Abstract:
:Animal cell culture is widely used in biotechnology for the production of many biological products. In situ microscopes acquire images directly from cell suspensions and analyze the images in matters of cell concentration, cell size distribution and cell morphology. Their applicability was already proven for yeast and suspended mammalian cell cultivations. In this work the in situ microscope was utilized to measure the level of colonization of fibroblasts on microcarrier surfaces during cultivation. For this study the murine cell line NIH-3T3 was used in combination with Cytodex 1 microcarriers. Cultivations were carried out in a 5 L stirred tank bioreactor equipped with the in situ microscope. Images were obtained sequentially with the in situ microscope over the whole cultivation time (900 images per sequence, 7.5 h per sequence on average). For the microcarrier analysis an image analysis algorithm based on a neural network was developed and implemented in the microscope analysis software.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Rudolph G,Lindner P,Gierse A,Bluma A,Martinez G,Hitzmann B,Scheper Tdoi
10.1002/bit.21523subject
Has Abstractpub_date
2008-01-01 00:00:00pages
136-45issue
1eissn
0006-3592issn
1097-0290journal_volume
99pub_type
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