Abstract:
:Adenoviruses are human pathogens increasingly used as gene therapy and vaccination vectors. However, their impact on cell metabolism is poorly characterized. We performed carbon labeling experiments with [1,2-13 C]glucose or [U-13 C]glutamine to evaluate metabolic alterations in the amniocyte-derived, E1-transformed 1G3 cell line during production of a human adenovirus type 5 vector (AdV5). Nonstationary 13 C-metabolic flux analysis revealed increased fluxes of glycolysis (17%) and markedly PPP (over fourfold) and cytosolic AcCoA formation (nearly twofold) following infection of growing cells. Interestingly, infection of growth-arrested cells increased overall carbon flow even more, including glutamine anaplerosis and TCA cycle activity (both over 1.5-fold), but was unable to stimulate the PPP and was associated with a steep drop in AdV5 replication (almost 80%). Our results underscore the importance of nucleic and fatty acid biosynthesis for adenovirus replication. Overall, we portray a metabolic blueprint of human adenovirus infection, highlighting similarities with other viruses and cancer, and suggest strategies to improve AdV5 production. Biotechnol. Bioeng. 2017;114: 195-207. © 2016 Wiley Periodicals, Inc.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Carinhas N,Koshkin A,Pais DA,Alves PM,Teixeira APdoi
10.1002/bit.26063subject
Has Abstractpub_date
2017-01-01 00:00:00pages
195-207issue
1eissn
0006-3592issn
1097-0290journal_volume
114pub_type
杂志文章abstract::The mixed-culture anaerobic conversion of lactose to organic acids in a bench-scale continuous-flow stirredtank fermentor is considered. The major acidogenic end-product distribution with respect to the dilution rate are presented. A Monod chemostat model is employed to describe a microbial growth, and the influence o...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260330516
更新日期:1989-01-25 00:00:00
abstract::A stoichiometric equation has been derived which describes the interrelations among the various products and biomass in fermentations of butyric acid bacteria. The derivation of the equation is based on an assumed ATP yield, two biological regularities, and the biochemistry of product formation of the fermentations. T...
journal_title:Biotechnology and bioengineering
pub_type: 传,古典文章,历史文章,杂志文章
doi:
更新日期:2000-03-20 00:00:00
abstract::The possibility of enhancing the biomass productivity of a continuous culture of Saccharomyces cerevisiae growing on a glucose-limited medium is addressed. An unstructured Monod-type model is first identified using steady-state data. The culture is subjected to step changes in dilution rate, and it is seen that the Mo...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260340603
更新日期:1989-09-01 00:00:00
abstract::We propose that transcript levels for some genes are affected by the bacterial cell division cycle and this may be an important factor to consider when designing synthetic bacterial genomes. To test this hypothesis, transcript levels of 58 genes in Escherichia coli B/r A were determined at five times during the cell d...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22098
更新日期:2009-02-15 00:00:00
abstract::Two instruments were developed to mechanize the handling of anaerobic microorganisms for microbial mutant isolation. The instruments automatically dispense liquid or agar medium to large and small 96-well platesand petridishes. Protocols were developed for inoculating different microorganisms, and calibration curves o...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260280905
更新日期:1986-09-01 00:00:00
abstract::The glycosylation patterns of recombinant therapeutic glycoproteins can be engineered by overexpression of glycosyltransferases in the host cells used for glycoprotein production. Most prior glycosylation engineering experiments have involved constitutive expression of cloned glycosyltransferases. Here we use tetracyc...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:1999-12-05 00:00:00
abstract::The role of computers in the monitoring and control of fermentation processes has increased steadfastly. The ultimate utility of the machines will not depend on the availability of online sensors but also on the availability of techniques that combine direct measurements, leading towards estimates of variable closely ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260240713
更新日期:1982-07-01 00:00:00
abstract::A bioremediation process to remove Co(2+) from aqueous solution is investigated in this study using a magnetic resonance imaging (MRI) protocol to rapidly obtain multiple 2D spatially resolved Co(2+) ion concentration maps. The MRI technique is described in detail and its ability to determine the evolution in both axi...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21647
