Abstract:
:This article describes a methodology that implements a Markov decision process (MDP) optimization technique in a real time fed-batch experiment. Biological systems can be better modeled under the stochastic framework and MDP is shown to be a suitable technique for their optimization. A nonlinear input/output model is used to calculate the probability transitions. All elements of the MDP are identified according to physical parameters. Finally, this study compares the results obtained when optimizing ethanol production using the infinite horizon problem, with total expected discount policy, to previous experimental results aimed at optimizing ethanol production using a recombinant Escherichia coli fed-batch cultivation. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55: 317-327, 1997.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Saucedo VM,Karim MNdoi
10.1002/(SICI)1097-0290(19970720)55:2<317::AID-BITsubject
Has Abstractpub_date
1997-07-20 00:00:00pages
317-27issue
2eissn
0006-3592issn
1097-0290journal_volume
55pub_type
杂志文章abstract::A model for the prediction of protein partition coefficients in aqueous two-phase systems has been developed. This model accounts for both charge-independent and electrostatic effects. The determination of nonelectrostatic effects was based on the model of Eiteman and Gainer for uncharged solutes while the electrostat...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19971105)56:3<248::AID-BIT
更新日期:1997-11-05 00:00:00
abstract::The emissions of nitrous oxide (N(2)O) and nitric oxide (NO) from biological nitrogen removal (BNR) operations via nitrification and denitrification is gaining increased prominence. While many factors relevant to the operation of denitrifying reactors can influence N(2)O and NO emissions from them, the role of differe...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22704
更新日期:2010-06-15 00:00:00
abstract::James (Jay) E. Bailey was a pioneer in biotechnology and biochemical engineering. During his 30 years in academia he made seminal contributions to many fields of chemical engineering science, including catalysis and reaction engineering, bioprocess engineering, mathematical modeling of cellular processes, recombinant ...
journal_title:Biotechnology and bioengineering
pub_type: 传,历史文章,杂志文章
doi:10.1002/bit.10404
更新日期:2002-09-05 00:00:00
abstract::Protein glycosylation, or the attachment of sugar moieties (glycans) to proteins, is important for protein stability, activity, and immunogenicity. However, understanding the roles and regulations of site-specific glycosylation events remains a significant challenge due to several technological limitations. These limi...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26502
更新日期:2018-03-01 00:00:00
abstract::Real-time polymerase chain reaction (PCR) is one of the most sensitive and accurate methods for quantifying transcript levels especially for those expressed at low abundance. The selective amplification of target DNA over multiple cycles allows its initial concentration to be determined. The amplification rate is a co...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20555
更新日期:2005-09-30 00:00:00
abstract::The bacterial consortium MPD-M, isolated from sediment associated with Colombian mangrove roots, was effective in the treatment of hydrocarbons in water with salinities varying from 0 to 180 g L(-1). Where the salinity of the culture medium surpassed 20 g L(-1), its effectiveness increased when the cells were immobili...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10318
更新日期:2002-07-20 00:00:00
abstract::A balance between proliferation and cell death is critical for achieving desirable high cell densities in mammalian cell culture. In this study, we evaluate a recently discovered anti-apoptotic gene, aven, and examine its effectiveness alone and in combination with a member of the Bcl-2 family, bcl-xL. The commerciall...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10913
更新日期:2004-03-20 00:00:00
abstract::Pulses of several nonreacting solutes were fed to a column containing immobilized enzyme catalyst beads. The response curves were recorded, and their first and second moments were determined by numerical integration. From the moment analysis, the void volume, internal porosity, and tortuosity of the support were calcu...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260250707
更新日期:1983-07-01 00:00:00
abstract::A new high throughput technique for enzyme characterization with specific attention to the long term stability, called "Enzyme Test Bench," is presented. The concept of the Enzyme Test Bench consists of short term enzyme tests in 96-well microtiter plates under partly extreme conditions to predict the enzyme long term...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22242
更新日期:2009-06-01 00:00:00
abstract::In this study, an analysis of the transition between the inactive ("closed") and active ("open") conformations of Candida rugosa lipase in solution is performed using irreversible enzyme inhibitors, cyclic saligenin phosphates. It is shown that >90% inhibition of the enzyme activity toward water-soluble substrates (es...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/1097-0290(20010105)72:1<108::aid-bit14>3.0
更新日期:2001-01-05 00:00:00
abstract::A gene coding for human parathyroid hormone (hPTH) was synthesized and cloned into a yeast expression and secretion vector containing the mating factor alpha pre-pro leader sequence and the galactose-inducible promoter, GAL10. The intact hPTH(1-84) was found to be secreted into the culture medium. As observed in the p...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19980120)57:2<245::aid-bit
更新日期:1998-01-20 00:00:00
abstract::To investigate the effects of factors secreted by different cell lines on human monoclonal antibody (MAb) integrity, 600 mg of a human MAb, which specifically binds to human erythrocytes, were produced in a perfusion process. After purification by protein A affinity chromatography, the MAb was used for integrity testi...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260450202
更新日期:1995-01-20 00:00:00
abstract::Trichoplusia ni larvae were infected with baculoviruses containing genes for the expression of ultraviolet optimized green fluorescent protein (GFPuv) and several product proteins. A GFP-specific optical probe was used to both excite the green fluorescent protein (lambda(ex) = 385 nm), and subsequently monitor fluores...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10668
更新日期:2003-07-20 00:00:00
abstract::Proteinaceous organic wastes are suitable substrates to produce high added-value products in anaerobic mixed-culture fermentations. In these processes, the stoichiometry of the biotransformation depends highly on operational conditions such as pH or feeding characteristics and there are still no tools that allow the p...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27177
