Antibody-based surface plasmon resonance detection of intact viral pathogen.

abstract：:The impact of process environment changes on process performance is one of the most crucial process safety issues when cultivating mammalian cells in a bioreactor. In contrast, directed shifting of process parameters can also be used as an optimization tool providing higher cell and product yields. Compared to other s...

journal_title：Biotechnology and bioengineering

authors： Trummer E,Fauland K,Seidinger S,Schriebl K,Lattenmayer C,Kunert R,Vorauer-Uhl K,Weik R,Borth N,Katinger H,Müller D

更新日期：2006-08-20 00:00:00

abstract：:A respiratory-deficient, mutant of Kluyveromyces fragilis was isolated using a ethidium bromide mutagenesis. It was characterized by a loss of cytochromes a + a3 and by an improvement of its inulinase activity. Under anaerobic conditions this mutant was always better than the wild strain for ethanol production especia...

journal_title：Biotechnology and bioengineering

authors： Guiraud JP,Bourgi J,Stervinou M,Claisse M,Galzy P

更新日期：1987-05-01 00:00:00

abstract：:Biopharmaceutical production would benefit from rapid methods to optimize production of therapeutic proteins by screening host cell line/vector combination, culture media, and operational parameters such as timing of induction. Miniaturized bioreactors are an emerging research area aiming at improving the development ...

journal_title：Biotechnology and bioengineering

authors： Diao J,Young L,Zhou P,Shuler ML

更新日期：2008-05-01 00:00:00

abstract：:A kinetic model describing the cephalosporin biosynthesis in Streptomyces clavuligerus was developed. Using previously reported kinetic data of biosynthetic enzymes, we examined the kinetics of cephalosporin production. The predicted time profile of the specific production rate during a batch culture parallels that of...

journal_title：Biotechnology and bioengineering

authors： Malmberg LH,Hu WS

更新日期：1991-10-20 00:00:00

abstract：:Recombinant Escherichia coli harboring the medium-chain-length (MCL) polyhydroxyalkanoate (PHA) synthase gene has been shown to accumulate MCL-PHAs from fatty acids when FadB is inactive. However, the enzymes in fadB mutant E. coli responsible for channeling the beta-oxidation intermediates to PHA biosynthesis have no...

journal_title：Biotechnology and bioengineering

authors： Park SJ,Yup Lee S

更新日期：2004-06-20 00:00:00

abstract：:In this study, a non-sterile (open) continuous fermentation (OCF) process with no-carbon loss was developed to improve lactic acid (LA) productivity and operational stability from the co-utilization of lignocellulose-derived sugars by thermophilic Enterococcus faecium QU 50. The effects of different sugar mixtures on ...

journal_title：Biotechnology and bioengineering

authors： Abdel-Rahman MA,Tan J,Tashiro Y,Zendo T,Sakai K,Sonomoto K

更新日期：2020-06-01 00:00:00

abstract：:RNA sequencing (RNASeq) has been widely used to associate alterations in Chinese hamster ovary (CHO) cell gene expression with bioprocess phenotypes; however, alternative messenger RNA (mRNA) splicing, has thus far, received little attention. In this study, we utilized RNASeq for transcriptomic analysis of a monoclona...

journal_title：Biotechnology and bioengineering

authors： Tzani I,Monger C,Motheramgari K,Gallagher C,Hagan R,Kelly P,Costello A,Meiller J,Floris P,Zhang L,Clynes M,Bones J,Barron N,Clarke C

更新日期：2020-08-01 00:00:00

abstract：:In crude extract-based cell-free protein synthesis (CFPS), DNA templates are transcribed and translated into functional proteins. Although linear expression templates (LETs) are less laborious and expensive to generate, plasmid templates are often desired over polymerase chain reaction-generated LETs due to increased ...

journal_title：Biotechnology and bioengineering

authors： Zhu B,Gan R,Cabezas MD,Kojima T,Nicol R,Jewett MC,Nakano H

更新日期：2020-08-20 00:00:00

abstract：:Genome-scale flux analysis of Escherichia coli DH5alpha growth in a complex medium was performed to investigate the relationship between the uptake of various nutrients and their metabolic outcomes. During the exponential growth phase, we observed a sequential consumption order of serine, aspartate and glutamate in th...

journal_title：Biotechnology and bioengineering

authors： Selvarasu S,Ow DS,Lee SY,Lee MM,Oh SK,Karimi IA,Lee DY

更新日期：2009-02-15 00:00:00

abstract：:We have produced Bioglass coatings for Orthopedic implants by using a novel coating technique, CoBlast. The two resultant surfaces, designated BG and hydroxyapatite (HA)/BG, were compared with their HA counterpart, OsteoZip in terms of osteoblastic cell attachment, adhesion, proliferation, differentiation, and growth ...

