Metabolically engineered Caldicellulosiruptor bescii as a platform for producing acetone and hydrogen from lignocellulose.

abstract：:Gene deletion strategies using flux balance analysis (FBA) have improved the growth-coupled production of various compounds. However, the productivities were often below the expectation because the cells failed to adapt to these genetic perturbations. Here, we demonstrate the productivity of the succinate of the desig...

journal_title：Biotechnology and bioengineering

authors： Tokuyama K,Toya Y,Horinouchi T,Furusawa C,Matsuda F,Shimizu H

更新日期：2018-06-01 00:00:00

abstract：:Recent environmental concerns have prompted a re-evaluation of conventional management strategies and refueled the search of innovative waste management practices. In this sense, the anaerobic digestion of both fat and the remaining complex organic matter present in dairy wastewaters is attractive, although the contin...

journal_title：Biotechnology and bioengineering

authors： Coelho NM,Rodrigues AA,Arroja LM,Capela IF

更新日期：2007-02-01 00:00:00

abstract：:L-Threonine, a kind of essential amino acid, has numerous applications in food, pharmaceutical, and aquaculture industries. Fermentative l-threonine production from glucose has been achieved in Escherichia coli. However, there are still several limiting factors hindering further improvement of l-threonine productivity...

journal_title：Biotechnology and bioengineering

authors： Liu J,Li H,Xiong H,Xie X,Chen N,Zhao G,Caiyin Q,Zhu H,Qiao J

更新日期：2019-01-01 00:00:00

abstract：:Remarkable increases in enzyme catalytic stability resulting from addition of charged water-soluble polymers have recently been reported, suggesting that use of these polymers may be an attractive general strategy for enzyme stabilization. To test the proposed hypothesis that coulombic forces between water-soluble pol...

journal_title：Biotechnology and bioengineering

authors： Foreman TM,Khalil M,Meier P,Brainard JR,Vanderberg LA,Sauer NN

更新日期：2001-11-01 00:00:00

abstract：:Three cell lines, that is, the human breast cancer cell line (MCF-7) and the human mammary epithelial cell line (S-1) and its malignant form (T4-2) were embedded in a reconstituted basement membrane (Matrigel) that had 20-nL pyramid-shaped silicon microstructures. The proliferative behavior of the MCF-7 cells was depe...

journal_title：Biotechnology and bioengineering

authors： Torisawa YS,Nashimoto Y,Yasukawa T,Shiku H,Matsue T

更新日期：2007-06-15 00:00:00

abstract：:Corynebacterium hydrocarboclastus KY 8835 grew in the acetate medium and accumulated 28mM Corynecins which was the highest production yield among the processes using various carbon sources. Selective production of Corynecin I (over 90% of all Corynecins), which had been desired for increase of the product yield, was a...

journal_title：Biotechnology and bioengineering

authors： Nakano H,Tomita F,Yamaguchi K,Nagashima M,Suzuki T

更新日期：1977-07-01 00:00:00

abstract：:Cost-effective production of biopharmaceuticals on a large scale can be carried out by perfusion cultures of mammalian cells. One problem with this mode of operation for submerged free-cell cultures is the requirement for an efficient cell separation device located in the effluent stream. The present work investigates...

journal_title：Biotechnology and bioengineering

authors： Docoslis A,Kalogerakis N,Behie LA,Kaler KV

更新日期：1997-05-05 00:00:00

abstract：:Metastatic disease remains one of the primary reasons for cancer-related deaths, yet the majority of in vitro cancer models focus on the primary tumor sites. Here, we describe a metastasis-on-a-chip device that houses multiple bioengineered three-dimensional (3D) organoids, established by a 3D photopatterning techniqu...

journal_title：Biotechnology and bioengineering

authors： Aleman J,Skardal A

更新日期：2019-04-01 00:00:00

abstract：:A limited life cycle assessment (LCA) was performed on a combined biological and chemical process for the production of adipic acid, which was compared to the traditional petrochemical process. The LCA comprises the biological conversion of the aromatic feedstocks benzoic acid, impure aromatics, toluene, or phenol fro...

journal_title：Biotechnology and bioengineering

authors： van Duuren JB,Brehmer B,Mars AE,Eggink G,Dos Santos VA,Sanders JP

更新日期：2011-06-01 00:00:00

abstract：:Protein switches have a variety of potential applications in biotechnology and medicine that motivate efforts to accelerate their development. Switches can be built by the proper fusion of two proteins with the prerequisite input and output functions. However, the exact fusion geometry for switch creation, which typic...

