New tools for mass isotopomer data evaluation in (13)C flux analysis: mass isotope correction, data consistency checking, and precursor relationships.

abstract：:The cellulase system of Bacteroides cellulosolvens was subjected to both catabolite repression and feedback inhibition by cellobiose. Cellulose-solubilizing activity was 50% inhibited at a cellobiose concentration of 2.6 g/L and completely inhibited by 12 g/L. Glucose at 12 g/L (the highest concentration tested) had n...

journal_title：Biotechnology and bioengineering

authors： Murray WD

更新日期：1987-06-01 00:00:00

abstract：:Multiparameter flow cytometric techniques developed in our laboratories have been used for the "at-line" study of fed-batch bacterial fermentations. These fermentations were done at two scales, production (20 m(3)) and bench (5 x 10(-3) m(3)). In addition, at the bench scale, experiments were undertaken where the diff...

journal_title：Biotechnology and bioengineering

authors： Hewitt CJ,Nebe-Von Caron G,Axelsson B,McFarlane CM,Nienow AW

更新日期：2000-11-20 00:00:00

abstract：:Surface plasmon resonance (SPR) technique was used to directly detect an intact form of insect pathogen: the baculovirus, Autographa californica multiple nuclear polyhedrosis virus (AcMNPV). An SPR sensor chip with three bio-functional layers was used to detect the intact AcMNPV: amine-reactive crosslinker with a disu...

journal_title：Biotechnology and bioengineering

authors： Baac H,Hajós JP,Lee J,Kim D,Kim SJ,Shuler ML

更新日期：2006-07-05 00:00:00

abstract：:The production of volatile industrial chemicals utilizing metabolically engineered extreme thermophiles offers the potential for processes with simultaneous fermentation and product separation. An excellent target chemical for such a process is acetone (Tb  = 56°C), ideally produced from lignocellulosic biomass. Caldi...

journal_title：Biotechnology and bioengineering

authors： Straub CT,Bing RG,Otten JK,Keller LM,Zeldes BM,Adams MWW,Kelly RM

更新日期：2020-08-08 00:00:00

abstract：:Aggregates of Escherichia coli confined within hollowfiber reactors were either formed in place by culturing cells within the reactors, or were prepared by injecting a cell suspension that had been concentrated by centrifugation. The effective diffusive permeability of an uncharged nonreacting tracer, nitrous oxide, w...

journal_title：Biotechnology and bioengineering

authors： Libicki SB,Salmon PM,Robertson CR

更新日期：1988-06-20 00:00:00

abstract：:Olive oil hydrolysis using Chromobacterium viscosum lipase B in a reversed micellar media was investigated in a membrane reactor. The dynamic evolution of the product concentration both in the concentrate and permeate stream was analyzed using a mechanistic model previously developed by us and further modified in this...

journal_title：Biotechnology and bioengineering

authors： Prazeres DM,Lemos F,Garcia FA,Cabral JM

更新日期：1993-09-05 00:00:00

abstract：:In an attempt to extend and intensify the productive periods of bioprocesses, a self-cleaning tangential filtration device was examined. Built into a special-design bioreactor, its cell retention was evaluated for continuous-flow operation with selected examples of bacteria (Escherichia coli), yeasts (Sacharomyces cer...

journal_title：Biotechnology and bioengineering

authors： Naja G,Volesky B,Schnell A

更新日期：2006-11-05 00:00:00

abstract：:Isothermal microcalorimeters (IMC) are highly sensitive instruments that allow the measurement of heat flow in the microwatt range. Due to their detection of minute thermal heat, IMC techniques have been used in numerous biological applications, including the study of fermentation processes, pharmaceutical development...

journal_title：Biotechnology and bioengineering

authors： Santoro R,Braissant O,Müller B,Wirz D,Daniels AU,Martin I,Wendt D

更新日期：2011-12-01 00:00:00

abstract：:This article studies the operation of a new process for the production of biopolymers (polyhydroxyalkanoates, PHAs) at different applied organic load rates (OLRs). The process is based on the aerobic enrichment of activated sludge to obtain mixed cultures able to store PHAs at high rates and yields. A mixture of aceti...

journal_title：Biotechnology and bioengineering

authors： Dionisi D,Majone M,Vallini G,Di Gregorio S,Beccari M

更新日期：2006-01-05 00:00:00

abstract：:The activities of lipase from Candida cylindracea and Rhizopus delemar have been investigated in water/AOT/iso-octane reverse micellar media through the use of two esterification reactions: fatty acid-alcohol esterification and glyceride synthesis. Such media promotes the occurrence of these two lipase-catalyzed react...

