Xanthan production in bubble column and air-lift reactors.

abstract：:Cancer is the leading cause of death in industrialized countries. Cancer therapy often involves monoclonal antibodies or small-molecule drugs, but carbohydrate-binding lectins such as mistletoe (Viscum album) viscumin offer a potential alternative treatment strategy. Viscumin is toxic in mammalian cells, ruling them o...

journal_title：Biotechnology and bioengineering

authors： Gengenbach BB,Keil LL,Opdensteinen P,Müschen CR,Melmer G,Lentzen H,Bührmann J,Buyel JF

更新日期：2019-09-01 00:00:00

abstract：:Escherichia coli and Desulfovibrio desulfuricans reduce Tc(VII) (TcO(4)(-)) with formate or hydrogen as electron donors. The reaction is catalyzed by the hydrogenase component of the formate hydrogenlyase complex (FHL) of E. coli and is associated with a periplasmic hydrogenase activity in D. desulfuricans. Tc(VII) re...

journal_title：Biotechnology and bioengineering

authors： Lloyd JR,Thomas GH,Finlay JA,Cole JA,Macaskie LE

更新日期：1999-01-01 00:00:00

abstract：:We describe a method by which the degree of bubble saturation can be determined by measuring the velocity of single bubbles at different heights from the bubble source in pure water containing increasing concentrations of surfactants. The highest rising velocities were measured in pure water. Addition of surfactants c...

journal_title：Biotechnology and bioengineering

authors： Jordan M,Eppenberger HM,Sucker H,Widmer F,Einsele A

更新日期：1994-03-15 00:00:00

abstract：:Tumor-targeted Salmonella VNP20009 preferentially replicate within tumor tissue and partially suppress tumor growth in murine tumor models. These Salmonella have the ability to locally induce apoptosis when they are in direct contact with cancer cells but they lack significant bystander killing, which may correlate wi...

journal_title：Biotechnology and bioengineering

authors： Quintero D,Carrafa J,Vincent L,Bermudes D

更新日期：2016-12-01 00:00:00

abstract：:L-Threonine, a kind of essential amino acid, has numerous applications in food, pharmaceutical, and aquaculture industries. Fermentative l-threonine production from glucose has been achieved in Escherichia coli. However, there are still several limiting factors hindering further improvement of l-threonine productivity...

journal_title：Biotechnology and bioengineering

authors： Liu J,Li H,Xiong H,Xie X,Chen N,Zhao G,Caiyin Q,Zhu H,Qiao J

更新日期：2019-01-01 00:00:00

abstract：:In vivo mesenchymal stem cell (MSC) survival is relevant to therapeutic applications requiring engraftment and potentially to nonengraftment applications as well. MSCs are a mixture of progenitors at different stages of cellular aging, but the contribution of this heterogeneity to the survival of MSC implants is unkno...

journal_title：Biotechnology and bioengineering

authors： Madsen SD,Jones SH,Tucker HA,Giler MK,Muller DC,Discher CT,Russell KC,Dobek GL,Sammarco MC,Bunnell BA,O'Connor KC

更新日期：2020-01-01 00:00:00

abstract：:Fibrous proteins, such as silk, elastin and collagen are finding broad impact in biomaterial systems for a range of biomedical and industrial applications. Some of the key advantages of biosynthetic fibrous proteins compared to synthetic polymers include the tailorability of sequence, protein size, degradation pattern...

journal_title：Biotechnology and bioengineering

authors： Yigit S,Dinjaski N,Kaplan DL

更新日期：2016-05-01 00:00:00

abstract：:Screening tests of different marine algae biomas types revealed a high passive biosorptive uptake of lead up to 270 mg Pb/g of biomass in some brown marine algae. Members of the order Fucales perfomed particularly well in this descending sequence: Fucus > Ascophyllum > Sargassum. Although decreasing the swelling of we...

journal_title：Biotechnology and bioengineering

authors： Holan ZR,Volesky B

更新日期：1994-05-01 00:00:00

abstract：:Olive oil hydrolysis using Chromobacterium viscosum lipase B in a reversed micellar media was investigated in a membrane reactor. The dynamic evolution of the product concentration both in the concentrate and permeate stream was analyzed using a mechanistic model previously developed by us and further modified in this...

journal_title：Biotechnology and bioengineering

authors： Prazeres DM,Lemos F,Garcia FA,Cabral JM

更新日期：1993-09-05 00:00:00

abstract：:Utilising whole cells of recombinant Escherichia coli K27 (pCYP102, pGEc47) containing active cytochrome P450BM-3 monooxygenase [E.C. 1. 14.14.1], multiple oxidations of saturated and unsaturated fatty acids were performed by the enzyme under conditions of excess oxygen. The amount of oxygen dissolved in the culture m...

