Surface transformation of bioactive glass in bioreactors simulating microgravity conditions. Part I: experimental study.

abstract：:Thermodynamic feasibility analysis (TFA) has been used as a tool capable of providing additional constraints to the mass balance-based methods of analysis of metabolic networks (e.g., flux balance analysis). Several publications have recently appeared in which TFA of different metabolic pathways from relatively simple...

journal_title：Biotechnology and bioengineering

authors： Vojinović V,von Stockar U

更新日期：2009-07-01 00:00:00

abstract：:Capitalizing on components evolved to metabolize ethanol in Aspergillus nidulans, we previously designed the first molecular gas-gene expression interface using gaseous acetaldehyde as the major inducer. This fungus-derived acetaldehyde-inducible gene regulation (AIR) system operated perfectly and enabled precise and ...

journal_title：Biotechnology and bioengineering

authors： Weber W,Spielmann M,Daoud El-Baba M,Keller B,Aubel D,Fussenegger M

更新日期：2005-06-30 00:00:00

abstract：:The G-H loop of foot-and-mouth disease virus is a disordered protrusion of the VP1 protein exposed on the virion surface. This short stretch includes an arginine-glycine-aspartic acid tripeptide, a recognized integrin-binding motif, which is responsible for cell attachment and infection. Eight copies of a peptide repr...

journal_title：Biotechnology and bioengineering

authors： Villaverde A,Feliu JX,Arís A,Harbottle RP,Benito A,Coutelle C

更新日期：1998-08-05 00:00:00

abstract：:Alginate gels produced by an external or internal gelation technique were studied so as to determine the optimal bead matrix within which DNA can be immobilized for in vivo application. Alginates were characterized for guluronic/mannuronic acid (G/M) content and average molecular weight using 1H-NMR and LALLS analysis...

journal_title：Biotechnology and bioengineering

authors： Quong D,Neufeld RJ,Skjåk-Braek G,Poncelet D

更新日期：1998-02-20 00:00:00

abstract：:Lyophilized proteins exhibit "pH memory," i.e., their behavior in the solid form corresponds to the pH of the aqueous solution from which they were freeze dried. Herein, we directly tested whether the ionization state is "remembered" by model organic compounds containing various protein functional groups (amino, carbo...

journal_title：Biotechnology and bioengineering

authors： Costantino HR,Griebenow K,Langer R,Klibanov AM

更新日期：1997-02-05 00:00:00

abstract：:A model is proposed that accounts for the decreases in yield which occur in chemostat cultures of mesophilic yeasts at superoptimal growth temperatures. Two yield depressing effects were identified, one due to increased maintenance requirements by the viable fraction of the population, the other due to energy substrat...

journal_title：Biotechnology and bioengineering

authors： Van Uden N,Madeira-Lopes A

更新日期：1976-06-01 00:00:00

abstract：:We have developed a simple and robust transient expression system utilizing the 25 kDa branched cationic polymer polyethylenimine (PEI) as a vehicle to deliver plasmid DNA into suspension-adapted Chinese hamster ovary cells synchronized in G2/M phase of the cell cycle by anti-mitotic microtubule disrupting agents. The...

journal_title：Biotechnology and bioengineering

authors： Tait AS,Brown CJ,Galbraith DJ,Hines MJ,Hoare M,Birch JR,James DC

更新日期：2004-12-20 00:00:00

abstract：:We have produced Bioglass coatings for Orthopedic implants by using a novel coating technique, CoBlast. The two resultant surfaces, designated BG and hydroxyapatite (HA)/BG, were compared with their HA counterpart, OsteoZip in terms of osteoblastic cell attachment, adhesion, proliferation, differentiation, and growth ...

journal_title：Biotechnology and bioengineering

authors： Tan F,Naciri M,Al-Rubeai M

更新日期：2011-02-01 00:00:00

abstract：:Real-time polymerase chain reaction (PCR) is one of the most sensitive and accurate methods for quantifying transcript levels especially for those expressed at low abundance. The selective amplification of target DNA over multiple cycles allows its initial concentration to be determined. The amplification rate is a co...

journal_title：Biotechnology and bioengineering

authors： Mehra S,Hu WS

更新日期：2005-09-30 00:00:00

abstract：:Kluyveromyces marxianus UCD (FST) 55-82 cells were immobilized in Na alginate beads and used in a packed-bed bioreactor system for the continuous production of ethanol from the extract of Jerusalem artichoke tubers. Volumetric ethanol productivities of 104 and 80 g ethanol/ L/h were obtained at 80 and 92% sugar utiliz...