journal_title：Biotechnology and bioengineering

authors： Hayes DG,Gulari E

更新日期：1990-04-05 00:00:00

abstract：:The immobilization of dextransucrase in Ca-alginate beads relies on the close association between dextran polymer and dextransucrase. However, high amounts of dextran in the enzyme preparation drastically limit the specific activity of the immobilized enzyme (4 U/mL of alginate beads). Moreover, even in the absence of...

journal_title：Biotechnology and bioengineering

authors： Dols-Lafargue M,Willemot RM,Monsan PF,Remaud-Simeon M

更新日期：2001-11-05 00:00:00

abstract：:Biosensors with animal and microbial cells immobilized close to the tip of a membrane electrode have been developed for chemical and drug testing. Our experimental results show that biosensors can be used for drug screening and to provide useful information about various cell-chemical interactions. A computer aided an...

journal_title：Biotechnology and bioengineering

authors： Li XM,Liang BS,Wang HY

更新日期：1988-02-20 00:00:00

abstract：:Fed-batch culture of Bacillus thuringiensis in a modified airlift reactor has been developed by using adaptive control of glucose concentration in the reactor. The glucose concentration was estimated via a correlation equation between carbon dioxide production rate and glucose consumption rate. The estimated glucose c...

journal_title：Biotechnology and bioengineering

authors： Jong JZ,Hsiun DY,Wu WT,Tzeng YM

更新日期：1995-11-05 00:00:00

abstract：:Trehalase is the enzyme which hydrolyzes the disaccharide trehalose into two alpha-D-glucose molecules. In this article, we present the immobilization of trehalase on aminopropyl glass particles. The enzyme was extracted from Escherichia coli Mph2, a strain harboring the pTRE11 plasmid, which contains the trehalase ge...

journal_title：Biotechnology and bioengineering

authors： Bachinski N,Martins AS,Paschoalin VM,Panek AD,Paiva CL

更新日期：1997-04-05 00:00:00

abstract：:The accumulation of inorganic phosphate inhibits protein synthesis in cell-free protein synthesis reactions that are energized by high-energy-phosphate-containing compounds. This study developed a new scheme for supplying energy using dual energy sources to enhance the regeneration of ATP and lower the rate of phospha...

journal_title：Biotechnology and bioengineering

authors： Kim TW,Oh IS,Keum JW,Kwon YC,Byun JY,Lee KH,Choi CY,Kim DM

更新日期：2007-08-15 00:00:00

abstract：:Pretreatment of rice straw by using renewable cholinium amino acids ionic liquids ([Ch][AA] ILs)-water mixtures and the subsequent enzymatic hydrolysis of the residues were conducted in the present work. Of the eight mixtures composed of ILs and water, most were found to be effective for rice straw pretreatment. After...

journal_title：Biotechnology and bioengineering

authors： Hou XD,Li N,Zong MH

更新日期：2013-07-01 00:00:00

abstract：:The goal of drug delivery is to improve the safety and therapeutic efficacy of drugs. This review focuses on delivery platforms that are either derived from endogenous pathways, long-circulating biomolecules and cells or that piggyback onto long-circulating biomolecules and cells. The first class of such platforms is ...

journal_title：Biotechnology and bioengineering

authors： Yousefpour P,Chilkoti A

更新日期：2014-09-01 00:00:00

abstract：:Liquid membrane bilayers, generated by bacteriorhodopsin on a supporting membrane, exhibit photo osmosis. The phenomenon has been shown to be a consequence of light-induced electrical potential differences which develop across the liquid membrane bilayer due to the light-driven proton pumping action of bacteriorhodops...

journal_title：Biotechnology and bioengineering

authors： Srivastava RC,Tandon A

更新日期：1988-04-20 00:00:00

abstract：:A new high throughput technique for enzyme characterization with specific attention to the long term stability, called "Enzyme Test Bench," is presented. The concept of the Enzyme Test Bench consists of short term enzyme tests in 96-well microtiter plates under partly extreme conditions to predict the enzyme long term...

journal_title：Biotechnology and bioengineering

authors： Rachinskiy K,Schultze H,Boy M,Bornscheuer U,Büchs J

更新日期：2009-06-01 00:00:00

abstract：:It is known that the rolA, rolB, and rolC genes of Agrobacterium rhizogenes T-DNA affect processes of plant development and activate the synthesis of secondary metabolites in transformed plant cells. Although a synergistic activity of the rol genes on root formation is well-documented, little is known about their indi...

journal_title：Biotechnology and bioengineering

authors： Shkryl YN,Veremeichik GN,Bulgakov VP,Tchernoded GK,Mischenko NP,Fedoreyev SA,Zhuravlev YN

