Optimization of biomass productivity and substrate utility of a hydrogen bacterium, Alcaligenes hydrogenophilus.

abstract：:Mevalonate is a useful metabolite synthesized from three molecules of acetyl-CoA, consuming two molecules of NADPH. Escherichia coli ( E. coli) catabolizes glucose to acetyl-CoA via several routes, such as the Embden-Meyerhof-Parnas (EMP) and the oxidative pentose phosphate (oxPP) pathways. Although the oxPP pathway s...

journal_title：Biotechnology and bioengineering

authors： Kamata K,Toya Y,Shimizu H

更新日期：2019-05-01 00:00:00

abstract：:Bovine pancreatic trypsin was treated with ethylene glycol bis(succinic acid N-hydroxysuccinimide ester). Approximately 8 of 14 lysines per trypsin molecule were modified. This derivative (EG trypsin) was more stable than native between 30 degrees and 70 degrees C: T50 values were 59 degrees C and 46 degrees C, respec...

journal_title：Biotechnology and bioengineering

authors： Murphy A,O Fágáin C

更新日期：1998-05-20 00:00:00

abstract：:Agility to schedule and execute cell culture manufacturing campaigns quickly in a multi-product facility will play a key role in meeting the growing demand for therapeutic proteins. In an effort to shorten campaign timelines, maximize plant flexibility and resource utilization, we investigated the initiation of cell c...

journal_title：Biotechnology and bioengineering

authors： Seth G,Hamilton RW,Stapp TR,Zheng L,Meier A,Petty K,Leung S,Chary S

更新日期：2013-05-01 00:00:00

abstract：:Innovation in monoclonal antibody (mAb) production continues to be driven by cell engineering strategies to increase yield and improve product quality. In a previous study, to investigate the effectiveness of transporter overexpression strategies, we prepared a taurine transporter-overexpressing host cell line (DXB11/...

journal_title：Biotechnology and bioengineering

authors： Tabuchi H,Sugiyama T

更新日期：2013-08-01 00:00:00

abstract：:Penicillinase (beta-lactamase I, EC 3.5.2.6) secreted by Bacillus cereus, strain 569/H, was covalently attached to aminoethyl cellulose via glutaraldehyde. The immobilized derivative shows increased thermostability and decreased susceptibility to conformational changes induced by certain substrates of penicillinase. T...

journal_title：Biotechnology and bioengineering

authors： Klemes Y,Citri N

更新日期：1979-05-01 00:00:00

abstract：:The microscopic morphology of Trichoderma reesei QM 9414, growing in submerged culture, was studied by image analysis. The morphology was characterized by the total hyphal length, the total number of tips, the number of actively growing tips, and the length of the main hypha. To describe the growth of a single myceliu...

journal_title：Biotechnology and bioengineering

authors： Lejeune R,Nielsen J,Baron GV

更新日期：1995-09-05 00:00:00

abstract：:The influence of biofilm areal cell density, species composition, and the presence of abiotic particles on the disinfection and removal of bacterial biofilms by monochloramine was investigated. Mono- and binary population biofilms of Pseudomonas aeruginosa and Klebsiella pneumoniae were grown on stainless-steel slides...

journal_title：Biotechnology and bioengineering

authors： Srinivasan R,Stewart PS,Griebe T,Chen CI,Xu X

更新日期：1995-06-20 00:00:00

abstract：:Macroencapsulation technology has been an attractive topic in the field of treatment for Type 1 diabetes due to mechanical stability, versatility, and retrievability of the macro-capsule design. Macro-capsules can be categorized into extravascular and intravascular devices, in which solute transport relies either on d...

journal_title：Biotechnology and bioengineering

authors： Song S,Roy S

更新日期：2016-07-01 00:00:00

abstract：:The efficiency of a versatile in vivo cascade involving a promiscuous alcohol dehydrogenase, obtained from a biodiversity search, and a Baeyer-Villiger monooxygenase was enhanced by the independent control of the production level of each enzyme to produce ε-caprolactone and 3,4-dihydrocoumarin. This goal was achieved ...

journal_title：Biotechnology and bioengineering

authors： Ménil S,Petit JL,Courvoisier-Dezord E,Debard A,Pellouin V,Reignier T,Sergent M,Deyris V,Duquesne K,de Berardinis V,Alphand V

更新日期：2019-11-01 00:00:00

abstract：:This study deals with the use of an upward gas/liquid slug flow to reduce tubular mineral membrane fouling. The injection of air into the feedstream is designed to create hydrodynamic conditions that destabilize the cake layer over the membrane surface inside the filtration module complex. Experimental study was carri...

