Effect of liquid hot water pretreatment severity on properties of hardwood lignin and enzymatic hydrolysis of cellulose.

abstract：:Engineering protein expression in vitro or in vivo is usually straightforward for single genes, but remains challenging for multiple genes because of the requirement of coordinated control. RNA and protein overexpression strategies often exploit T7 RNA polymerase and its natural TPhi Class I terminator. However, this ...

journal_title：Biotechnology and bioengineering

authors： Du L,Gao R,Forster AC

更新日期：2009-12-15 00:00:00

abstract：:MicroRNAs are small non-coding RNAs that play a critical role in post-transcriptional control of gene expression. Recent publications of genomic sequencing data from the Chinese Hamster (CGR) and Chinese hamster ovary (CHO) cells provide new tools for the discovery of novel miRNAs in this important production system. ...

journal_title：Biotechnology and bioengineering

authors： Diendorfer AB,Hackl M,Klanert G,Jadhav V,Reithofer M,Stiefel F,Hesse F,Grillari J,Borth N

更新日期：2015-07-01 00:00:00

abstract：:Biosensors with animal and microbial cells immobilized close to the tip of a membrane electrode have been developed for chemical and drug testing. Our experimental results show that biosensors can be used for drug screening and to provide useful information about various cell-chemical interactions. A computer aided an...

journal_title：Biotechnology and bioengineering

authors： Li XM,Liang BS,Wang HY

更新日期：1988-02-20 00:00:00

abstract：:Conventional wet chemistry methods to determine biomass composition are labor- and time-intensive and require larger amounts of biomass (300 mg) than is often available. To overcome these limitations and to support a high-throughput pretreatment and hydrolysis (HTPH) screening system, this article reports on the devel...

journal_title：Biotechnology and bioengineering

authors： DeMartini JD,Studer MH,Wyman CE

更新日期：2011-02-01 00:00:00

abstract：:In vivo mesenchymal stem cell (MSC) survival is relevant to therapeutic applications requiring engraftment and potentially to nonengraftment applications as well. MSCs are a mixture of progenitors at different stages of cellular aging, but the contribution of this heterogeneity to the survival of MSC implants is unkno...

journal_title：Biotechnology and bioengineering

authors： Madsen SD,Jones SH,Tucker HA,Giler MK,Muller DC,Discher CT,Russell KC,Dobek GL,Sammarco MC,Bunnell BA,O'Connor KC

更新日期：2020-01-01 00:00:00

abstract：:An experimental device was constructed to allow nearly simultaneous measurements to be made on temperature and gas composition at different depths in a solid-substrate fermentation bed. The time-dependent values of temperature, mol % O(2) and mol % CO(2) were measured at five positions in beds 6.35 cm (2.5 in.) deep. ...

journal_title：Biotechnology and bioengineering

authors： Rathbun BL,Shuler ML

更新日期：1983-04-01 00:00:00

abstract：:The thickness variability of biofilms of Pseudomonas aeruginosa, Klebsiella pneumoniae, and the binary population combination of these two species was quantified. The experimental method involved cryoembedding biofilms with a commercial tissue embedding agent, sectioning, and applying image analysis to construct thick...

journal_title：Biotechnology and bioengineering

authors： Murga R,Stewart PS,Daly D

更新日期：1995-03-20 00:00:00

abstract：:Enzymatic acrylation is a process of potentially strong interest to the chemical industry. Direct esterification involving acrylic acid is unfortunately rather slow, with inhibition phenomena appearing at high acid concentrations. In the present study the acrylation of 1-octanol catalyzed by immobilized Candida antarc...

journal_title：Biotechnology and bioengineering

authors： Nordblad M,Adlercreutz P

更新日期：2008-04-15 00:00:00

abstract：:Use of cellulase for denim washing is a standard eco-friendly technique to achieve desirable appearance and softness for cotton fabrics and denims. But enzymatic washing of denim till date involved acid cellulase (Trichoderma reesei) and neutral cellulase (Humicola isolens) the use of which has a drawback of backstain...

journal_title：Biotechnology and bioengineering

authors： Anish R,Rahman MS,Rao M

更新日期：2007-01-01 00:00:00

abstract：:Nitrous oxide (N2 O), a potent greenhouse gas, is reduced to N2 gas by N2 O-reducing bacteria (N2 ORB), a process which represents an N2 O sink in natural and engineered ecosystems. The N2 O sink activity by N2 ORB depends on temperature and O2 exposure, yet the specifics are not yet understood. This study explores th...