journal_title：Biotechnology and bioengineering

authors： Tan F,Naciri M,Al-Rubeai M

更新日期：2011-02-01 00:00:00

abstract：:The study reports on the development of a bioreactor for the production of alpha-keto acids from D,L- or D-amino acids using Rhodotorula gracilis D-amino acid oxidase. D-amino acid oxidase was co-immobilized with catalase on Affi-Gel 10 matrix, and the reactor was operated as a continuous-stirred tank reactor (CSTR) o...

journal_title：Biotechnology and bioengineering

authors： Butò S,Pollegioni L,D'Angiuro L,Pilone MS

更新日期：1994-12-01 00:00:00

abstract：:Pathway refactoring serves as an invaluable synthetic biology tool for natural product discovery, characterization, and engineering. However, the complicated and laborious molecular biology techniques largely hinder its application in natural product research, especially in a high-throughput manner. Here we report a p...

journal_title：Biotechnology and bioengineering

authors： Ren H,Hu P,Zhao H

更新日期：2017-08-01 00:00:00

abstract：:Soluble steel factor (SF) is a potent stimulator of hematopoietic progenitor cell proliferation in vitro, and cytokine combinations that include SF can support extensive expansions of hematopoietic cells. Recently, we showed that very primitive progenitor cells from normal human bone marrow require exposure to very hi...

journal_title：Biotechnology and bioengineering

authors： Zandstra PW,Jervis E,Haynes CA,Kilburn DG,Eaves CJ,Piret JM

更新日期：1999-05-20 00:00:00

abstract：:Direct perfusion of 3D tissue engineered constructs is known to enhance osteogenesis, which can be partly attributed to enhanced nutrient and waste transport. In addition flow mediated shear stresses are known to upregulate osteogenic differentiation and mineralization. A quantification of the hydrodynamic environment...

journal_title：Biotechnology and bioengineering

authors： Maes F,Van Ransbeeck P,Van Oosterwyck H,Verdonck P

更新日期：2009-06-15 00:00:00

abstract：:Engineered strains of Saccharomyces cerevisiae are used for industrial production of succinic acid. Optimal process conditions for dicarboxylic-acid yield and recovery include slow growth, low pH, and high CO2 . To quantify and understand how these process parameters affect yeast physiology, this study investigates in...

journal_title：Biotechnology and bioengineering

authors： Hakkaart X,Liu Y,Hulst M,El Masoudi A,Peuscher E,Pronk J,van Gulik W,Daran-Lapujade P

更新日期：2020-03-01 00:00:00

abstract：:Novel amphoteric particles are prepared by an interfacial polyaddition reaction of ethylene glycol diglycidyl ether (EGDGE) and L-lysine in a water-in-oil (W/O) miniemulsion system. The zeta-potential of the copolymer particle as a function of pH indicates a sigmoid curve with an isoelectric point at pH 8.7 due to the...

journal_title：Biotechnology and bioengineering

authors： Taira S,Du YZ,Kodaka M

更新日期：2006-02-05 00:00:00

abstract：:While feeding protocols and ectopic expression of anti-apoptotic genes have been used to improve the viability of hybridoma cell lines, the effect of the expression levels of survival genes on the behavior of hybridomas following nutrient supplementation is unknown. In this study, we compared the behavior of the Sp2/0...

journal_title：Biotechnology and bioengineering

authors： Charbonneau JR,Furtak T,Lefebvre J,Gauthier ER

更新日期：2003-02-05 00:00:00

abstract：:This article describes a method for the chemical immobilization of concanavalin A (Con A) on the inside wall of a single hollow cellulose fiber for use in glucose affinity sensor. Periodate oxidation of cellulose fiber followed by a spacer for Con A attachment was deemed to be the most optimal procedure for achieving ...

journal_title：Biotechnology and bioengineering

authors： Srinivasan KR,Mansouri S,Schultz JS

更新日期：1986-02-01 00:00:00

abstract：:Two metabolic engineering tools, namely gene inactivation and gene overexpression, were employed to examine the effects of two genetic modifications on the fermentation characteristics of Clostridium acetobutylicum. Inactivation of the butyrate kinase gene (buk) was examined using strain PJC4BK, while the combined eff...

journal_title：Biotechnology and bioengineering

authors： Harris LM,Desai RP,Welker NE,Papoutsakis ET

更新日期：2000-01-05 00:00:00

abstract：:The sufficient provision of oxygen is mandatory for enzymatic oxidations in aqueous solution, however, in process optimization this still is a bottleneck that cannot be overcome with the established methods of macrobubble aeration. Providing higher mass transfer performance through microbubble aerators, inefficient ae...

