Microbial granulation for lactic acid production.

abstract：:The heterogeneous nature of hollow fiber reactors for cell cultivation requires special considerations for proper design and operation. Downstream concentration of high-molecular-weight proteins has been measured in the shell side of ultrafiltration hollow fiber bioreactors. This distribution resulted from shell-side ...

journal_title：Biotechnology and bioengineering

authors： Piret JM,Cooney CL

更新日期：1990-11-01 00:00:00

abstract：:In this study, the inhibitory effects and anaerobic biotransformation of acrylic acid in computer-controlled pH-stat completely stirred tank reactors (CSTRs) with two different cultures, namely unacclimated and acrylate-acclimated acetate-enriched Methanosarcina and homogenized (crushed) granular cultures, were invest...

journal_title：Biotechnology and bioengineering

authors： Demirer GN,Speece RE

更新日期：1999-01-20 00:00:00

abstract：:Many microorganisms are capable of sequestering and concentrating heavy metals from their aqueous environment. While much research has beep carried out on the uptake of single species of metal ions, little attention seems to have been given to the study of multimetal ion systems. A mathematical model has previously be...

journal_title：Biotechnology and bioengineering

authors： Ting YP,Prince IG,Lawson F

更新日期：1991-03-05 00:00:00

abstract：:Liver tissue engineering aims to create transplantable liver grafts that can serve as substitutes for donor's livers. One major challenge in creating a fully functional liver tissue has been to recreate the biliary drainage in an engineered liver construct through integration of bile canaliculi (BC) with the biliary d...

journal_title：Biotechnology and bioengineering

authors： Hafiz EOA,Bulutoglu B,Mansy SS,Chen Y,Abu-Taleb H,Soliman SAM,El-Hindawi AAF,Yarmush ML,Uygun BE

更新日期：2021-01-01 00:00:00

abstract：:The glycerol fed-batch fermentation by Klebsiella pneumoniae CGMCC 1.6366 exhibited the sequential synthesis of products, including acetate, 1,3-propanediol (1,3-PD), 2,3-butanediol, ethanol, succinate, and lactate. The dominant flux distribution was shifted from acetate formation to 1,3-PD formation in early- exponen...

journal_title：Biotechnology and bioengineering

authors： Zheng ZM,Xu YZ,Liu HJ,Guo NN,Cai ZZ,Liu DH

更新日期：2008-08-01 00:00:00

abstract：:A detailed mechanistic model of enzymatic cellulose hydrolysis has been developed. The behavior of individual cellulase enzymes and parameters describing the cellulose surface properties are included. Results obtained for individual enzymes (T. reesei endoglucanase 2 and cellobiohydrolase I) and systems with both enzy...

journal_title：Biotechnology and bioengineering

authors： Levine SE,Fox JM,Blanch HW,Clark DS

更新日期：2010-09-01 00:00:00

abstract：:We originally investigated the suitability of chitosan scaffolds loaded with bone morphogenetic protein 6 (BMP-6) in both stationary and dynamic conditions for cartilage tissue engineering. In the first part of the present study, ATDC5 murine chondrogenic cells were seeded in chitosan and BMP-6 loaded chitosan scaffol...

journal_title：Biotechnology and bioengineering

authors： Tiğli RS,Gümüşderelioğlu M

更新日期：2009-10-15 00:00:00

abstract：:Glycerol is considered as an ideal feedstock for producing bioplastics via bacterial fermentation due to its ubiquity, low price, and high degree of reduction substrate. In this work, we study the yield and cause of limitation in poly(3-hydroxybutyrate) (PHB) production from glycerol. Compared to glucose-based PHB pro...

journal_title：Biotechnology and bioengineering

authors： Tanadchangsaeng N,Yu J

更新日期：2012-11-01 00:00:00

abstract：:Linear relations among net conversion rates of primary metabolites in fermentation processes can be determined by using the macroscopic or pathway balancing methods. It is shown that the process observability can be improved by incorporating both the abiotic proton balance and the pH control reagent addition rate meas...

journal_title：Biotechnology and bioengineering

authors： Siano SA

更新日期：1996-12-20 00:00:00

abstract：:Dilute aqueous solutions of polymers released by marine phytoplankton (microalgae) were shown to effectively reduce drag in capillary pipe flow. Tests were performed in a capillary turbulent flow viscometer which extruded small samples under high pressures. In all, 22 species were screened, and the products of one chl...

