Abstract:
:We developed a novel method to load and unload molecular cargos to and from microtubules (MTs) that move on kinesin-coated surfaces. Quantum dots (Qds) (molecular cargo) connected to 21-mer DNA can be selectively loaded on DNA-conjugated MTs through DNA hybridization. The average velocity of the Qd-loaded MTs (0.43 +/- 0.06 microm s(-1) at 25 degrees C) was comparable to that of control MTs. In addition, MTs conjugated with two types DNA sequences can achieve multiloading of Qds. To unload Qd molecular cargos from MTs, the DNA double helix connecting Qds to MTs were cleaved by an appropriate restriction enzyme. This biomolecular motors-based transport system should enable us to construct nanometer-scale devices such as nanobiosensor, nanofluidic system, or nanomachine.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Taira S,Du YZ,Hiratsuka Y,Uyeda TQ,Yumoto N,Kodaka Mdoi
10.1002/bit.21618subject
Has Abstractpub_date
2008-02-15 00:00:00pages
734-9issue
3eissn
0006-3592issn
1097-0290journal_volume
99pub_type
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