更新日期:2020-01-01 00:00:00
abstract::A spinning cylindrical filter is often used to retain mammalian cells in a continuous perfusion bioreactor. This device, known as a spinfilter, has typically been with pore size smaller than the cell particles (single cells or aggregates) in order to achieve cell separation. For single cells in suspension, such an ope...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260400809
更新日期:1992-10-20 00:00:00
abstract::The removal of N-compounds in the sequencing batch airlift reactor (SBAR) containing granular sludge was studied under conditions of decreased dissolved oxygen (DO). A simulation model was developed to describe and evaluate the effects of several process conditions in the SBAR on N-removal performance. The model descr...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.1167
更新日期:2001-10-05 00:00:00
abstract::The suitability of using annually grown, carrot-sized buffalo gourd (Cucurbita foetidissima) roots as a feedstock for alcoholic fermentation was explored. Roots grown in 1982 and 1983 were slurried, dextrinized and saccharified using Takathermtrade mark and Diazymetrade mark (commercial enzymes manufactured by Miles L...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260290406
更新日期:1987-03-01 00:00:00
abstract::The secretion of heterologous IgG proteins in the baculovirus-insect cell expression system is accompanied by substantial insoluble immunoglobulin in the infected cells. The accumulation of these insoluble forms suggests a limitation in the processing and secretory pathway of the infected cells. As a result, cytosolic...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:1998-04-20 00:00:00
abstract::A sequencing batch membrane biofilm reactor (SBMBfR) was developed for simultaneous carbon, nitrogen, and phosphorus removal from wastewater. This reactor was composed of two functional parts: (1) a gas-permeable membrane on which a nitrifying biofilm formed and (2) a bulk solution in which bacteria, mainly denitrifyi...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20887
更新日期:2006-07-05 00:00:00
abstract::Metastatic disease remains one of the primary reasons for cancer-related deaths, yet the majority of in vitro cancer models focus on the primary tumor sites. Here, we describe a metastasis-on-a-chip device that houses multiple bioengineered three-dimensional (3D) organoids, established by a 3D photopatterning techniqu...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26871
更新日期:2019-04-01 00:00:00
abstract::Catalytic membranes, obtained by immobilizing thermophilic beta-glycosidase onto nylon supports, were used in a nonisothermal bioreactor to study the effect of temperature gradients on the rate of enzyme reaction. Two experimental approaches were carried out to explain the molecular mechanisms by which the temperature...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:1998-07-05 00:00:00
abstract::The bioartificial pancreas encapsulating pancreatic islets in immunoprotective hydrogel is a promising therapy for Type 1 diabetes. As pancreatic islets are highly metabolically active and exquisitely sensitive to hypoxia, maintaining O2 supply after transplantation remains a major challenge. In this study, we address...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26913
更新日期:2019-05-01 00:00:00
abstract::C-terminal lysine (C-K) variants are commonly observed in therapeutic monoclonal antibodies and recombinant proteins. Heterogeneity of C-K residues is believed to result from varying degree of proteolysis by endogenous carboxypeptidase(s) during cell culture production. The achievement of batch-to-batch culture perfor...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24510
更新日期:2012-09-01 00:00:00
abstract::In order to carry out an effective enzyme reaction, the preparation of soluble-insoluble immobilized enzyme was investigated. Proteases were selected as model enzymes, and their immobilization was carried out by using an enteric coating polymer as a carrier. Among the polymers tested, methacrylic acid-methylacrylate-m...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260290612
更新日期:1987-04-01 00:00:00
abstract::Bacterial profile modification (BPM), a form of tertiary oil recovery, diverts water from the water-flooded high-permeability zone into the oil-bearing low-permeability zone. During field use, exopolymer-producing bacteria plug the high-permeability zone only in the immediate vicinity of the injection point (the near-...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20315
更新日期:2005-01-05 00:00:00
abstract::The sec-alcohol dehydrogenase activity of whole cells of Rhodococcus ruber DSM 44541 has been employed as an efficient biocatalytic redox system due to the use of acetone and 2-propanol at elevated concentrations for cofactor regeneration in the oxidation and reduction mode, respectively, and external addition of NADH...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10540
更新日期:2003-03-30 00:00:00
abstract::The biosynthesis of invertase by Saccharomyces carlsbergensis LAM 1068 was studied in relation to its glucose effect at both unsteady and steady states of growth. Experimental correlations between the dilution rate and invertase specific activity (E/X) in chemostat cultures led to an optimum for the enzyme synthesis a...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260180807
更新日期:1976-08-01 00:00:00
abstract::A novel deuterium ((2)H) NMR technique as developed for measuring the total number of deuterons exchanged by lyophilised protein samples following hydrogen-deuterium (H-D) exchange. Using this methodology differences in the H-D exchange behaviour of the proteolytic enzyme subtilisin Carlsberg hydrated either in air or...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:2000-11-05 00:00:00
abstract::A bubble column (0.05 m(3)) and an air-lift fermentor (1.2 m(3)) were used for the production of the exocellular microbial polysaccharide xanthan with Xanthomonas campestris in a synthetic medium. Upon oxygen depletion in the liquid, the xanthan production rate dropped sharply and then became a linear function of the ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260390113
更新日期:1992-01-05 00:00:00
abstract::The sensitivity of a conceptual model of a foam emulsion bioreactor (FEBR) used for the control of toluene vapors in air was examined. Model parametric sensitivity studies showed which parameters affect the removal of toluene (as model pollutant) in the FEBR the most significantly, and enabled definition of the limits...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22122
更新日期:2009-02-15 00:00:00