Abstract:
:Two simultaneous fermentations were performed at 26 degrees C with simultaneous inocula using Clostridium saccharoperbutylacetonicum. Fermentation 1 prevented the gas formed by the biomass from escaping the fermentor while 2 allowed the gas formed to escape. Fermentor 1 provided for the production of butanol, acetone, and ethanol, while when the H(2) formed was allowed to escape with fermentor 2, neither butanol nor acetone were produced. Ethanol was also formed in both fermentors and began along with the initial growth of biomass and continued until the fermentations were complete. Butanol and acetone production began after biomass growth had reached a maximum and began to subside. The butanol-acetone-ethanol millimolar yields and ratios were 38:1:14 respectively. The fermentor 2 results show that a yield of 2.1 L H(2), 93 or 370 mmol H(2)/mol glucose, was formed only during the growing stage of growth; neither butanol nor acetone were produced; ethanol was formed throughout the fermentation, reaching a yield of 15.2 mmolar. It appears that hydrogen gas is required for butanol production during the resting stage of growth.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Brosseau JD,Yan JY,Lo KVdoi
10.1002/bit.260280302subject
Has Abstractpub_date
1986-03-01 00:00:00pages
305-10issue
3eissn
0006-3592issn
1097-0290journal_volume
28pub_type
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