Abstract:
:Non-destructive label-free continuous monitoring of in vitro tissue culture is an unmet demand in tissue engineering. Noting that different compositions of cartilage lead to different electrical tissue properties, we propose a new method to measure the electrical conductivity and its anisotropy during in vitro chondrogenesis. We used a conductivity tensor probe with 17 electrodes and a bio-impedance spectroscopy (BIS) device to measure the conductivity values and the anisotropy ratios at the bottom and top surfaces of the tissue samples during the culture period of 6 weeks. Clearly distinguishing glycosaminoglycans (GAGs), collagen, and also various mixtures of them, the measured conductivity value and the estimated tissue anisotropy provide diagnostic information of the depth-dependent tissue structure and compositions. Continuously monitoring the individual tissue during the entire chondrogenesis process without any adverse effect, the proposed method may significantly increase the productivity of cartilage tissue engineering.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Oh TI,Kim C,Karki B,Son Y,Lee E,Woo EJdoi
10.1002/bit.25350subject
Has Abstractpub_date
2015-02-01 00:00:00pages
422-7issue
2eissn
0006-3592issn
1097-0290journal_volume
112pub_type
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