Biodefluorination and biotransformation of fluorotelomer alcohols by two alkane-degrading Pseudomonas strains.

abstract：:Chinese hamster ovary (CHO) cells are the predominant host cell line for the production of biopharmaceuticals, a growing industry currently worth more than $188 billion USD in global sales. CHO cells undergo programmed cell death (apoptosis) following different stresses encountered in cell culture, such as substrate l...

journal_title：Biotechnology and bioengineering

authors： Henry MN,MacDonald MA,Orellana CA,Gray PP,Gillard M,Baker K,Nielsen LK,Marcellin E,Mahler S,Martínez VS

更新日期：2020-04-01 00:00:00

abstract：:Dilute aqueous solutions of polymers released by marine phytoplankton (microalgae) were shown to effectively reduce drag in capillary pipe flow. Tests were performed in a capillary turbulent flow viscometer which extruded small samples under high pressures. In all, 22 species were screened, and the products of one chl...

journal_title：Biotechnology and bioengineering

authors： Ramus J,Kenney BE,Shaughnessy EJ

更新日期：1989-01-25 00:00:00

abstract：:We report the assembly of seven different antibodies (and two antigens) into functional supramolecular structures that are specifically designed to facilitate integration into devices using entirely biologically based bottom-up fabrication. This is enabled by the creation of an engineered IgG-binding domain (HG3T) wit...

journal_title：Biotechnology and bioengineering

authors： Wu HC,Shi XW,Tsao CY,Lewandowski AT,Fernandes R,Hung CW,DeShong P,Kobatake E,Valdes JJ,Payne GF,Bentley WE

更新日期：2009-06-01 00:00:00

abstract：:Rapid advances in intensifying upstream processes for biologics production have left downstream processing as a bottleneck in the manufacturing scheme. Biomanufacturers are pursuing continuous downstream process development to increase efficiency and flexibility, reduce footprint and cost of goods, and improve product...

journal_title：Biotechnology and bioengineering

authors： Somasundaram B,Pleitt K,Shave E,Baker K,Lua LHL

更新日期：2018-12-01 00:00:00

abstract：:Alkyl beta-D-glucosides were synthesized from D-glucose and alcohols by reverse hydrolysis using the commercially available almond beta-D-glucosidase in 9:1 (v/v) acetonitrile-water medium. The main characteristics of this enzyme-catalyzed glucosylation were established by using 2-hydroxybenzyl alcohol. The reaction i...

journal_title：Biotechnology and bioengineering

authors： Vic G,Biton J,Le Beller D,Michel JM,Thomas D

更新日期：1995-04-20 00:00:00

abstract：:After periodate oxidation of its glycosidic component, invertase was covalently bound onto three types of modified solid supports: glycidyl methacrylate, styrene-divinylbenzene copolymers, and bead cellulose. Direct reaction of the invertase aldehyde groups that were formed with amino groups of the support and use of ...

journal_title：Biotechnology and bioengineering

authors： Marek M,Valentová O,Kás J

更新日期：1984-10-01 00:00:00

abstract：:Production of cellulase using solid culture systems of Trichoderma reesei QM9414 and Sporotrichum cellulophilum on wheat bran was studied. By using moisture-controlled solid culture equipment, the effect of water content of wheat bran on cell growth and cellulase production was investigated. Cellular biomass grown on ...

journal_title：Biotechnology and bioengineering

authors： Kim JH,Hosobuchi M,Kishimoto M,Seki T,Yoshida T,Taguchi H,Ryu DD

更新日期：1985-10-01 00:00:00

abstract：:Glycerol is considered as an ideal feedstock for producing bioplastics via bacterial fermentation due to its ubiquity, low price, and high degree of reduction substrate. In this work, we study the yield and cause of limitation in poly(3-hydroxybutyrate) (PHB) production from glycerol. Compared to glucose-based PHB pro...

journal_title：Biotechnology and bioengineering

authors： Tanadchangsaeng N,Yu J

更新日期：2012-11-01 00:00:00

abstract：:A new method for solid-state ethanol fermentation (the SSEF system) was experimented on for the ethanol production from solid starchy materials, where a packedbed-type fermentor was used. Both cultivation of Aspergillus saitoi and enrichment of a saccharifying enzyme were effective for hydrolysis of the starch. Ethano...

journal_title：Biotechnology and bioengineering

authors： Sato K,Nakamura K,Sato S

更新日期：1985-09-01 00:00:00

abstract：:The twin arginine translocation (Tat) pathway occurs naturally in E. coli and has the distinct ability to translocate folded proteins across the inner membrane of the cell. It has the potential to export commercially useful proteins that cannot be exported by the ubiquitous Sec pathway. To better understand the biopro...

