Fractionating recalcitrant lignocellulose at modest reaction conditions.

abstract：:The microscopic morphology of Trichoderma reesei QM 9414, growing in submerged culture, was studied by image analysis. The morphology was characterized by the total hyphal length, the total number of tips, the number of actively growing tips, and the length of the main hypha. To describe the growth of a single myceliu...

journal_title：Biotechnology and bioengineering

authors： Lejeune R,Nielsen J,Baron GV

更新日期：1995-09-05 00:00:00

abstract：:We study the role of nano-scale cues in controlling neuronal growth. We use photolithography to fabricate substrates with repeatable line-pattern ridges of nano-scale heights. We find that neuronal processes, which are of micron size, have strong interactions with ridges even as low as 10 nm. The interaction between t...

journal_title：Biotechnology and bioengineering

authors： Baranes K,Chejanovsky N,Alon N,Sharoni A,Shefi O

更新日期：2012-07-01 00:00:00

abstract：:An efficient method was developed for media separation and cell collection for eukaryotic cells growing in suspension. The method is based on tangential flow microfiltration using an open channel arrangement in a hollow fiber configuration. Best results (highest processing flux rate) for polysulfone hollow fibers were...

journal_title：Biotechnology and bioengineering

authors： Trinh L,Shiloach J

更新日期：1995-11-20 00:00:00

abstract：:The emissions of nitrous oxide (N(2)O) and nitric oxide (NO) from biological nitrogen removal (BNR) operations via nitrification and denitrification is gaining increased prominence. While many factors relevant to the operation of denitrifying reactors can influence N(2)O and NO emissions from them, the role of differe...

journal_title：Biotechnology and bioengineering

authors： Lu H,Chandran K

更新日期：2010-06-15 00:00:00

abstract：:Mechanical forces regulate the structure and function of many tissues in vivo; recent results indicate that the mechanical environment can decisively influence the development of engineered tissues cultured in vitro. To investigate the effects of the hydrodynamic environment on tissue-engineered cartilage, primary bov...

journal_title：Biotechnology and bioengineering

authors： Gooch KJ,Kwon JH,Blunk T,Langer R,Freed LE,Vunjak-Novakovic G

更新日期：2001-02-20 00:00:00

abstract：:Clostridium formicoaceticum homofermentatively converts lactate to acetate at 37 degrees C and pH 6.6-9.6. However, this fermentation is strongly inhibited by acetic acid at acidic pH. The specific growth rate of this organism decreased from a maximum at pH 7.6 to zero at pH 6.6. This inhibition effect was found to be...

journal_title：Biotechnology and bioengineering

authors： Tang IC,Okos MR,Yang ST

更新日期：1989-10-20 00:00:00

abstract：:Lipase (EC 3.1.1.3., from Pseudomonas sp.) was entrapped in collagen membrane containing liquid crystal (4-methoxybenzilidene-4'-n-butylaniline). The activity of the lipase-liquid crystal membrane at an applied voltage of 4 V was 3.4 compared to a membrane tested without imposition of an external electric field. A lin...

journal_title：Biotechnology and bioengineering

authors： Karube I,Yugeta Y,Suzuki S

更新日期：1977-10-01 00:00:00

abstract：:Many biochemical processes consist of a sequence of operations for which optimal operating conditions (setpoints) have to be determined. If such optimization is performed for each operation separately with respect to objectives defined for each operation individually, overall process performance is likely to be subopt...

journal_title：Biotechnology and bioengineering

authors： Groep ME,Gregory ME,Kershenbaum LS,Bogle ID

更新日期：2000-02-05 00:00:00

abstract：:Microfluidic bioreactors have been shown valuable for various cellular applications. The use of micro-wells/grooves bioreactors, in which micro-topographical features are used to protect sensitive cells from the detrimental effects of fluidic shear stress, is a promising approach to culture sensitive cells in these pe...

journal_title：Biotechnology and bioengineering

authors： Korin N,Bransky A,Khoury M,Dinnar U,Levenberg S

更新日期：2009-03-01 00:00:00

abstract：:Batch fermentations of glucose to ethanol by Z. Mobilis.(ATCC 10988) were examined in several semidefined nutrient media. The measurement of acid produced by the microorganism was used to study its transient fermentation characteristics. Limitation of nitrogen source in the semidefined medium of Rogers and coworkers(2...

