Oil and eicosapentaenoic acid production by the diatom Phaeodactylum tricornutum cultivated outdoors in Green Wall Panel (GWP®) reactors.

abstract：:Monolith reactors combine good mass transfer characteristics with low-pressure drop, the principle factors affecting the cost effectiveness of industrial processes. Recently, these specific features of the monolith reactors have drawn the attention toward the application of the monolith reactor in multiphase reaction ...

journal_title：Biotechnology and bioengineering

authors： Ebrahimi S,Kleerebezem R,Kreutzer MT,Kapteijn F,Moulijn JA,Heijnen JJ,van Loosdrecht MC

更新日期：2006-02-05 00:00:00

abstract：:A model for the prediction of protein partition coefficients in aqueous two-phase systems has been developed. This model accounts for both charge-independent and electrostatic effects. The determination of nonelectrostatic effects was based on the model of Eiteman and Gainer for uncharged solutes while the electrostat...

journal_title：Biotechnology and bioengineering

authors： Sebastião MJ,Martel P,Baptista A,Petersen SB,Cabral JM,Aires-Barros MR

更新日期：1997-11-05 00:00:00

abstract：:The production of recombinant proteins by mammalian cells demands a highly controlled environment for cell cultivation. Temperature stress represents a commonly encountered disturbance in both research and process environments. In this study, we examined the effects of heat shock on the expression of recombinant human...

journal_title：Biotechnology and bioengineering

authors： Tsao EI,Bohn MA,Omstead DR,Munster MJ,Numsuwan V

更新日期：1992-12-05 00:00:00

abstract：:The removal of N-compounds in the sequencing batch airlift reactor (SBAR) containing granular sludge was studied under conditions of decreased dissolved oxygen (DO). A simulation model was developed to describe and evaluate the effects of several process conditions in the SBAR on N-removal performance. The model descr...

journal_title：Biotechnology and bioengineering

authors： Beun JJ,Heijnen JJ,van Loosdrecht MC

更新日期：2001-10-05 00:00:00

abstract：:Kluyveromyces marxianus UCD (FST) 55-82 cells were immobilized in Na alginate beads and used in a packed-bed bioreactor system for the continuous production of ethanol from the extract of Jerusalem artichoke tubers. Volumetric ethanol productivities of 104 and 80 g ethanol/ L/h were obtained at 80 and 92% sugar utiliz...

journal_title：Biotechnology and bioengineering

authors： Margaritis A,Bajpai P

更新日期：1982-07-01 00:00:00

abstract：:Studies have been conducted in a gas circulation type fermentor in order to characterize the ethanol fermentation of uncooked cassava starch with Rhizopus koji. Results showed that ethanol concentration reached 13-14% (v/v) in 4-day broth, and the maximum productivity of ethanol was 2.3 g ethanol/L broth h. This produ...

journal_title：Biotechnology and bioengineering

authors： Fujio Y,Ogata M,Ueda S

更新日期：1985-08-01 00:00:00

abstract：:Production of cellulase using solid culture systems of Trichoderma reesei QM9414 and Sporotrichum cellulophilum on wheat bran was studied. By using moisture-controlled solid culture equipment, the effect of water content of wheat bran on cell growth and cellulase production was investigated. Cellular biomass grown on ...

journal_title：Biotechnology and bioengineering

authors： Kim JH,Hosobuchi M,Kishimoto M,Seki T,Yoshida T,Taguchi H,Ryu DD

更新日期：1985-10-01 00:00:00

abstract：:Cross-linked polyurethane (PU) was prepared for entrapping thermolysin. Using the immobilized thermolysin (IT), Z-L-aspartic acid (ZA) was reacted with -Lphenylalanine methyl ester (L-PM) in water-saturated ethyl acetate to give only alpha-Z-L-aspartylL-phenylalanine methyl ester (alpha-ZAPM). Ninety-four percent conv...

journal_title：Biotechnology and bioengineering

authors： Yang CP,Su CS

更新日期：1988-08-20 00:00:00

abstract：:Insulin is an important biotherapeutic protein, and it is also a model protein used to study amyloid diseases, such as Alzheimer's and Parkinson's. The preparation of the protein can lead to small amounts of aggregate in the solution, which in turn may lead to irreproducible in vitro results. Using several pre-treatme...

journal_title：Biotechnology and bioengineering

authors： Heldt CL,Sorci M,Posada D,Hirsa A,Belfort G

更新日期：2011-01-01 00:00:00

abstract：:Trypsin and alpha-chymotrypsin were immobilized by gelentrapment in polyacrylamide cross-linked with N,N(1)-methylenebisacrylamide. The immobilized enzymes are catalytically efficient in suspensions of reverse micelles formed in isooctane by bis(2-ethylhexyl) sodium sulfosuccinate (AOT) and water. Both entrapped enzym...

journal_title：Biotechnology and bioengineering

authors： Fadnavis NW,Luisi PL

更新日期：1989-04-20 00:00:00

abstract：:The dissociation constant, K(d) , is an important parameter for characterizing protein-protein interaction affinities. SUMOylation is one of the important protein post-translational modifications and it involves a multi-step enzymatic cascade reaction, resulting in peptide activation and substrate conjugation. Multipl...