journal_title：Biotechnology and bioengineering

authors： Schneider S,Wubbolts MG,Oesterhelt G,Sanglard D,Witholt B

更新日期：1999-08-05 00:00:00

abstract：:The G-H loop of foot-and-mouth disease virus is a disordered protrusion of the VP1 protein exposed on the virion surface. This short stretch includes an arginine-glycine-aspartic acid tripeptide, a recognized integrin-binding motif, which is responsible for cell attachment and infection. Eight copies of a peptide repr...

journal_title：Biotechnology and bioengineering

authors： Villaverde A,Feliu JX,Arís A,Harbottle RP,Benito A,Coutelle C

更新日期：1998-08-05 00:00:00

abstract：:We report the isolation of mutant strains of the methylotrophic yeast Hansenula polymorpha that are able to efficiently oxidize ethanol to acetaldehyde in an intact cell system. The oxidation reaction is catalyzed by alcohol oxidase (AOX), a key enzyme in the methanol metabolic pathway that is typically present only i...

journal_title：Biotechnology and bioengineering

authors： Moroz OM,Gonchar MV,Sibirny AA

更新日期：2000-04-05 00:00:00

abstract：:The activities of pyruvate kinase (PK), pyruvate: formate-lyase (PFL), pyruvate dehydrogenase (PDH), and citrate synthase (CS) involved in the anaerobic glycerol conversion by Klebsiella pneumoniae were studied in continuous culture under conditions of steady states and sustained oscillations. Both the in vitro and in...

journal_title：Biotechnology and bioengineering

authors： Menzel K,Ahrens K,Zeng A,Deckwer W

更新日期：1998-12-05 00:00:00

abstract：:Mass transfer is known to play a critical role in bioprocess performance and henceforth monitoring dissolved O2 (DO) and dissolved CO2 (dCO2 ) is of paramount importance. At bioreactor level these parameters can be monitored online and can be controlled by sparging air/oxygen or stirrer speed. However, traditional sma...

journal_title：Biotechnology and bioengineering

authors： Chopda VR,Holzberg T,Ge X,Folio B,Wong L,Tolosa M,Kostov Y,Tolosa L,Rao G

更新日期：2020-04-01 00:00:00

abstract：:As cartilage is incapable of self-healing upon severe degeneration because of the lack of blood vessels, cartilage tissue engineering is gaining importance in the treatment of cartilage defects. This study was designed to improve cartilage tissue regeneration by expressing tissue transglutaminase variant 2 (TGM2_v2) i...

journal_title：Biotechnology and bioengineering

authors： Calikoglu Koyuncu AC,Nayman AH,Telci D,Torun Kose G

更新日期：2020-06-01 00:00:00

abstract：:The diffusion characteristics of sucrose, a nutrient, and yohimbine, a secondary metabolite, in alginate gel beads, with or without entrapped periwinkle (Catharanthus roseus) or apple (Malus domestica) cells, were investigated. Effective diffusivities of both solutes in the gel beads were determined by two different m...

journal_title：Biotechnology and bioengineering

authors： Pu HT,Yang RY

更新日期：1988-09-20 00:00:00

abstract：:For the application of immobilized enzymes, fixed bed reactors are used almost exclusively. Fixed bed reactors have specific disadvantages, especially for processes with a deactivating catalyst. Therefore, we have studied a novel reactor type with continuous transport of the immobilized biocatalyst. Flow of biocatalys...

journal_title：Biotechnology and bioengineering

authors： Vos HJ,Groen DJ,Potters JJ,Luyben KC

更新日期：1990-08-05 00:00:00

abstract：:Cellulase (Cellulosin AC-8) was immobilized on poly-L-glutamic acid. This immobilized cellulase (IC) is water soluble in the neutral and alkaline solutions, where IC has the activity, while IC can be made insoluble by lowering the pH so that it can be recovered from the reaction mixture with its activity. The optimum ...

journal_title：Biotechnology and bioengineering

authors： Takeuchi T,Makino K

更新日期：1987-02-01 00:00:00

abstract：:This work investigated the formation of microbial granules to boost the productivity of lactic acid (LA). The flocculated form of LA-producing microbial consortium, dominated by Lactobacillus sp. (91.5% of total sequence), was initially obtained in a continuous stirred-tank reactor (CSTR), which was fed with 2% glucos...

journal_title：Biotechnology and bioengineering

authors： Kim DH,Lee MK,Hwang Y,Im WT,Yun YM,Park C,Kim MS

更新日期：2016-01-01 00:00:00

abstract：:The design of new generation bioprocessing plants is increasingly dependent on the design of process-compatible microorganisms. The latter, whether through genetic or physiological manipulations, can be greatly assisted by metabolic engineering. An emerging powerful tool in metabolic engineering research is computer-a...