更新日期：2008-05-01 00:00:00

abstract：:Enterokinase and recombinant enterokinase light chain (rEK(L)) have been used widely to cleave fusion proteins with the target sequence of (Asp)(4)-Lys. In this work, we show that their utility as a site-specific cleavage agent is compromised by sporadic cleavage at other sites, albeit at low levels. Further degradati...

journal_title：Biotechnology and bioengineering

authors： Choi SI,Song HW,Moon JW,Seong BL

更新日期：2001-12-20 00:00:00

abstract：:A new type of liquid emulsion membrane containing reversed micelles for protein extraction is introduced. A three-step extraction mechanism is proposed including solubilization, transportation, and release of the protein. The surfactants Span80 and sodium di(2-ethylhexyl)sulfosuccinate (AOT) are used to stabilize the ...

journal_title：Biotechnology and bioengineering

authors： Stobbe H,Yunguang X,Zihao W,Jufu F

更新日期：1997-02-05 00:00:00

abstract：:The kinetic behavior of soluble and insoluble forms of dextransucrase from Leuconostoc mesenteroides NRRL B-1299 was investigated with sucrose as substrate and maltose as acceptor. To study the parameters involved, a kinetic model was applied that was previously developed for L. mesenteroides NRRL B-512F dextransucras...

journal_title：Biotechnology and bioengineering

authors： Dols M,Remaud-Simeon M,Willemot RM,Demuth B,Jordening HJ,Buchholz K,Monsan P

更新日期：1999-05-05 00:00:00

abstract：:Two empirical models for the enzymatic hydrolysis of cellulose are used to analyze the same set of experimental data to determine if one model is superior to the other. Both models adequately describe the data. The parameters in both models may be correlated to the hydrolysis conditions of enzyme, substrate, and produ...

journal_title：Biotechnology and bioengineering

authors： Holtzapple MT,Caram HS,Humphrey AE

更新日期：1984-08-01 00:00:00

abstract：:During continuous lipase-catalyzed oleic acid esterification by ethanol in n-hexane, the oleic acid conversion, initially at 95%, decreases to 20% after 2 h. This decrease is caused by the accumulation of the water produced in the course of the reaction in the packed-bed reactor (PBR). In order to improve the PBR effi...

journal_title：Biotechnology and bioengineering

authors： Colombié S,Tweddell RJ,Condoret JS,Marty A

更新日期：1998-11-05 00:00:00

abstract：:We have produced Bioglass coatings for Orthopedic implants by using a novel coating technique, CoBlast. The two resultant surfaces, designated BG and hydroxyapatite (HA)/BG, were compared with their HA counterpart, OsteoZip in terms of osteoblastic cell attachment, adhesion, proliferation, differentiation, and growth ...

journal_title：Biotechnology and bioengineering

authors： Tan F,Naciri M,Al-Rubeai M

更新日期：2011-02-01 00:00:00

abstract：:Hepatic steatosis is a process of abnormal lipid deposition within the liver cells, often caused by excessive alcohol uptake or obesity. A conventional in vitro model for hepatic steatosis uses a liver cell culture, treated with fatty acids and measures accumulation of lipids within the cells. This model does not reca...

journal_title：Biotechnology and bioengineering

authors： Lee SY,Sung JH

更新日期：2018-11-01 00:00:00

abstract：:In metabolic engineering, systems which allow coordinated control of two metabolic pathways can be useful. We designed two expression systems and demonstrated their application by coordinating glycogen synthesis and degradation. The first expression vector pMSW2 expressed the glycogen synthesis genes in one operon and...

journal_title：Biotechnology and bioengineering

authors： Dedhia N,Chen W,Bailey JE

更新日期：1997-07-20 00:00:00

abstract：:Exposure of Bacillus subtilis to a shear rate of 1,482/s leads to a rapid loss of cell viability after 10 h of growth. Biochemical and molecular evidences provided below strongly suggest that cell death under high shear results from an apoptosis-like process similar to that described in eukaryotes, with activation of ...

journal_title：Biotechnology and bioengineering

authors： Sahoo S,Rao KK,Suraishkumar GK

更新日期：2006-05-05 00:00:00

abstract：:Preparative-scale purification of plasmid DNA has been attempted by diverse methods, including precipitation with solvents, salts, and detergents and chromatography with ion-exchange, reversed-phase, and size-exclusion columns. Chromatographic methods such as hydrophobic interaction chromatography (HIC), reversed phas...

journal_title：Biotechnology and bioengineering

authors： Lander RJ,Winters MA,Meacle FJ,Buckland BC,Lee AL

更新日期：2002-09-30 00:00:00