Abstract:
:The mobility of water in intact biofilms was measured with pulsed field gradient nuclear magnetic resonance (PFG-NMR) and used to characterise their diffusive properties. The results obtained with several well-defined systems, viz. pure water, agar, and agar containing inert particles or active bacteria were compared to glucose diffusion coefficients measured with micro-electrodes and those calculated utilising theoretical diffusion models. A good correspondence was observed indicating that PFG-NMR should also enable the measurement of diffusion coefficients in heterogeneous biological systems. Diffusion coefficients of several types of natural biofilms were measured as well and these results were related to the physical biofilm characteristics. The values had a high accuracy and reflected the properties of a sample of ca. 100 biofilms, while non-uniformity or non-geometrical shapes did not negatively influence the results. The monitored PFG-NMR signal contains supplementary information on e.g. cell fraction or spatial organisation but quantitative analysis was not yet possible. Copyright 1998 John Wiley & Sons, Inc.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Beuling EE,van Dusschoten D,Lens P,van den Heuvel JC,Van As H,Ottengraf SPdoi
10.1002/(sici)1097-0290(19981105)60:3<283::aid-bitsubject
Has Abstractpub_date
1998-11-05 00:00:00pages
283-91issue
3eissn
0006-3592issn
1097-0290pii
10.1002/(SICI)1097-0290(19981105)60:3<283::AID-BITjournal_volume
60pub_type
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