Anaerobic digestion of cellulose fraction of domestic refuse by means of rumen microorganisms.

abstract：:The transesterification reaction of N-acetyl-L-phenylalanine ethyl ester with 1-propanol catalyzed by alpha-chymotrypsin was examined in the ionic liquids 1-butyl-3-methylimidazolium hexafluorophosphate ([bmim][PF(6)]) and 1-octyl-3-methylimidazolium hexafluorophosphate ([omim][PF(6)]), and in combination with supercr...

journal_title：Biotechnology and bioengineering

authors： Laszlo JA,Compton DL

更新日期：2001-10-20 00:00:00

abstract：:Cytidine-5'-diphosphocholine (CDP-choline) is a widely used neuroprotective drug for multiple indications. In industry, CDP-choline is synthesized by a two-step cell culture/permeabilized cell biotransformation method because substrates often do not enter cells in an efficient manner. This study develops a novel one-s...

journal_title：Biotechnology and bioengineering

authors： Ren Y,Liu Q,Liu H,Zhou X,Zhang Y,Cai M

更新日期：2020-05-01 00:00:00

abstract：:The effector functions elicited by IgG antibodies strongly depend on the carbohydrate moiety linked to the Fc region of the protein. Therefore several approaches have been developed to rationally manipulate these glycans and improve the biological functions of the antibody. Overexpression of recombinant beta1,4-N-acet...

journal_title：Biotechnology and bioengineering

authors： Ferrara C,Brünker P,Suter T,Moser S,Püntener U,Umaña P

更新日期：2006-04-05 00:00:00

abstract：:Fed-batch cultures of recombinant microorganisms have attracted attention as they can separate cell growth stage from cloned-gene expression phase during fermentations. In this work, the effect of different glucose feeding strategies on cell growth and cloned gene expression was studied during aerobic fed-batch fermen...

journal_title：Biotechnology and bioengineering

authors： Patkar A,Seo JH

更新日期：1992-06-05 00:00:00

abstract：:The effect of trehalose (0.5 M) on the thermal stability of cutinase in the alkaline pH range was studied. The thermal unfolding induced by increasing temperature was analyzed in the absence and in the presence of trehalose according to a two-state model (which assumes that only the folded and unfolded states of cutin...

journal_title：Biotechnology and bioengineering

authors： Baptista RP,Cabral JM,Melo EP

更新日期：2000-12-20 00:00:00

abstract：:Tissue engineering devices as in vitro cell culture systems in scaffolds has encountered the bottleneck due to their much lower cell functions than real tissues/organs in vivo. Such situation has been improved in some extent by mimicking the cell microenvironments in vivo from either chemical or physical ways. However...

journal_title：Biotechnology and bioengineering

authors： Shen C,Meng Q,Zhang G

更新日期：2013-08-01 00:00:00

abstract：:The performance of an industrial pharmaceutical process (production of an active pharmaceutical ingredient by fermentation, API) was modeled by multiblock partial least squares (MBPLS). The most important process stages are inoculum production and API production fermentation. Thirty batches (runs) were produced accord...

journal_title：Biotechnology and bioengineering

authors： Lopes JA,Menezes JC,Westerhuis JA,Smilde AK

更新日期：2002-11-20 00:00:00

abstract：:Hyperacute rejection following xenogeneic transplantation in primates is mediated by naturally occurring IgM antibodies, which are specifically directed to alpha-Galactosyl residues on many nonprimate mammalian cells. Current approaches to remove these anti-alphaGal IgM include plasmapheresis followed by immunoaffinit...

journal_title：Biotechnology and bioengineering

authors： Karoor S,Molina J,Buchmann CR,Colton C,Logan JS,Henderson LW

更新日期：2003-01-20 00:00:00

abstract：:Although one of the major factors limiting the application of Bacillus subtilis as an expression host has been its production of at least eight extracellular proteases, researchers have also noticed that some proteases benefited the secretion of foreign proteins at times. Therefore, to maximize the yield of a foreign ...

journal_title：Biotechnology and bioengineering

authors： Zhao L,Ye B,Zhang Q,Cheng D,Zhou C,Cheng S,Yan X

更新日期：2019-08-01 00:00:00

abstract：:A specially designed model reactor based on a 42-L laboratory fermentor was equipped with six stirrers (Rushton turbines) and five cylindrical disks. In this model reactor, the mixing time, Theta(90), turned out to be 13 times longer compared with the 42-L standard laboratory fermentor fitted with two Rushton turbines...

