Rapid in vivo evolution of a beta-lactamase using phagemids.

abstract：:We originally investigated the suitability of chitosan scaffolds loaded with bone morphogenetic protein 6 (BMP-6) in both stationary and dynamic conditions for cartilage tissue engineering. In the first part of the present study, ATDC5 murine chondrogenic cells were seeded in chitosan and BMP-6 loaded chitosan scaffol...

journal_title：Biotechnology and bioengineering

authors： Tiğli RS,Gümüşderelioğlu M

更新日期：2009-10-15 00:00:00

abstract：:James (Jay) E. Bailey was a pioneer in biotechnology and biochemical engineering. During his 30 years in academia he made seminal contributions to many fields of chemical engineering science, including catalysis and reaction engineering, bioprocess engineering, mathematical modeling of cellular processes, recombinant ...

journal_title：Biotechnology and bioengineering

authors： Dordick JS,Khosla C

更新日期：2002-09-05 00:00:00

abstract：:Adsorption of cellulases onto lignin is considered a major factor in retarding enzymatic cellulose degradation of lignocellulosic biomass. However, the adsorption mechanisms and kinetics are not well understood for individual types of cellulases. This study examines the binding affinity, kinetics of adsorption, and co...

journal_title：Biotechnology and bioengineering

authors： Djajadi DT,Pihlajaniemi V,Rahikainen J,Kruus K,Meyer AS

更新日期：2018-12-01 00:00:00

abstract：:A model for radial flow mammalian cell culture has been developed. Two situations, one with permeable hollow fibers and the other with a porous matrix in the annulus, were considered. The hollow fibers were modeled as continuously distributed sinks. Numerical solutions are presented for the complete model as well as l...

journal_title：Biotechnology and bioengineering

authors： Tharakan JP,Chau PC

更新日期：1987-04-01 00:00:00

abstract：:Alcoholic fermentation of cheese whey permeate was investigated using a recombinant flocculating Saccharomyces cerevisiae, expressing the LAC4 (coding for beta-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus enabling for lactose metabolization. Data on yeast fermentation and gro...

journal_title：Biotechnology and bioengineering

authors： Domingues L,Lima N,Teixeira JA

更新日期：2001-03-05 00:00:00

abstract：:The continuous separation of proteins was performed in a countercurrent gradient chromatography (CGC) system. A magnetically stabilized fluidized bed (MSFB) was used to establish true countercurrent contact of a solid resin with a liquid buffer. STable pH gradients were formed in the system in less than 10 min and rem...

journal_title：Biotechnology and bioengineering

authors： Evans LL,Burns MA

更新日期：1995-12-05 00:00:00

abstract：:Recently, we reported that the loss of a telomeric region of chromosome 8 in Chinese Hamster Ovary (CHO) cells correlates with higher recombinant productivities. New cell lines lacking this region, called CHO-C8DEL, showed several advantages during cell line generation and for the production of recombinant proteins (R...

journal_title：Biotechnology and bioengineering

authors： Ritter A,Rauschert T,Oertli M,Piehlmaier D,Mantas P,Kuntzelmann G,Lageyre N,Brannetti B,Voedisch B,Geisse S,Jostock T,Laux H

更新日期：2016-11-01 00:00:00

abstract：:The gp41 polypeptide of human immunodeficiency virus (HIV) contains an immunosuppressive domain, an epitope which elicits specific cytolytic T cell responses to HIV, and a complement Clq interactive domain. In addition, a synthetic peptide called CS3, derived from gp41 (amino acids 576-593 of gp160) and contiguous wit...

journal_title：Biotechnology and bioengineering

authors： Kestler DP,Henderson LA,Noti JD

更新日期：1993-06-05 00:00:00

abstract：:The glycosylation of pharmaglycoproteins from recombinant cell lines can be affected by an uncontrolled accumulation of ammonium in the medium. Glucosamine-6-phosphate isomerase (GPI) has been proposed as the key enzyme responsible for elevating the intracellular UDP-N-acetylhexosamine pool (UDPGNAc) by accepting ammo...

journal_title：Biotechnology and bioengineering

authors： Cayli A,Hirschmann F,Wirth M,Hauser H,Wagner R

更新日期：1999-10-20 00:00:00

abstract：:Growth of microorganisms on substitutable substrate mixtures display diverse growth dynamics characterized by simultaneous or preferential uptake of carbon sources. This article shows that cybernetic modeling concepts which were successful in predicting diauxic growth patterns can be extended to describe simultaneous ...

