Survival of aging CD264+ and CD264- populations of human bone marrow mesenchymal stem cells is independent of colony-forming efficiency.


:In vivo mesenchymal stem cell (MSC) survival is relevant to therapeutic applications requiring engraftment and potentially to nonengraftment applications as well. MSCs are a mixture of progenitors at different stages of cellular aging, but the contribution of this heterogeneity to the survival of MSC implants is unknown. Here, we employ a biomarker of cellular aging, the decoy TRAIL receptor CD264, to compare the survival kinetics of two cell populations in human bone marrow MSC (hBM-MSC) cultures. Sorted CD264+ hBM-MSCs from two age-matched donors have elevated β-galactosidase activity, decreased differentiation potential and form in vitro colonies inefficiently relative to CD264- hBM-MSCs. Counterintuitive to their aging phenotype, CD264+ hBM-MSCs exhibited comparable survival to matched CD264- hBM-MSCs from the same culture during in vitro colony formation and in vivo when implanted ectopically in immunodeficient NIH III mice. In vitro and in vivo survival of these two cell populations were independent of colony-forming efficiency. These findings have ramifications for the preparation of hBM-MSC therapies given the prevalence of aging CD264+ cells in hBM-MSC cultures and the popularity of colony-forming efficiency as a quality control metric in preclinical and clinical studies with MSCs.


Biotechnol Bioeng


Madsen SD,Jones SH,Tucker HA,Giler MK,Muller DC,Discher CT,Russell KC,Dobek GL,Sammarco MC,Bunnell BA,O'Connor KC




Has Abstract


2020-01-01 00:00:00












  • Newly identified bacteriolytic enzymes that target a wide range of clinical isolates of Clostridium difficile.

    abstract::Clostridium difficile has emerged as a major cause of infectious diarrhea in hospitalized patients, with increasing mortality rate and annual healthcare costs exceeding $3 billion. Since C. difficile infections are associated with the use of antibiotics, there is an urgent need to develop treatments that can inactivat...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Mehta KK,Paskaleva EE,Wu X,Grover N,Mundra RV,Chen K,Zhang Y,Yang Z,Feng H,Dordick JS,Kane RS

    更新日期:2016-12-01 00:00:00

  • H(2) photoproduction by batch culture of Anabaena variabilis ATCC 29413 and its mutant PK84 in a photobioreactor.

    abstract::Hydrogen production by Anabaena variabilis ATCC 29413 and of its mutant PK84, grown in batch cultures, was studied in a photobioreactor. The highest volumetric H(2) production rates of native and mutant strains were found in cultures grown at gradually increased irradiation. The native strain evolved H(2) only under a...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Tsygankov AA,Borodin VB,Rao KK,Hall DO

    更新日期:1999-09-20 00:00:00

  • Regulation of lac operon expression in mixed sugar chemostat cultures.

    abstract::Expression of the lactose (lac) operon in the Escherichia coli chromosome has been studied in mixed-sugar chemostat cultures under steady-state and transient conditions. A unified model has been formulated which involves regulation of active inducer (lactose) transport, promoter-operator regulated expression of the la...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ray NG,Vieth WR,Venkatasubramanian K

    更新日期:1987-06-01 00:00:00

  • Covalent dimerization of camelidae anti-human TNF-alpha single domain antibodies by the constant kappa light chain domain improves neutralizing activity.

    abstract::The tumor necrosis factor-alpha (TNFalpha) plays an important role in a number of chronic inflammatory disorders. Monoclonal camelidae variable heavy chain domain-only antibodies (V(H)H) have been developed to antagonize the action of human TNFalpha (anti-TNF-V(H)H). Here we describe a strategy to obtain functional di...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Giersberg M,Floss DM,Kipriyanov S,Conrad U,Scheller J

    更新日期:2010-05-01 00:00:00

  • Combinatorial evolution of phosphotriesterase toward a robust malathion degrader by hierarchical iteration mutagenesis.

    abstract::Malathion is one of the most widely used organophosphorus pesticides in the United States and developing countries. Herein, we enhanced the degradation rate of malathion starting with a phosphotriesterase PoOPHM2 while also considering thermostability. In the first step, iterative saturation mutagenesis at residues li...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Luo XJ,Zhao J,Li CX,Bai YP,Reetz MT,Yu HL,Xu JH

