Design of expression systems for metabolic engineering: coordinated synthesis and degradation of glycogen.


:In metabolic engineering, systems which allow coordinated control of two metabolic pathways can be useful. We designed two expression systems and demonstrated their application by coordinating glycogen synthesis and degradation. The first expression vector pMSW2 expressed the glycogen synthesis genes in one operon and the glycogen degradation gene in a separate, coordinately regulated operon. The plasmid was designed to switch off expression of the first operon and activate expression of the second operon on addition of IPTG. As an alternative means to control glycogen synthesis and degradation pathways, we constructed expression vector pGTSD100, which contains the native Escherichia coli glycogen synthesis and degradation operon under control of the tac promoter. Both expression vectors work successfully to control the net synthesis and degradation of glycogen. In cultures of the E. coli strain TA3476 carrying the plasmid pMSW2, before the addition of IPTG, glycogen continued to accumulate in the culture. About three hours after IPTG was added, glycogen levels began to decrease. When no IPTG was added to cultures of TA3476:pMSW2, glycogen accumulated in the cells as before but the rate of degradation of glycogen was much lower. When IPTG was added to TA3476:pMSW2, the total cell protein at the end of batch cultivation was approximately 15% higher compared to cultures without IPTG addition. The extra biomass was formed during the glycogen degradation phase. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55: 419-426, 1997.


Biotechnol Bioeng


Dedhia N,Chen W,Bailey JE




Has Abstract


1997-07-20 00:00:00












  • Medium-induced inhibition of microbial adsorption to nickel and activated charcoal.

    abstract::Equilibrium adsorption studies on Escherichia coli and Saccharomyces sp. revealed the capacity and affinity of these organisms for the surfaces of powdered charcoal and nickel. In simple salt solutions both organisms readily adsorbed to each solid with an affinity and maximum loading capacity individual to each cell-s...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Protheroe RG,Cumming RH,Matchett A

    更新日期:1989-10-05 00:00:00

  • Fractional factorial design to investigate stromal cell regulation of macrophage plasticity.

    abstract::Understanding the regulatory networks which control specific macrophage phenotypes is essential in identifying novel targets to correct macrophage mediated clinical disorders, often accompanied by inflammatory events. Since mesenchymal stromal cells (MSCs) have been shown to play key roles in regulating immune functio...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Barminko JA,Nativ NI,Schloss R,Yarmush ML

    更新日期:2014-11-01 00:00:00

  • Permeability of alginate microcapsules to secretory recombinant gene products.

    abstract::Non-autologous somatic gene therapy is an alternate approach to delivering recombinant gene products through implantation of a "universal" donor cell line engineered to produce a therapeutic gene product. The cells are immunologically isolated by enclosure in immunoprotective microcapsules fabricated from alginate-pol...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Awrey DE,Tse M,Hortelano G,Chang PL

    更新日期:1996-11-20 00:00:00

  • Measurement of heat evolution and correlation with oxygen consumption during microbial growth. Reprinted from Biotechnology and Bioengineering, Vol. XI, Issue 3, Pages 269-281 (1968).

    abstract::A procedure for measuring the rate of heat production from a fermentation has been developed. The method is based on measuring the rate of temperature rise of the fermentation broth resulting from metabolism, when the temperature controller is turned off. The heat accumulation measured in this manner is then corrected...

    journal_title:Biotechnology and bioengineering

    pub_type: 传,古典文章,历史文章,杂志文章


    authors: Cooney CL,Wang DI,Mateles RI

    更新日期:2000-03-20 00:00:00

  • Rapid renaturation of denatured and aggregated proteins using liquid paraffin as a pseudolipid bilayer membrane.

    abstract::A means of rapidly renaturing denatured protein was devised and evaluated. Three liquids were laminarly layered in a centrifuge tube, in which two solutions sandwiched liquid paraffin so as to form a pseudolipid bilayer. Denatured and aggregted protein placed on the upper surface of liquid paraffin was renatured as it...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Yoshii H,Kojima T,Furuta T,Yasunishi A

    更新日期:1994-01-05 00:00:00

  • Characteristics and role of dynamic membrane layer in anaerobic membrane bioreactors.