journal_title：Biotechnology and bioengineering

authors： Ramakrishna R,Ramkrishna D,Konopka AE

更新日期：1996-10-05 00:00:00

abstract：:Protein aggregation of monoclonal antibodies (mAbs) is a common phenomenon associated with the production of these biopharmaceuticals. These aggregates can lead to adverse side effects in patients upon administration, thus expensive downstream processing steps to remove the higher molecular weight species are inevitab...

journal_title：Biotechnology and bioengineering

authors： Paul AJ,Handrick R,Ebert S,Hesse F

更新日期：2018-05-01 00:00:00

abstract：:A comparative study of the performance of solid and liquid non-aqueous phases (NAPs) to enhance the mass transfer and biodegradation of hexane by Pseudomonas aeruginosa in two-phase partitioning bioreactors (TPPBs) was undertaken. A preliminary NAP screening was thus carried out among the most common solid and liquid ...

journal_title：Biotechnology and bioengineering

authors： Hernández M,Quijano G,Thalasso F,Daugulis AJ,Villaverde S,Muñoz R

更新日期：2010-08-01 00:00:00

abstract：:Power densities and oxidation-reduction potentials (ORPs) of MFCs containing a pure culture of Shewanella oneidensis MR-1 were compared to mixed cultures (wastewater inoculum) in cube shaped, 1-, 2-, and 3-bottle batch-fed MFC reactor configurations. The reactor architecture influenced the relative power produced by t...

journal_title：Biotechnology and bioengineering

authors： Watson VJ,Logan BE

更新日期：2010-02-15 00:00:00

abstract：:The microscopic morphology of Trichoderma reesei QM 9414, growing in submerged culture, was studied by image analysis. The morphology was characterized by the total hyphal length, the total number of tips, the number of actively growing tips, and the length of the main hypha. To describe the growth of a single myceliu...

journal_title：Biotechnology and bioengineering

authors： Lejeune R,Nielsen J,Baron GV

更新日期：1995-09-05 00:00:00

abstract：:CTLA4 is a membrane receptor on cytotoxic T cells whose interaction with the B7 counterreceptor on B cells is important in alloantigen responses. Soluble recombinant human and murine CTLA4 were produced using either Chinese hamster ovary or NS-0 cell lines. Expression vectors were constructed containing the gene codin...

journal_title：Biotechnology and bioengineering

authors： Flesher AR,Marzowski J,Wang WC,Raff HV

更新日期：1995-06-05 00:00:00

abstract：:Remarkable increases in enzyme catalytic stability resulting from addition of charged water-soluble polymers have recently been reported, suggesting that use of these polymers may be an attractive general strategy for enzyme stabilization. To test the proposed hypothesis that coulombic forces between water-soluble pol...

journal_title：Biotechnology and bioengineering

authors： Foreman TM,Khalil M,Meier P,Brainard JR,Vanderberg LA,Sauer NN

更新日期：2001-11-01 00:00:00

abstract：:Methyl parathion hydrolase (MPH) has been displayed on the surface of microorganisms for the first time using only N- and C-terminal domains of the ice nucleation protein (INPNC) from Pseudomonas syringae INA5 as an anchoring motif. A shuttle vector pINCM coding for INPNC-MPH was constructed and used to target MPH ont...

journal_title：Biotechnology and bioengineering

authors： Yang C,Cai N,Dong M,Jiang H,Li J,Qiao C,Mulchandani A,Chen W

更新日期：2008-01-01 00:00:00

abstract：:Three micronization techniques, based on the use of supercritical carbon dioxide, were investigated to produce microspheres of a natural biocompatible polysaccharide. Particles smaller than 20 mum were obtained by means of the rapid expansion of a supercritical solution method (RESS), both with and without cosolvents....

journal_title：Biotechnology and bioengineering

authors： Benedetti L,Bertucco A,Pallado P

更新日期：1997-01-20 00:00:00

abstract：:The implementation of control algorithms to batch bioreactors is often complicated by variations in process dynamics that occur during the course of fermentation. Such a wide operating range often renders the performance of fixed gain proportional-integral-differential (PID) controllers unsatisfactory. In this work, d...

journal_title：Biotechnology and bioengineering

authors： Cardello RJ,San KY

更新日期：1988-08-05 00:00:00

abstract：:The kinetics and mechanism of Fe(III) reduction to Fe(II) were studied in pure batch cultures of Pseudomonas sp. 200. The rate of iron reduction has been mechanistically related to aqueous phase iron speciation. In the absence of microbial activity the iron reduction rate was negligible. Initial rates of microbial iro...

