Diversity matters: dynamic simulation of distributed bacterial states in suspended growth biological wastewater treatment systems.

abstract：:A dynamic method is proposed for k(l)a measurement in aerated and agitated reactors, in which a change in the total pressure in the reactor by approximately 20% leads to a simultaneous change in the oxygen concentration in all the bubbles in the dispersion. This procedure suppresses the influence of nonideal mixing of...

journal_title：Biotechnology and bioengineering

authors： Linek V,Benes P,Vacek V

更新日期：1989-05-01 00:00:00

abstract：:The treatment of meta- and para-nitrobenzoic acid in an industrial wastestream by Comamonas sp. JS46 and Comamonas sp. JS47 is investigated. The most important feature of the wastestream is the constantly changing concentration ratio of the two isomers. The most extreme occurrence is considered here: the complete chan...

journal_title：Biotechnology and bioengineering

authors： Goodall JL,Thomas SM,Spain JC,Peretti SW

更新日期：1998-07-05 00:00:00

abstract：:Glycosylation is a key critical quality attribute for monoclonal antibodies and other recombinant proteins because of its impact on effector mechanisms and half-life. In this study, a variety of compounds were evaluated for their ability to modulate glycosylation profiles of recombinant monoclonal antibodies produced ...

journal_title：Biotechnology and bioengineering

authors： Ehret J,Zimmermann M,Eichhorn T,Zimmer A

更新日期：2019-04-01 00:00:00

abstract：:Blood vessel development is critical for the continued growth and progression of solid tumors and, therefore, makes an attractive target for improving cancer therapy. Indeed, vascular-targeted therapies have been extensively explored but they have shown minimal efficacy as monotherapies. Combretastatin A4 (CA-4) is a ...

journal_title：Biotechnology and bioengineering

authors： Liu Y,Kim YJ,Siriwon N,Rohrs JA,Yu Z,Wanga P

更新日期：2018-06-01 00:00:00

abstract：:Pretreatment methods were compared with steam explosion, and differing views on the relative importance of mechanical and chemical effects were outlined. Hydrolysis was desirable; pyrolysis was undesirable. The effects of initial moisture content on steam consumption, mechanism and rate of heat transfer, pentosan solu...

journal_title：Biotechnology and bioengineering

authors： Brownell HH,Saddler JN

更新日期：1987-02-01 00:00:00

abstract：:RNA viruses are capable of undergoing extremely rapid evolution due to their high rates of reproduction, small genome size, and a high frequency of spontaneous mutagenesis. Here we demonstrate that a virus-like, evolutionary state can be created by propagating a phagemid population in a hypermutator strain of Escheric...

journal_title：Biotechnology and bioengineering

authors： Long-McGie J,Liu AD,Schellenberger V

更新日期：2000-04-05 00:00:00

abstract：:The development of a lentiviral system to deliver genes to specific cell types could improve the safety and the efficacy of gene delivery. Previously, we have developed an efficient method to target lentivectors to specific cells via an antibody-antigen interaction in vitro and in vivo. We report herein a targeted len...

journal_title：Biotechnology and bioengineering

authors： Froelich S,Ziegler L,Stroup K,Wang P

更新日期：2009-09-01 00:00:00

abstract：:Water permeability (Lp), calculated from the volume variations of cells subjected to an osmotic shock, is classically used to characterize cell membrane properties. In this work, we have shown the importance of the kind of mixing reactor used to measure the Lp parameter. A mathematical model including the mixing time ...

journal_title：Biotechnology and bioengineering

authors： de Marañón IM,Gervais P,Molin P

更新日期：1997-10-05 00:00:00

abstract：:To build complex genetic networks with predictable behaviors, synthetic biologists use libraries of modular parts that can be characterized in isolation and assembled together to create programmable higher-order functions. Characterization experiments and computational models for gene regulatory parts operating in iso...

journal_title：Biotechnology and bioengineering

authors： Westbrook A,Tang X,Marshall R,Maxwell CS,Chappell J,Agrawal DK,Dunlop MJ,Noireaux V,Beisel CL,Lucks J,Franco E

更新日期：2019-05-01 00:00:00

abstract：:Clostridium formicoaceticum homofermentatively converts lactate to acetate at 37 degrees C and pH 6.6-9.6. However, this fermentation is strongly inhibited by acetic acid at acidic pH. The specific growth rate of this organism decreased from a maximum at pH 7.6 to zero at pH 6.6. This inhibition effect was found to be...

journal_title：Biotechnology and bioengineering

authors： Tang IC,Okos MR,Yang ST

更新日期：1989-10-20 00:00:00

abstract：:A new high throughput technique for enzyme characterization with specific attention to the long term stability, called "Enzyme Test Bench," is presented. The concept of the Enzyme Test Bench consists of short term enzyme tests in 96-well microtiter plates under partly extreme conditions to predict the enzyme long term...

