Development of a multienzyme reactor for dopamine synthesis: I. enzymology and kinetics.

abstract：:A model that portrays substrate profiles in a steady-state multispecies granular biofilm is developed and coupled with a biofilm detachment model. The model accounts for glucose, propionate, hydrogen, and acetate transformations performed by three bacterial trophic groups: acidogens, syntrophic bacterial consortia, an...

journal_title：Biotechnology and bioengineering

authors： Tartakovsky B,Guiot SR

更新日期：1997-04-20 00:00:00

abstract：:We consider the application of high-throughput experimentation (HTE), including combinatorial methods, to catalyst discovery and early-phase optimization. While combinatorial- and parallel-testing methods have an already substantial history in catalysis, recent work by several groups promises significant efficiency ga...

journal_title：Biotechnology and bioengineering

authors： Newsam JM,Schüth F

更新日期：1998-01-01 00:00:00

abstract：:Phaeodactylum tricornutum is a widely studied diatom and has been proposed as a source of oil and polyunsaturated fatty acids (PUFA), particularly eicosapentaenoic acid (EPA). Recent studies indicate that lipid accumulation occurs under nutritional stress. Aim of this research was to determine how changes in nitrogen ...

journal_title：Biotechnology and bioengineering

authors： Rodolfi L,Biondi N,Guccione A,Bassi N,D'Ottavio M,Arganaraz G,Tredici MR

更新日期：2017-10-01 00:00:00

abstract：:Mixed culture of microorganisms immobilized onto Celite diatomaceous earth particles were used to degrade 3,4-dichloroaniline (34DCA) in a three-phase draft tube fluidized bed bioreactor. Biodegradation was confirmed as the dominant removal mechanism by measurements of the concomitant chloride ion evolution. Degradati...

journal_title：Biotechnology and bioengineering

authors： Livingston AG

更新日期：1991-07-01 00:00:00

abstract：:A submerged anaerobic dynamic membrane bioreactor (AnDMBR) was operated for treatment of concentrated wastewater. The dynamic membrane (DM) or cake layer was characterized on its physicochemical and biological composition and the role of the DM layer in treatment and filtration performances was assessed. The results s...

journal_title：Biotechnology and bioengineering

authors： Ersahin ME,Tao Y,Ozgun H,Spanjers H,van Lier JB

更新日期：2016-04-01 00:00:00

abstract：:An immobilized enzyme reactor has been developed for the degradation of bilirubin as a potential treatment for neonatal jaundice. It utilizes the enzyme bilirubin oxidase from Myrothecium verrucaria, which in the presence of molecular oxygen converts bilirubin to biliverdin and other products that are much less toxic ...

journal_title：Biotechnology and bioengineering

authors： Sung C,Lavin A,Klibanov AM,Langer R

更新日期：1986-10-01 00:00:00

abstract：:Current techniques for characterizing biofilm physiology lack the signal filtering capability required for quantifying signals associated with real time biologically active transport. Though a great deal was learned from previous investigations, no results have been reported on the characterization of in vivo, real ti...

journal_title：Biotechnology and bioengineering

authors： McLamore ES,Porterfield DM,Banks MK

更新日期：2009-02-15 00:00:00

abstract：:A simple methodology has been successfully employed to explain the solvent dependence of the substrate specificity of enzymes in organic media. This methodology, which does not require the knowledge of the enzyme structure and is thus applicable to lyophilized and other noncrystalline enzyme preparations, predicts tha...

journal_title：Biotechnology and bioengineering

authors： Wescott CR,Klibanov AM

更新日期：1997-11-05 00:00:00

abstract：:Cell culture longevity in fed-batch culture of hybridomas is often limited by elevated medium osmolality caused by repeated nutrient feeding. Shotwise feeding of 10x Dulbecco's modified Eagle's medium (DMEM) concentrates elevated the osmolality of medium up to 540 mOsm/kg at the end of fed-batch culture of S3H5/gamma2...

journal_title：Biotechnology and bioengineering

authors： Ryu JS,Lee GM

更新日期：1999-01-05 00:00:00

abstract：:The downstream processing of virus particles for vaccination or gene therapy is becoming a critical bottleneck as upstream titers keep improving. Moreover, the growing pressure to develop cost-efficient processes has brought forward new downstream trains. This review aims at analyzing the state-of-the-art in viral dow...

journal_title：Biotechnology and bioengineering

authors： Nestola P,Peixoto C,Silva RR,Alves PM,Mota JP,Carrondo MJ

更新日期：2015-05-01 00:00:00

abstract：:Product inhibition of biological production systems is a widely observed phenomenon with prominent implications for the design and ultimately the success of biotechnological processes. In order to investigate whether such effects could limit the maximal concentrations in the production of the beta-lactamase inhibitor ...