journal_title：Biotechnology and bioengineering

authors： Veeramallu U,Agrawal P

更新日期：1988-05-20 00:00:00

abstract：:The thermo-responsive polymer poly(N-isopropylacrylamide) has received widespread attention for its in vitro application in the non-invasive, non-destructive release of adherent cells on two dimensional surfaces. In this study, 3D non-woven scaffolds fabricated from poly(propylene) (PP), poly(ethylene terephthalate) (...

journal_title：Biotechnology and bioengineering

authors： Rossouw CL,Chetty A,Moolman FS,Birkholtz LM,Hoppe H,Mancama DT

更新日期：2012-08-01 00:00:00

abstract：:Feasibility of elemental sulfur reduction by Desulfovibrio desulfuricans in anaerobic conditions in a stirred reactor was studied. Hydrogen was used as energy source, whereas the carbonated species were bicarbonate and yeast extract. Attention was paid to reactor engineering aspects, biofilm formation on the sulfur su...

journal_title：Biotechnology and bioengineering

authors： Escobar C,Bravo L,Hernández J,Herrera L

更新日期：2007-10-15 00:00:00

abstract：:Increasing the throughput and efficiency of cell culture process development has become increasingly important to rapidly screen and optimize cell culture media and process parameters. This study describes the application of a miniaturized bioreactor system as a scaled-down model for cell culture process development u...

journal_title：Biotechnology and bioengineering

authors： Chen A,Chitta R,Chang D,Amanullah A

更新日期：2009-01-01 00:00:00

abstract：:Measurements of k(L)a were carried out in 1. 5- and 5-L New Brunswick Scientific CelliGen bioreactors. The measured k(L)a in water were identical for both vessel sizes operated in similar condition. The mass transfer rate increased with temperature, mixing speed, and aeration rate, with this last parameter being the m...

journal_title：Biotechnology and bioengineering

authors： Johnson M,André G,Chavarie C,Archambault J

更新日期：1990-01-05 00:00:00

abstract：:This article describes a methodology that implements a Markov decision process (MDP) optimization technique in a real time fed-batch experiment. Biological systems can be better modeled under the stochastic framework and MDP is shown to be a suitable technique for their optimization. A nonlinear input/output model is ...

journal_title：Biotechnology and bioengineering

authors： Saucedo VM,Karim MN

更新日期：1997-07-20 00:00:00

abstract：:Pretreatment of sugar cane bagasse is essential for a simultaneous saccharification and fermentation (SSF) process which uses recombinant Klebsiella oxytoca strain P2 and Genencor Spezyme CE. Strain P2 has been genetically engineered to express Zymomonas mobilis genes encoding the ethanol pathway and retains the nativ...

journal_title：Biotechnology and bioengineering

authors： Doran JB,Aldrich HC,Ingram LO

更新日期：1994-06-20 00:00:00

abstract：:Differences in plasmid retention and expression are studied in both suspended and biofilm cultures of Escherichia coli DH5alpha(PMJR1750). An alternative mathematical model is proposed which allows the determination of plasmid loss probability in both suspended batch and continuously fed biofilm cultures. In our exper...

journal_title：Biotechnology and bioengineering

authors： Huang CT,Peretti SW,Bryers JD

更新日期：1993-01-20 00:00:00

abstract：:High-level expression of recombinant penicillin acylase (PAC) using the strong trc promoter system in Escherichia coli is frequently limited by the processing and folding of PAC precursors (proPAC) in the periplasm, resulting in physiological stress and inclusion body formation in this compartment. Periplasmic heat-sh...

journal_title：Biotechnology and bioengineering

authors： Wu MS,Pan KL,Chou CP

更新日期：2007-04-01 00:00:00

abstract：:Alginate microparticles produced by emulsification/internal gelation were investigated as a promising carrier for insulin delivery. The procedure involves the dispersion of alginate solution containing insulin protein, into a water immiscible phase. Gelation is triggered in situ by instantaneous release of ionic calci...

journal_title：Biotechnology and bioengineering

authors： Reis CP,Ribeiro AJ,Neufeld RJ,Veiga F

更新日期：2007-04-01 00:00:00

abstract：:Reducing viscosities of concentrated solutions of therapeutic proteins is important for their subcutaneous and intravenous delivery. Although inorganic salts and optimizing the pH were previously reported to dramatically lower the viscosity of a monoclonal antibody solution, herein we have determined these effects not...

journal_title：Biotechnology and bioengineering

authors： Du W,Klibanov AM

更新日期：2011-03-01 00:00:00

abstract：:Short-chain carboxylic acids generated by various mixed- or pure-culture fermentation processes have been considered valuable precursors for production of bioalcohols. While conversion of carboxylic acids into alcohols is routinely performed with catalytic hydrogenation or with strong chemical reducing agents, here, a...

