Targeted gene delivery to CD117-expressing cells in vivo with lentiviral vectors co-displaying stem cell factor and a fusogenic molecule.

abstract：:This research investigates the effect of solids retention time (SRT) on the acid-phase anaerobic digestion of primary sludge. A series of experiments were conducted using two continuous-flow 3-L units with the following configuration: a completely mixed reactor (CMR) with clarifier and solids recycle and an upflow ana...

journal_title：Biotechnology and bioengineering

authors： Elefsiniotis P,Oldham WK

更新日期：1994-06-05 00:00:00

abstract：:The diffusion of viruses toward cells is a limiting step of the infection process. To be modeled correctly, this step must be evaluated in combination with the adsorption of the virus to the cell surface, which is a rapid but reversible step. In this paper, the recombinant adenovirus (rAd) diffusion and its adsorption...

journal_title：Biotechnology and bioengineering

authors： Gilbert PA,Kamen A,Bernier A,Garnier A

更新日期：2007-09-01 00:00:00

abstract：:Recombinant cell growth and protein synthesis by a recombinant Escherichia coli under various inducing conditions are compared to the predictions of a mathematical model. The mathematical model used was a combination of two literature models: (1) an empirical kinetic model for recombinant growth and product formation ...

journal_title：Biotechnology and bioengineering

authors： Betenbaugh MJ,Dhurjati P

更新日期：1990-06-20 00:00:00

abstract：:A model for cometabolism is verified experimentally for a defined methanotrophic mixed culture. The model includes the effects of cell growth, endogenous cell decay, product toxicity, and competitive inhibition with the assumption that cometabolic transformation rates are enhanced by reducing power obtained from oxida...

journal_title：Biotechnology and bioengineering

authors： Chang WK,Criddle CS

更新日期：1997-12-05 00:00:00

abstract：:Predicting and improving oxygen transport within bioartificial liver (BAL) devices continues to be an important engineering challenge since oxygen is one of the critical nutrients necessary for maintaining hepatocyte viability and function. Such a computational model would not only help predict outcomes but it would a...

journal_title：Biotechnology and bioengineering

authors： McClelland RE,MacDonald JM,Coger RN

更新日期：2003-04-05 00:00:00

abstract：:Tumor-targeted Salmonella VNP20009 preferentially replicate within tumor tissue and partially suppress tumor growth in murine tumor models. These Salmonella have the ability to locally induce apoptosis when they are in direct contact with cancer cells but they lack significant bystander killing, which may correlate wi...

journal_title：Biotechnology and bioengineering

authors： Quintero D,Carrafa J,Vincent L,Bermudes D

更新日期：2016-12-01 00:00:00

abstract：:Acidic Golgi pH plays an important role in protein glycosylation, one of the critical quality attributes of therapeutic proteins. To determine the intracellular Golgi pH during culture, stable Chinese hamster ovary (CHO) cell clones expressing pHluorin2, a ratiometric pH-sensitive fluorescent protein (FP), in the cis-...

journal_title：Biotechnology and bioengineering

authors： Lee JH,Kim J,Park JH,Heo WD,Lee GM

更新日期：2019-05-01 00:00:00

abstract：:A framework using material balances in metabolic pathways to study cellular metabolism is examined and the results are discussed. Rate measurements on extracellular compounds alone were found to be not always sufficient to validate proposed unique intracellular mechanisms. The conditions to delineate among candidate m...

journal_title：Biotechnology and bioengineering

authors： Niranjan SC,San KY

更新日期：1989-08-05 00:00:00

abstract：:The anaerobic digestion of a cellulose-enriched fraction of domestic refuse by means of rumen microorganisms in an "artificial rumen" digester was studied. Various combinations of solid and liquid retention times and loading rates were applied to establish optimum conditions for the acidogenic phase digestion of the r...

journal_title：Biotechnology and bioengineering

authors： Gijzen HJ,Zwart KB,Teunissen MJ,Vogels GD

更新日期：1988-09-05 00:00:00

abstract：:There are a series of examples in which phenols appear as contaminants in process streams and their selective removal is required for waste minimization. For the selective removal of a phenol from a mixture, we are exploiting the substrate specificity of the enzyme tyrosinase to convert phenols into reactive o-quinone...

journal_title：Biotechnology and bioengineering

authors： Sun WQ,Payne GF

更新日期：1996-07-05 00:00:00

abstract：:Utilising whole cells of recombinant Escherichia coli K27 (pCYP102, pGEc47) containing active cytochrome P450BM-3 monooxygenase [E.C. 1. 14.14.1], multiple oxidations of saturated and unsaturated fatty acids were performed by the enzyme under conditions of excess oxygen. The amount of oxygen dissolved in the culture m...

