Relative contributions of biological and chemical reactions to the overall rate of pyrite oxidation at temperatures between 30 degrees C and 70 degrees C.

abstract：:A dynamic model for the degradation of phenol in a two-phase partitioning bioreactor has been developed based on mechanistic balances around the bioreactor. The key process characteristics including substrate transfer between the organic and aqueous phases, substrate inhibition, oxygen limitation, and cell entrainment...

journal_title：Biotechnology and bioengineering

authors： Cruickshank SM,Daugulis AJ,McLellan PJ

更新日期：2000-01-20 00:00:00

abstract：:Invertase from Candida utilis was immobilized on porous cellulose beads by an ionic-quanidino bond. The immobilized invertase showed optimum activity between pH 4.0 and 5.4, while the free enzyme had a sharp optimum at pH 4.1. Both temperature profiles were fairly similar up to 55 degrees C. However, above this temper...

journal_title：Biotechnology and bioengineering

authors： Dickensheets PA,Chen LF,Tsao GT

更新日期：1977-03-01 00:00:00

abstract：:NS0, a nonsecreting mouse myeloma cell, is a major host line used for recombinant antibody production. These cells have a cholesterol-dependent phenotype and rely on an exogenous supply of cholesterol for their survival and growth. To better understand the physiology underlying cholesterol dependence, we compared NS0 ...

journal_title：Biotechnology and bioengineering

authors： Seth G,Philp RJ,Denoya CD,McGrath K,Stutzman-Engwall KJ,Yap M,Hu WS

更新日期：2005-06-05 00:00:00

abstract：:Ralstonia eutropha grows on and produces polyhydroxyalkanoates (PHAs) from fermentation acids. Acetic acid, one major organic acid from acidogenesis of organic wastes, has an inhibitory effect on the bacterium at slightly alkaline pH (6 g HAc/L at pH 8). The tolerance of R. eutropha to acetate, however, was increased ...

journal_title：Biotechnology and bioengineering

authors： Yu J,Wang J

更新日期：2001-06-20 00:00:00

abstract：:RNA viruses are capable of undergoing extremely rapid evolution due to their high rates of reproduction, small genome size, and a high frequency of spontaneous mutagenesis. Here we demonstrate that a virus-like, evolutionary state can be created by propagating a phagemid population in a hypermutator strain of Escheric...

journal_title：Biotechnology and bioengineering

authors： Long-McGie J,Liu AD,Schellenberger V

更新日期：2000-04-05 00:00:00

abstract：:Liquid membrane bilayers, generated by bacteriorhodopsin on a supporting membrane, exhibit photo osmosis. The phenomenon has been shown to be a consequence of light-induced electrical potential differences which develop across the liquid membrane bilayer due to the light-driven proton pumping action of bacteriorhodops...

journal_title：Biotechnology and bioengineering

authors： Srivastava RC,Tandon A

更新日期：1988-04-20 00:00:00

abstract：:Increases in our understanding of gene function have greatly expanded the repertoire of possible genetic interventions at our disposal with the consequence that many genetic engineering applications require multiple manipulations in which target genes can be both overexpressed and silenced in a simple and co-ordinated...

journal_title：Biotechnology and bioengineering

authors： Greber D,Fussenegger M

更新日期：2007-04-01 00:00:00

abstract：:For the commercial exploitation of lipase biocatalysis to be successful, it is essential that effective supports are selected for lipase immobilization. In this study hydrophobic controlled-pore glasses have been used as model systems for the immobilization of Rhizomucor miehei lipase. The effect of pore diameter and ...

journal_title：Biotechnology and bioengineering

authors： Bosley JA,Clayton JC

更新日期：1994-04-25 00:00:00

abstract：:Lectin-like function was demonstrated in this study for a novel water-soluble polymer with phenylboronic acid residues (poly (AAPBA-DMAm)), which induced appreciable proliferation of murine spleen lymphocytes with an increased expression of interleukin-2 (IL-2) receptor on their surface. Consequently, boosted prolifer...

journal_title：Biotechnology and bioengineering

authors： Uchimura E,Otsuka H,Okano T,Sakurai Y,Kataoka K

更新日期：2001-02-05 00:00:00

abstract：:The effects of growing Clostridium acetobutylicum NCIB 8052 in a chemostat under conditions of glucose, NH(4) (+), PO(4) (3-), Mg(2+), and Fe(2+) limitation were examined. It was noted that limitation of any major nutrient resulted in the same fermentation pattern. Conditions where minor nutrient levels were reduced a...