journal_title：Biotechnology and bioengineering

authors： Mercier M,Maranges C,Fonade C,Lafforgue-Delorme C

更新日期：1998-04-05 00:00:00

abstract：:We performed a macrokinetic and quantitative microbial investigation of a continuously operating bench-scale biofilter treating styrene-polluted gases. The device was filled with a mixture of peat and glass beads as packing medium and inoculated with the styrene-oxidizing strain, Rhodococcus rhodochrous AL NCIMB 13259...

journal_title：Biotechnology and bioengineering

authors： Zilli M,Converti A,Di Felice R

更新日期：2003-07-05 00:00:00

abstract：:The cellulase production from immobilized Trichoderma reesei composites prepared by radiation polymerization at low temperature was studied. The production of cellulase from the cells irradiated by radiation was slightly retarded at the initial stage of the culture, but was immediately recovered. The production of cel...

journal_title：Biotechnology and bioengineering

authors： Kumakura M,Kaetsu I,Nisizawa K

更新日期：1984-01-01 00:00:00

abstract：:Glycosylation is a key critical quality attribute for monoclonal antibodies and other recombinant proteins because of its impact on effector mechanisms and half-life. In this study, a variety of compounds were evaluated for their ability to modulate glycosylation profiles of recombinant monoclonal antibodies produced ...

journal_title：Biotechnology and bioengineering

authors： Ehret J,Zimmermann M,Eichhorn T,Zimmer A

更新日期：2019-04-01 00:00:00

abstract：:Lutein and zeaxanthin are dietary carotenoids reported to be protective against age-related macular degeneration. Recently, the green alga Chlamydomonas reinhardtii has received attention as a photosynthetic cell factory, but the potential of this alga for carotenoid production has not yet been evaluated. In this stud...

journal_title：Biotechnology and bioengineering

authors： Baek K,Yu J,Jeong J,Sim SJ,Bae S,Jin E

更新日期：2018-03-01 00:00:00

abstract：:Murine hybridoma cells were grown in a bubble column that was inclined up to 45 degrees from vertical. Inclining the column by a few degrees separated the rising bubbles against the upper surface, leaving the bulk of the liquid bubble free. The liquid was circulated well by the rising bubbles, but collection of cells ...

journal_title：Biotechnology and bioengineering

authors： Lu GZ,Gray MR,Thompson BG,Suresh MR

更新日期：1995-01-20 00:00:00

abstract：:Intracellular delivery of functional proteins is of great interest for basic biological research as well as for clinical applications. Transfection is the most commonly used method, however, it is not applicable to large-scale manipulation and inefficient in important cell types implicated in biomedical applications, ...

journal_title：Biotechnology and bioengineering

authors： Li Z,Cai Z,Fu W,Liu Y,Tian C,Wang H,Fu T,Wu Z,Wu D,Jin Y,Cheng Z,Terada N,Liu L,Wu W,Jin S,Bai F

更新日期：2020-03-01 00:00:00

abstract：:The diffusion of viruses toward cells is a limiting step of the infection process. To be modeled correctly, this step must be evaluated in combination with the adsorption of the virus to the cell surface, which is a rapid but reversible step. In this paper, the recombinant adenovirus (rAd) diffusion and its adsorption...

journal_title：Biotechnology and bioengineering

authors： Gilbert PA,Kamen A,Bernier A,Garnier A

更新日期：2007-09-01 00:00:00

abstract：:Abnormal airway mucus presents a significant challenge for inhalational drug delivery. Recognizing the thick and tenacious airway mucus seen in the cystic fibrosis (CF) patients as a critical barrier to effective drug delivery, both into the mucus layer itself as well as across that layer to the underlying airway epit...

journal_title：Biotechnology and bioengineering

authors： Yang Y,Tsifansky MD,Shin S,Lin Q,Yeo Y

更新日期：2011-06-01 00:00:00

abstract：:The relationship between oxygen concentration and growth rate in the yeast Trichosporon cutaneum was studied. In order to establish the conditions for purely oxygen-limited growth, the cells were first grown in a carbon-limited chemostat, and kinetic parameters determined. The cells were then grown in an oxygen-limite...

journal_title：Biotechnology and bioengineering

authors： Käppeli O,Fiechter A

更新日期：1982-11-01 00:00:00

abstract：:The downstream processing of virus particles for vaccination or gene therapy is becoming a critical bottleneck as upstream titers keep improving. Moreover, the growing pressure to develop cost-efficient processes has brought forward new downstream trains. This review aims at analyzing the state-of-the-art in viral dow...