更新日期:2008-03-01 00:00:00
abstract::This article studies the operation of a new process for the production of biopolymers (polyhydroxyalkanoates, PHAs) at different applied organic load rates (OLRs). The process is based on the aerobic enrichment of activated sludge to obtain mixed cultures able to store PHAs at high rates and yields. A mixture of aceti...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20683
更新日期:2006-01-05 00:00:00
abstract::An unadapted mixed methanogenic sludge transformed formaldehyde into methanol and formate. The methanol to formate ratio obtained was 1:1. Formaldehyde conversion proceeded without any lag phase, suggesting the constitutive character of the formaldehyde conversion enzymes involved. Because the rate of formaldehyde con...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10294
更新日期:2002-08-05 00:00:00
abstract::PER.C6 cells were cultivated for propagation of a replication-defective adenovirus vector in serum-free suspension bioreactors. Cellular metabolism during cell growth and adenovirus propagation was fully characterized using on-line and off-line methods. The energy metabolism was found to accelerate transiently after a...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20455
更新日期:2005-06-05 00:00:00
abstract::The evolution of microbial populations involved in simulated-heap leaching of a polymetallic black schist sulfide ore (from the recently-commissioned Talvivaara mine, Finland) was monitored in aerated packed bed column reactors over a period of 40 weeks. The influence of ore particle size (2-6.5 mm and 6.5-12 mm) on c...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21951
更新日期:2008-11-01 00:00:00
abstract::Invertase from Candida utilis was immobilized on porous cellulose beads by an ionic-quanidino bond. The immobilized invertase showed optimum activity between pH 4.0 and 5.4, while the free enzyme had a sharp optimum at pH 4.1. Both temperature profiles were fairly similar up to 55 degrees C. However, above this temper...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260190307
更新日期:1977-03-01 00:00:00
abstract::Being biosurfactants, rhamnolipids create severe foaming when produced in aerobic Pseudomonas aeruginosa fermentation. The necessary reduction of aeration causes oxygen limitation and restricts cell and product concentrations. In this study, we evaluate the new strategy of rhamnolipid production under denitrification ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/1097-0290(20010105)72:1<25::aid-bit4>3.0.c
更新日期:2001-01-05 00:00:00
abstract::While many pretreatments attempt to improve the enzymatic digestibility of biomass by removing lignin, this study shows that improving the surface area accessible to cellulase is a more important factor for achieving a high sugar yield. Here we compared the pretreatment of switchgrass by two methods, cellulose solvent...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22919
更新日期:2011-01-01 00:00:00
abstract::The treatment of large segmental bone defects remains a challenge as infection, delayed union, and nonunion are common postoperative complications. A three-dimensional printed bioresorbable and physiologically load-sustaining graft substitute was developed to mimic native bone tissue for segmental bone repair. Fabrica...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26780
更新日期:2018-09-01 00:00:00
abstract::The dissolved oxygen (DO) is an important variable in aerobic fermentations and affects the cell growth and product formation. Dissolved oxygen control is difficult in batch fermentations because of the time-varying conditions, time delays, and the probe dynamics. Modeling of the various patterns of biological activit...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19981005)60:1<1::aid-bit1>
更新日期:1998-10-05 00:00:00
abstract::This article provides the analysis on biomass yields and energetic yields of the oleaginous yeasts. The biomass yields of the oleaginous yeasts are consistently lower than nonoleaginous microorganisms, whereas their energetic yields are higher. Data inconsistencies of literature are explained by the variation of energ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260280117
更新日期:1986-01-01 00:00:00
abstract::A model was formulated to examine specific experimental data of growth and heterologous product formation with recombinant Saccharomyces cerevisiae while incorporating available literature. The model simulated dry cell weight, glucose, ethanol, dissolved oxygen, human Epidermal Growth Factor (hEGF) production, fractio...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260350405