    更新日期:2016-11-01 00:00:00

  • A mechanistic model of the aerobic growth of Saccharomyces cerevisiae.

    abstract::A two-stage deterministic model of the growth of Saccharomyces cerevisiae is presented. The cell cycle of this organism was used to suggest the basic model structure. The model represents the preparatory processes of substrate uptake and conversion separately from replication and division. The regulation of the fracti...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Bijkerk AH,Hall RJ

    更新日期:1977-02-01 00:00:00

  • Kinetic and thermal characteristics of enzyme-graft copolymers.

    abstract::The characteristics of horseradish peroxidase (HRP) immobilized onto Sepharose by a photochemical-initiated graft copolymerization are presented. Active copolymers were synthesized using different amounts of glycidylmethacrylate (GMA), bisacryloylpiperazine (BAP), or 1,3,5-hexhydrotriacryloyl-(s)-triazine (HTsT) as fu...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Cremonesi P,D'Angiuro L

    更新日期:1983-03-01 00:00:00

  • Removal and upgrading of lignocellulosic fermentation inhibitors by in situ biocatalysis and liquid-liquid extraction.

    abstract::Hydroxycinnamic acids are known to inhibit microbial growth during fermentation of lignocellulosic biomass hydrolysates, and the ability to diminish hydroxycinnamic acid toxicity would allow for more effective biological conversion of biomass to fuels and other value-added products. In this work, we provide a proof-of...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Tomek KJ,Saldarriaga CR,Velasquez FP,Liu T,Hodge DB,Whitehead TA

    更新日期:2015-03-01 00:00:00

  • Tuning biphenyl dioxygenase for extended substrate specificity.

    abstract::Highly substituted polychlorinated biphenyls (PCBs) are known to be very resistant to aerobic biodegradation, particularly the initial attack by biphenyl dioxygenase. Functional evolution of the substrate specificity of biphenyl dioxygenase was demonstrated by DNA shuffling and staggered extension process (StEP) of th...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Brühlmann F,Chen W

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  • Three-dimensional fibrous PLGA/HAp composite scaffold for BMP-2 delivery.

    abstract::A protein loaded three-dimensional scaffold can be used for protein delivery and bone tissue regeneration. The main objective of this project was to develop recombinant human bone morphogenetic protein-2 (rhBMP-2) loaded poly(D,L-lactide-co-glycolide)/hydroxylapatite (PLGA/HAp) composite fibrous scaffolds through a pr...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Nie H,Soh BW,Fu YC,Wang CH

    更新日期:2008-01-01 00:00:00

  • Halophilic archaea and their potential to generate renewable fuels and chemicals.

    abstract::Lignocellulosic biofuels and chemicals have great potential to reduce our dependence on fossil fuels and mitigate air pollution by cutting down on greenhouse gas emissions. Chemical, thermal, and enzymatic processes are used to release the sugars from the lignocellulosic biomass for conversion to biofuels. These proce...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章,评审


    authors: Kasirajan L,Maupin-Furlow JA

    更新日期:2020-11-26 00:00:00

  • Synthesis of polyketides from low cost substrates by the thermotolerant yeast Kluyveromyces marxianus.

    abstract::Kluyveromyces marxianus is a promising nonconventional yeast for biobased chemical production due to its rapid growth rate, high TCA cycle flux, and tolerance to low pH and high temperature. Unlike Saccharomyces cerevisiae, K. marxianus grows on low-cost substrates to cell densities that equal or surpass densities in ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: McTaggart TL,Bever D,Bassett S,Da Silva NA

    更新日期:2019-07-01 00:00:00

  • Production of S-adenosyl-L-methionine by Saccharomyces cerevisiae cells carrying a gene for ethionine resistance.

    abstract::A gene for ethionine resistance isolated from the yeast Saccharomyces cerevisiae DKD-5D-H conferred on the yeast cells resistance to seleno-L-methionine and capability to produce S-adenosyl-L-methionine in the cells. An enzymatic study of the L-methionine synthetic pathway of L-methionine proto- and auxotrophs and in ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Shiomi N,Fukuda H,Fukuda Y,Murata K,Kimura A