journal_title：Biotechnology and bioengineering

authors： Dean JP,Dervakos GA

更新日期：1998-04-05 00:00:00

abstract：:Increased stability at alkaline pH should be a valuable attribute for the utilization of penicillin acylase in bioreactors employed to convert penicillins into 6-aminopenicillanic acid, a precursor of semisynthetic penicillins. In these systems, base is added for pH control, which results in local alkaline conditions ...

journal_title：Biotechnology and bioengineering

authors： Del Río G,Rodríguez ME,Munguía ME,López-Munguí A,Soberón X

更新日期：1995-10-20 00:00:00

abstract：:The hydrolysis of lactose by a beta-galactosidase from the thermophilic microorganism Thermus sp. strain T2, both in solution and immobilized on a commercial silica-alumina, has been studied. The enzyme has been previously produced by Escherichia coli JM101 harboring the plasmid pBGT1, which contains the codifying gen...

journal_title：Biotechnology and bioengineering

authors： Ladero M,Perez MT,Santos A,Garcia-Ochoa F

更新日期：2003-01-20 00:00:00

abstract：:The evolution of microbial populations involved in simulated-heap leaching of a polymetallic black schist sulfide ore (from the recently-commissioned Talvivaara mine, Finland) was monitored in aerated packed bed column reactors over a period of 40 weeks. The influence of ore particle size (2-6.5 mm and 6.5-12 mm) on c...

journal_title：Biotechnology and bioengineering

authors： Wakeman K,Auvinen H,Johnson DB

更新日期：2008-11-01 00:00:00

abstract：:Escherichia coli cells with formate hydrogenlyase activity that were immobilized in agar beads produced hydrogen from glucose at the approximate yield of 0.6 mole of hydrogen per mole. Succinate or thiosulphate added to glucose increased hydrogen production two-fold. Thiosulphate did not increase the rate of hydrogen ...

journal_title：Biotechnology and bioengineering

authors： Nandi R,Dey S,Sengupta S

更新日期：2001-11-20 00:00:00

abstract：:Non-autologous somatic gene therapy is an alternate approach to delivering recombinant gene products through implantation of a "universal" donor cell line engineered to produce a therapeutic gene product. The cells are immunologically isolated by enclosure in immunoprotective microcapsules fabricated from alginate-pol...

journal_title：Biotechnology and bioengineering

authors： Awrey DE,Tse M,Hortelano G,Chang PL

更新日期：1996-11-20 00:00:00

abstract：:Protein aggregation of monoclonal antibodies (mAbs) is a common phenomenon associated with the production of these biopharmaceuticals. These aggregates can lead to adverse side effects in patients upon administration, thus expensive downstream processing steps to remove the higher molecular weight species are inevitab...

journal_title：Biotechnology and bioengineering

authors： Paul AJ,Handrick R,Ebert S,Hesse F

更新日期：2018-05-01 00:00:00

abstract：:Adenoviruses are human pathogens increasingly used as gene therapy and vaccination vectors. However, their impact on cell metabolism is poorly characterized. We performed carbon labeling experiments with [1,2-13 C]glucose or [U-13 C]glutamine to evaluate metabolic alterations in the amniocyte-derived, E1-transformed 1...

journal_title：Biotechnology and bioengineering

authors： Carinhas N,Koshkin A,Pais DA,Alves PM,Teixeira AP

更新日期：2017-01-01 00:00:00

abstract：:Multiparameter flow cytometric techniques developed in our laboratories have been used for the "at-line" study of fed-batch bacterial fermentations. These fermentations were done at two scales, production (20 m(3)) and bench (5 x 10(-3) m(3)). In addition, at the bench scale, experiments were undertaken where the diff...

journal_title：Biotechnology and bioengineering

authors： Hewitt CJ,Nebe-Von Caron G,Axelsson B,McFarlane CM,Nienow AW

更新日期：2000-11-20 00:00:00

abstract：:The feasibility of producing ethanol in a continuous system from cellulose using Clostridirrrn thermocellum was investigated. This anaerobic and therniophilic bacterium was able to degrade cellulose directly into ethanol with acetic acid, hydrogen. and carbon dioxide as by-products of this fermentation. The fermentati...

journal_title：Biotechnology and bioengineering

authors： Zertuche L,Zall RR

更新日期：1982-01-01 00:00:00

abstract：:Mevalonate is a useful metabolite synthesized from three molecules of acetyl-CoA, consuming two molecules of NADPH. Escherichia coli ( E. coli) catabolizes glucose to acetyl-CoA via several routes, such as the Embden-Meyerhof-Parnas (EMP) and the oxidative pentose phosphate (oxPP) pathways. Although the oxPP pathway s...

journal_title：Biotechnology and bioengineering

authors： Kamata K,Toya Y,Shimizu H

更新日期：2019-05-01 00:00:00