journal_title：Biotechnology and bioengineering

authors： Schneider S,Wubbolts MG,Oesterhelt G,Sanglard D,Witholt B

更新日期：1999-08-05 00:00:00

abstract：:A whole cell-based optical sensing system for copper was developed based on Saccharomyces cerevisiae cells harboring plasmid pYEX-GFPuv. The basis of this system was the ability of the transcriptional activator protein Ace1 present in S. cerevisiae to control the expression of the reporter protein, GFPuv. When copper ...

journal_title：Biotechnology and bioengineering

authors： Shetty RS,Deo SK,Liu Y,Daunert S

更新日期：2004-12-05 00:00:00

abstract：:Thermodynamic feasibility analysis (TFA) has been used as a tool capable of providing additional constraints to the mass balance-based methods of analysis of metabolic networks (e.g., flux balance analysis). Several publications have recently appeared in which TFA of different metabolic pathways from relatively simple...

journal_title：Biotechnology and bioengineering

authors： Vojinović V,von Stockar U

更新日期：2009-07-01 00:00:00

abstract：:The study reports on the development of a bioreactor for the production of alpha-keto acids from D,L- or D-amino acids using Rhodotorula gracilis D-amino acid oxidase. D-amino acid oxidase was co-immobilized with catalase on Affi-Gel 10 matrix, and the reactor was operated as a continuous-stirred tank reactor (CSTR) o...

journal_title：Biotechnology and bioengineering

authors： Butò S,Pollegioni L,D'Angiuro L,Pilone MS

更新日期：1994-12-01 00:00:00

abstract：:In this study, the effects of the electric charges and fields on the viability of airborne microorganisms were investigated. The electric charges of different magnitude and polarity were imparted on airborne microbial cells by a means of induction charging. The airborne microorganisms carrying different electric charg...

journal_title：Biotechnology and bioengineering

authors： Mainelis G,Górny RL,Reponen T,Trunov M,Grinshpun SA,Baron P,Yadav J,Willeke K

更新日期：2002-07-20 00:00:00

abstract：:Uranium competitive uptake experiments by Rhizopus arrhizus were carried out at three different solution pH levels and in the presence of different concentrations of competing aluminum ions in order to examine the competing ion effect. The coion effect became more pronounced as the coion concentration in solution and ...

journal_title：Biotechnology and bioengineering

authors： Tsezos M,Georgousis Z,Remoudaki E

更新日期：1997-07-05 00:00:00

abstract：:An aeration tank/sedimentation tank system is considered for large variations of organic loading. In this case, it is important to take into account the variability of the characteristics describing the oxidation of activated sludge as well as its sedimentation. The minimum cost of the treatment system is also evaluat...

journal_title：Biotechnology and bioengineering

authors： Vasiliev VB,Vavilin VA

更新日期：1982-11-01 00:00:00

abstract：:A new biomass productivity model was parameterized for Chlorella vulgaris using short-term (<30 min) oxygen productivities from algal microcosms exposed to 6 light intensities (20-420 W/m(2)) and 6 temperatures (5-42 °C). The model was then validated against experimental biomass productivities recorded in bench-scale ...

journal_title：Biotechnology and bioengineering

authors： Béchet Q,Chambonnière P,Shilton A,Guizard G,Guieysse B

更新日期：2015-05-01 00:00:00

abstract：:The effects ensuing from the interaction between membrane-bound sarcosine dehydrogenase and the surrounding lipids as well as the effects of membrane fluidity were described in this study. A 25-fold activation was observed upon the reconstitution of the enzyme in bilayers of SUVs made of DMPC. The considerable decreas...

journal_title：Biotechnology and bioengineering

authors： Kheirolomoom A,Katoh S,Sada E,Yoshida K

更新日期：1991-04-15 00:00:00

abstract：:Fed-batch culture of Bacillus thuringiensis in a modified airlift reactor has been developed by using adaptive control of glucose concentration in the reactor. The glucose concentration was estimated via a correlation equation between carbon dioxide production rate and glucose consumption rate. The estimated glucose c...

journal_title：Biotechnology and bioengineering

authors： Jong JZ,Hsiun DY,Wu WT,Tzeng YM

更新日期：1995-11-05 00:00:00

abstract：:Aggregates of Escherichia coli confined within hollowfiber reactors were either formed in place by culturing cells within the reactors, or were prepared by injecting a cell suspension that had been concentrated by centrifugation. The effective diffusive permeability of an uncharged nonreacting tracer, nitrous oxide, w...