journal_title：Biotechnology and bioengineering

authors： Brethauer S,Held M,Panke S

更新日期：2008-06-15 00:00:00

abstract：:Many biochemical processes consist of a sequence of operations for which optimal operating conditions (setpoints) have to be determined. If such optimization is performed for each operation separately with respect to objectives defined for each operation individually, overall process performance is likely to be subopt...

journal_title：Biotechnology and bioengineering

authors： Groep ME,Gregory ME,Kershenbaum LS,Bogle ID

更新日期：2000-02-05 00:00:00

abstract：:Oxygenases catalyze, among other interesting reactions, highly selective hydrocarbon oxyfunctionalizations, which are important in industrial organic synthesis but difficult to achieve by chemical means. Many enzymatic oxygenations have been described, but few of these have been scaled up to industrial scales, due to ...

journal_title：Biotechnology and bioengineering

authors： Bühler B,Bollhalder I,Hauer B,Witholt B,Schmid A

更新日期：2003-06-30 00:00:00

abstract：:Growth dynamics of Pseudomonas aeruginosa, Burkholderia cepacia, and Staphylococcus aureus in a batch and chemostat, were investigated as a laboratory model system for persistent infections in cystic fibrosis. Most species-specific enumeration methods for mixed cultures are laborious or only qualitative, and therefore...

journal_title：Biotechnology and bioengineering

authors： Schmidt JK,König B,Reichl U

更新日期：2007-03-01 00:00:00

abstract：:The kinetic behavior of soluble and insoluble forms of dextransucrase from Leuconostoc mesenteroides NRRL B-1299 was investigated with sucrose as substrate and maltose as acceptor. To study the parameters involved, a kinetic model was applied that was previously developed for L. mesenteroides NRRL B-512F dextransucras...

journal_title：Biotechnology and bioengineering

authors： Dols M,Remaud-Simeon M,Willemot RM,Demuth B,Jordening HJ,Buchholz K,Monsan P

更新日期：1999-05-05 00:00:00

abstract：:Small interfering RNAs (siRNAs) are invaluable research tools for studying gene functions in mammalian cells. siRNAs are mainly produced by chemical synthesis or by enzymatic digestion of double-stranded RNA (dsRNA) produced in vitro. Recently, bacterial cells, engineered with ectopic plant viral siRNA binding protein...

journal_title：Biotechnology and bioengineering

authors： Kaur G,Cheung HC,Xu W,Wong JV,Chan FF,Li Y,McReynolds L,Huang L

更新日期：2018-09-01 00:00:00

abstract：:Nanoparticles (NPs) are able to deliver a variety of substances into eukaryotic cells. However, their usage is often hampered by a lack of specificity, leading to the undesired uptake of NPs by virtually all cell types. In contrast to this, yeast is known to be specifically taken up into immune cells after entering th...

journal_title：Biotechnology and bioengineering

authors： Kiefer R,Jurisic M,Dahlem C,Koch M,Schmitt MJ,Kiemer AK,Schneider M,Breinig F

更新日期：2020-03-01 00:00:00

abstract：:The lignin peroxidases (LIP) and manganese peroxidases (MNP) of Phanerochaete chrysosporium catalyze a wide range of lignin depolymerization reactions with lignin models and synthetic lignins in solution. However, their ability to degrade insoluble natural lignin in aqueous media has not been demonstrated. Insoluble i...

journal_title：Biotechnology and bioengineering

authors： Thompson DN,Hames BR,Reddy CA,Grethlein HE

更新日期：1998-03-20 00:00:00

abstract：:This study demonstrates that masking the Feb regions of a monoclonal antibody (Mab) with synthetic antigens prior to covalent immobilization efficiency. Water-soluble adducts of poly(2-methyloxazoline) polymers and a synthetic-peptide epitope for the Mab were constructed. These synthetic antigens are referred to as Fa...

journal_title：Biotechnology and bioengineering

authors： Velander WH,Subramanian A,Madurawe RD,Orthner CL

更新日期：1992-04-25 00:00:00

abstract：:This article provides the analysis on biomass yields and energetic yields of the oleaginous yeasts. The biomass yields of the oleaginous yeasts are consistently lower than nonoleaginous microorganisms, whereas their energetic yields are higher. Data inconsistencies of literature are explained by the variation of energ...