journal_title：Biotechnology and bioengineering

authors： Branston SD,Matos CF,Freedman RB,Robinson C,Keshavarz-Moore E

更新日期：2012-04-01 00:00:00

abstract：:Somatic embryogenesis is among the most promising means of large scale plant micropropagation. The development of somatic embryos is characterized by their morphological changes. Embryos in culture usually exhibit high heterogeneity and abnormality. As conventional microscopic observation is laborious and subjective, ...

journal_title：Biotechnology and bioengineering

authors： Chi CM,Zhang C,Staba EJ,Cooke TJ,Hu WS

更新日期：1996-04-05 00:00:00

abstract：:A novel endoglucanase gene, celM , was cloned from a thermal spring metagenome. The gene was expressed in Escherichia coli, and the protein was extracted and purified. The protein catalyzed the hydrolysis of amorphous cellulose in a wide range of temperatures, 30-95°C, with optimal activity at 80°C. It was able to tol...

journal_title：Biotechnology and bioengineering

authors： Joshi N,Kaushal G,Singh SP

更新日期：2021-01-06 00:00:00

abstract：:A novel fed-batch approach for the production of L-phenylalanine (L-Phe) with recombinant E. coli is presented concerning the on-line control of the key fermentation parameters glucose and tyrosine. Two different production strains possessing either the tyrosine feedback resistant aroF(fbr) (encoding tyrosine feedback...

journal_title：Biotechnology and bioengineering

authors： Gerigk M,Bujnicki R,Ganpo-Nkwenkwa E,Bongaerts J,Sprenger G,Takors R

更新日期：2002-12-30 00:00:00

abstract：:A novel chemistry has been developed for the production of capsules composed of a hydrophobic liquid core surrounded by a cross-linked polyacrylamide/alginate membrane. These liquid-core capsules may be used in capsular perstraction for the removal of inhibitory products from bioprocesses and bioconversions. They have...

journal_title：Biotechnology and bioengineering

authors： Wyss A,von Stockar U,Marison IW

更新日期：2004-06-05 00:00:00

abstract：:Enzyme clustering into compact agglomerates could accelerate the processing of intermediates to enhance metabolic pathway flux. However, enzyme clustering is still a challenging task due to the lack of universal assembly strategy applicable to all enzymes. Therefore, we proposed an alternative enzyme assembly strategy...

journal_title：Biotechnology and bioengineering

authors： Lv X,Jin K,Wu Y,Zhang C,Cui S,Zhu X,Li J,Du G,Liu L

更新日期：2020-05-01 00:00:00

abstract：:Rhodococcus rhodochrous IGTS8 was previously isolated because of its ability to use coal as its sole source of sulfur for growth. Subsequent growth studies have revealed that IGTS8 is capable of using a variety of organosulfur compounds as sources of sulfur but not carbon. In this article, the ability of IGTS8 to sele...

journal_title：Biotechnology and bioengineering

authors： Kilbane JJ 2nd,Jackowski K

更新日期：1992-11-01 00:00:00

abstract：:For clinical trials, large amounts of high-titer retroviral supernatants are required. However, retroviral concentration is relatively low compared with other viral vectors. Moreover, less than half of retroviral vectors suspended in a collected supernatant are infectious because of their short half-lives. In this stu...

journal_title：Biotechnology and bioengineering

authors： Kwon YJ,Peng CA

更新日期：2005-05-05 00:00:00

abstract：:Effectively releasing the locked polysaccharides from recalcitrant lignocellulose to fermentable sugars is among the greatest technical and economic barriers to the realization of lignocellulose biorefineries because leading lignocellulose pre-treatment technologies suffer from low sugar yields, and/or severe reaction...

journal_title：Biotechnology and bioengineering

authors： Zhang YH,Ding SY,Mielenz JR,Cui JB,Elander RT,Laser M,Himmel ME,McMillan JR,Lynd LR

更新日期：2007-06-01 00:00:00

abstract：:In metabolic engineering, systems which allow coordinated control of two metabolic pathways can be useful. We designed two expression systems and demonstrated their application by coordinating glycogen synthesis and degradation. The first expression vector pMSW2 expressed the glycogen synthesis genes in one operon and...

journal_title：Biotechnology and bioengineering

authors： Dedhia N,Chen W,Bailey JE

更新日期：1997-07-20 00:00:00

abstract：:This study conducted quantitative kinetic modeling and in situ and temporally resolved measurements of adsorption, desorption, and re-adsorption of a commercial endoglucanase in lignocellulosic suspensions. The study defined a cellulase adsorption and desorption competition parameter, a pseudo rate of binding and deso...