journal_title：Biotechnology and bioengineering

authors： Tullman J,Nicholes N,Dumont MR,Ribeiro LF,Ostermeier M

更新日期：2016-04-01 00:00:00

abstract：:Cross-linked polyurethane (PU) was prepared for entrapping thermolysin. Using the immobilized thermolysin (IT), Z-L-aspartic acid (ZA) was reacted with -Lphenylalanine methyl ester (L-PM) in water-saturated ethyl acetate to give only alpha-Z-L-aspartylL-phenylalanine methyl ester (alpha-ZAPM). Ninety-four percent conv...

journal_title：Biotechnology and bioengineering

authors： Yang CP,Su CS

更新日期：1988-08-20 00:00:00

abstract：:Polyhydroxyalkanoates (PHA) terployesters P(3HB-co-3HV-co-3HHx) consisting of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxyhexanoate (3HHx) were produced by wild-type Aeromonas hydrophila 4AK4, its recombinant harboring PHA synthesis genes phaPCJ encoding PHA binding protein phasin, PHA synthase, and...

journal_title：Biotechnology and bioengineering

authors： Zhang HF,Ma L,Wang ZH,Chen GQ

更新日期：2009-10-15 00:00:00

abstract：:As the therapeutic monoclonal antibody (mAb) market continues to grow, optimizing production processes is becoming more critical in improving efficiencies and reducing cost-of-goods in large-scale production. With the recent trends of increasing cell culture titers from upstream process improvements, downstream capaci...

journal_title：Biotechnology and bioengineering

authors： Dizon-Maspat J,Bourret J,D'Agostini A,Li F

更新日期：2012-04-01 00:00:00

abstract：:The marine diatom Cyclotella cryptica was grown over a period of 13 months in a 48-m(2) shallow outdoor flume. The use of foil arrays at intervals of 1.2 m to effect systematic vertical mixing in the flume was found to significantly enhance microalgal production (p = 0.006). Average photosynthetic efficiencies (based ...

journal_title：Biotechnology and bioengineering

authors： Laws EA,Taguchi S,Hirata J,Pang L

更新日期：1988-07-05 00:00:00

abstract：:Studies have been conducted in a gas circulation type fermentor in order to characterize the ethanol fermentation of uncooked cassava starch with Rhizopus koji. Results showed that ethanol concentration reached 13-14% (v/v) in 4-day broth, and the maximum productivity of ethanol was 2.3 g ethanol/L broth h. This produ...

journal_title：Biotechnology and bioengineering

authors： Fujio Y,Ogata M,Ueda S

更新日期：1985-08-01 00:00:00

abstract：:Microaerobic (oxygen-limited) conditions are critical for inducing many important microbial processes in industrial or environmental applications. At very low oxygen concentrations, however, the process performance often suffers from technical limitations. Available dissolved oxygen measurement techniques are not sens...

journal_title：Biotechnology and bioengineering

authors： Carius L,Rumschinski P,Faulwasser T,Flockerzi D,Grammel H,Findeisen R

更新日期：2014-04-01 00:00:00

abstract：:The investigation of new adjuvants is essential for the development of efficacious vaccines. Chitosan (CS), a derivative of chitin, has been shown to act as an adjuvant, improving vaccine-induced immune responses. However, the effect of CS molecular weight (MW) on this adjuvanticity has not been investigated, despite ...

journal_title：Biotechnology and bioengineering

authors： Lampe AT,Farris EJ,Brown DM,Pannier AK

更新日期：2020-12-07 00:00:00

abstract：:Numerous high-value proteins are secreted into the Escherichia coli periplasm by the General Secretory (Sec) pathway, but Sec-based production chassis cannot handle many potential target proteins. The Tat pathway offers a promising alternative because it transports fully folded proteins; however, yields have been too ...

journal_title：Biotechnology and bioengineering

authors： Browning DF,Richards KL,Peswani AR,Roobol J,Busby SJW,Robinson C

更新日期：2017-12-01 00:00:00

abstract：:A fuzzy-logic light attenuation model was developed and validated for a stirred-tank photobioreactor. Based on this model, local light intensities were used to calculate local specific growth rates of the cyanobacteria Synechococcus PCC 7942. The light regime for maximization of biomass space-time yield in a batch pro...

journal_title：Biotechnology and bioengineering

authors： Franco-Lara E,Havel J,Peterat F,Weuster-Botz D

更新日期：2006-12-20 00:00:00

abstract：:Cytidine-5'-diphosphocholine (CDP-choline) is a widely used neuroprotective drug for multiple indications. In industry, CDP-choline is synthesized by a two-step cell culture/permeabilized cell biotransformation method because substrates often do not enter cells in an efficient manner. This study develops a novel one-s...