journal_title：Biotechnology and bioengineering

authors： Pan JG,Rhee JS

更新日期：1986-01-01 00:00:00

abstract：:Adsorption of cellulases onto lignin is considered a major factor in retarding enzymatic cellulose degradation of lignocellulosic biomass. However, the adsorption mechanisms and kinetics are not well understood for individual types of cellulases. This study examines the binding affinity, kinetics of adsorption, and co...

journal_title：Biotechnology and bioengineering

authors： Djajadi DT,Pihlajaniemi V,Rahikainen J,Kruus K,Meyer AS

更新日期：2018-12-01 00:00:00

abstract：:It is known that the rolA, rolB, and rolC genes of Agrobacterium rhizogenes T-DNA affect processes of plant development and activate the synthesis of secondary metabolites in transformed plant cells. Although a synergistic activity of the rol genes on root formation is well-documented, little is known about their indi...

journal_title：Biotechnology and bioengineering

authors： Shkryl YN,Veremeichik GN,Bulgakov VP,Tchernoded GK,Mischenko NP,Fedoreyev SA,Zhuravlev YN

更新日期：2008-05-01 00:00:00

abstract：:The dissolved oxygen (DO) is an important variable in aerobic fermentations and affects the cell growth and product formation. Dissolved oxygen control is difficult in batch fermentations because of the time-varying conditions, time delays, and the probe dynamics. Modeling of the various patterns of biological activit...

journal_title：Biotechnology and bioengineering

authors： Sargantanis IG,Karim MN

更新日期：1998-10-05 00:00:00

abstract：:The evolution of microbial populations involved in simulated-heap leaching of a polymetallic black schist sulfide ore (from the recently-commissioned Talvivaara mine, Finland) was monitored in aerated packed bed column reactors over a period of 40 weeks. The influence of ore particle size (2-6.5 mm and 6.5-12 mm) on c...

journal_title：Biotechnology and bioengineering

authors： Wakeman K,Auvinen H,Johnson DB

更新日期：2008-11-01 00:00:00

abstract：:In this study, a non-sterile (open) continuous fermentation (OCF) process with no-carbon loss was developed to improve lactic acid (LA) productivity and operational stability from the co-utilization of lignocellulose-derived sugars by thermophilic Enterococcus faecium QU 50. The effects of different sugar mixtures on ...

journal_title：Biotechnology and bioengineering

authors： Abdel-Rahman MA,Tan J,Tashiro Y,Zendo T,Sakai K,Sonomoto K

更新日期：2020-06-01 00:00:00

abstract：:In this paper an extensive batch experiment of endogenous process behavior in an aerobic biodegradation process is presented. From these experimental data, comprising measurements of MLVSS (mixed liquor volatile suspended solids) and respiration rate, in a first step the states and unknown parameters in a four-compart...

journal_title：Biotechnology and bioengineering

authors： Keesman KJ,Spanjers H

更新日期：2000-05-20 00:00:00

abstract：:Trypsin and alpha-chymotrypsin were immobilized by gelentrapment in polyacrylamide cross-linked with N,N(1)-methylenebisacrylamide. The immobilized enzymes are catalytically efficient in suspensions of reverse micelles formed in isooctane by bis(2-ethylhexyl) sodium sulfosuccinate (AOT) and water. Both entrapped enzym...

journal_title：Biotechnology and bioengineering

authors： Fadnavis NW,Luisi PL

更新日期：1989-04-20 00:00:00

abstract：:Non-invasive methods for online monitoring of biotechnological processes without compromising the integrity of the reactor system are very important to generate continuous data. Even though calorimetry has been used in conventional biochemical analysis for decades, it has not yet been specifically applied for online d...

journal_title：Biotechnology and bioengineering

authors： Regestein L,Maskow T,Tack A,Knabben I,Wunderlich M,Lerchner J,Büchs J

更新日期：2013-05-01 00:00:00

abstract：:Acid phosphatase thermal deactivation follows a complex path: an initial decay toward an equilibrium distribution of at least two intermediate structures, mutually at the equilibrium, followed by a final breakdown toward a completely inactive enzyme configuration. The results obtained in the presence of sorbitol have ...

journal_title：Biotechnology and bioengineering

authors： Gianfreda L,Toscano G,Pirozzi D,Greco G Jr

更新日期：1991-12-05 00:00:00

abstract：:Helical-blade solids mixers have a large potential as bioreactors for solid-state fermentation (SSF). Fundamental knowledge of the flow and mixing behavior is required for robust operation of these mixers. In this study predictions of a discrete particle model were compared to experiments with colored wheat grain part...

journal_title：Biotechnology and bioengineering

authors： Schutyser MA,Briels WJ,Rinzema A,Boom RM

更新日期：2003-10-05 00:00:00