更新日期:1990-02-20 00:00:00
abstract::Modifications are made to an earlier thermodynamic model (TEEM1) for prediction of maximum microbial yields from aerobic and anaerobic as well as heterotrophic and autotrophic growth. The revised model (TEEM2) corrects for lower yields found with aerobic oxidations of organic compounds where an oxygenase is involved a...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21250
更新日期:2007-06-01 00:00:00
abstract::A new high throughput technique for enzyme characterization with specific attention to the long term stability, called "Enzyme Test Bench," is presented. The concept of the Enzyme Test Bench consists of short term enzyme tests in 96-well microtiter plates under partly extreme conditions to predict the enzyme long term...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22242
更新日期:2009-06-01 00:00:00
abstract::High hydrostatic pressure (HHP) stabilized glucose oxidase (GOx) against thermal inactivation. The apparent first-order kinetics of inactivation of GOx were investigated at 0.1-300 MPa and 58.8-80.0°C. At 240 MPa and 74.5°C, GOx inactivated at a rate 50 times slower than at atmospheric pressure at the same temperature...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26185
更新日期:2017-03-01 00:00:00
abstract::Oxygenases catalyze, among other interesting reactions, highly selective hydrocarbon oxyfunctionalizations, which are important in industrial organic synthesis but difficult to achieve by chemical means. Many enzymatic oxygenations have been described, but few of these have been scaled up to industrial scales, due to ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10637
更新日期:2003-06-30 00:00:00
abstract::A procedure for measuring the rate of heat production from a fermentation has been developed. The method is based on measuring the rate of temperature rise of the fermentation broth resulting from metabolism, when the temperature controller is turned off. The heat accumulation measured in this manner is then corrected...
journal_title:Biotechnology and bioengineering
pub_type: 传,古典文章,历史文章,杂志文章
doi:10.1002/(sici)1097-0290(20000320)67:6<691::aid-bit
更新日期:2000-03-20 00:00:00
abstract::One unique to detect cytotoxicity is to utilize reporter gene assays for promoters that respond to stress-induced effects. In the present study, we discovered that the DNA sequence from nt -287 to +110 of the heat shock protein 70B' (HSP70B') gene could be used as a functional promoter to detect cytotoxicity of cadmiu...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20601
更新日期:2005-11-20 00:00:00
abstract::Enzymatic hydrolysis of arabinoxylan is an important prerequisite for the utilization of hemicellulose for ethanol fermentation or for making the low calorie sweetener xylitol by catalytic hydrogenation of the generated xylose. This study focus on cloning and characterization of two industrial relevant beta-xylosidase...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20908
更新日期:2006-08-05 00:00:00
abstract::A set of eight closely related plasmid constructs carrying CI857-controlled recombinant genes has been used as a model to study plasmid stability in Escherichia coli, in the absence of antibiotic selection. Plasmid loss rates and relative interdivision times of plasmid-bearing cells and plasmid-free cells have been an...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:1998-06-20 00:00:00
abstract::Fluorotelomer alcohols [FTOHs, F(CF(2))(n) CH(2)CH(2)OH, n = 4, 6, and 8] are emerging environmental contaminants. Biotransformation of FTOHs by mixed bacterial cultures has been reported; however, little is known about the microorganisms responsible for the biotransformation. Here we reported biotransformation of FTO...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24561
更新日期:2012-12-01 00:00:00
abstract::It is shown that the mass transfer resistance can significantly distort the linearity of the Lineweaver-Burk plot of the kinetic data for a microbial culture which forms aggregates. For small flocs, the linearity of the Lineweaver-Burk plot is largely retained, but a different slope and intercept will be obtained comp...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260191204
更新日期:1977-12-01 00:00:00
abstract::Surface modified bioactive glass with surface properties akin to those of the bone mineral phase is an attractive candidate for use as a microcarrier material for 3-D growth of bone-like tissue in rotating wall vessel bioreactors (RWVs). The critical surface properties of this material are the result of reaction in so...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10023
更新日期:2001-11-05 00:00:00