journal_title：Biotechnology and bioengineering

authors： Nestola P,Peixoto C,Silva RR,Alves PM,Mota JP,Carrondo MJ

更新日期：2015-05-01 00:00:00

abstract：:The adsorption of Thiobacillus ferrooxidans to coal surfaces has been studied. Adsorption experiments were conducted on coal samples from eight different Eastern coal fields. In all cases the adsorption process was at least 90% complete within the first two minutes following inoculation. The results of these experimen...

journal_title：Biotechnology and bioengineering

authors： Bagdigian RM,Myerson AS

更新日期：1986-04-01 00:00:00

abstract：:More than 80 years after its discovery, penicillin is still a widely used and commercially highly important antibiotic. Here, we analyse the metabolic network of penicillin synthesis in Penicillium chrysogenum based on the concept of elementary flux modes. In particular, we consider the synthesis of the invariant mole...

journal_title：Biotechnology and bioengineering

authors： Prauße MT,Schäuble S,Guthke R,Schuster S

更新日期：2016-01-01 00:00:00

abstract：:The treatment of large segmental bone defects remains a challenge as infection, delayed union, and nonunion are common postoperative complications. A three-dimensional printed bioresorbable and physiologically load-sustaining graft substitute was developed to mimic native bone tissue for segmental bone repair. Fabrica...

journal_title：Biotechnology and bioengineering

authors： Chung R,Kalyon DM,Yu X,Valdevit A

更新日期：2018-09-01 00:00:00

abstract：:Liquid membrane bilayers, generated by bacteriorhodopsin on a supporting membrane, exhibit photo osmosis. The phenomenon has been shown to be a consequence of light-induced electrical potential differences which develop across the liquid membrane bilayer due to the light-driven proton pumping action of bacteriorhodops...

journal_title：Biotechnology and bioengineering

authors： Srivastava RC,Tandon A

更新日期：1988-04-20 00:00:00

abstract：:Electron paramagnetic resonance spectroscopy is used to observe hydroxyl radicals produced by an atmospheric pressure nonthermal plasma device at distances greater than 1 m from the discharge. The plasma device is an indirect treatment setup with closed loop airflow and hydrogen peroxide additives that is effective in...

journal_title：Biotechnology and bioengineering

authors： Plimpton SR,Gołkowski M,Mitchell DG,Austin C,Eaton SS,Eaton GR,Gołkowski C,Voskuil M

更新日期：2013-07-01 00:00:00

abstract：:Cellulase was modified with amphilic copolymers made of alpha-allyl-omega-methoxy polyoxyalkylene (POA) and maleic acid anhydride (MAA) to improve the cellulose hydrolytic reactivity and cellulase separation. Amino groups of the cellulase molecule are covalently coupled with the MAA functional groups of the copolymer....

journal_title：Biotechnology and bioengineering

authors： Park JW,Kajiuchi T

更新日期：1995-02-20 00:00:00

abstract：:Identification of conditions for guided and specific differentiation of human stem cell and progenitor cells is important for continued development and engineering of in vitro cell culture systems for use in regenerative medicine, drug discovery, and human toxicology. Three-dimensional (3D) and organotypic cell cultur...

journal_title：Biotechnology and bioengineering

authors： Nierode GJ,Gopal S,Kwon P,Clark DS,Schaffer DV,Dordick JS

更新日期：2019-01-01 00:00:00

abstract：:The behavior of 20 beta-hydroxysteroid dehydrogenase in a two-phase system consisting of an emulsion of water with an immiscible organic solvent was investigated. The effect of several organic solvents on the stability, activity, and kinetic profile of the enzyme was considered. The most suitable system for carrying o...

journal_title：Biotechnology and bioengineering

authors： Cremonesi P,Carrea G,Ferrara L,Antonini E

更新日期：1975-08-01 00:00:00

abstract：:Current approaches for purifying plasmids from bacterial production systems exploit the physiochemical properties of nucleic acids in non-specific capture systems. In this study, an affinity system for plasmid DNA (pDNA) purification has been developed utilizing the interaction between the lac operon (lacO) sequence c...

journal_title：Biotechnology and bioengineering

authors： Forde GM,Ghose S,Slater NK,Hine AV,Darby RA,Hitchcock AG

更新日期：2006-09-05 00:00:00

abstract：:It is well established that salt enhances the interaction between solutes (e.g., proteins, displacers) and the weak hydrophobic ligands in hydrophobic interaction chromatography (HIC) and that various salts (e.g., kosmotropes, chaotropes, and neutral) have different effects on protein retention. In this article, the s...

journal_title：Biotechnology and bioengineering

authors： Xia F,Nagrath D,Garde S,Cramer SM

更新日期：2004-08-05 00:00:00