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  • Fiber alignment and coculture with fibroblasts improves the differentiated phenotype of murine embryonic stem cell-derived cardiomyocytes for cardiac tissue engineering.

    abstract::Embryonic stem cells (ESCs) are an important source of cardiomyocytes for regenerating injured myocardium. The successful use of ESC-derived cardiomyocytes in cardiac tissue engineering requires an understanding of the important scaffold properties and culture conditions to promote cell attachment, differentiation, or...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Parrag IC,Zandstra PW,Woodhouse KA

    更新日期:2012-03-01 00:00:00

  • Increased recombinant protein production in Escherichia coli strains with overexpressed water-forming NADH oxidase and a deleted ArcA regulatory protein.

    abstract::Glycolytic flux is increased and acetate production is reduced in Escherichia coli by the expression of heterologous NADH oxidase (NOX) from Streptococcus pneumoniae coupled with the deletion of the arcA gene, which encodes the ArcA regulatory protein. In this study, we examined the overproduction of a model recombina...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Vemuri GN,Eiteman MA,Altman E

    更新日期:2006-06-20 00:00:00

  • Efficiency of bovine liver catalase as a catalyst to cleave H2O2 added continually to buffer solutions.

    abstract::Empirical estimations of H2O2 concentration in a system containing bovine liver catalase and continually supplied with H2O2 were done to evaluate the efficiency of the enzyme to cleave H2O2. It was found that the continuous addition of H2O2 leads to the formation of steady-state concentrations of H2O2 in the medium. A...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ibrahim M,Schlegel HG

    更新日期:1980-09-01 00:00:00

  • Characteristics and role of dynamic membrane layer in anaerobic membrane bioreactors.

    abstract::A submerged anaerobic dynamic membrane bioreactor (AnDMBR) was operated for treatment of concentrated wastewater. The dynamic membrane (DM) or cake layer was characterized on its physicochemical and biological composition and the role of the DM layer in treatment and filtration performances was assessed. The results s...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ersahin ME,Tao Y,Ozgun H,Spanjers H,van Lier JB

    更新日期:2016-04-01 00:00:00

  • Chemical inhibition of nitrification in activated sludge.

    abstract::Conventional aerobic nitrification was adversely affected by single pulse inputs of six different classes of industrially relevant chemical toxins: an electrophilic solvent (1-chloro-2,4-dinitrobenzene, CDNB), a heavy metal (cadmium), a hydrophobic chemical (1-octanol), an uncoupling agent (2,4-dinitrophenol, DNP), al...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Kelly RT 2nd,Henriques ID,Love NG

    更新日期:2004-03-20 00:00:00

  • A combined cell-cycle and metabolic model for the growth of hybridoma cells in steady-state continuous culture.

    abstract::The Model presented in this work demonstrates the combination of cell-cycle model with a model describing the growth and conversion kinetics of hybridoma cells in a steady-state continuous culture. The cell-cycle model is based upon a population balance model as described by Cazzador et al. and assumes the existence o...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Martens DE,Sipkema EM,de Gooijer CD,Beuvery EC,Tramper J

    更新日期:1995-10-05 00:00:00

  • The effect of heating rate on Escherichia coli metabolism, physiological stress, transcriptional response, and production of temperature-induced recombinant protein: a scale-down study.

    abstract::At the laboratory scale, sudden step increases from 30 to 42 degrees C can be readily accomplished when expressing heterologous proteins in heat-inducible systems. However, for large scale-cultures only slow ramp-type increases in temperature are possible due to heat transfer limitations, where the heating rate decrea...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Caspeta L,Flores N,Pérez NO,Bolívar F,Ramírez OT

    更新日期:2009-02-01 00:00:00

  • High-level recombinant protein production in CHO cells using lentiviral vectors and the cumate gene-switch.

    abstract::Fast and efficient production of recombinant proteins for structural and functional studies is a crucial issue for research and for industry. To this end, we have developed an efficient system to generate in less than 2 months, starting from the cDNA, pools of CHO cells stably expressing high-level of recombinant prot...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Gaillet B,Gilbert R,Broussau S,Pilotte A,Malenfant F,Mullick A,Garnier A,Massie B

