Abstract:
:The inhibition of the maximum specific growth and fermentation rate of Zymomonas mobilis by ethanol was studied in turbidostat cultures at constant and stepwise changed ethanol concentrations. Up to 50 g/L ethanol, the inhibition kinetics can be approximated by a linear relationship between the specific growth rate and the ethanol concentration. Above this level, deviations from this linearity are observed. The specific fermentation rates were less inhibited by ethanol than was the specific growth rate. The maximum ethanol concentration achieved was 72 g/L.The response time for the adaptation of a turbidostat culture to step changes in the ethanol concentration was markedly dependent on the concentration level, the response time being large at high ethanol concentrations.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Jöbses IM,Roels JAdoi
10.1002/bit.260280412subject
Has Abstractpub_date
1986-04-01 00:00:00pages
554-63issue
4eissn
0006-3592issn
1097-0290journal_volume
28pub_type
杂志文章abstract::Two simultaneous fermentations were performed at 26 degrees C with simultaneous inocula using Clostridium saccharoperbutylacetonicum. Fermentation 1 prevented the gas formed by the biomass from escaping the fermentor while 2 allowed the gas formed to escape. Fermentor 1 provided for the production of butanol, acetone,...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260280302
更新日期:1986-03-01 00:00:00
abstract::Lipase (EC 3.1.1.3., from Pseudomonas sp.) was entrapped in collagen membrane containing liquid crystal (4-methoxybenzilidene-4'-n-butylaniline). The activity of the lipase-liquid crystal membrane at an applied voltage of 4 V was 3.4 compared to a membrane tested without imposition of an external electric field. A lin...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260191007
更新日期:1977-10-01 00:00:00
abstract::This article describes a methodology that implements a Markov decision process (MDP) optimization technique in a real time fed-batch experiment. Biological systems can be better modeled under the stochastic framework and MDP is shown to be a suitable technique for their optimization. A nonlinear input/output model is ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19970720)55:2<317::AID-BIT
更新日期:1997-07-20 00:00:00
abstract::Plasmid gene product accumulation in a cell population depends on the fraction of plasmid-containing cells and the distribution of single-cell plasmid content. These important population properties have been related to plasmid replication regulation and kinetics and to plasmid segregation rules at the single-cell leve...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260260519
更新日期:1984-05-01 00:00:00
abstract::Pretreatment of rice straw by using renewable cholinium amino acids ionic liquids ([Ch][AA] ILs)-water mixtures and the subsequent enzymatic hydrolysis of the residues were conducted in the present work. Of the eight mixtures composed of ILs and water, most were found to be effective for rice straw pretreatment. After...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24862
更新日期:2013-07-01 00:00:00
abstract::Fed-batch culture of Bacillus thuringiensis in a modified airlift reactor has been developed by using adaptive control of glucose concentration in the reactor. The glucose concentration was estimated via a correlation equation between carbon dioxide production rate and glucose consumption rate. The estimated glucose c...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260480307
更新日期:1995-11-05 00:00:00
abstract::The study reports on the development of a bioreactor for the production of alpha-keto acids from D,L- or D-amino acids using Rhodotorula gracilis D-amino acid oxidase. D-amino acid oxidase was co-immobilized with catalase on Affi-Gel 10 matrix, and the reactor was operated as a continuous-stirred tank reactor (CSTR) o...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260441104
更新日期:1994-12-01 00:00:00
abstract::Models for the known glucose transporters in Aspergillus niger and for simple diffusion of glucose through the hyphal membrane were prepared. The results from the models were compared with fermentation data from published studies on citric acid. It was found that the purely physical and uncontrolled process of diffusi...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(20000220)67:4<451::aid-bit
更新日期:2000-02-20 00:00:00
abstract::Cancer is the leading cause of death in industrialized countries. Cancer therapy often involves monoclonal antibodies or small-molecule drugs, but carbohydrate-binding lectins such as mistletoe (Viscum album) viscumin offer a potential alternative treatment strategy. Viscumin is toxic in mammalian cells, ruling them o...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27076
更新日期:2019-09-01 00:00:00
abstract::Gluconic acid and sorbitol were simultaneously produced from glucose and Jerusalem artichoke using a glucose-fructose oxidoreductase of Zymomonas mobilis and inulinase. Inulinase was immobilized on chitin by cross-linking with glutaraldehyde. Cells of Z. mobilis permeabilized with toluene were coimmobilized with chiti...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260390312
更新日期:1992-02-05 00:00:00
abstract::Penicillinase (beta-lactamase I, EC 3.5.2.6) secreted by Bacillus cereus, strain 569/H, was covalently attached to aminoethyl cellulose via glutaraldehyde. The immobilized derivative shows increased thermostability and decreased susceptibility to conformational changes induced by certain substrates of penicillinase. T...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260210512
更新日期:1979-05-01 00:00:00
abstract::Growth decoupling can be used to optimize the production of biochemicals and proteins in cell factories. Inhibition of excess biomass formation allows for carbon to be utilized efficiently for product formation instead of growth, resulting in increased product yields and titers. Here, we used CRISPR interference to in...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27536
更新日期:2020-08-18 00:00:00
abstract::Overexpression of the cyclin-dependent kinase inhibitor p27 and exposure to low temperature (30 degrees C) represent two strategies to establish controlled proliferation processes for production of therapeutic proteins using Chinese hamster ovary (CHO) cells. Here we analyze the effect of growth inhibition on the qual...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:2001-03-20 00:00:00
abstract::The selectivity of protein extraction by reversed micellar solutions can be improved by the addition of affinity cosurfactants bearing ligands which bind strongly to the target protein. The interactions between cosurfactant and protein, as well as the interfacial activity of both the free cosurfactant and the protein-...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260421011
