Enzymatic synthesis of esters using an immobilized lipase.

abstract：:A detailed mechanistic model of enzymatic cellulose hydrolysis has been developed. The behavior of individual cellulase enzymes and parameters describing the cellulose surface properties are included. Results obtained for individual enzymes (T. reesei endoglucanase 2 and cellobiohydrolase I) and systems with both enzy...

journal_title：Biotechnology and bioengineering

authors： Levine SE,Fox JM,Blanch HW,Clark DS

更新日期：2010-09-01 00:00:00

abstract：:Continuous virus inactivation (VI) has received little attention in the efforts to realize fully continuous biomanufacturing in the future. Implementation of continuous VI must assure a specific minimum incubation time, typically 60 min. To guarantee the minimum incubation time, we implemented a packed bed continuous ...

journal_title：Biotechnology and bioengineering

authors： Martins DL,Sencar J,Hammerschmidt N,Flicker A,Kindermann J,Kreil TR,Jungbauer A

更新日期：2020-05-01 00:00:00

abstract：:Microcrystalline cellulose (10 g/L Avicel) was hydrolysed by two major cellulases, cellobiohydrolase I (CBH I) and endoglucanase II (EG II), of Trichoderma reesei. Two types of experiments were performed, and in both cases the enzymes were added alone and together, in equimolar mixtures. In time course studies the rea...

journal_title：Biotechnology and bioengineering

authors： Medve J,Karlsson J,Lee D,Tjerneld F

更新日期：1998-09-05 00:00:00

abstract：:A method is described to estimate the microbial biomass of a sample, to enumerate the cells, and to distinguish the portion of metabolically active cells in the population by measuring the binding of phenyldicarbaundecaborane (PCB(-)) to the cells. This method can also be used for the analysis of a complex population ...

journal_title：Biotechnology and bioengineering

authors： Daugelavicius R,Bakiene E,Berzinskiene J,Bamford DH

更新日期：2000-01-01 00:00:00

abstract：:We developed a model for nutrient removal in an aerobic granular sludge system. This model can quantitatively describe the start-up of the system by coupling a model for studying the population dynamics of the granules in the reactor (reactor-scale model) and a model for studying the microbial community structure in t...

journal_title：Biotechnology and bioengineering

authors： Kagawa Y,Tahata J,Kishida N,Matsumoto S,Picioreanu C,van Loosdrecht MC,Tsuneda S

更新日期：2015-01-01 00:00:00

abstract：:Proper analysis of label distribution in metabolic pathway intermediates is critical for correct interpretation of experimental data and strategic experimental design. While, for example, 13C nuclear magnetic resonance (NMR) spectroscopy is usually limited to the measurement of degrees of 13C enrichment, more informat...

journal_title：Biotechnology and bioengineering

authors： Klapa MI,Park SM,Sinskey AJ,Stephanopoulos G

更新日期：1999-02-01 00:00:00

abstract：:Recently it has been established that low molecular weight displacers can be successfully employed for the purification of proteins in hydrophobic interaction chromatography (HIC) systems. This work investigates the utility of this technique for the purification of an industrial protein mixture. The study involved the...

journal_title：Biotechnology and bioengineering

authors： Sunasara KM,Xia F,Gronke RS,Cramer SM

更新日期：2003-05-05 00:00:00

abstract：:A submerged anaerobic dynamic membrane bioreactor (AnDMBR) was operated for treatment of concentrated wastewater. The dynamic membrane (DM) or cake layer was characterized on its physicochemical and biological composition and the role of the DM layer in treatment and filtration performances was assessed. The results s...

journal_title：Biotechnology and bioengineering

authors： Ersahin ME,Tao Y,Ozgun H,Spanjers H,van Lier JB

更新日期：2016-04-01 00:00:00

abstract：:Immobilized enzyme hollow fibers may be useful in the purification or treatment of whole blood under clinical conditions. In this study, catalytically pure heparinase was immobilized to cellulose to analyze the feasibility for the removal of heparin's anticoagulant activity from whole blood. The kinetics of catalytica...

journal_title：Biotechnology and bioengineering

authors： Comfort AR,Albert E,Langer R

更新日期：1989-12-20 00:00:00

abstract：:Adenoviruses are human pathogens increasingly used as gene therapy and vaccination vectors. However, their impact on cell metabolism is poorly characterized. We performed carbon labeling experiments with [1,2-13 C]glucose or [U-13 C]glutamine to evaluate metabolic alterations in the amniocyte-derived, E1-transformed 1...

