Oxygen uptake rates and liver-specific functions of hepatocyte and 3T3 fibroblast co-cultures.

abstract：:An immobilized enzyme reactor has been developed for the degradation of bilirubin as a potential treatment for neonatal jaundice. It utilizes the enzyme bilirubin oxidase from Myrothecium verrucaria, which in the presence of molecular oxygen converts bilirubin to biliverdin and other products that are much less toxic ...

journal_title：Biotechnology and bioengineering

authors： Sung C,Lavin A,Klibanov AM,Langer R

更新日期：1986-10-01 00:00:00

abstract：:The partitioning behavior of viruses in the two-phase aqueous nonionic n-decyl tetra(ethylene oxide) (C10E4) micellar system cannot be fully explained by considering solely the repulsive, steric, excluded-volume interactions that operate between the viruses and the nonionic C10E4 micelles. Specifically, an excluded-vo...

journal_title：Biotechnology and bioengineering

authors： Kamei DT,Liu CL,Haase-Pettingell C,King JA,Wang DI,Blankschtein D

更新日期：2002-04-20 00:00:00

abstract：:Due to its availability, low-price, and high degree of reduction, glycerol has become an attractive carbon source for the production of fuels and reduced chemicals. Using the platform we have established from the identification of key pathways mediating fermentative metabolism of glycerol, this work reports the engine...

journal_title：Biotechnology and bioengineering

authors： Clomburg JM,Gonzalez R

更新日期：2011-04-01 00:00:00

abstract：:A propanologenic (i.e., 1-propanol-producing) bacterium Escherichia coli strain was previously derived by activating the genomic sleeping beauty mutase (Sbm) operon. The activated Sbm pathway branches out of the tricarboxylic acid (TCA) cycle at the succinyl-CoA node to form propionyl-CoA and its derived metabolites o...

journal_title：Biotechnology and bioengineering

authors： Miscevic D,Mao JY,Moo-Young M,Chou CP

更新日期：2020-05-01 00:00:00

abstract：:In this study it is reported the operation of a horizontal-flow anaerobic immobilized biomass (HAIB) reactor under sulfate-reducing condition which was also exposed to different amounts of ethanol and toluene. The system was inoculated with sludge taken from up-flow anaerobic sludge blanket (UASB) reactors treating re...

journal_title：Biotechnology and bioengineering

authors： Cattony EB,Chinalia FA,Ribeiro R,Zaiat M,Foresti E,Varesche MB

更新日期：2005-07-20 00:00:00

abstract：:The biotransformation of benzaldehyde and glucose to L-phenylacetylcarbinol (PAC) using Candida utilis was demonstrated in a solid-liquid two-phase partitioning bioreactor (TPPB) with the aim of reducing substrate, product, and by-product toxicity via sequestration. Previous work in the field had used octanol as the s...

journal_title：Biotechnology and bioengineering

authors： Khan TR,Daugulis AJ

更新日期：2010-11-01 00:00:00

abstract：:Analytical expressions describing convective flow in a continuous arteriovenous hollow fiber hemofilter were developed. In the lumen of the hollow fiber membrane, existing analytical expressions were applied to describe velocity profiles and pressure. For flow in the shell (the extracapillary space separating the fibe...

journal_title：Biotechnology and bioengineering

authors： Moussy Y

更新日期：2000-04-20 00:00:00

abstract：:Rifamycin B is an important polyketide antibiotic used in the treatment of tuberculosis and leprosy. We present results on medium optimization for Rifamycin B production via a barbital insensitive mutant strain of Amycolatopsis mediterranei S699. Machine-learning approaches such as Genetic algorithm (GA), Neighborhood...

journal_title：Biotechnology and bioengineering

authors： Bapat PM,Wangikar PP

更新日期：2004-04-20 00:00:00

abstract：:The biological molecule responsible for the suppression of pyritic sulfur in fine coal simulated froth flotation treated with bacteria was identified. Protein was found to be the most effective agent in pyrite suppression of the three cell components (protein, lipid, and carbohydrate) assayed. Coal recovery and ash re...

journal_title：Biotechnology and bioengineering

authors： Stainthorpe AC

更新日期：1989-02-05 00:00:00

abstract：:This paper presents a new formulation for the analysis of growth kinetics on multiple nutrients. The baseline for the theory is the concept that a noninteractive growth process occurs among perfectly substitutable nutrients if the locus of points of the substrate concentrations producing equal growth rate is linear. A...