    abstract::A submerged anaerobic dynamic membrane bioreactor (AnDMBR) was operated for treatment of concentrated wastewater. The dynamic membrane (DM) or cake layer was characterized on its physicochemical and biological composition and the role of the DM layer in treatment and filtration performances was assessed. The results s...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ersahin ME,Tao Y,Ozgun H,Spanjers H,van Lier JB

    更新日期:2016-04-01 00:00:00

  • Main and interaction effects of acetic acid, furfural, and p-hydroxybenzoic acid on growth and ethanol productivity of yeasts.

    abstract::The influence of the factors acetic acid, furfural, and p-hydroxybenzoic acid on the ethanol yield (YEtOH) of Saccharomyces cerevisiae, bakers' yeast, S. cerevisiae ATCC 96581, and Candida shehatae NJ 23 was investigated using a 2(3)-full factorial design with 3 centrepoints. The results indicated that acetic acid inh...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Palmqvist E,Grage H,Meinander NQ,Hahn-Hägerdal B

    更新日期:1999-04-05 00:00:00

  • Scaleup of spinfilter perfusion bioreactor for mammalian cell retention.

    abstract::A spinning cylindrical filter, known as a spinfilter, permits the mammalian cell bioreactor operation at high perfusion rates leading to very high cell densities (10(7) mL(-1)). Filter screens with openings (25 mum) slightly larger than the average cell size have been used to retain single cells in suspension over a l...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Yabannavar VM,Singh V,Connelly NV

    更新日期:1994-01-20 00:00:00

  • INT-dehydrogenase test for activated sludge process control.

    abstract::Dehydrogenase activity assay of activated sludge using the redox dye 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride (INT) was investigated. INT-dehydrogenase activity (INT-DHA) was directly proportional to INT dosage and inversely proportional to bio-mass concentration over limited ranges. INT dosages...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Lopez JM,Koopman B,Bitton G

    更新日期:1986-07-01 00:00:00

  • Optimal production of glutathione by controlling the specific growth rate of yeast in fed-batch culture.

    abstract::The optimal of the specific growth rate was obtained with simple mathematical model in a yeast fed-batch cultures. The model was based on the mass balance around the fed-batch system and the relationship between the specific growth rate, mu, and the specific production rate of glutathione, rho(G). The optimal profile ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Shimizu H,Araki K,Shioya S,Suga K

    更新日期:1991-06-20 00:00:00

  • Bromoperoxidase activity in microplantlet suspension cultures of the macrophytic red alga Ochtodes secundiramea.

    abstract::Bromoperoxidase is an enzyme found in marine macroalgae that catalyzes the bromination of organic substrates. Photosynthetic microplantlet suspension cultures derived from the macrophytic red alga Ochtodes secundiramea were shown to possess bromoperoxidase. The optimum pH for O. secundiramea bromoperoxidase activity i...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Rorrer GL,Tucker MP,Cheney DP,Maliakal S

    更新日期:2001-09-05 00:00:00

  • Metabolic network analysis of Penicillium chrysogenum using (13)C-labeled glucose.

    abstract::Using (13)C-labeled glucose fed to a penicillin-overproducing strain of Penicillium chrysogenum, the intracellular fluxes were quantified, and the presence of two new pathways, not previously described in this organism, is suggested. Thus, glycine was synthesized not only by serine hydroxymethyltransferase, but also b...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Christensen B,Nielsen J

    更新日期:2000-06-20 00:00:00

  • Nisin-inducible secretion of a biologically active single-chain insulin analog by Lactococcus lactis NZ9000.

    abstract::Oral delivery of insulin to diabetic patients is highly desirable because it would be non-invasive and more closely mimic normal physiology, but this route of administration typically results in low bioavailability due to low pH and enzymatic degradation along the gastrointestinal tract. To explore an alternative appr...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ng DT,Sarkar CA

    更新日期:2011-08-01 00:00:00

  • Rapid in vivo evolution of a beta-lactamase using phagemids.

    abstract::RNA viruses are capable of undergoing extremely rapid evolution due to their high rates of reproduction, small genome size, and a high frequency of spontaneous mutagenesis. Here we demonstrate that a virus-like, evolutionary state can be created by propagating a phagemid population in a hypermutator strain of Escheric...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Long-McGie J,Liu AD,Schellenberger V