journal_title：Biotechnology and bioengineering

authors： Wang QQ,Zhu JY,Hunt CG,Zhan HY

更新日期：2012-08-01 00:00:00

abstract：:Macroencapsulation technology has been an attractive topic in the field of treatment for Type 1 diabetes due to mechanical stability, versatility, and retrievability of the macro-capsule design. Macro-capsules can be categorized into extravascular and intravascular devices, in which solute transport relies either on d...

journal_title：Biotechnology and bioengineering

authors： Song S,Roy S

更新日期：2016-07-01 00:00:00

abstract：:Biofilms commonly develop in flowing aqueous environments, where the flow causes the biofilm to deform. Because biofilm deformation affects the flow regime, and because biofilms behave as complex heterogeneous viscoelastic materials, few models are able to predict biofilm deformation. In this study, a phase-field (PF)...

journal_title：Biotechnology and bioengineering

authors： Li M,Matouš K,Nerenberg R

更新日期：2020-07-13 00:00:00

abstract：:Cost-effective production of biopharmaceuticals on a large scale can be carried out by perfusion cultures of mammalian cells. One problem with this mode of operation for submerged free-cell cultures is the requirement for an efficient cell separation device located in the effluent stream. The present work investigates...

journal_title：Biotechnology and bioengineering

authors： Docoslis A,Kalogerakis N,Behie LA,Kaler KV

更新日期：1997-05-05 00:00:00

abstract：:An alkali stable polyamidase was isolated from a new strain of Nocardia farcinica. The enzyme consists of four subunits with a total molecular weight of 190 kDa. The polyamidase cleaved amide and ester bonds of water insoluble model substrates like adipic acid bishexylamide and bis(benzoyloxyethyl)terephthalate and hy...

journal_title：Biotechnology and bioengineering

authors： Heumann S,Eberl A,Fischer-Colbrie G,Pobeheim H,Kaufmann F,Ribitsch D,Cavaco-Paulo A,Guebitz GM

更新日期：2009-03-01 00:00:00

abstract：:Pancreatic islet transplantation is a promising alternative to whole-pancreas transplantation as a treatment of type 1 diabetes mellitus. This technique has been extensively developed during the past few years, with the main purpose of minimizing the complications arising from the standard protocols used in organ tran...

journal_title：Biotechnology and bioengineering

authors： Perez-Basterrechea M,Esteban MM,Vega JA,Obaya AJ

更新日期：2018-12-01 00:00:00

abstract：:Diffusion of phenol through a biofilm attached to activated carbon particles was investigated. The biofilm was grown on activated carbon particles in a draft-tube three-phase fluidized-bed bioreactor operating in a fed-batch mode. It was found that phenol did not adsorb on the biofilm and that the diffusion coefficien...

journal_title：Biotechnology and bioengineering

authors： Fan LS,Leyva-Ramos R,Wisecarver KD,Zehner BJ

更新日期：1990-02-05 00:00:00

abstract：:Gene deletion strategies using flux balance analysis (FBA) have improved the growth-coupled production of various compounds. However, the productivities were often below the expectation because the cells failed to adapt to these genetic perturbations. Here, we demonstrate the productivity of the succinate of the desig...

journal_title：Biotechnology and bioengineering

authors： Tokuyama K,Toya Y,Horinouchi T,Furusawa C,Matsuda F,Shimizu H

更新日期：2018-06-01 00:00:00

abstract：:A new high throughput technique for enzyme characterization with specific attention to the long term stability, called "Enzyme Test Bench," is presented. The concept of the Enzyme Test Bench consists of short term enzyme tests in 96-well microtiter plates under partly extreme conditions to predict the enzyme long term...

journal_title：Biotechnology and bioengineering

authors： Rachinskiy K,Schultze H,Boy M,Bornscheuer U,Büchs J

更新日期：2009-06-01 00:00:00

abstract：:In the present investigation molecular sieve has been found to be a suitable carrier for the immobilization of enzymes. From the present study it may be specifically found that trypsin and pappain can be immobilized by molecular sieve type 4a following a very simple techniqure. The immobilized enzyme can be used both ...

journal_title：Biotechnology and bioengineering

authors： Mukherjea RN,Bhattacharya P,Ghosh BK,Taraphdar DK

更新日期：1977-09-01 00:00:00

abstract：:The methanotrophic bacterium Methylococcus capsulatus is capable of assimilating methane and oxygen into protein-rich biomass, however, the diverse metabolism of the microorganism also allows for several undesired cometabolic side-reactions to occur. In this study, the ammonia cometabolism in Methylococcus capsulatus ...

journal_title：Biotechnology and bioengineering

authors： Petersen LAH,Lieven C,Nandy SK,Villadsen J,Jørgensen SB,Christensen I,Gernaey KV

更新日期：2019-11-01 00:00:00