journal_title：Biotechnology and bioengineering

authors： Haas CN

更新日期：1994-06-20 00:00:00

abstract：:Centrifugal adsorption technology (CAT) is a new compact, countercurrent technology for efficient adsorption from large liquid streams by using adsorbent particles in the micrometer range. CAT seems particularly suited for the recovery of macromolecules at low concentrations, because the small particle dimensions lead...

journal_title：Biotechnology and bioengineering

authors： Peet DJ,Bisschops MA,van Hateren SH,van der Wielen LA

更新日期：2002-05-05 00:00:00

abstract：:Gene transcription is regulated by transcription factors that can bind to specific regions on DNA. Antigene oligonucleotides (oligos) can bind to specific regions on DNA and form a triplex with the double-stranded DNA. The triplex can competitively inhibit the binding of transcription factors and, as a result, transcr...

journal_title：Biotechnology and bioengineering

authors： Cheng B,Fournier RL,Relue PA

更新日期：2000-11-20 00:00:00

abstract：:Molecular descriptors represent structural and physicochemical features of compounds. They have been extensively used for developing statistical models, such as quantitative structure activity relationship (QSAR) and artificial neural networks (NN), for computer prediction of the pharmacodynamic, pharmacokinetic, or t...

journal_title：Biotechnology and bioengineering

authors： Li ZR,Han LY,Xue Y,Yap CW,Li H,Jiang L,Chen YZ

更新日期：2007-06-01 00:00:00

abstract：:Cultures with immobilized hybridoma cells were performed in fixed bed systems. "Steady state" values for volume-specific substrate uptake and metabolite production rates were determined at various perfusion rates and superficial flow velocities of the medium within the carrier matrix. Data from fixed bed volumes betwe...

journal_title：Biotechnology and bioengineering

authors： Pörtner R,Rössing S,Koop M,Lüdemann I

更新日期：1997-08-05 00:00:00

abstract：:The cell cycle dependency of foreign gene expression in recombinant mouse L cells was investigated. Two different recombinant mouse L cell lines having the glucocorticoid receptor-encoding gene and the lacZ reporter gene were used in this study. The lacZ gene expression was controlled by the glucocorticoid-inducible m...

journal_title：Biotechnology and bioengineering

authors： Gu MB,Todd P,Kompala DS

更新日期：1996-05-05 00:00:00

abstract：:In macroscopic dynamic models of fermentation processes, elementary modes (EM) derived from metabolic networks are often used to describe the reaction stoichiometry in a simplified manner and to build predictive models by parameterizing kinetic rate equations for the EM. In this procedure, the selection of a set of EM...

journal_title：Biotechnology and bioengineering

authors： Hebing L,Neymann T,Engell S

更新日期：2020-07-01 00:00:00

abstract：:The aim of this study was to determine the feasibility of using high pressure CO2 for sterilization of Ginseng powder, as an alternative method to conventional techniques such as gamma-irradiation and ethylene oxide. The Ginseng sample used in this study was originally contaminated with fungi and 5 x 10(7) bacteria/g ...

journal_title：Biotechnology and bioengineering

authors： Dehghani F,Annabi N,Titus M,Valtchev P,Tumilar A

更新日期：2009-02-01 00:00:00

abstract：:It is shown that the mass transfer resistance can significantly distort the linearity of the Lineweaver-Burk plot of the kinetic data for a microbial culture which forms aggregates. For small flocs, the linearity of the Lineweaver-Burk plot is largely retained, but a different slope and intercept will be obtained comp...

journal_title：Biotechnology and bioengineering

authors： Ngian KF,Lin SH,Martin WR

更新日期：1977-12-01 00:00:00

abstract：:The C-terminal lysine variation is commonly observed in biopharmaceutical monoclonal antibodies. This modification can be important since it is found to be sensitive to the production process. The methods commonly used to probe this charge variation, including IEF, cIEF, ion-exchange chromatography, and LC-MS, were ev...

journal_title：Biotechnology and bioengineering

authors： Dick LW Jr,Qiu D,Mahon D,Adamo M,Cheng KC

更新日期：2008-08-15 00:00:00

abstract：:A method was developed for obtaining high signal-to-noise 13C NMR spectra of intracellular compounds in metabolically active cultured cells. The method allows TCA cycle labeling kinetics to be determined in real time without significant oxygen transport limitations. Cells were immobilized on the surface of nonporous m...

