation configurations. Capital and operating costs, as well as profitability based on simple rate of return, were performed using a p...

journal_title：Biotechnology and bioengineering

authors： Rowe GE,Margaritis A

更新日期：2004-05-20 00:00:00

abstract：:Abnormal airway mucus presents a significant challenge for inhalational drug delivery. Recognizing the thick and tenacious airway mucus seen in the cystic fibrosis (CF) patients as a critical barrier to effective drug delivery, both into the mucus layer itself as well as across that layer to the underlying airway epit...

journal_title：Biotechnology and bioengineering

authors： Yang Y,Tsifansky MD,Shin S,Lin Q,Yeo Y

更新日期：2011-06-01 00:00:00

abstract：:In order to effectively treat obesity, it must be better understood at the cellular level with respect to metabolic state and environmental stress. However, current two-dimensional (2D) in vitro cell culture methods do not represent the in vivo adipose tissue appropriately due to the absence of complex architecture an...

journal_title：Biotechnology and bioengineering

authors： Turner PA,Harris LM,Purser CA,Baker RC,Janorkar AV

更新日期：2014-01-01 00:00:00

abstract：:Understanding the regulatory networks which control specific macrophage phenotypes is essential in identifying novel targets to correct macrophage mediated clinical disorders, often accompanied by inflammatory events. Since mesenchymal stromal cells (MSCs) have been shown to play key roles in regulating immune functio...

journal_title：Biotechnology and bioengineering

authors： Barminko JA,Nativ NI,Schloss R,Yarmush ML

更新日期：2014-11-01 00:00:00

abstract：:Use of cellulase for denim washing is a standard eco-friendly technique to achieve desirable appearance and softness for cotton fabrics and denims. But enzymatic washing of denim till date involved acid cellulase (Trichoderma reesei) and neutral cellulase (Humicola isolens) the use of which has a drawback of backstain...

journal_title：Biotechnology and bioengineering

authors： Anish R,Rahman MS,Rao M

更新日期：2007-01-01 00:00:00

abstract：:Electron paramagnetic resonance spectroscopy is used to observe hydroxyl radicals produced by an atmospheric pressure nonthermal plasma device at distances greater than 1 m from the discharge. The plasma device is an indirect treatment setup with closed loop airflow and hydrogen peroxide additives that is effective in...

journal_title：Biotechnology and bioengineering

authors： Plimpton SR,Gołkowski M,Mitchell DG,Austin C,Eaton SS,Eaton GR,Gołkowski C,Voskuil M

更新日期：2013-07-01 00:00:00

abstract：:Enzymatic hydrolysis of arabinoxylan is an important prerequisite for the utilization of hemicellulose for ethanol fermentation or for making the low calorie sweetener xylitol by catalytic hydrogenation of the generated xylose. This study focus on cloning and characterization of two industrial relevant beta-xylosidase...

journal_title：Biotechnology and bioengineering

authors： Rasmussen LE,Sørensen HR,Vind J,Viksø-Nielsen A

更新日期：2006-08-05 00:00:00

abstract：:A semimechanistic multi-reaction kinetic model was developed to describe the enzymatic hydrolysis of a lignocellulosic biomass, creeping wild ryegrass (CWR; Leymus triticoides). This model incorporated one homogeneous reaction of cellobiose-to-glucose and two heterogeneous reactions of cellulose-to-cellobiose and cell...

journal_title：Biotechnology and bioengineering

authors： Zheng Y,Pan Z,Zhang R,Jenkins BM

更新日期：2009-04-15 00:00:00

abstract：:The morphology of mycelial fungi in liquid culture effects culture rheology and this in turn may affect product yield. It is therefore important to understand how environmental factors influence mycelial morphology and this paper describes the effect of dilution rate on two strains of Fusarium graminearum, the relativ...

journal_title：Biotechnology and bioengineering

authors： Wiebe MG,Trinci AP

更新日期：1991-06-05 00:00:00

abstract：:Recombinant Escherichia coli harboring the medium-chain-length (MCL) polyhydroxyalkanoate (PHA) synthase gene has been shown to accumulate MCL-PHAs from fatty acids when FadB is inactive. However, the enzymes in fadB mutant E. coli responsible for channeling the beta-oxidation intermediates to PHA biosynthesis have no...

journal_title：Biotechnology and bioengineering

authors： Park SJ,Yup Lee S

更新日期：2004-06-20 00:00:00

abstract：:Streamline Direct CST I is a new type of ion exchanger with multi-modal functional groups, specially designed for an expanded bed adsorption (EBA) process, which can capture directly the proteins from the high ionic strength feedstocks with a high binding capacity. In this study, an experimental study is carried out f...

journal_title：Biotechnology and bioengineering

authors： Li P,Xiu G,Mata VG,Grande CA,Rodrigues AE

更新日期：2006-08-20 00:00:00

abstract：:Phaeodactylum tricornutum is a widely studied diatom and has been proposed as a source of oil and polyunsaturated fatty acids (PUFA), particularly eicosapentaenoic acid (EPA). Recent studies indicate that lipid accumulation occurs under nutritional stress. Aim of this research was to determine how changes in nitrogen ...

journal_title：Biotechnology and bioengineering

authors： Rodolfi L,Biondi N,Guccione A,Bassi N,D'Ottavio M,Arganaraz G,Tredici MR

更新日期：2017-10-01 00:00:00

abstract：:Spores, although often considered metabolically inert, catalyze a variety of reactions. The use of spores instead of mycelium for bioconversions has several advantages. In this paper, we describe the difference in susceptibility of mycelium and spores against toxic substrates and products. A higher resistance of spore...

journal_title：Biotechnology and bioengineering

authors： Wolken WA,Tramper J,van der Werf MJ

更新日期：2002-12-20 00:00:00

abstract：:We have shown previously that a sequence-specific DNA-binding protein based on the Lac repressor protein can isolate pre-purified DNA efficiently from simple buffer solution but our attempts to purify plasmids directly from crude starting materials were disappointing with impractically low DNA yields. We have optimize...

journal_title：Biotechnology and bioengineering

authors： Darby RA,Forde GM,Slater NK,Hine AV

更新日期：2007-12-01 00:00:00