Abstract:
:Somatic embryogenesis is among the most promising means of large scale plant micropropagation. The development of somatic embryos is characterized by their morphological changes. Embryos in culture usually exhibit high heterogeneity and abnormality. As conventional microscopic observation is laborious and subjective, an objective and quantitative morphokinetic description is important for further advancement of this important process technology. We developed an image analysis system capable of measuring morphological and size features of embryos. Subtle environmental effects on embryo development, which are often masked by the subjectivity of microscopic observation, are now discernible by statistically comparing the distributions of these morphological and size features. This image analysis and pattern recognition system was applied to examine the kinetics of a fed-batch culture.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Chi CM,Zhang C,Staba EJ,Cooke TJ,Hu WSdoi
10.1002/(SICI)1097-0290(19960405)50:1<65::AID-BIT8subject
Has Abstractpub_date
1996-04-05 00:00:00pages
65-72issue
1eissn
0006-3592issn
1097-0290journal_volume
50pub_type
杂志文章abstract::This article describes a method for the chemical immobilization of concanavalin A (Con A) on the inside wall of a single hollow cellulose fiber for use in glucose affinity sensor. Periodate oxidation of cellulose fiber followed by a spacer for Con A attachment was deemed to be the most optimal procedure for achieving ...
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