journal_title：Biotechnology and bioengineering

authors： McNeil B,Kristiansen B

更新日期：1987-02-01 00:00:00

abstract：:The simultaneous saccharification and co-fermentation (SSCF) kinetic model described in the companion paper can predict batch and fed batch fermentations well at solids concentrations up to 62.4 g/L cellulose paper sludge but not in batch fermentation at 82.0 g/L cellulose paper sludge. Four hypotheses for the discrep...

journal_title：Biotechnology and bioengineering

authors： Zhang J,Shao X,Lynd LR

更新日期：2009-12-01 00:00:00

abstract：:The attrition bioreactor (ABR) combines wet ball milling and enzymatic hydrolysis in one process step. It was found that the ABR did not accelerate enzyme deactivation. Interfacial forces, not shear forces, caused the most deactivation. Elimination of the air-liquid interface by covering the reactor substantially incr...

journal_title：Biotechnology and bioengineering

authors： Jones EO,Lee JM

更新日期：1988-01-01 00:00:00

abstract：:In this work, novel conductive composite nanofiber mesh possessing reactive groups was electrospun from solutions containing poly(acrylonitrile-co-acrylic acid) (PANCAA) and multi-walled carbon nanotubes (MWCNTs) for redoxase immobilization, assuming that the incorporated MWCNTs could behave as electrons transferor du...

journal_title：Biotechnology and bioengineering

authors： Wang ZG,Ke BB,Xu ZK

更新日期：2007-07-01 00:00:00

abstract：:A biosensor model was developed for a commercial NADH fluorescence probe to describe the single-frequency excitation and emission fluorescence behavior of an aqueous mixture of fluorophores. This model is essential in correlating the measured signals to the concentrations of fluorescent compounds in a bioreactor. In a...

journal_title：Biotechnology and bioengineering

authors： Wang NS,Simmons MB

更新日期：1991-12-20 00:00:00

abstract：:A protein loaded three-dimensional scaffold can be used for protein delivery and bone tissue regeneration. The main objective of this project was to develop recombinant human bone morphogenetic protein-2 (rhBMP-2) loaded poly(D,L-lactide-co-glycolide)/hydroxylapatite (PLGA/HAp) composite fibrous scaffolds through a pr...

journal_title：Biotechnology and bioengineering

authors： Nie H,Soh BW,Fu YC,Wang CH

更新日期：2008-01-01 00:00:00

abstract：:We have used a novel approach in conjunction with flow cytometry to quantify the biological heterogeneity of populations of the ciliate Tetrahymena pyriformis. It was found that the rate of particle uptake of exponentially growing cells is not uniform among cells and partially correlated with cell size. The physiologi...

journal_title：Biotechnology and bioengineering

authors： Hatzis C,Srienc F,Fredrickson AG

更新日期：1994-03-05 00:00:00

abstract：:Gene expression microarrays are a relatively new technology, dating back just a few years, yet they have already become a very widely used tool in biology, and have evolved to a wide range of applications well beyond their original design intent. However, while the use of microarrays has expanded, and the issues of pe...

journal_title：Biotechnology and bioengineering

authors： Grant GR,Manduchi E,Pizarro A,Stoeckert CJ Jr

更新日期：2003-12-30 00:00:00

abstract：:The inhibition of the maximum specific growth and fermentation rate of Zymomonas mobilis by ethanol was studied in turbidostat cultures at constant and stepwise changed ethanol concentrations. Up to 50 g/L ethanol, the inhibition kinetics can be approximated by a linear relationship between the specific growth rate an...

journal_title：Biotechnology and bioengineering

authors： Jöbses IM,Roels JA

更新日期：1986-04-01 00:00:00

abstract：:Highly efficient antibody immobilization is extremely crucial for the development of high-performance polymeric microdevices for enzyme-linked immunosorbent assay (ELISA). In this article, a site-selective tyrosinase (TR)-catalyzed protein A strategy for antibody immobilization was developed to enhance the sensitivity...

journal_title：Biotechnology and bioengineering

authors： Yuan Y,He H,Lee LJ

更新日期：2009-02-15 00:00:00

abstract：:The release kinetics of beta-galactosidase protein have been determined during small-scale ultrasonication of E. coli cells. Among several studied parameters, ionic strength and cell concentration have the least influence on the rate of protein recovery, whereas sample volume and acoustic power dramatically affect the...