journal_title：Biotechnology and bioengineering

authors： Rachinskiy K,Schultze H,Boy M,Bornscheuer U,Büchs J

更新日期：2009-06-01 00:00:00

abstract：:Anaerobic digestion of biological organic particulates to methane has been described by a structured mathematical model based on multiple-reaction stoichiometry, conventional material balances, and liquid phase equilibrium chemistry. A general stoichiometric treatment for any set of multiple biological reactions is de...

journal_title：Biotechnology and bioengineering

authors： Bryers JD

更新日期：1985-05-01 00:00:00

abstract：:A mathematical model is formulated to describe trends in biomass and penicillin formation as well as substrate consumption for fed-batch cultivations. The biomass is structured into three morphological compartments, and glucose and corn steep liquor are considered as substrates for growth. Penicillin formation is assu...

journal_title：Biotechnology and bioengineering

authors： Zangirolami TC,Johansen CL,Nielsen J,Jørgensen SB

更新日期：1997-12-20 00:00:00

abstract：:The suitability of using annually grown, carrot-sized buffalo gourd (Cucurbita foetidissima) roots as a feedstock for alcoholic fermentation was explored. Roots grown in 1982 and 1983 were slurried, dextrinized and saccharified using Takathermtrade mark and Diazymetrade mark (commercial enzymes manufactured by Miles L...

journal_title：Biotechnology and bioengineering

authors： Scheerens JC,Kopplin MJ,Abbas IR,Nelson JM,Gathman AC,Berry JW

更新日期：1987-03-01 00:00:00

abstract：:Acetate accumulation is a common problem observed in aerobic high cell density Escherichia coli cultures. A previous report has hypothesized that the glyoxylate shunt is active in a low acetate producer, E. coli BL21, and inactive in a high acetate producer, JM109. To further investigate this hypothesis, we now develo...

journal_title：Biotechnology and bioengineering

authors： Noronha SB,Yeh HJ,Spande TF,Shiloach J

更新日期：2000-05-05 00:00:00

abstract：:The anaerobic oxidation of ammonium (anammox) uses nitrite as terminal electron acceptor. The nitrite can cause inhibition to the bacteria that catalyze the anammox reaction. The literature shows a great divergence on the levels of NO2 (-) causing inhibition. Moreover, the conditions influencing the resistance of anam...

journal_title：Biotechnology and bioengineering

authors： Carvajal-Arroyo JM,Puyol D,Li G,Sierra-Álvarez R,Field JA

更新日期：2014-10-01 00:00:00

abstract：:Connexin-43 (Cx43) containing giant liposomes (GL) were prepared by a baculovirus expression-liposome fusion method. Recombinant budded viruses expressing Cx43 were prepared and then fused with GLs containing DOPG/DOPC at pH 4.5. Connexon formation on the GL membrane was observed by transmission electron microscope. H...

journal_title：Biotechnology and bioengineering

authors： Kamiya K,Tsumoto K,Arakawa S,Shimizu S,Morita I,Yoshimura T,Akiyoshi K

更新日期：2010-12-01 00:00:00

abstract：:Protein phosphorylation is an important post-translational modification for intracellular signaling molecules, mostly found in serine and threonine residues. Tyrosine phosphorylations are very few events (less than 0.1% to phosphorylated serine/threonine residues), but capable of governing cell fate decisions involved...

journal_title：Biotechnology and bioengineering

authors： Yoshimoto N,Ikeda Y,Tatematsu K,Iijima M,Nakai T,Okajima T,Tanizawa K,Kuroda S

更新日期：2016-08-01 00:00:00

abstract：:The United States produces more than 10 million tons of waste oils and fats each year. This paper aims to establish a new biomanufacturing platform that converts waste oils or fats into a series of value-added products. Our research employs the oleaginous yeast Yarrowia lipolytica as a case study for citric acid (CA) ...

journal_title：Biotechnology and bioengineering

authors： Liu N,Soong YV,Mirzaee I,Olsen A,Yu P,Wong HW,Xie D

更新日期：2021-01-20 00:00:00

abstract：:After injury to the central nervous system, a glial scar develops that physically and biochemically inhibits axon growth. In the scar, activated astrocytes secrete inhibitory extracellular matrix, of which chondroitin sulfate proteoglycans (CSPGs) are considered the major inhibitory component. An inhibitory interface ...