更新日期:1993-11-20 00:00:00
abstract::Mevalonate is a useful metabolite synthesized from three molecules of acetyl-CoA, consuming two molecules of NADPH. Escherichia coli ( E. coli) catabolizes glucose to acetyl-CoA via several routes, such as the Embden-Meyerhof-Parnas (EMP) and the oxidative pentose phosphate (oxPP) pathways. Although the oxPP pathway s...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26923
更新日期:2019-05-01 00:00:00
abstract::Fungal biofilters have been recently studied as an alternative to the bacterial systems for the elimination of hydrophobic volatile organic compounds (VOC). Fungi foster reduced transport limitation of hydrophobic VOCs due to their hydrophobic surface and extended gas exchange area associated to the hyphal growth. Nev...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.23003
更新日期:2011-04-01 00:00:00
abstract::Connexin-43 (Cx43) containing giant liposomes (GL) were prepared by a baculovirus expression-liposome fusion method. Recombinant budded viruses expressing Cx43 were prepared and then fused with GLs containing DOPG/DOPC at pH 4.5. Connexon formation on the GL membrane was observed by transmission electron microscope. H...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22845
更新日期:2010-12-01 00:00:00
abstract::This work investigated the formation of microbial granules to boost the productivity of lactic acid (LA). The flocculated form of LA-producing microbial consortium, dominated by Lactobacillus sp. (91.5% of total sequence), was initially obtained in a continuous stirred-tank reactor (CSTR), which was fed with 2% glucos...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25540
更新日期:2016-01-01 00:00:00
abstract::Microcrystalline cellulose (10 g/L Avicel) was hydrolysed by two major cellulases, cellobiohydrolase I (CBH I) and endoglucanase II (EG II), of Trichoderma reesei. Two types of experiments were performed, and in both cases the enzymes were added alone and together, in equimolar mixtures. In time course studies the rea...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:1998-09-05 00:00:00
abstract::Gluconobacter melanogenus IFO 3293 cells capable of converting L-sorbose to L-sorbosone were immobilized in polyacrylamide gel. The preferred polymer composition for high activity and stability was determined to contain a total monomer concentration of 7.2% and 16.6% crosslinking agent. No significant differences in o...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260180208
更新日期:1976-02-01 00:00:00
abstract::Glucose oxidase, invertase, and amyloglucosidase were entrapped in calcium alginate gels as concanavalin A complexes in order to prevent the leaching out of the enzymes from the porous matrix. The free as well as the gel-entrapped concanavalin A-glycoenzyme complexes exhibited a relatively high effectiveness factor, e...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260270803
更新日期:1985-08-01 00:00:00
abstract::In this study, a non-sterile (open) continuous fermentation (OCF) process with no-carbon loss was developed to improve lactic acid (LA) productivity and operational stability from the co-utilization of lignocellulose-derived sugars by thermophilic Enterococcus faecium QU 50. The effects of different sugar mixtures on ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27313
更新日期:2020-06-01 00:00:00
abstract::Recombinant retroviruses are effective vectors able to integrate transgenes into the target cell's genome to achieve longer-term expression. This study investigates the effect of cell lysis products, a common cell culture by-product, on the transduction of suspension cells by gammaretroviral vectors. Cell lysates deri...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22634
更新日期:2010-04-15 00:00:00
abstract::Recent studies have demonstrated the feasibility of using ultrafiltration for the purification of plasmid DNA, but there is still little understanding of the factors governing DNA transmission. Experimental data were obtained for the transmission of a 3.0 kbp supercoiled plasmid DNA through composite regenerated cellu...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21575
更新日期:2008-02-01 00:00:00
abstract::An immobilized enzyme (pancreatic ribonuclease bound to porous titania) was investigated for the degradation of purified yeast ribonucleic acid as a substrate. The immobilized enzyme is active and stable in the pH range 4--8. Dependence of enzymatic activity on ionic strength, pH, temperature, fluid flow rate, and sub...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260210910
更新日期:1979-09-01 00:00:00
abstract::A propanologenic (i.e., 1-propanol-producing) bacterium Escherichia coli strain was previously derived by activating the genomic sleeping beauty mutase (Sbm) operon. The activated Sbm pathway branches out of the tricarboxylic acid (TCA) cycle at the succinyl-CoA node to form propionyl-CoA and its derived metabolites o...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27276
更新日期:2020-05-01 00:00:00
abstract::Using phage display technology, we have isolated 12-mer peptide ligands that bind to human blood outgrowth endothelial cells (HBOEC). To avoid non-specific binding we apply negative-positive selection approach by pre-incubating the library with non-HBOEC. The selected phage clones bind to their target cell population ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21420
更新日期:2007-09-01 00:00:00
abstract::In view of a possible application of the alpha-amylase from Bacillus licheniformis as a time-temperature integrator for evaluation of heat processes,(11) thermal inactivation kinetics of the dissolved and covalently immobilized enzyme were studied in the temperature range 90-108 degrees C. The D-values (95 degrees C) ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260400309
更新日期:1992-07-01 00:00:00
abstract::Start-up phenomena in microbial biokinetic assays are not captured by the most commonly used growth-related equations. In this study we propose a new respirometric experimental design to estimate intrinsic growth parameters that allow us to avoid these limitations without data omission, separate mathematical treatment...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22518
更新日期:2010-01-01 00:00:00
abstract::Pretreatment methods were compared with steam explosion, and differing views on the relative importance of mechanical and chemical effects were outlined. Hydrolysis was desirable; pyrolysis was undesirable. The effects of initial moisture content on steam consumption, mechanism and rate of heat transfer, pentosan solu...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260290213
更新日期:1987-02-01 00:00:00