journal_title：Biotechnology and bioengineering

authors： Feliu JX,Cubarsi R,Villaverde A

更新日期：1998-06-05 00:00:00

abstract：:In this study, the fragmentation activities of Thermomonospora fusca cellulases E(2), E(3), E(5), Trichoderma reesei CBHI, and their mixtures were measured to study synergism in fragmentation. Fragmentation studies revealed that only two pure cellulases, T. fusca E(2) and E(5) had significant fragmentation activity. T...

journal_title：Biotechnology and bioengineering

authors： Walker LP,Wilson DB,Irvin DC,McQuire C,Price M

更新日期：1992-11-01 00:00:00

abstract：:A two-stage bioreactor scheme was developed for the large-scale production of recombinant proteins using a genetically engineered baculovirus/insect cell system. The first bioreactor was employed for cell growth and the second for cell infection. Silkworm Bm5 cells were infected with a recombinant baculovirus, BmNPV/P...

journal_title：Biotechnology and bioengineering

authors： Zhang J,Kalogerakis N,Behie LA,Iatrou K

更新日期：1993-07-01 00:00:00

abstract：:Aggregates of Escherichia coli confined within hollowfiber reactors were either formed in place by culturing cells within the reactors, or were prepared by injecting a cell suspension that had been concentrated by centrifugation. The effective diffusive permeability of an uncharged nonreacting tracer, nitrous oxide, w...

journal_title：Biotechnology and bioengineering

authors： Libicki SB,Salmon PM,Robertson CR

更新日期：1988-06-20 00:00:00

abstract：:Immobilization of Streptomyces phaerochromogenes was studied by radiation-induced polymerization of 2-hydroxyethyl methacrylate at low temperatures. Radiation damage of the enzyme could be avoided by choosing irradiation at low temperatures. The enzymatic activity of immobilized cells increased remarkably with a decre...

journal_title：Biotechnology and bioengineering

authors： Kumakura M,Yoshida M,Kaetsu I

更新日期：1979-04-01 00:00:00

abstract：:Expanded bed adsorption is an integrated technology that allows the introduction of particle-containing feedstock without the risk of blocking the bed. The biomass particles contained in the feedstock have to be treated as an integral part of the process and potential interactions between suspended biomass and the ads...

journal_title：Biotechnology and bioengineering

authors： Lin DQ,Brixius PJ,Hubbuch JJ,Thömmes J,Kula MR

更新日期：2003-07-20 00:00:00

abstract：:A procedure for measuring the rate of heat production from a fermentation has been developed. The method is based on measuring the rate of temperature rise of the fermentation broth resulting from metabolism, when the temperature controller is turned off. The heat accumulation measured in this manner is then corrected...

journal_title：Biotechnology and bioengineering

authors： Cooney CL,Wang DI,Mateles RI

更新日期：2000-03-20 00:00:00

abstract：:Trehalase is the enzyme which hydrolyzes the disaccharide trehalose into two alpha-D-glucose molecules. In this article, we present the immobilization of trehalase on aminopropyl glass particles. The enzyme was extracted from Escherichia coli Mph2, a strain harboring the pTRE11 plasmid, which contains the trehalase ge...

journal_title：Biotechnology and bioengineering

authors： Bachinski N,Martins AS,Paschoalin VM,Panek AD,Paiva CL

更新日期：1997-04-05 00:00:00

abstract：:In analyzing microbial cellulose utilization, it would be useful to independently measure the mass concentration of cells and cellulase enzymes. Such measurements would allow investigation of the allocation of cellular resources between synthesis of cells and cellulase, in vivo cell- and cellulase-specific cellulose h...

journal_title：Biotechnology and bioengineering

authors： Lynd LR,Zhang Y

更新日期：2002-02-15 00:00:00

abstract：:The possibility of enhancing the biomass productivity of a continuous culture of Saccharomyces cerevisiae growing on a glucose-limited medium is addressed. An unstructured Monod-type model is first identified using steady-state data. The culture is subjected to step changes in dilution rate, and it is seen that the Mo...

journal_title：Biotechnology and bioengineering

authors： Abulesz EM,Lyberatos G

更新日期：1989-09-01 00:00:00

abstract：:Flow cytometry is a fast and sensitive method that allows monitoring of different cellular parameters on large samples of a population. Protein distributions give relevant information on growth dynamics, since they are related to the age distribution and depend on the law of growth of the population and the law of pro...

journal_title：Biotechnology and bioengineering

authors： Alberghina L,Mariani L,Martegani E,Vanoni M

更新日期：1983-05-01 00:00:00