journal_title：Biotechnology and bioengineering

authors： Ren Y,Liu Q,Liu H,Zhou X,Zhang Y,Cai M

更新日期：2020-05-01 00:00:00

abstract：:Acidic Golgi pH plays an important role in protein glycosylation, one of the critical quality attributes of therapeutic proteins. To determine the intracellular Golgi pH during culture, stable Chinese hamster ovary (CHO) cell clones expressing pHluorin2, a ratiometric pH-sensitive fluorescent protein (FP), in the cis-...

journal_title：Biotechnology and bioengineering

authors： Lee JH,Kim J,Park JH,Heo WD,Lee GM

更新日期：2019-05-01 00:00:00

abstract：:This study demonstrates that masking the Feb regions of a monoclonal antibody (Mab) with synthetic antigens prior to covalent immobilization efficiency. Water-soluble adducts of poly(2-methyloxazoline) polymers and a synthetic-peptide epitope for the Mab were constructed. These synthetic antigens are referred to as Fa...

journal_title：Biotechnology and bioengineering

authors： Velander WH,Subramanian A,Madurawe RD,Orthner CL

更新日期：1992-04-25 00:00:00

abstract：:The initial steps of glycerol dissimilation and 1,3-propanediol (1, 3-PD) formation by Klebsiella pneumoniae anaerobically grown on glycerol were studied by quantifying the in vitro and in vivo activities of enzymes in continuous culture under conditions of steady state and oscillation and during transient phases. The...

journal_title：Biotechnology and bioengineering

authors： Ahrens K,Menzel K,Zeng A,Deckwer W

更新日期：1998-09-05 00:00:00

abstract：:The possibility of enhancing the biomass productivity of a continuous culture of Saccharomyces cerevisiae growing on a glucose-limited medium is addressed. An unstructured Monod-type model is first identified using steady-state data. The culture is subjected to step changes in dilution rate, and it is seen that the Mo...

journal_title：Biotechnology and bioengineering

authors： Abulesz EM,Lyberatos G

更新日期：1989-09-01 00:00:00

abstract：:A mathematical model is developed to analyze the influence of chemical reaction and diffusion processes on the intracellular organization of mitochondria in skeletal muscle cells. The mathematical modeling approach uses a reaction-diffusion analysis of oxygen, ATP, and ADP involved in energy metabolism and mitochondri...

journal_title：Biotechnology and bioengineering

authors： Pathi B,Kinsey ST,Locke BR

更新日期：2011-08-01 00:00:00

abstract：:Production of conidia of the biocontrol fungus Coniothyrium minitans by solid-state cultivation in a packed-bed reactor on an industrial scale is feasible. Spore yield and oxygen consumption rate of C. minitans during cultivation on oats and three inert solids (hemp, perlite, and bagasse) saturated with a liquid mediu...

journal_title：Biotechnology and bioengineering

authors： Weber FJ,Tramper J,Rinzema A

更新日期：1999-11-20 00:00:00

abstract：:Electron paramagnetic resonance spectroscopy is used to observe hydroxyl radicals produced by an atmospheric pressure nonthermal plasma device at distances greater than 1 m from the discharge. The plasma device is an indirect treatment setup with closed loop airflow and hydrogen peroxide additives that is effective in...

journal_title：Biotechnology and bioengineering

authors： Plimpton SR,Gołkowski M,Mitchell DG,Austin C,Eaton SS,Eaton GR,Gołkowski C,Voskuil M

更新日期：2013-07-01 00:00:00

abstract：:Fast and efficient production of recombinant proteins for structural and functional studies is a crucial issue for research and for industry. To this end, we have developed an efficient system to generate in less than 2 months, starting from the cDNA, pools of CHO cells stably expressing high-level of recombinant prot...

journal_title：Biotechnology and bioengineering

authors： Gaillet B,Gilbert R,Broussau S,Pilotte A,Malenfant F,Mullick A,Garnier A,Massie B

更新日期：2010-06-01 00:00:00

abstract：:A simple feeding strategy was developed and successfully employed for nutritional control in a 2-L fed-batch culture of hybridoma cells. A previously developed stoichiometric model for animal cell growth was used to design a supplemental medium for feeding. Undialyzed fetal bovine serum and trace metals (Fe(2+), SeO(3...

journal_title：Biotechnology and bioengineering

authors： Xie L,Wang DI

更新日期：1996-09-20 00:00:00

abstract：:Both k(L)a and k(L) measurements were carried out by an unsteady state technique at impeller speeds ranging from 1.6 to 5.8 s(-1) in a mechanically agitated animal cell culture vessel of working volume 1.5 L. Checks were made that the time constant of the oxygen electrode was negligible compared to the time for aerati...

journal_title：Biotechnology and bioengineering

authors： Lavery M,Nienow AW

更新日期：1987-08-20 00:00:00