journal_title：Biotechnology and bioengineering

authors： Arnold RG,Olson TM,Hoffmann MR

更新日期：1986-11-01 00:00:00

abstract：:Surface modified bioactive glass with surface properties akin to those of the bone mineral phase is an attractive candidate for use as a microcarrier material for 3-D growth of bone-like tissue in rotating wall vessel bioreactors (RWVs). The critical surface properties of this material are the result of reaction in so...

journal_title：Biotechnology and bioengineering

authors： Radin S,Ducheyne P,Ayyaswamy PS,Gao H

更新日期：2001-11-05 00:00:00

abstract：:Recycle batch fermentations using immobilized cells of Propionibacterium acidipropionici were studied for propionate production from whey permeate, de-lactose whey permeate, and acid whey. Cells were immobilized in a spirally wound fibrous sheet packed in a 0.5-L column reactor, which was connected to a 5-L stirred ta...

journal_title：Biotechnology and bioengineering

authors： Yang ST,Huang Y,Hong G

更新日期：1995-03-05 00:00:00

abstract：:Human tissue-type plasminogen activator (t-PA) contains a variably occupied glycosylation site at Asn-184 in naturally produced t-PA and in t-PA produced in recombinant Chinese hamster ovary (CHO) cells. The presence of an oligosaccharide at this site has previously been shown to reduce specific activity and fibrin bi...

journal_title：Biotechnology and bioengineering

authors： Andersen DC,Bridges T,Gawlitzek M,Hoy C

更新日期：2000-10-05 00:00:00

abstract：:The Ff filamentous bacteriophages show potential as a new class of therapeutics, displaying utility in materials science as well as pharmaceutical applications. These phages are produced by the infection of E. coli, a Gram-negative bacterium which unavoidably sheds endotoxins into the extracellular space during growth...

journal_title：Biotechnology and bioengineering

authors： Branston SD,Wright J,Keshavarz-Moore E

更新日期：2015-08-01 00:00:00

abstract：:Biofilm growth in porous media is difficult to study non-invasively due to the opaqueness and heterogeneity of the systems. Magnetic resonance is utilized to non-invasively study water dynamics within porous media. Displacement-relaxation correlation experiments were performed on fluid flow during biofilm growth in a ...

journal_title：Biotechnology and bioengineering

authors： Vogt SJ,Sanderlin AB,Seymour JD,Codd SL

更新日期：2013-05-01 00:00:00

abstract：:An examination is made of five methods for obtaining values of the enthalpy of formation of a unit mass of living Escherichia coli K-12 cells. The values obtained by these methods ranged from -88.95 kJ to -99.55 kJ, the gross average being 96.01 kJ, per unit carbon formula weight equivalent of living, hydrated cells. ...

journal_title：Biotechnology and bioengineering

authors： Battley EH

更新日期：1992-01-05 00:00:00

abstract：:A poly(acrylic acid)-polyethylene graft copolymer was prepared and used initially to couple to acid phosphatase, using soluble carbodiimides. Yields which were quite good were obtained with CMC but not with EDAC. The copolymers was used to couple trypsin using EEDQ. Several organic solvents were investigated for the p...

journal_title：Biotechnology and bioengineering

authors： Beddows CG,Gil MH,Guthrie JT

更新日期：1982-06-01 00:00:00

abstract：:Aspergillus awamori NRRL 4869 was cultured on the solid substrate, wheat bran, in a modified Rollacell apparatus to produce alpha-galactosidase and invertase. The swivel cap on the elongated bottle permits the introduction of air while the bottle rotates. Parameters of air flow rate (0.05-0.2 liter/kg/min), rpm (0.15-...

journal_title：Biotechnology and bioengineering

authors： Silman RW

更新日期：1980-02-01 00:00:00

abstract：:The copolymerization method of immobilization was used to obtain preparations of enzymes covalently incorporated in polyacrylamide gel. They possess properties making them suitable for practical use. First, the preparations are hundreds of times more stable against irreversible thermoinactivation than native enzymes. ...

journal_title：Biotechnology and bioengineering

authors： Mozhaev VV,Siksnis VA,Torchilin VP,Martinek K

更新日期：1983-08-01 00:00:00

abstract：:Protein-protein interactions were measured for ovalbumin and for lysozyme in aqueous salt solutions. Protein-protein interactions are correlated with a proposed potential of mean force equal to the free energy to desolvate the protein surface that is made inaccessible to the solvent due to the protein-protein interact...

journal_title：Biotechnology and bioengineering

authors： Curtis RA,Ulrich J,Montaser A,Prausnitz JM,Blanch HW

更新日期：2002-08-20 00:00:00