    更新日期:2000-04-05 00:00:00

  • Expression of recombinant protein A from the lac promoter in Escherichia coli JM83 is not subject to catabolite repression when grown under specific conditions of continuous culture.

    abstract::Although widely used in experimental and industrial situations, genetically engineered plasmids containing the lac promoter from Escherichia coli are subject to catabolite repression when grown in glucose-containing media. Several methods of overcoming this problem have been investigated by studying the expression of ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Warnes A,Stephenson JR,Fooks AR,Melling J,Brown MR

    更新日期:1991-11-01 00:00:00

  • Comparison of glucose fermentation by suspended and gel-entrapped yeast cells: An in vivo nuclear magnetic resonance study.

    abstract::Phosphorus-31 nuclear magnetic resonance ((31)P NMR) was used to compare the anaerobic metabolism of glucose by suspended and gel-entrapped Saccharomyces bayanus cells. The fermentation of glucose was carried out in a reaction system with continuous circulation through the NMR sample tube. The intracellular pH and the...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Taipa MA,Cabral JM,Santos H

    更新日期:1993-03-15 00:00:00

  • Simple diffusion is the primary mechanism for glucose uptake during the production phase of the Aspergillus niger citric acid process.

    abstract::Models for the known glucose transporters in Aspergillus niger and for simple diffusion of glucose through the hyphal membrane were prepared. The results from the models were compared with fermentation data from published studies on citric acid. It was found that the purely physical and uncontrolled process of diffusi...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Wayman FM,Mattey M

    更新日期:2000-02-20 00:00:00

  • Comparative metabolite analysis to understand lactate metabolism shift in Chinese hamster ovary cell culture process.

    abstract::A metabolic shift from lactate production (LP) to net lactate consumption (LC) phenotype was observed in certain Chinese hamster ovary (CHO) cell lines during the implementation of a new chemically defined medium (CDM) formulation for antibody production. In addition, this metabolic shift typically leads to process pe...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Luo J,Vijayasankaran N,Autsen J,Santuray R,Hudson T,Amanullah A,Li F

    更新日期:2012-01-01 00:00:00

  • Influence of mass transfer limitations on determination of the half saturation constant for hydrogen uptake in a mixed-culture CH(4)-producing enrichment.

    abstract::There is strong evidence in the literature supporting the existence of significant mass transfer limitations on the kinetics of exogenous H(2) consumption by methanogens. The half saturation constant for H (2) uptake by a mixed-culture, CH(4) producing enrichment was measured using an experimental protocol that avoide...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Giraldo-Gomez E,Goodwin S,Switzenbaum MS

    更新日期:1992-10-05 00:00:00

  • The effects of methanol on the biofiltration of dimethyl sulfide in inorganic biofilters.

    abstract::Air emissions from the pulp and paper industry frequently contain reduced sulfur compounds (RSC), such as dimethyl sulfide (DMS) mixed with volatile organic compounds (VOC) (e.g., methanol, MeOH) and it is desirable to treat either one or both of these groups of compounds. The objective of this study was to assess the...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Zhang Y,Liss SN,Allen DG

    更新日期:2006-11-05 00:00:00

  • Human mesenchymal stem cell position within scaffolds influences cell fate during dynamic culture.

    abstract::Cell-based tissue engineering is limited by the size of cell-containing constructs that can be successfully cultured in vitro. This limit is largely a result of the slow diffusion of molecules such as oxygen into the interior of three-dimensional scaffolds in static culture. Bioreactor culture has been shown to overco...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Yeatts AB,Geibel EM,Fears FF,Fisher JP

    更新日期:2012-09-01 00:00:00

  • Characterization of free and alginate-polylysine-alginate microencapsulated Erwinia herbicola for the conversion of ammonia, pyruvate, and phenol into L-tyrosine.

    abstract::The whole cell tyrosine phenol-lyase (TPL, E.C. activity of Erwinia herbicola (ATCC 21434) was microen-capsulated. We studied the use of this for the conversion of ammonia and pyruvate along with phenol or catechol, respectively, into L-tyrosine or dihydroxyphenyl-L-alanine (L-dopa). The reactions are releva...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Lloyd-George I,Chang TM