journal_title：Biotechnology and bioengineering

authors： Perez JM,Richter H,Loftus SE,Angenent LT

更新日期：2013-04-01 00:00:00

abstract：:In an attempt to extend and intensify the productive periods of bioprocesses, a self-cleaning tangential filtration device was examined. Built into a special-design bioreactor, its cell retention was evaluated for continuous-flow operation with selected examples of bacteria (Escherichia coli), yeasts (Sacharomyces cer...

journal_title：Biotechnology and bioengineering

authors： Naja G,Volesky B,Schnell A

更新日期：2006-11-05 00:00:00

abstract：:An algorithm for operating a fed-batch fermentor at an optimum specific fermentation rate is proposed. It does not require on-line measurement of nutrient concentration in the culture medium. An on-line estimate of the specific fermentation rate is sufficient for implementation of this scheme. The algorithm is model i...

journal_title：Biotechnology and bioengineering

authors： Agrawal P,Koshy G,Ramseier M

更新日期：1989-01-05 00:00:00

abstract：:Polyhydroxyalkanoates (PHA) terployesters P(3HB-co-3HV-co-3HHx) consisting of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxyhexanoate (3HHx) were produced by wild-type Aeromonas hydrophila 4AK4, its recombinant harboring PHA synthesis genes phaPCJ encoding PHA binding protein phasin, PHA synthase, and...

journal_title：Biotechnology and bioengineering

authors： Zhang HF,Ma L,Wang ZH,Chen GQ

更新日期：2009-10-15 00:00:00

abstract：:Nucleotide sugar-dependent ("Leloir") glycosyltransferases (GTs), represent a new paradigm for the application of biocatalytic glycosylations to the production of fine chemicals. However, it remains to be shown that GT processes meet the high efficiency targets of industrial biotransformations. We demonstrate in this ...

journal_title：Biotechnology and bioengineering

authors： Schmölzer K,Lemmerer M,Gutmann A,Nidetzky B

更新日期：2017-04-01 00:00:00

abstract：:By investigating the effects of four operating variables-volume (V), Ultrafiltration flux (J), enzyme concentration (E), and substrate concentration (S)-on capacity (K) and conversion rate (epsilon) of a hollow fiber CSTR, the performances of the CSTR and the kinetic constants of the reaction were determined. A model ...

journal_title：Biotechnology and bioengineering

authors： Bressollier P,Petit JM,Julien R

更新日期：1988-05-01 00:00:00

abstract：:Aggregates of Escherichia coli confined within hollowfiber reactors were either formed in place by culturing cells within the reactors, or were prepared by injecting a cell suspension that had been concentrated by centrifugation. The effective diffusive permeability of an uncharged nonreacting tracer, nitrous oxide, w...

journal_title：Biotechnology and bioengineering

authors： Libicki SB,Salmon PM,Robertson CR

更新日期：1988-06-20 00:00:00

abstract：:A biodevice involving thiolated ssDNA and engineered cytochrome b5 linked through a cis-platine bridge is described. This original nanostructure is associated to a supported membrane through a floating anchor, thus constituting a dynamic bidimensionnal DNA capture device contrasting with the constrained geometry of cu...

journal_title：Biotechnology and bioengineering

authors： Boireau W,Bombard S,Sari MA,Pompon D

更新日期：2002-01-20 00:00:00

abstract：:This communication demonstrates that two-phase aqueous mixed (nonionic/ionic) micellar systems have the potential for improving the separation of proteins from viruses. Specifically, two separation experiments were performed to show that the addition of the anionic surfactant sodium dodecyl sulfate (SDS) to the two-ph...

journal_title：Biotechnology and bioengineering

authors： Kamei DT,King JA,Wang DI,Blankschtein D

更新日期：2002-10-20 00:00:00

abstract：:Cancer is the leading cause of death in industrialized countries. Cancer therapy often involves monoclonal antibodies or small-molecule drugs, but carbohydrate-binding lectins such as mistletoe (Viscum album) viscumin offer a potential alternative treatment strategy. Viscumin is toxic in mammalian cells, ruling them o...

journal_title：Biotechnology and bioengineering

authors： Gengenbach BB,Keil LL,Opdensteinen P,Müschen CR,Melmer G,Lentzen H,Bührmann J,Buyel JF

更新日期：2019-09-01 00:00:00