Abstract:
:Recent works have pointed to the use of volatile electrolytes such as carbon dioxide (CO2) dissolved in aqueous solutions as a promising alternative to the precipitating agents conventionally used for protein recovery in the food and pharmaceutical industries. In this work we investigated experimental and theoretical aspects of the precipitation of porcine insulin, a biomolecule of pharmaceutical interest, using CO2 as an acid-precipitating agent. The solubility of porcine insulin in NaHCO3 solutions in pressurized CO2 was determined as a function of temperature and pressure, with a minimum being observed close to the protein isoelectric point. A thermodynamic model was developed and successfully utilized to correlate the experimental data. Insulin was considered a polyelectrolyte in the model and its self-association reactions were also taken into account. The biological activity of insulin was maintained after precipitation with CO2, although some activity can be lost if foam is formed in the depressurization step.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Tashima AK,Ottens M,Van der Wielen LA,Cintra DE,Pauli JR,Filho Pde A,Miranda EAdoi
10.1002/bit.22319subject
Has Abstractpub_date
2009-08-01 00:00:00pages
909-19issue
5eissn
0006-3592issn
1097-0290journal_volume
103pub_type
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