journal_title：Biotechnology and bioengineering

authors： Zhou Y,Suenaga T,Qi C,Riya S,Hosomi M,Terada A

更新日期：2020-12-11 00:00:00

abstract：:Tumor-targeted Salmonella VNP20009 preferentially replicate within tumor tissue and partially suppress tumor growth in murine tumor models. These Salmonella have the ability to locally induce apoptosis when they are in direct contact with cancer cells but they lack significant bystander killing, which may correlate wi...

journal_title：Biotechnology and bioengineering

authors： Quintero D,Carrafa J,Vincent L,Bermudes D

更新日期：2016-12-01 00:00:00

abstract：:Clostridium difficile has emerged as a major cause of infectious diarrhea in hospitalized patients, with increasing mortality rate and annual healthcare costs exceeding $3 billion. Since C. difficile infections are associated with the use of antibiotics, there is an urgent need to develop treatments that can inactivat...

journal_title：Biotechnology and bioengineering

authors： Mehta KK,Paskaleva EE,Wu X,Grover N,Mundra RV,Chen K,Zhang Y,Yang Z,Feng H,Dordick JS,Kane RS

更新日期：2016-12-01 00:00:00

abstract：:The production of soluble microbial products (SMP) in anaerobic systems was evaluated using chemostat reactors. Results from steady-state and tracer experiments with (14)C-glucose and (14)C-acetate showed that significant amounts of SMP were produced during the acidogenesis of glucose, but that SMP did not accumulate ...

journal_title：Biotechnology and bioengineering

authors： Noguera DR,Araki N,Rittmann BE

更新日期：1994-11-05 00:00:00

abstract：:Bifidobacterium animalis ATCC 27536 represents among bifidobacteria a host-model for cloning experiments. The segregational and structural stabilities of a family of cloning vectors with different molecular weights but sharing a common core were studied in continuous fermentation of the hosting B. animalis without sel...

journal_title：Biotechnology and bioengineering

authors： González Vara A,Rossi M,Altomare L,Eikmanns B,Matteuzzi D

更新日期：2003-10-20 00:00:00

abstract：:Application of plasmid DNA as pharmaceutical to be used in gene therapy and vaccination has been investigated intensively in recent years. To be able to provide sufficient material that is in accordance with quality of pharmaceutical grade it is mandatory to gain comprehensive process knowledge which is even requested...

journal_title：Biotechnology and bioengineering

authors： Bohle K,Ross A

更新日期：2011-09-01 00:00:00

abstract：:Molecular descriptors represent structural and physicochemical features of compounds. They have been extensively used for developing statistical models, such as quantitative structure activity relationship (QSAR) and artificial neural networks (NN), for computer prediction of the pharmacodynamic, pharmacokinetic, or t...

journal_title：Biotechnology and bioengineering

authors： Li ZR,Han LY,Xue Y,Yap CW,Li H,Jiang L,Chen YZ

更新日期：2007-06-01 00:00:00

abstract：:Malathion is one of the most widely used organophosphorus pesticides in the United States and developing countries. Herein, we enhanced the degradation rate of malathion starting with a phosphotriesterase PoOPHM2 while also considering thermostability. In the first step, iterative saturation mutagenesis at residues li...

journal_title：Biotechnology and bioengineering

authors： Luo XJ,Zhao J,Li CX,Bai YP,Reetz MT,Yu HL,Xu JH

更新日期：2016-11-01 00:00:00

abstract：:On-line NAD(P)H fluorescence and culture redox potential (CRP) measurements were utilized to investigate the role of Vitreoscilla hemoglobin (VHb) in perturbing oxygen metabolism of microaerobic Escherichia coli Batch cultures of a VHb-synthesizing E. coli strain and the iso-genic control under fully aerated condition...

journal_title：Biotechnology and bioengineering

authors： Tsai PS,Rao G,Bailey JE

更新日期：1995-08-05 00:00:00

abstract：:Experimental and theoretical studies are conducted to understand the dynamic behavior of a continuous-flow fixed-bed reactor in which an esterification is catalyzed by an immobilized enzyme in an organic solvent medium. The experimental system consists of a commercial immobilized lipase preparation known as Lipozyme a...

journal_title：Biotechnology and bioengineering

authors： Mensah P,Gainer JL,Carta G

更新日期：1998-11-20 00:00:00

abstract：:Gene transcription is regulated by transcription factors that can bind to specific regions on DNA. Antigene oligonucleotides (oligos) can bind to specific regions on DNA and form a triplex with the double-stranded DNA. The triplex can competitively inhibit the binding of transcription factors and, as a result, transcr...

