Abstract:
:Non-invasive methods for online monitoring of biotechnological processes without compromising the integrity of the reactor system are very important to generate continuous data. Even though calorimetry has been used in conventional biochemical analysis for decades, it has not yet been specifically applied for online detection of product formation at technical scale. Thus, this article demonstrates a calorespirometric method for online detection of microbial lysine formation in stirred tank bioreactors. The respective heat generation of two bacterial strains, Corynebacterium glutamicum ATCC 13032 (wild-type) and C. glutamicum DM1730 (lysine producer), was compared with the O2 -consumption in order to determine whether lysine was formed. As validation of the proposed calorespirometric method, the online results agreed well with the offline measured data. This study has proven that calorespirometry is a viable non-invasive technique to detect product formation at any time point.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Regestein L,Maskow T,Tack A,Knabben I,Wunderlich M,Lerchner J,Büchs Jdoi
10.1002/bit.24815subject
Has Abstractpub_date
2013-05-01 00:00:00pages
1386-95issue
5eissn
0006-3592issn
1097-0290journal_volume
110pub_type
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