journal_title：Biotechnology and bioengineering

authors： Schilling BM,Pfefferle W,Bachmann B,Leuchtenberger W,Deckwer W

更新日期：1999-09-05 00:00:00

abstract：:A mathematical model of glycolysis in Saccharomyces cerevisiae is presented. The model is based on rate equations for the individual reactions and aims to predict changes in the levels of intra- and extracellular metabolites after a glucose pulse, as described in part I of this study. Kinetic analysis focuses on a tim...

journal_title：Biotechnology and bioengineering

authors： Rizzi M,Baltes M,Theobald U,Reuss M

更新日期：1997-08-20 00:00:00

abstract：:The relationship between local mass transfer coefficient and fluid velocity in heterogenous biofilms was investigated by combining microelectrodes and confocal scanning laser microscopy (CSLM). The biofilms were grown for up to 7 days and consisted of cell clusters separated by interstitial channels. Mass transfer coe...

journal_title：Biotechnology and bioengineering

authors： Stoodley P,Yang S,Lappin-Scott H,Lewandowski Z

更新日期：1997-12-20 00:00:00

abstract：:To alleviate plasmid instability and to prolong the production phase of subtilisin, integrable plasmid and spore mutants are used. Compared with batch-type shake flask cultures, spore mutants' ability to produce subtilisin can be well pronounced in fed-batch and continuous cultures. Hence, the two culture methods make...

journal_title：Biotechnology and bioengineering

authors： Oh MK,Kim BG,Park SH

更新日期：1995-09-20 00:00:00

abstract：:It is shown that the mass transfer resistance can significantly distort the linearity of the Lineweaver-Burk plot of the kinetic data for a microbial culture which forms aggregates. For small flocs, the linearity of the Lineweaver-Burk plot is largely retained, but a different slope and intercept will be obtained comp...

journal_title：Biotechnology and bioengineering

authors： Ngian KF,Lin SH,Martin WR

更新日期：1977-12-01 00:00:00

abstract：:We developed a novel method to load and unload molecular cargos to and from microtubules (MTs) that move on kinesin-coated surfaces. Quantum dots (Qds) (molecular cargo) connected to 21-mer DNA can be selectively loaded on DNA-conjugated MTs through DNA hybridization. The average velocity of the Qd-loaded MTs (0.43 +/...

journal_title：Biotechnology and bioengineering

authors： Taira S,Du YZ,Hiratsuka Y,Uyeda TQ,Yumoto N,Kodaka M

更新日期：2008-02-15 00:00:00

abstract：:Aspergillus awamori NRRL 4869 was cultured on the solid substrate, wheat bran, in a modified Rollacell apparatus to produce alpha-galactosidase and invertase. The swivel cap on the elongated bottle permits the introduction of air while the bottle rotates. Parameters of air flow rate (0.05-0.2 liter/kg/min), rpm (0.15-...

journal_title：Biotechnology and bioengineering

authors： Silman RW

更新日期：1980-02-01 00:00:00

abstract：:It has long been established that UVC light is a very effective method for inactivating pathogens in a fluid, yet the application of UVC irradiation to modern biotechnological processes is limited by the intrinsic short penetration distance of UVC light in optically dense protein solutions. This experimental and numer...

journal_title：Biotechnology and bioengineering

authors： Ren Y,Crump CM,Mackley MM,Li Puma G,Reis NM

更新日期：2016-07-01 00:00:00

abstract：:The scope in improving enzyme productivities from the cellulose fermentation process is examined in laboratory-scale fermentors. The maximum productivity (30 IU/liter hr) is attained in a continuous-culture process with cell recycle using modified medium containing 0.5% cellulose. Optimum dilution rate and recycle rat...

journal_title：Biotechnology and bioengineering

authors： Ghose TK,Sahai V

更新日期：1979-02-01 00:00:00

abstract：:Monolith reactors combine good mass transfer characteristics with low-pressure drop, the principle factors affecting the cost effectiveness of industrial processes. Recently, these specific features of the monolith reactors have drawn the attention toward the application of the monolith reactor in multiphase reaction ...

journal_title：Biotechnology and bioengineering

authors： Ebrahimi S,Kleerebezem R,Kreutzer MT,Kapteijn F,Moulijn JA,Heijnen JJ,van Loosdrecht MC

更新日期：2006-02-05 00:00:00

abstract：:Melanoma is now the fifth most common type of cancer in North America. At present, there is no optimal treatment for this cancer. However, the lowering of the tyrosine level can inhibit the growth of melanoma. Unfortunately, this diet restriction cannot be humanly tolerated and causes vomiting, nausea, and severe body...