journal_title：Biotechnology and bioengineering

authors： Tuinstra HM,Ducommun MM,Briley WE,Shea LD

更新日期：2013-03-01 00:00:00

abstract：:DNA/polyethylenimine (PEI) polyplexes are an important class of nonviral vectors. Although the conventional preparation method, bulk mixing, is straightforward, the formation of the DNA/PEI polyplexes is not well controlled. This work explores coaxial electrohydrodynamic spraying (EHDS) as a novel, alternative method ...

journal_title：Biotechnology and bioengineering

authors： Wu Y,Fei Z,Lee LJ,Wyslouzil BE

更新日期：2010-03-01 00:00:00

abstract：:The simultaneous biodegradation of the nonionic surfactant Tween 20 (Tw20) and pentachlorophenol (PCP) by Sphingomonas chlorophenolica sp. Strain RA2 (RA2) was measured. As a sole substrate, Tw20 biodegradation was best described by the Contois kinetic model. During concurrent biodegradation of Tw20 and PCP, the biode...

journal_title：Biotechnology and bioengineering

authors： Bielefeldt AR,Cort T

更新日期：2005-03-20 00:00:00

abstract：:Ethanol-producing bioreactors employing cells of Zymomonas mobilis attached to glass-fiber pads were operated continuously for as long as 28 days. Ethanol production, which is related to bed-associated biomass levels, was found to occur in three distinct phases: an exponential phase, a linear phase, and a "steady-stat...

journal_title：Biotechnology and bioengineering

authors： Arcuri EJ

更新日期：1982-03-01 00:00:00

abstract：:With a view toward reduction of graft loss, we explored pancreatic islet transplantation within fibrin matrices rendered pro-angiogenic by incorporation of minimal doses of vascular endothelial growth factor-A165 and platelet-derived growth factor-BB presented complexed to a fibrin-bound integrin-binding fibronectin d...

journal_title：Biotechnology and bioengineering

authors： Najjar M,Manzoli V,Abreu M,Villa C,Martino MM,Molano RD,Torrente Y,Pileggi A,Inverardi L,Ricordi C,Hubbell JA,Tomei AA

更新日期：2015-09-01 00:00:00

abstract：:Proteinaceous organic wastes are suitable substrates to produce high added-value products in anaerobic mixed-culture fermentations. In these processes, the stoichiometry of the biotransformation depends highly on operational conditions such as pH or feeding characteristics and there are still no tools that allow the p...

journal_title：Biotechnology and bioengineering

authors： Regueira A,Lema JM,Carballa M,Mauricio-Iglesias M

更新日期：2020-01-01 00:00:00

abstract：:The emergence of medicinal indications for stem cell therapies has seen a need to develop the manufacturing capacity for adherent cells such as mesenchymal stem cells (MSCs). One such development is in the use of microcarriers, which facilitate enhanced cell densities for adherent stem cell cultures when compared with...

journal_title：Biotechnology and bioengineering

authors： Odeleye AOO,Castillo-Avila S,Boon M,Martin H,Coopman K

更新日期：2017-09-01 00:00:00

abstract：:D-Methionine was converted to L-methionine in a reaction system where four enzymes were used. D-amino acid oxidase (D-AAO) from Arthrobacter protophormiae was used for the complete conversion of D-methionine to 2-oxo-4-methylthiobutyric acid. Catalase was added to prevent 2-oxo-4-methylthiobutyric acid decarboxylation...

journal_title：Biotechnology and bioengineering

authors： Findrik Z,Vasić-Racki D

更新日期：2007-12-01 00:00:00

abstract：:Cell culture longevity in fed-batch culture of hybridomas is often limited by elevated medium osmolality caused by repeated nutrient feeding. Shotwise feeding of 10x Dulbecco's modified Eagle's medium (DMEM) concentrates elevated the osmolality of medium up to 540 mOsm/kg at the end of fed-batch culture of S3H5/gamma2...

journal_title：Biotechnology and bioengineering

authors： Ryu JS,Lee GM

更新日期：1999-01-05 00:00:00

abstract：:Enterokinase and recombinant enterokinase light chain (rEK(L)) have been used widely to cleave fusion proteins with the target sequence of (Asp)(4)-Lys. In this work, we show that their utility as a site-specific cleavage agent is compromised by sporadic cleavage at other sites, albeit at low levels. Further degradati...

journal_title：Biotechnology and bioengineering

authors： Choi SI,Song HW,Moon JW,Seong BL

更新日期：2001-12-20 00:00:00

abstract：:An extractive acetonobutylic fermentation process is developed by integrating bioproduction, Ultrafiltration, and distillation, providing simultaneous retention of biomass, selective removal of inhibitors from the permeate, as well as separation and purification of acetone-butanol-ethanol solvents. Successive batch fe...

journal_title：Biotechnology and bioengineering

authors： Minier M,Grateloup R,Blanc-Ferras E,Goma G

更新日期：1990-04-15 00:00:00