journal_title：Biotechnology and bioengineering

authors： Margaritis A,Bajpai P

更新日期：1982-07-01 00:00:00

abstract：:Yeast alcohol dehydrogenase (ADH) solutions (approximately 1 mg/ml, pH 7) were sheared in a coaxial cylindrical viscometer. This was fitted with a lid sealing the contents from the atmosphere and preventing evaporation. At 30 degrees C after a total of 5 hr intermittent shearing at 683 sec-1 no losses of activity were...

journal_title：Biotechnology and bioengineering

authors： Thomas CR,Nienow AW,Dunnill P

更新日期：1979-12-01 00:00:00

abstract：:On-line NAD(P)H fluorescence and culture redox potential (CRP) measurements were utilized to investigate the role of Vitreoscilla hemoglobin (VHb) in perturbing oxygen metabolism of microaerobic Escherichia coli Batch cultures of a VHb-synthesizing E. coli strain and the iso-genic control under fully aerated condition...

journal_title：Biotechnology and bioengineering

authors： Tsai PS,Rao G,Bailey JE

更新日期：1995-08-05 00:00:00

abstract：:An experimental study was undertaken to quantify the effects of infection cell density, medium condition, and surface aeration on recombinant protein yields in insect cells. In the absence of surface aeration and fresh medium, insect cells generated higher product yields (on a per cell basis) when infected with recomb...

journal_title：Biotechnology and bioengineering

authors： Lindsay DA,Betenbaugh MJ

更新日期：1992-03-15 00:00:00

abstract：:Two-dimensional image analysis was applied to counting, sizing, and density determinations of granules in full-scale and laboratory-scale upflow anaerobic sludge blanket (UASB) digesters. An advantage of this technique for monitoring laboratory-scale digester sludge is the small amount of material required for analysi...

journal_title：Biotechnology and bioengineering

authors： Dudley BT,Howgrave-Graham AR,Bruton AG,Wallis FM

更新日期：1993-07-01 00:00:00

abstract：:Advanced cell and gene therapies such as chimeric antigen receptor T-cell immunotherapies (CAR-T), present a novel therapeutic modality for the treatment of acute and chronic conditions including acute lymphoblastic leukemia and non-Hodgkin lymphoma. However, the development of such immunotherapies requires the manufa...

journal_title：Biotechnology and bioengineering

authors： Costariol E,Rotondi M,Amini A,Hewitt CJ,Nienow AW,Heathman TRJ,Micheletti M,Rafiq QA

更新日期：2019-10-01 00:00:00

abstract：:A flow cytometric kinetic study of hybridoma growth and monoclonal antibody production is presented, along with the influence of glutamine on intracellular responses such as (relative) cell size, and cell RNA and total protein content. Specific findings are: (1) RNA content remained constant throughout the growth phas...

journal_title：Biotechnology and bioengineering

authors： Dalili M,Ollis DF

更新日期：1990-06-05 00:00:00

abstract：:Continous culture experiments with the obligatory methanotroph, Methylosinus trichosporium OB3b, were conducted to study the whole-cell methane monooxygenase (MMO) and nitrogenase activities in a nitrate minimal salts medium under oxygen-limited conditions with methane as the carbone source. The important variables in...

journal_title：Biotechnology and bioengineering

authors： Shah NN,Park S,Taylor RT,Droege MW

更新日期：1992-09-01 00:00:00

abstract：:A technique has been developed for characterizing the in vivo behavior of key enzymes from intermediate measurements. The technique is based on the identification of characteristic reaction paths, and it depends on the time scale separation characteristics of the systems. It is shown that useful information can be obt...

journal_title：Biotechnology and bioengineering

authors： Liao JC,Lightfoot EN Jr

更新日期：1988-05-20 00:00:00

abstract：:A two-stage deterministic model of the growth of Saccharomyces cerevisiae is presented. The cell cycle of this organism was used to suggest the basic model structure. The model represents the preparatory processes of substrate uptake and conversion separately from replication and division. The regulation of the fracti...

journal_title：Biotechnology and bioengineering

authors： Bijkerk AH,Hall RJ

更新日期：1977-02-01 00:00:00

abstract：:Application of plasmid DNA as pharmaceutical to be used in gene therapy and vaccination has been investigated intensively in recent years. To be able to provide sufficient material that is in accordance with quality of pharmaceutical grade it is mandatory to gain comprehensive process knowledge which is even requested...