journal_title：Biotechnology and bioengineering

authors： Libicki SB,Salmon PM,Robertson CR

更新日期：1988-06-20 00:00:00

abstract：:An automated system with semi on-line monitoring of glucose, lactic acid, protein, and optical density during lactic acid fermentations, is set up to study the dynamics of lactic acid bacteria. The analyzers for glucose, lactic acid, and protein are based on flow injection analysis (FIA). The system consists of a labo...

journal_title：Biotechnology and bioengineering

authors： Nielsen J,Nikolajsen K,Villadsen J

更新日期：1989-04-05 00:00:00

abstract：:The compound 5-hydroxymethylfurfural (HMF) has attracted much attention due to its versatility as an important bio-based platform chemical. Here, we engineered Raoultella ornithinolytica BF60 as a whole-cell biocatalyst for a highly efficient synthesis of 2,5-furandicarboxylic acid (FDCA) from HMF. Specifically, vario...

journal_title：Biotechnology and bioengineering

authors： Yuan H,Liu Y,Li J,Shin HD,Du G,Shi Z,Chen J,Liu L

更新日期：2018-09-01 00:00:00

abstract：:Ralstonia eutropha grows on and produces polyhydroxyalkanoates (PHAs) from fermentation acids. Acetic acid, one major organic acid from acidogenesis of organic wastes, has an inhibitory effect on the bacterium at slightly alkaline pH (6 g HAc/L at pH 8). The tolerance of R. eutropha to acetate, however, was increased ...

journal_title：Biotechnology and bioengineering

authors： Yu J,Wang J

更新日期：2001-06-20 00:00:00

abstract：:The quality of cassava starch, an important trait in cassava breeding programs, determines its applications in various industries. For example, development of waxy (having a low level of amylose) cassava is in demand. Amylose is synthesized by granule-bound starch synthase I (GBSSI) in plants, and therefore, down-regu...

journal_title：Biotechnology and bioengineering

authors： Zhao SS,Dufour D,Sánchez T,Ceballos H,Zhang P

更新日期：2011-08-01 00:00:00

abstract：:A respiratory-deficient, mutant of Kluyveromyces fragilis was isolated using a ethidium bromide mutagenesis. It was characterized by a loss of cytochromes a + a3 and by an improvement of its inulinase activity. Under anaerobic conditions this mutant was always better than the wild strain for ethanol production especia...

journal_title：Biotechnology and bioengineering

authors： Guiraud JP,Bourgi J,Stervinou M,Claisse M,Galzy P

更新日期：1987-05-01 00:00:00

abstract：:The solution structure of plasmid DNA is known to be a strong function of solution conditions due to intramolecular electrostatic interactions between the charged phosphate groups along the DNA backbone. The objective of this work was to determine whether it was possible to enhance the use of ultrafiltration for separ...

journal_title：Biotechnology and bioengineering

authors： Li Y,Currie D,Zydney AL

更新日期：2016-04-01 00:00:00

abstract：:Application of the well-characterized Streptococcus pyogenes CRISPR-Cas9 system in actinomycetes streptomycetes has enabled high-efficiency multiplex genome editing and CRISPRi-mediated transcriptional regulation in these prolific bioactive metabolite producers. Nonetheless, SpCas9 has its limitations and can be ineff...

journal_title：Biotechnology and bioengineering

authors： Yeo WL,Heng E,Tan LL,Lim YW,Lim YH,Hoon S,Zhao H,Zhang MM,Wong FT

更新日期：2019-09-01 00:00:00

abstract：:Enzyme clustering into compact agglomerates could accelerate the processing of intermediates to enhance metabolic pathway flux. However, enzyme clustering is still a challenging task due to the lack of universal assembly strategy applicable to all enzymes. Therefore, we proposed an alternative enzyme assembly strategy...

journal_title：Biotechnology and bioengineering

authors： Lv X,Jin K,Wu Y,Zhang C,Cui S,Zhu X,Li J,Du G,Liu L

更新日期：2020-05-01 00:00:00

abstract：:The in vitro generation of cell-based three dimensional (3D) nerve tissue is an attractive subject to improve graft survival and integration into host tissue for neural tissue regeneration or to model biological events in stem cell differentiation. Although 3D organotypic culture strategies are well established for 3D...

journal_title：Biotechnology and bioengineering

authors： Kim BC,Jun SM,Kim SY,Kwon YD,Choe SC,Kim EC,Lee JH,Kim J,Suh JF,Hwang YS

更新日期：2017-04-01 00:00:00