journal_title：Biotechnology and bioengineering

authors： Ramus J,Kenney BE,Shaughnessy EJ

更新日期：1989-01-25 00:00:00

abstract：:Water permeability (Lp), calculated from the volume variations of cells subjected to an osmotic shock, is classically used to characterize cell membrane properties. In this work, we have shown the importance of the kind of mixing reactor used to measure the Lp parameter. A mathematical model including the mixing time ...

journal_title：Biotechnology and bioengineering

authors： de Marañón IM,Gervais P,Molin P

更新日期：1997-10-05 00:00:00

abstract：:Three cell lines, that is, the human breast cancer cell line (MCF-7) and the human mammary epithelial cell line (S-1) and its malignant form (T4-2) were embedded in a reconstituted basement membrane (Matrigel) that had 20-nL pyramid-shaped silicon microstructures. The proliferative behavior of the MCF-7 cells was depe...

journal_title：Biotechnology and bioengineering

authors： Torisawa YS,Nashimoto Y,Yasukawa T,Shiku H,Matsue T

更新日期：2007-06-15 00:00:00

abstract：:The results from this study demonstrate that neutral resins can selectively concentrate specific indole alkaloids from dilute aqueous mixtures. Adsorption was observed to provide a one to two order of magnitude improvement in concentrating these alkaloids, as compared to an equivalent single-staged extraction. Since t...

journal_title：Biotechnology and bioengineering

authors： Payne GF,Shuler ML

更新日期：1988-06-05 00:00:00

abstract：:Remarkable increases in enzyme catalytic stability resulting from addition of charged water-soluble polymers have recently been reported, suggesting that use of these polymers may be an attractive general strategy for enzyme stabilization. To test the proposed hypothesis that coulombic forces between water-soluble pol...

journal_title：Biotechnology and bioengineering

authors： Foreman TM,Khalil M,Meier P,Brainard JR,Vanderberg LA,Sauer NN

更新日期：2001-11-01 00:00:00

abstract：:Herein, we report the development of a microbial bioprocess for high-level production of 5-aminolevulinic acid (5-ALA), a valuable non-proteinogenic amino acid with multiple applications in medical, agricultural, and food industries, using Escherichia coli as a cell factory. We first implemented the Shemin (i.e., C4) ...

journal_title：Biotechnology and bioengineering

authors： Miscevic D,Mao JY,Kefale T,Abedi D,Moo-Young M,Perry Chou C

更新日期：2021-01-01 00:00:00

abstract：:Thermodynamic feasibility analysis (TFA) has been used as a tool capable of providing additional constraints to the mass balance-based methods of analysis of metabolic networks (e.g., flux balance analysis). Several publications have recently appeared in which TFA of different metabolic pathways from relatively simple...

journal_title：Biotechnology and bioengineering

authors： Vojinović V,von Stockar U

更新日期：2009-07-01 00:00:00

abstract：:Blood vessel development is critical for the continued growth and progression of solid tumors and, therefore, makes an attractive target for improving cancer therapy. Indeed, vascular-targeted therapies have been extensively explored but they have shown minimal efficacy as monotherapies. Combretastatin A4 (CA-4) is a ...

journal_title：Biotechnology and bioengineering

authors： Liu Y,Kim YJ,Siriwon N,Rohrs JA,Yu Z,Wanga P

更新日期：2018-06-01 00:00:00

abstract：:Temperature shifting was investigated as a means of improving cloned-gene product yields form a recombinant Escherichia coli containing the temperature-sensitive plasmid, pOU 140. In a series of shaker flask fermentations recombinant cells were thermally induced for different time periods. The growth, stability, and p...

journal_title：Biotechnology and bioengineering

authors： Dipasquantonio VM,Betenbaugh MJ,Dhurjati P

更新日期：1987-03-01 00:00:00

abstract：:Hydrogen production by Anabaena variabilis ATCC 29413 and of its mutant PK84, grown in batch cultures, was studied in a photobioreactor. The highest volumetric H(2) production rates of native and mutant strains were found in cultures grown at gradually increased irradiation. The native strain evolved H(2) only under a...

journal_title：Biotechnology and bioengineering

authors： Tsygankov AA,Borodin VB,Rao KK,Hall DO

更新日期：1999-09-20 00:00:00

abstract：:Downstream processing operations are often carried out blind in the process timescale since product monitoring on-line is not common. Knowledge of the location and concentration of the product and key contaminants is complementary to other process information for process development and, if available on-line in conjun...

journal_title：Biotechnology and bioengineering

authors： Holwill IJ,Chard SJ,Flanagan MT,Hoare M

更新日期：1997-01-05 00:00:00

abstract：:A semiequilibrium model of chromatography has been developed to account for the elution profile of high concentration bands. The use of this model requires the prior determination of the equilibrium isotherms of the studied compounds in the chromatographic phase system. The model is applied here to a study of the isoc...

