Abstract：
:The efficiency of a versatile in vivo cascade involving a promiscuous alcohol dehydrogenase, obtained from a biodiversity search, and a Baeyer-Villiger monooxygenase was enhanced by the independent control of the production level of each enzyme to produce ε-caprolactone and 3,4-dihydrocoumarin. This goal was achieved by adjusting the copy number per cell of Escherichia coli plasmids. We started from the observation that this number generally correlates with the amount of produced enzyme and demonstrated that an in vivo multi-enzymatic system can be improved by the judicious choice of plasmid, the lower activity of the enzyme that drives the limiting step being counter-balanced by a higher concentration. Using a preconception-free approach to the choice of the plasmid type, we observed positive and negative synergetic effects, sometimes unexpected and depending on the enzyme and plasmid combinations. Experimental optimization of the culture conditions allowed us to obtain the complete conversion of cyclohexanol (16 mM) and 1-indanol (7.5 mM) at a 0.5-L scale. The yield for the conversion of cyclohexanol was 80% (0.7 g ε-caprolactone, for the productivity of 244 mg·L -1 ·h -1 ) and that for 1-indanol 60% (0.3 g 3,4-dihydrocoumarin, for the productivity of 140 mg·L -1 ·h -1 ).
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Ménil S,Petit JL,Courvoisier-Dezord E,Debard A,Pellouin V,Reignier T,Sergent M,Deyris V,Duquesne K,de Berardinis V,Alphand Vdoi
10.1002/bit.27133subject
Has Abstractpub_date
2019-11-01 00:00:00pages
2852-2863issue
11eissn
0006-3592issn
1097-0290journal_volume
116pub_type
杂志文章abstract：:Mixed-culture fermentation is a key central process to enable next generation biofuels and biocommodity production due to economic and process advantages over application of pure cultures. However, a key limitation to the application of mixed-culture fermentation is predicting culture product response, related to meta...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章,评审
doi：10.1002/bit.25321
更新日期：2014-11-01 00:00:00
abstract：:Anaerobic digestion of biological organic particulates to methane has been described by a structured mathematical model based on multiple-reaction stoichiometry, conventional material balances, and liquid phase equilibrium chemistry. A general stoichiometric treatment for any set of multiple biological reactions is de...
journal_title：Biotechnology and bioengineering
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更新日期：1985-05-01 00:00:00
abstract：:Temperature shifting was investigated as a means of improving cloned-gene product yields form a recombinant Escherichia coli containing the temperature-sensitive plasmid, pOU 140. In a series of shaker flask fermentations recombinant cells were thermally induced for different time periods. The growth, stability, and p...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260290416
更新日期：1987-03-01 00:00:00
abstract：:To alleviate plasmid instability and to prolong the production phase of subtilisin, integrable plasmid and spore mutants are used. Compared with batch-type shake flask cultures, spore mutants' ability to produce subtilisin can be well pronounced in fed-batch and continuous cultures. Hence, the two culture methods make...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260470610
更新日期：1995-09-20 00:00:00
abstract：:Traditional biological treatment models are "deduced" from formal chemical kinetics or dynamics of pure microorganism cultures growth. The best formal models give reasonable approximations of the biological treatment model with an ecosystem adaptation (ESA model). The model presented here explains some features of the...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260250610
更新日期：1983-06-01 00:00:00
abstract：:Chinese hamster ovary (CHO) cells in culture are known to consume large amounts of nutrients and divert most of them toward byproducts, some of which, including lactate and ammonia, are known to be toxic in nature. Glucose limitation strategies can successfully control lactate accumulation in fed-batch cultures yieldi...
journal_title：Biotechnology and bioengineering
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.26598
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260440213
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
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journal_title：Biotechnology and bioengineering
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pub_type： 杂志文章
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更新日期：1999-10-05 00:00:00
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章,评审
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journal_title：Biotechnology and bioengineering
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更新日期：1997-09-05 00:00:00
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260191204
更新日期：1977-12-01 00:00:00
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章,评审
doi：10.1002/bit.20096
更新日期：2004-10-05 00:00:00
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journal_title：Biotechnology and bioengineering
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doi：10.1002/bit.260310406
更新日期：1988-03-01 00:00:00
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/(sici)1097-0290(19980405)58:1<47::aid-bit5
更新日期：1998-04-05 00:00:00
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.22757
更新日期：2010-09-01 00:00:00
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journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
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更新日期：2017-11-01 00:00:00
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journal_title：Biotechnology and bioengineering
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journal_title：Biotechnology and bioengineering
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journal_title：Biotechnology and bioengineering
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