Abstract:
:Lectin-like function was demonstrated in this study for a novel water-soluble polymer with phenylboronic acid residues (poly (AAPBA-DMAm)), which induced appreciable proliferation of murine spleen lymphocytes with an increased expression of interleukin-2 (IL-2) receptor on their surface. Consequently, boosted proliferation of lymphocytes with cytotoxic action to YAC-1 cells was achieved by concurrent addition of IL-2 with poly(AAPBA-DMAm) in the medium, indicating this boronate-containing polymer to be worked as an effective immuno-adjuvant for the induction of lymphokine-activated killer (LAK) cells. Flow-cytofluorimetry study revealed that poly(AAPBA-DMAm) competitively inhibited the cellular binding of N-acetylneuraminic acid-specific lectin (Limax Flavus Agglutinin) in a concentration-dependent manner, suggesting that phenylboronate moiety in the polymer may recognize N-acetylneur- aminic acid (sialic acid) residues existing on the plasma-membrane surface of lymphocytes to induce their proliferation.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Uchimura E,Otsuka H,Okano T,Sakurai Y,Kataoka Ksubject
Has Abstractpub_date
2001-02-05 00:00:00pages
307-14issue
3eissn
0006-3592issn
1097-0290pii
10.1002/1097-0290(20010205)72:3<307::AID-BIT7>3.0.journal_volume
72pub_type
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