journal_title：Biotechnology and bioengineering

authors： Katti A,Huang JX,Guiochon G

更新日期：1990-07-01 00:00:00

abstract：:Bioprocesses operated in batch mode can induce adverse effects like overflow metabolism, substrate inhibition, osmotic inhibition, oxygen limitation, and catabolite repression. To avoid these adverse effects, fed-batch is the predominant operation mode in industrial production. Nevertheless, screening for optimal prod...

journal_title：Biotechnology and bioengineering

authors： Habicher T,Czotscher V,Klein T,Daub A,Keil T,Büchs J

更新日期：2019-09-01 00:00:00

abstract：:A biodevice involving thiolated ssDNA and engineered cytochrome b5 linked through a cis-platine bridge is described. This original nanostructure is associated to a supported membrane through a floating anchor, thus constituting a dynamic bidimensionnal DNA capture device contrasting with the constrained geometry of cu...

journal_title：Biotechnology and bioengineering

authors： Boireau W,Bombard S,Sari MA,Pompon D

更新日期：2002-01-20 00:00:00

abstract：:In this study, we assessed the importance of cytoskeleton organization in the mammalian cells used to produce therapeutic proteins. Two cytoskeletal genes, Actin alpha cardiac muscle 1 (ACTC1) and a guanosine triphosphate GTPase-activating protein (TAGAP), were found to be upregulated in highly productive therapeutic ...

journal_title：Biotechnology and bioengineering

authors： Pourcel L,Buron F,Arib G,Le Fourn V,Regamey A,Bodenmann I,Girod PA,Mermod N

更新日期：2020-04-01 00:00:00

abstract：:Kluyveromyces marxianus UCD (FST) 55-82 cells were immobilized in Na alginate beads and used in a packed-bed bioreactor system for the continuous production of ethanol from the extract of Jerusalem artichoke tubers. Volumetric ethanol productivities of 104 and 80 g ethanol/ L/h were obtained at 80 and 92% sugar utiliz...

journal_title：Biotechnology and bioengineering

authors： Margaritis A,Bajpai P

更新日期：1982-07-01 00:00:00

abstract：:The study reports on the development of a bioreactor for the production of alpha-keto acids from D,L- or D-amino acids using Rhodotorula gracilis D-amino acid oxidase. D-amino acid oxidase was co-immobilized with catalase on Affi-Gel 10 matrix, and the reactor was operated as a continuous-stirred tank reactor (CSTR) o...

journal_title：Biotechnology and bioengineering

authors： Butò S,Pollegioni L,D'Angiuro L,Pilone MS

更新日期：1994-12-01 00:00:00

abstract：:Many enzymes or fluorescent proteins produced in Escherichia coli are enzymatically active or fluorescent respectively when deposited as inclusion bodies. The occurrence of insoluble but functional protein species with native-like secondary structure indicates that solubility and conformational quality of recombinant ...

journal_title：Biotechnology and bioengineering

authors： Martínez-Alonso M,García-Fruitós E,Villaverde A

更新日期：2008-12-15 00:00:00

abstract：:Hydrocarbon oils of the alga Botryococcus braunii, extracted from a natural "bloom" of the plant, have been hydrocracked to produce a distillate comprising 67% gasoline fraction, 15% aviation turbine fuel fraction, 15% diesel fuel fraction, and 3% residual oil. The distillate was examined by a number of standard petro...

journal_title：Biotechnology and bioengineering

authors： Hillen LW,Pollard G,Wake LV,White N

更新日期：1982-01-01 00:00:00

abstract：:Various structure-similar plant secondary metabolites like ginseng saponins (ginsenosides) possess different or even totally opposite biological activities. Intentional manipulation of the ginsenoside heterogeneity in cellular biosynthesis is of great interest and significance [Zhong and Yue (2005); Adv Biochem Eng Bi...

journal_title：Biotechnology and bioengineering

authors： Yue CJ,Zhou X,Zhong JJ

更新日期：2008-08-01 00:00:00

abstract：:This article describes the synthesis and regulation of beta(1-3)glucanase and protease enzymes from the cell lytic system of Oerskovia xanthineolytica LL-G109 in continuous culture using different concentrations of carbon source (glucose) and inducer (glucan). These two enzyme activities are the main components of a l...

journal_title：Biotechnology and bioengineering

authors： Andrews BA,Asenjo JA

更新日期：1987-10-05 00:00:00