journal_title：Biotechnology and bioengineering

authors： Yu B,Chang TM

更新日期：2004-06-30 00:00:00

abstract：:Relative levels of many individual proteins in Escherichia coli HB101 strains with 0, 37, 56, and 240 plasmids per chromosome were determined by computer image analysis of two-dimensional gel electrophoresis patterns. The plasmids investigated had very similar sequences except for small domains encoding the repressor ...

journal_title：Biotechnology and bioengineering

authors： Birnbaum S,Bailey JE

更新日期：1991-04-05 00:00:00

abstract：:Alginate gels produced by an external or internal gelation technique were studied so as to determine the optimal bead matrix within which DNA can be immobilized for in vivo application. Alginates were characterized for guluronic/mannuronic acid (G/M) content and average molecular weight using 1H-NMR and LALLS analysis...

journal_title：Biotechnology and bioengineering

authors： Quong D,Neufeld RJ,Skjåk-Braek G,Poncelet D

更新日期：1998-02-20 00:00:00

abstract：:In our preceding article, we demonstrated a procedure based upon enzymic flow microcalorimetry using an enzyme thermistor (ET) to characterize the microkinetic properties of an immobilized enzyme (IME) and its further application in the screening of IMEs. To consider the ET method (single ET unit, ET system 1) as stan...

journal_title：Biotechnology and bioengineering

authors： Gemeiner P,Docolomansky P,Nahálka J,Stefuca V,Danielsson B

更新日期：1996-01-05 00:00:00

abstract：:The productivity of Escherichia coli as a producer of recombinant proteins is affected by its metabolic properties, especially by acetate production. Two commercially used E. coli strains, BL21 (lambdaDE3) and JM109, differ significantly in their acetate production during batch fermentation at high initial glucose con...

journal_title：Biotechnology and bioengineering

authors： van de Walle M,Shiloach J

更新日期：1998-01-05 00:00:00

abstract：:Growth of microorganisms on substitutable substrate mixtures display diverse growth dynamics characterized by simultaneous or preferential uptake of carbon sources. This article shows that cybernetic modeling concepts which were successful in predicting diauxic growth patterns can be extended to describe simultaneous ...

journal_title：Biotechnology and bioengineering

authors： Ramakrishna R,Ramkrishna D,Konopka AE

更新日期：1996-10-05 00:00:00

abstract：:This study describes the development and application of a bioaffinity chromatographic system for the one-step purification of an NADP(+)-dependent secondary alcohol dehydrogenase from the obligate anaerobe, Thermoanaerobacter brockii (TBADH, EC 1.1.1.2). The general approach is based upon improving the selectivity of ...

journal_title：Biotechnology and bioengineering

authors： McMahon M,Mulcahy P

更新日期：2002-03-05 00:00:00

abstract：:This study investigates the scaling of photobioreactor productivity based on the growth of Nannochloropsis salina incorporating the effects of direct and diffuse light. The scaling and optimization of photobioreactor geometry was analyzed by determining the growth response of a small-scale system designed to represent...

journal_title：Biotechnology and bioengineering

authors： Quinn JC,Turner CW,Bradley TH

更新日期：2012-02-01 00:00:00

abstract：:Using solid-phase organic synthesis, we have prepared a 432-member (18 x 8 x 3) sample library based on the AG 490 "tyrphostin" template. By utilizing 432 reactors each equipped with a unique radiofrequency memory ID tag, the 432 products could be obtained as discrete entities (i.e., not as mixtures) via 18 + 8 + 3, o...

journal_title：Biotechnology and bioengineering

authors： Shi S,Xiao XY,Czarnik AW

更新日期：1998-01-01 00:00:00

abstract：:Chinese hamster ovary (CHO) cells are characterized by a low glucose catabolic efficiency, resulting in undesirable lactate production. Here, it is hypothesized that such low efficiency is determined by the transport of pyruvate into the mitochondria. The mitochondrial pyruvate carrier (MPC), responsible for introduci...

journal_title：Biotechnology and bioengineering

authors： Bulté DB,Palomares LA,Parra CG,Martínez JA,Contreras MA,Noriega LG,Ramírez OT

更新日期：2020-09-01 00:00:00

abstract：:Cellulolytic fungi, such as Trichoderma reesei, T. lignorum, and Chaetomium cellulolyticum reach a low packing density of mycelia when grown on straw under conditions of solid substrate fermentation. The low packing density is shown to be caused partially by the geometric limitation of the growth of mycelia in the sub...

journal_title：Biotechnology and bioengineering

authors： Laukevics JJ,Apsite AF,Viesturs US,Tengerdy RP

更新日期：1985-12-01 00:00:00