journal_title：Biotechnology and bioengineering

authors： Carinhas N,Koshkin A,Pais DA,Alves PM,Teixeira AP

更新日期：2017-01-01 00:00:00

abstract：:Targeted gene integration into site-specific loci can be achieved in Chinese hamster ovary (CHO) cells via CRISPR/Cas9 genome editing technology and the homology-directed repair (HDR) pathway. The low efficiency of HDR often requires antibiotic selection, which limits targeted integration of multiple genes at multiple...

journal_title：Biotechnology and bioengineering

authors： Lee JS,Grav LM,Pedersen LE,Lee GM,Kildegaard HF

更新日期：2016-11-01 00:00:00

abstract：:Bioelectrochemical systems (BES) hold great promise for sustainable energy generation via a microbial catalyst from organic matter, for example, from wastewater. To improve current generation in BES, understanding the underlying microbiology of the electrode community is essential. Electron mediator producing microorg...

journal_title：Biotechnology and bioengineering

authors： Schmitz S,Rosenbaum MA

更新日期：2018-09-01 00:00:00

abstract：:Lignocellulosic biofuels and chemicals have great potential to reduce our dependence on fossil fuels and mitigate air pollution by cutting down on greenhouse gas emissions. Chemical, thermal, and enzymatic processes are used to release the sugars from the lignocellulosic biomass for conversion to biofuels. These proce...

journal_title：Biotechnology and bioengineering

authors： Kasirajan L,Maupin-Furlow JA

更新日期：2020-11-26 00:00:00

abstract：:Capitalizing on components evolved to metabolize ethanol in Aspergillus nidulans, we previously designed the first molecular gas-gene expression interface using gaseous acetaldehyde as the major inducer. This fungus-derived acetaldehyde-inducible gene regulation (AIR) system operated perfectly and enabled precise and ...

journal_title：Biotechnology and bioengineering

authors： Weber W,Spielmann M,Daoud El-Baba M,Keller B,Aubel D,Fussenegger M

更新日期：2005-06-30 00:00:00

abstract：:Development of bioengineering using such biomaterials as plant tissues or genetically produced microorganisms makes it important to accurately estimate the prevailing shear rate in bioreactors because they are generally sensitive to shear. Various authors have discussed ways to estimate the average shear rate in a bub...

journal_title：Biotechnology and bioengineering

authors： Nishikawa M

更新日期：1991-03-25 00:00:00

abstract：:Hydrocarbon oils of the alga Botryococcus braunii, extracted from a natural "bloom" of the plant, have been hydrocracked to produce a distillate comprising 67% gasoline fraction, 15% aviation turbine fuel fraction, 15% diesel fuel fraction, and 3% residual oil. The distillate was examined by a number of standard petro...

journal_title：Biotechnology and bioengineering

authors： Hillen LW,Pollard G,Wake LV,White N

更新日期：1982-01-01 00:00:00

abstract：:Relative levels of many individual proteins in Escherichia coli HB101 strains with 0, 37, 56, and 240 plasmids per chromosome were determined by computer image analysis of two-dimensional gel electrophoresis patterns. The plasmids investigated had very similar sequences except for small domains encoding the repressor ...

journal_title：Biotechnology and bioengineering

authors： Birnbaum S,Bailey JE

更新日期：1991-04-05 00:00:00

abstract：:A simple feeding strategy was developed and successfully employed for nutritional control in a 2-L fed-batch culture of hybridoma cells. A previously developed stoichiometric model for animal cell growth was used to design a supplemental medium for feeding. Undialyzed fetal bovine serum and trace metals (Fe(2+), SeO(3...

journal_title：Biotechnology and bioengineering

authors： Xie L,Wang DI

更新日期：1996-09-20 00:00:00

abstract：:Alcoholic fermentation of cheese whey permeate was investigated using a recombinant flocculating Saccharomyces cerevisiae, expressing the LAC4 (coding for beta-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus enabling for lactose metabolization. Data on yeast fermentation and gro...

journal_title：Biotechnology and bioengineering

authors： Domingues L,Lima N,Teixeira JA

更新日期：2001-03-05 00:00:00

abstract：:Trehalase is the enzyme which hydrolyzes the disaccharide trehalose into two alpha-D-glucose molecules. In this article, we present the immobilization of trehalase on aminopropyl glass particles. The enzyme was extracted from Escherichia coli Mph2, a strain harboring the pTRE11 plasmid, which contains the trehalase ge...