journal_title：Biotechnology and bioengineering

authors： Bohle K,Ross A

更新日期：2011-09-01 00:00:00

abstract：:Due to its availability, low-price, and high degree of reduction, glycerol has become an attractive carbon source for the production of fuels and reduced chemicals. Using the platform we have established from the identification of key pathways mediating fermentative metabolism of glycerol, this work reports the engine...

journal_title：Biotechnology and bioengineering

authors： Clomburg JM,Gonzalez R

更新日期：2011-04-01 00:00:00

abstract：:The progression of liver disease from normal to cirrhotic state is characterized by modulation of the stiffness of the extracellular matrix (ECM). Mimicking this modulation in vitro scaffold could provide a better insight into hepatic cell behavior. In this study, interpenetrating poly(N-isopropylacrylamide-co-gelatin...

journal_title：Biotechnology and bioengineering

authors： Sarkar J,Kamble SC,Patil R,Kumar A,Gosavi SW

更新日期：2020-02-01 00:00:00

abstract：:Proper analysis of label distribution in metabolic pathway intermediates is critical for correct interpretation of experimental data and strategic experimental design. While, for example, 13C nuclear magnetic resonance (NMR) spectroscopy is usually limited to the measurement of degrees of 13C enrichment, more informat...

journal_title：Biotechnology and bioengineering

authors： Klapa MI,Park SM,Sinskey AJ,Stephanopoulos G

更新日期：1999-02-01 00:00:00

abstract：:Animal cell cultivation in vitro has been studied for more than 40 years. However, the culture medium composition has not been designed on the basis of the stoichiometric nutritional demands for animal cell growth. In this article, a model was developed to study the stoichiometric demands for nutrients (including gluc...

journal_title：Biotechnology and bioengineering

authors： Xie L,Wang DI

更新日期：1994-05-01 00:00:00

abstract：:A comparative study of the performance of solid and liquid non-aqueous phases (NAPs) to enhance the mass transfer and biodegradation of hexane by Pseudomonas aeruginosa in two-phase partitioning bioreactors (TPPBs) was undertaken. A preliminary NAP screening was thus carried out among the most common solid and liquid ...

journal_title：Biotechnology and bioengineering

authors： Hernández M,Quijano G,Thalasso F,Daugulis AJ,Villaverde S,Muñoz R

更新日期：2010-08-01 00:00:00

abstract：:Oil residues arising from the Christos-Bitas spillage were found to contain 28% of oil extractable by carbon tetrachloride; the remainder consisted of water and undefined solids. Christos-Bitas mousse was added to 1.18 m(3) liquor inoculated with oil-contaminated marine mud, and aerated with a 1.5-hp vortex pump and v...

journal_title：Biotechnology and bioengineering

authors： Berwick PG

更新日期：1985-01-01 00:00:00

abstract：:Protein phosphorylation is an important post-translational modification for intracellular signaling molecules, mostly found in serine and threonine residues. Tyrosine phosphorylations are very few events (less than 0.1% to phosphorylated serine/threonine residues), but capable of governing cell fate decisions involved...

journal_title：Biotechnology and bioengineering

authors： Yoshimoto N,Ikeda Y,Tatematsu K,Iijima M,Nakai T,Okajima T,Tanizawa K,Kuroda S

更新日期：2016-08-01 00:00:00

abstract：:Three micronization techniques, based on the use of supercritical carbon dioxide, were investigated to produce microspheres of a natural biocompatible polysaccharide. Particles smaller than 20 mum were obtained by means of the rapid expansion of a supercritical solution method (RESS), both with and without cosolvents....

journal_title：Biotechnology and bioengineering

authors： Benedetti L,Bertucco A,Pallado P

更新日期：1997-01-20 00:00:00

abstract：:Purified rat liver phenylalanine hydroxylase [L-phenylalanine:tetrahydropteridine:oxygen oxidoreductase (4-hydroxylating), EC 1.14.16.1] was immobilized with activated thiol-Sepharose 4B via disulfide bond formation, which is expected to immobilize the enzyme in its activated form through the SH modification. This imm...

journal_title：Biotechnology and bioengineering

authors： Koizumi S,Takeuchi T,Umezawa H,Nagatsu T

更新日期：1986-04-01 00:00:00

abstract：:A new method is described for the immobilization of biologically active proteins onto several types of organic polymer beads. First, the soluble protein is modified by reaction with an excess of a hydrophobic imidoester, for example methyl 4-phenyl-butyrimidate, at ca. pH 9 and 0 degrees . Excess imidoester and side p...

journal_title：Biotechnology and bioengineering

authors： Ampon K,Means GE

更新日期：1988-08-20 00:00:00