    更新日期:2010-06-01 00:00:00

  • Two-stage fermentation with bacteriophage lambda as an expression vector in Escherachia coli.

    abstract::The potential of bacteriophage lambda as an expression vector for a large scale production of cloned-gene proteins was evaluated in batch and continuous bioreactors using a temperature-sensitive mutant in the cl gene, which allows a simple manipulation of temperature as a means to control the phage in the lysogenic or...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Park TH,Seo JH,Lim HC

    更新日期:1991-02-20 00:00:00

  • General theory of determining intraparticle active immobilized enzyme distribution and rate parameters.

    abstract::A general theory is presented in this article for determining the intrinsic rate constants for the main reaction and deactivation reaction, the effective diffusivity of the substrate, and the active enzyme distribution within porous solid supports from deactivation study of a continuous stirred-basket reactor (CSBR). ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Hossain MM,Do DD

    更新日期:1989-03-01 00:00:00

  • Development of waxy cassava with different Biological and physico-chemical characteristics of starches for industrial applications.

    abstract::The quality of cassava starch, an important trait in cassava breeding programs, determines its applications in various industries. For example, development of waxy (having a low level of amylose) cassava is in demand. Amylose is synthesized by granule-bound starch synthase I (GBSSI) in plants, and therefore, down-regu...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Zhao SS,Dufour D,Sánchez T,Ceballos H,Zhang P

    更新日期:2011-08-01 00:00:00

  • Permeability of alginate microcapsules to secretory recombinant gene products.

    abstract::Non-autologous somatic gene therapy is an alternate approach to delivering recombinant gene products through implantation of a "universal" donor cell line engineered to produce a therapeutic gene product. The cells are immunologically isolated by enclosure in immunoprotective microcapsules fabricated from alginate-pol...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Awrey DE,Tse M,Hortelano G,Chang PL

    更新日期:1996-11-20 00:00:00

  • Regulation mechanisms in mixed and pure culture microbial fermentation.

    abstract::Mixed-culture fermentation is a key central process to enable next generation biofuels and biocommodity production due to economic and process advantages over application of pure cultures. However, a key limitation to the application of mixed-culture fermentation is predicting culture product response, related to meta...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章,评审


    authors: Hoelzle RD,Virdis B,Batstone DJ

    更新日期:2014-11-01 00:00:00

  • Immobilization of yeast cells containing beta-galactosidase.

    abstract::Cultural conditions optimum for beta-galactosidase production by Saccharomyces anamensis are pH 4.5, temperature 26 +/- 2 degrees C, and 30 h of incubation period. Addition of lactose at 24 h fermentation greatly increase the level of enzyme. Optimum pHl, temperature, pH stability, and thermostability of yeast beta-ga...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Banerjee M,Chakrabarty A,Majumdar SK

    更新日期:1982-08-01 00:00:00

  • Metabolic engineering of Escherichia coli for the enhanced production of l-tyrosine.

    abstract::Despite wide applications of l-tyrosine in the market, microbial overproduction of l-tyrosine has been a great challenge due to the complex gene regulations involved in its biosynthetic pathway. To this end, effects of knocking out tyrR on the l-tyrosine production were further explored during the strain development. ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Kim B,Binkley R,Kim HU,Lee SY

    更新日期:2018-10-01 00:00:00

  • Simultaneous detection of three sex steroid hormone classes using a novel yeast-based biosensor.

    abstract::A biosensor detecting estrogens, progestogens, and androgens in complex samples and in a single step is described. Three Arxula adeninivorans yeast strains were created, each strain producing a different recombinant human hormone receptor and a different fluorescent reporter protein. These strains were then mixed to c...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Chamas A,Pham HTM,Jähne M,Hettwer K,Uhlig S,Simon K,Einspanier A,Baronian K,Kunze G

    更新日期:2017-07-01 00:00:00

  • Single-step unimolecular non-first-order enzyme deactivation kinetics.

    abstract::A two-parameter deactivation model is proposed to describe the kinetics of activity stabilization for some enzymes. The single-step unimolecular mechanism exhibits non-first-order deactivation kinetics since the final enzyme state, E(1) is not completely inactivated. The usefulness of the model is demonstrated by appl...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Sadana A,Henley JP

    更新日期:1987-10-20 00:00:00