Abstract:
:Recently it has been established that low molecular weight displacers can be successfully employed for the purification of proteins in hydrophobic interaction chromatography (HIC) systems. This work investigates the utility of this technique for the purification of an industrial protein mixture. The study involved the separation of a mixture of three protein forms, that differed in the C-terminus, from their aggregate impurities while maintaining the same relative ratio of the three protein forms as in the feed. A batch high-throughput screening (HTS) technique was employed in concert with fluorescence spectroscopy for displacer screening in these HIC systems. This methodology was demonstrated to be an effective tool for identifying lead displacer candidates for a particular protein/stationary-phase system. In addition, these results indicate that surfactants can be employed at concentrations above their CMCs as effective displacers. Displacement of the recombinant proteins with PEG-3400 and the surfactant Big Chap was shown to increase the productivity as compared to the existing step-gradient elution process.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Sunasara KM,Xia F,Gronke RS,Cramer SMdoi
10.1002/bit.10582subject
Has Abstractpub_date
2003-05-05 00:00:00pages
330-9issue
3eissn
0006-3592issn
1097-0290journal_volume
82pub_type
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