journal_title：Biotechnology and bioengineering

authors： Cheng B,Fournier RL,Relue PA

更新日期：2000-11-20 00:00:00

abstract：:Phosphorus-31 nuclear magnetic resonance ((31)P NMR) was used to compare the anaerobic metabolism of glucose by suspended and gel-entrapped Saccharomyces bayanus cells. The fermentation of glucose was carried out in a reaction system with continuous circulation through the NMR sample tube. The intracellular pH and the...

journal_title：Biotechnology and bioengineering

authors： Taipa MA,Cabral JM,Santos H

更新日期：1993-03-15 00:00:00

abstract：:Innovation in monoclonal antibody (mAb) production continues to be driven by cell engineering strategies to increase yield and improve product quality. In a previous study, to investigate the effectiveness of transporter overexpression strategies, we prepared a taurine transporter-overexpressing host cell line (DXB11/...

journal_title：Biotechnology and bioengineering

authors： Tabuchi H,Sugiyama T

更新日期：2013-08-01 00:00:00

abstract：:Knowledge of the aqueous phase methane concentration is critical to understanding and controlling process kinetics in methanotrophic bioreactors. Unfortunately since no dissolved methane probe is commercially available, this data must be obtained off-line by the time-consuming gas-liquid partition method. In this stud...

journal_title：Biotechnology and bioengineering

authors： Yu Y,Ramsay JA,Ramsay BA

更新日期：2006-12-05 00:00:00

abstract：:10-Deacetylabaccatin III (10 DAB), an important precursor for paclitaxel semisynthesis, is enhanced in yew extracts using C10-deacetylase and C13-deacylase enzymes.(4) C10-deacetylase is an intracellular enzyme produced by the fermentation of a soil microorganism, Nocardioides luteus (SC 13912). During the fermentatio...

journal_title：Biotechnology and bioengineering

authors： Nanduri VB,Hanson RL,Laporte TL,Ko RY,Patel RN,Szarka LJ

更新日期：1995-12-05 00:00:00

abstract：:This article compares the processes for wheat straw lignocellulose fractionation by percolation, counter-current progressing batch percolation and batch reaction at low NaOH-loadings (3-6% of DM). The flow-through processes were found to improve delignification and subsequent enzymatic saccharification, reduce NaOH-co...

journal_title：Biotechnology and bioengineering

authors： Pihlajaniemi V,Sipponen MH,Pastinen O,Nyyssölä A,Laakso S

更新日期：2016-12-01 00:00:00

abstract：:The design of new generation bioprocessing plants is increasingly dependent on the design of process-compatible microorganisms. The latter, whether through genetic or physiological manipulations, can be greatly assisted by metabolic engineering. An emerging powerful tool in metabolic engineering research is computer-a...

journal_title：Biotechnology and bioengineering

authors： Dean JP,Dervakos GA

更新日期：1998-04-05 00:00:00

abstract：:The effects ensuing from the interaction between membrane-bound sarcosine dehydrogenase and the surrounding lipids as well as the effects of membrane fluidity were described in this study. A 25-fold activation was observed upon the reconstitution of the enzyme in bilayers of SUVs made of DMPC. The considerable decreas...

journal_title：Biotechnology and bioengineering

authors： Kheirolomoom A,Katoh S,Sada E,Yoshida K

更新日期：1991-04-15 00:00:00

abstract：:This work investigated the use of nuclear magnetic resonance (NMR) spectroscopy in combination with a mathematical model of an encapsulated cell system as a method for rapidly assessing the status of a pancreatic substitute. To validate this method, an in vitro experiment was performed in which the encapsulated cells ...

journal_title：Biotechnology and bioengineering

authors： Gross JD,Long RC Jr,Constantinidis I,Sambanis A

更新日期：2007-09-01 00:00:00

abstract：:Enzymatic hydrolysis of arabinoxylan is an important prerequisite for the utilization of hemicellulose for ethanol fermentation or for making the low calorie sweetener xylitol by catalytic hydrogenation of the generated xylose. This study focus on cloning and characterization of two industrial relevant beta-xylosidase...

journal_title：Biotechnology and bioengineering

authors： Rasmussen LE,Sørensen HR,Vind J,Viksø-Nielsen A

更新日期：2006-08-05 00:00:00

abstract：:Spinner-flask bioreactors have been used for the production of articular cartilage in vitro. The dynamic environment within bioreactors is known to significantly affect the growth and development of the tissue. The present research focuses on the experimental and numerical characterization of the flow field within a s...

journal_title：Biotechnology and bioengineering

authors： Sucosky P,Osorio DF,Brown JB,Neitzel GP

更新日期：2004-01-05 00:00:00