    更新日期:1995-12-20 00:00:00

  • Manipulation of microparticles for construction of array patterns by negative dielectrophoresis using multilayered array and grid electrodes.

    abstract::In this study, a useful method was developed to fabricate array patterns of microparticles not on electrode surfaces, but on arbitrary surfaces, using negative-dielectrophoresis (n-DEP). First, electrodes were designed and electric field simulations were performed to manipulate microparticles toward target areas. Base...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ino K,Shiku H,Ozawa F,Yasukawa T,Matsue T

    更新日期:2009-11-01 00:00:00

  • 3D tumor spheroids as in vitro models to mimic in vivo human solid tumors resistance to therapeutic drugs.

    abstract::Three-dimensional cell culture models, such as spheroids, can be used in the process of the development of new anticancer agents because they are able to closely mimic the main features of human solid tumors, namely their structural organization, cellular layered assembling, hypoxia, and nutrient gradients. These prop...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章,评审


    authors: Nunes AS,Barros AS,Costa EC,Moreira AF,Correia IJ

    更新日期:2019-01-01 00:00:00

  • A thermodynamic model to predict Phanerochaete chrysosporium INA-12 adhesion to various solid carriers in relation to lignin peroxidase production.

    abstract::A thermodynamic model was used in this study to predict the adhesion of Phanerochaete chrysosporium INA-12 as conidiospores or mycelium to various solid carriers. Theoretical predictions were closely reflected by experimental results. Amount of immobilized mycelium was higher for hydrophobic (polypropylene and polyure...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Asther M,Bellon-Fontaine MN,Capdevila C,Corrieu G

    更新日期:1990-03-05 00:00:00

  • Determination of the baculovirus transducing titer in mammalian cells.

    abstract::Baculovirus has emerged as a promising vector for in vivo or ex vivo gene therapy. To date, the infectious titer and multiplicity of infection (MOI) based on the ability of baculovirus to infect insect cells are commonly adopted to indicate the virus dosage. However, the infectious titer and MOI do not reliably repres...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Chan ZR,Lai CW,Lee HP,Chen HC,Hu YC

    更新日期:2006-02-20 00:00:00

  • Suggestions for the operation of radial flow cells in cell adhesion and biofouling studies.

    abstract::An analysis of the radial flow cell is presented to show that the assumption of creeping laminar flow should be used cautiously. Simple models which account for the influence of fluid inertial forces over most of the width of the plate are reviewed. A modified Reynolds number is introduced which may be used to test th...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Fryer PJ,Slater NK,Duddridge JE

    更新日期:1985-04-01 00:00:00

  • Laboratory-scale production of acetoin plus diacetyl by Enterobacter cloacae ATCC 27613.

    abstract::Conditions for the laboratory-scale production of acetoin plus diacetyl by Enterobacter cloacae ATCC 27613 were studied. Thirty-five g acetoin plus diacetyl/50 g sucrose were obtained when fermentation was carried out in 2.5 liter medium containing 12.5 g peptone and 12.5 g yeast extract, at pH 7.0, in a 5 liter conic...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Gupta KG,Yadav NK,Dhawan S

    更新日期:1978-12-01 00:00:00

  • Identification and control of oxidative metabolism in Ssaccharomyces cerevisiae during transient growth using calorimetric measurements.

    abstract::The objective of this study was to characterize the dynamic adaptation of the oxidative capacity of Saccharomyces cerevisiae to an increase in the glucose supply rate and its implications for the control of a continuous culture designed to produce biomass without allowing glucose to be diverted into the reductive meta...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Duboc P,Cascão-Pereira LG,von Stockar U

    更新日期:1998-03-05 00:00:00

  • Chemotaxis increases vertical migration and apparent transverse dispersion of bacteria in a bench-scale microcosm.

    abstract::The success of in situ bioremediation is often limited by the inability to bring bacteria in contact with the pollutant, which they will degrade. A bench-scale model aquifer was used to evaluate the impact of chemotaxis on the migration of bacteria toward the source of a chemical pollutant. The model was packed with s...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Strobel KL,McGowan S,Bauer RD,Griebler C,Liu J,Ford RM

    更新日期:2011-09-01 00:00:00