Abstract:
:Chlorophyllide a was coupled with alpha-(3-aminopropyl)-omega-methoxypoly(oxyethylene) (PEG-NH2) to form a PEG-chlorophyllide conjugate through an acid-amide bond. The conjugate catalyzed the reduction of methylviologen in the presence of 2-mercaptoethanol. It also catalyzed the photoreduction of NADP+ or NAD+ in the presence of ascorbate as an electron donor and ferredoxin-NADP+ reductase as the coupling enzyme. Utilizing the reducing power of NADPH generated by PEG-chlorophyllide conjugate under illumination, glutamate was synthesized from 2-oxoglutarate and NH4+ in the presence of glutamate dehydrogenase. PEG-chlorophyllide conjugate was quite stable toward light illumination compared with chlorophyll a. The increase in the molecular weight of PEG in the PEG-chlorophyllide conjugates was accompanied by the enhancement of photostability of the conjugate and also by the increased solubility in the aqueous solution.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Asada H,Itoh T,Kodera Y,Matsushima A,Hiroto M,Nishimura H,Inada Ydoi
10.1002/bit.1029subject
Has Abstractpub_date
2001-01-01 00:00:00pages
86-90issue
1eissn
0006-3592issn
1097-0290pii
10.1002/bit.1029journal_volume
76pub_type
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