journal_title：Biotechnology and bioengineering

authors： Thomas B,Ohde D,Matthes S,Engelmann C,Bubenheim P,Terasaka K,Schlüter M,Liese A

更新日期：2021-01-01 00:00:00

abstract：:The increasing accessibility of mass isotopomer data via GC-MS and NMR technology has necessitated the use of a systematic and reliable method to take advantage of such data for flux analysis. Here we applied a nonlinear, optimization-based method to study substrate metabolism in cardiomyocytes using (13)C data from p...

journal_title：Biotechnology and bioengineering

authors： Vo TD,Palsson BO

更新日期：2006-12-05 00:00:00

abstract：:Two kinds of disposable bioreactors, air-lift disposable bioreactors (ADB) and wave disposable bioreactors (WDB) were compared with stirred-tank reactors (5-L STR). These bioreactors were successfully applied to transgenic rice cell cultures for the production of recombinant human cytotoxic T-lymphocyte antigen 4-immu...

journal_title：Biotechnology and bioengineering

authors： Kwon JY,Yang YS,Cheon SH,Nam HJ,Jin GH,Kim DI

更新日期：2013-09-01 00:00:00

abstract：:We propose an in vitro selection strategy to identify bacteriophage variants that recognize metal ions in solution. In 6 M urea, phage T7 loses 99.9% of its activity in less than 5 min. Inactivation is accelerated by gold, but slowed by zinc and magnesium. Selection of phage over five generations in the presence of go...

journal_title：Biotechnology and bioengineering

authors： Gupta K,Yin J

更新日期：1995-03-05 00:00:00

abstract：:A continuous viral inactivation (CVI) chamber has been designed to operate with acceptable residence time distribution (RTD) characteristics. However, altering the CVI's geometry and operation to accommodate the scale was not obvious. In this work, we elucidate the influence of Dean vortices and leverage the transitio...

journal_title：Biotechnology and bioengineering

authors： Brown MR,Orozco R,Coffman J

更新日期：2020-03-01 00:00:00

abstract：:The mobility of water in intact biofilms was measured with pulsed field gradient nuclear magnetic resonance (PFG-NMR) and used to characterise their diffusive properties. The results obtained with several well-defined systems, viz. pure water, agar, and agar containing inert particles or active bacteria were compared ...

journal_title：Biotechnology and bioengineering

authors： Beuling EE,van Dusschoten D,Lens P,van den Heuvel JC,Van As H,Ottengraf SP

更新日期：1998-11-05 00:00:00

abstract：:In vitro engineering systems can be powerful tools for studying tissue development in response to biophysical stimuli as well as for evaluating the functionality of engineered tissue grafts. It has been challenging, however, to develop systems that adequately integrate the application of biomimetic mechanical strain t...

journal_title：Biotechnology and bioengineering

authors： Cook CA,Huri PY,Ginn BP,Gilbert-Honick J,Somers SM,Temple JP,Mao HQ,Grayson WL

更新日期：2016-08-01 00:00:00

abstract：:Due to its availability, low-price, and high degree of reduction, glycerol has become an attractive carbon source for the production of fuels and reduced chemicals. Using the platform we have established from the identification of key pathways mediating fermentative metabolism of glycerol, this work reports the engine...

journal_title：Biotechnology and bioengineering

authors： Clomburg JM,Gonzalez R

更新日期：2011-04-01 00:00:00

abstract：:A model that portrays substrate profiles in a steady-state multispecies granular biofilm is developed and coupled with a biofilm detachment model. The model accounts for glucose, propionate, hydrogen, and acetate transformations performed by three bacterial trophic groups: acidogens, syntrophic bacterial consortia, an...

journal_title：Biotechnology and bioengineering

authors： Tartakovsky B,Guiot SR

更新日期：1997-04-20 00:00:00

abstract：:Addition of Tween 85 to aqueous suspensions of Anabaena variabilis induced photosynthetic evolution of hydrogen over a time span of several weeks: As much as 148 nmol H(2)/h . mg dry weight was produced in the first week by a suspension containing 4.2 mg dry weight of cells and 77 mM Tween 85. The chemical structure o...

journal_title：Biotechnology and bioengineering

authors： Famiglietti M,Hochkoeppler A,Luisi PL

更新日期：1993-10-01 00:00:00

abstract：:Recent environmental concerns have prompted a re-evaluation of conventional management strategies and refueled the search of innovative waste management practices. In this sense, the anaerobic digestion of both fat and the remaining complex organic matter present in dairy wastewaters is attractive, although the contin...

journal_title：Biotechnology and bioengineering

authors： Coelho NM,Rodrigues AA,Arroja LM,Capela IF

更新日期：2007-02-01 00:00:00