journal_title：Biotechnology and bioengineering

authors： Mancuso A,Beardsley NJ,Wehrli S,Pickup S,Matschinsky FM,Glickson JD

更新日期：2004-09-30 00:00:00

abstract：:In the process of producing ethanol from lignocellulosic materials such as wheat straw, compounds that can act inhibitory to enzymatic hydrolysis and to cellular growth may be generated during the pretreatment. Ethanol production was evaluated on pretreated wheat straw hydrolysate using four different recombinant Sacc...

journal_title：Biotechnology and bioengineering

authors： Panagiotou G,Olsson L

更新日期：2007-02-01 00:00:00

abstract：:Numerous studies on poly γ-d-glutamicacid (γ-PGA) production have investigated terrestrial renewable sources for reducing production costs, but there are no studies using waste marine resources so far. We aimed to develop a cost-effective production method of γ-d-PGA by Bacillus sp. SJ-10 using green macroalgae (Ulva ...

journal_title：Biotechnology and bioengineering

authors： Kim JH,Lee JM,Jang WJ,Park HD,Kim YO,Kim CH,Kong IS

更新日期：2019-07-01 00:00:00

abstract：:This article describes a methodology that implements a Markov decision process (MDP) optimization technique in a real time fed-batch experiment. Biological systems can be better modeled under the stochastic framework and MDP is shown to be a suitable technique for their optimization. A nonlinear input/output model is ...

journal_title：Biotechnology and bioengineering

authors： Saucedo VM,Karim MN

更新日期：1997-07-20 00:00:00

abstract：:The use of cell numbers rather than mass to quantify the size of the biotic phase in animal cell cultures causes several problems. First, the cell size varies with growth conditions, thus yields expressed in terms of cell numbers cannot be used in the normal mass balance sense. Second, experience from microbial system...

journal_title：Biotechnology and bioengineering

authors： Nielsen LK,Reid S,Greenfield PF

更新日期：1997-11-20 00:00:00

abstract：:The treatment of large segmental bone defects remains a challenge as infection, delayed union, and nonunion are common postoperative complications. A three-dimensional printed bioresorbable and physiologically load-sustaining graft substitute was developed to mimic native bone tissue for segmental bone repair. Fabrica...

journal_title：Biotechnology and bioengineering

authors： Chung R,Kalyon DM,Yu X,Valdevit A

更新日期：2018-09-01 00:00:00

abstract：:The quality of cassava starch, an important trait in cassava breeding programs, determines its applications in various industries. For example, development of waxy (having a low level of amylose) cassava is in demand. Amylose is synthesized by granule-bound starch synthase I (GBSSI) in plants, and therefore, down-regu...

journal_title：Biotechnology and bioengineering

authors： Zhao SS,Dufour D,Sánchez T,Ceballos H,Zhang P

更新日期：2011-08-01 00:00:00

abstract：:The feasibility of producing ethanol in a continuous system from cellulose using Clostridirrrn thermocellum was investigated. This anaerobic and therniophilic bacterium was able to degrade cellulose directly into ethanol with acetic acid, hydrogen. and carbon dioxide as by-products of this fermentation. The fermentati...

journal_title：Biotechnology and bioengineering

authors： Zertuche L,Zall RR

更新日期：1982-01-01 00:00:00

abstract：:Screening tests of different marine algae biomas types revealed a high passive biosorptive uptake of lead up to 270 mg Pb/g of biomass in some brown marine algae. Members of the order Fucales perfomed particularly well in this descending sequence: Fucus > Ascophyllum > Sargassum. Although decreasing the swelling of we...

journal_title：Biotechnology and bioengineering

authors： Holan ZR,Volesky B

更新日期：1994-05-01 00:00:00

abstract：:A novel method has been developed for the separation of bioproducts from yeast cells. The method uses a combination of physical, chemical, and biological agents such as lytic enzymes, osmotic supports, and spheroplast stabilizers. Using this technique, products (proteins and enzymes) can be released from specific cell...

journal_title：Biotechnology and bioengineering

authors： Huang RB,Andrews BA,Asenjo JA

更新日期：1991-11-01 00:00:00

abstract：:Yeast alcohol dehydrogenase (ADH) solutions (approximately 1 mg/ml, pH 7) were sheared in a coaxial cylindrical viscometer. This was fitted with a lid sealing the contents from the atmosphere and preventing evaporation. At 30 degrees C after a total of 5 hr intermittent shearing at 683 sec-1 no losses of activity were...

journal_title：Biotechnology and bioengineering

authors： Thomas CR,Nienow AW,Dunnill P

更新日期：1979-12-01 00:00:00