journal_title：Biotechnology and bioengineering

authors： Bachinski N,Martins AS,Paschoalin VM,Panek AD,Paiva CL

更新日期：1997-04-05 00:00:00

abstract：:Utilising whole cells of recombinant Escherichia coli K27 (pCYP102, pGEc47) containing active cytochrome P450BM-3 monooxygenase [E.C. 1. 14.14.1], multiple oxidations of saturated and unsaturated fatty acids were performed by the enzyme under conditions of excess oxygen. The amount of oxygen dissolved in the culture m...

journal_title：Biotechnology and bioengineering

authors： Schneider S,Wubbolts MG,Oesterhelt G,Sanglard D,Witholt B

更新日期：1999-08-05 00:00:00

abstract：:This article provides the analysis on biomass yields and energetic yields of the oleaginous yeasts. The biomass yields of the oleaginous yeasts are consistently lower than nonoleaginous microorganisms, whereas their energetic yields are higher. Data inconsistencies of literature are explained by the variation of energ...

journal_title：Biotechnology and bioengineering

authors： Pan JG,Rhee JS

更新日期：1986-01-01 00:00:00

abstract：:A novel, alternative intensified cell culture process comprised of a linked bioreactor system is presented. An N-1 perfusion bioreactor maintained cells in a highly proliferative state and provided a continuous inoculum source to a second bioreactor operating as a continuous-flow stirred-tank reactor (CSTR). An initia...

journal_title：Biotechnology and bioengineering

authors： Gagnon M,Nagre S,Wang W,Coffman J,Hiller GW

更新日期：2019-08-01 00:00:00

abstract：:The purpose of this article is to provide a few concrete examples of the potential to acceptably reduce the scope of validation and qualification testing based on scientific justification for the specific area of microbial fermentation. The key areas explored include: autoclave operational qualification (OQ) testing, ...

journal_title：Biotechnology and bioengineering

authors： Junker B

更新日期：2001-07-05 00:00:00

abstract：:The glycosylation of pharmaglycoproteins from recombinant cell lines can be affected by an uncontrolled accumulation of ammonium in the medium. Glucosamine-6-phosphate isomerase (GPI) has been proposed as the key enzyme responsible for elevating the intracellular UDP-N-acetylhexosamine pool (UDPGNAc) by accepting ammo...

journal_title：Biotechnology and bioengineering

authors： Cayli A,Hirschmann F,Wirth M,Hauser H,Wagner R

更新日期：1999-10-20 00:00:00

abstract：:This article demonstrates the feasibility of a novel process concept for the remediation of PCB contaminated soil. The proposed process consists of PCB extraction from soil using solid polymer beads, followed by biodegradation of the extracted PCBs in a solid-liquid two-phase partitioning bioreactor (TPPB), where PCBs...

journal_title：Biotechnology and bioengineering

authors： Rehmann L,Daugulis AJ

更新日期：2008-04-01 00:00:00

abstract：:A directed differentiation between the biofilm and suspension was observed in the molecular microbial ecology and gene expression of different bacteria in a biofilm nitritation-anammox process operated at varying hydraulic residence times (HRT) and nitrogen loading rates (NLR). The highest degree of enrichment observe...

journal_title：Biotechnology and bioengineering

authors： Park H,Sundar S,Ma Y,Chandran K

更新日期：2015-02-01 00:00:00

abstract：:In recent years there has been a considerable interest in developing membrane chromatography systems that function as a short, wide chromatographic column in which the adsorptive packing consists of one or more microporous membranes. This study reports the use of new adsorber membranes prepared by the incorporation of...

journal_title：Biotechnology and bioengineering

authors： Avramescu ME,Borneman Z,Wessling M

更新日期：2003-12-05 00:00:00

abstract：:Porcine pancreatic lipase and Fusarium solani cutinase were used to catalyze transesterification reactions between methyl propionate, ethyl propionate, and a series of primary alcohols at high temperatures in a continuous packed-bed gas-solid reactor, in which the solid phase is composed of the enzyme and the substrat...

journal_title：Biotechnology and bioengineering

authors： Parvaresh F,Robert H,Thomas D,Legoy MD

更新日期：1992-02-20 00:00:00

abstract：:A novel two-stage bioreactor has been designed for a combined submerged (SF) and solid substrate fermentation (SSF) of wheat straw. The straw was pretreated with steam, and cellulases from the culture fluid of Trichoderma reesei were adsorbed on it for increased bioconvertibility. SSF was conducted in the top part of ...

journal_title：Biotechnology and bioengineering

authors： Viesturs UE,Strikauska SV,Leite MP,Berzins AJ,Tengerdy RP

更新日期：1987-08-05 00:00:00