journal_title：Biotechnology and bioengineering

authors： Song Y,Rodgers VG,Schultz JS,Liao J

更新日期：2012-11-01 00:00:00

abstract：:Growth decoupling can be used to optimize the production of biochemicals and proteins in cell factories. Inhibition of excess biomass formation allows for carbon to be utilized efficiently for product formation instead of growth, resulting in increased product yields and titers. Here, we used CRISPR interference to in...

journal_title：Biotechnology and bioengineering

authors： Landberg J,Wright NR,Wulff T,Herrgård MJ,Nielsen AT

更新日期：2020-08-18 00:00:00

abstract：:Pseudomonads are serious candidates for siderophore production applied to toxic metal (TM) solubilization. The bioaugmentation of contaminated soils by these TM-solubilizing bacteria combined with phytoextraction is an emerging clean-up technology. Unfortunately, siderophore synthesis may be drastically reduced by sol...

journal_title：Biotechnology and bioengineering

authors： Braud A,Jézéquel K,Léger MA,Lebeau T

更新日期：2006-08-20 00:00:00

abstract：:A new ultrafiltration technique based on a multimembrane stack has been developed to fractionate solutes closer in size than conventionally possible. The technique is illustrated here by obtaining a pure protein product from a binary protein mixture. By employing membranes in series without any gaskets or spacers in-b...

journal_title：Biotechnology and bioengineering

authors： Feins M,Sirkar KK

更新日期：2004-06-20 00:00:00

abstract：:An option for fertility preservation for women facing a cancer diagnosis involves the cryopreservation of ovarian tissue for later re-transplantation or in vitro culture, with in vitro culture preferred to avoid reintroduction of the cancer. Small, immature follicles survive the freeze-thaw process, and can be matured...

journal_title：Biotechnology and bioengineering

authors： Xu M,Banc A,Woodruff TK,Shea LD

更新日期：2009-06-01 00:00:00

abstract：:We propose that transcript levels for some genes are affected by the bacterial cell division cycle and this may be an important factor to consider when designing synthetic bacterial genomes. To test this hypothesis, transcript levels of 58 genes in Escherichia coli B/r A were determined at five times during the cell d...

journal_title：Biotechnology and bioengineering

authors： Echtenkamp PL,Wilson DB,Shuler ML

更新日期：2009-02-15 00:00:00

abstract：:The simultaneous biodegradation of the nonionic surfactant Tween 20 (Tw20) and pentachlorophenol (PCP) by Sphingomonas chlorophenolica sp. Strain RA2 (RA2) was measured. As a sole substrate, Tw20 biodegradation was best described by the Contois kinetic model. During concurrent biodegradation of Tw20 and PCP, the biode...

journal_title：Biotechnology and bioengineering

authors： Bielefeldt AR,Cort T

更新日期：2005-03-20 00:00:00

abstract：:Bacillus licheniformis ATCC 9945A was grown on Medium E in batch fermentations in which the pH was maintained at 5.5., 6.5, 7.4, and 8.25. The effects of pH on cell growth, carbon source utilization, and gamma-polyglutamic acid (gamma-PGA) production, molecular weight, and polymer stereochemistry were determined. The ...

journal_title：Biotechnology and bioengineering

authors： Cromwick AM,Birrer GA,Gross RA

更新日期：1996-04-20 00:00:00

abstract：:Diffusion of phenol through a biofilm attached to activated carbon particles was investigated. The biofilm was grown on activated carbon particles in a draft-tube three-phase fluidized-bed bioreactor operating in a fed-batch mode. It was found that phenol did not adsorb on the biofilm and that the diffusion coefficien...

journal_title：Biotechnology and bioengineering

authors： Fan LS,Leyva-Ramos R,Wisecarver KD,Zehner BJ

更新日期：1990-02-05 00:00:00

abstract：:A stimulus-response tracer technique has been used to characterize packed beds of untreated, as well as acid prehydrolyzed, and enzymatically hydrolyzed aspen wood chips. Glucose was used as the tracer. Bulk liquid phase dispersion, interphase mass transfer, and intraparticle diffusion coefficients were determined for...

journal_title：Biotechnology and bioengineering

authors： Hradil G,Calo JM,Wunderlich TK Jr

更新日期：1993-02-05 00:00:00

abstract：:The effects of dilution rates on the performance of a two-stage fermentation system for a recombinant Escherichia coli culture were studied. Dilution rate determines the apparent or averaged specific growth rate of a heterogeneous population of cells in the recombinant culture. The specific growth rate affects the gen...

journal_title：Biotechnology and bioengineering

authors： Hortacsu A,Ryu DD

更新日期：1991-10-20 00:00:00

abstract：:Numerous biotechnological production processes are based on the submerse cultivation of filamentous fungi. Process design, however, is often hampered by the complex growth pattern of these organisms. In the morphologic development of coagulating filamentous fungi, like Aspergillus niger, conidial aggregation is the fi...

journal_title：Biotechnology and bioengineering

authors： Lin PJ,Grimm LH,Wulkow M,Hempel DC,Krull R

更新日期：2008-02-01 00:00:00

abstract：:A specially designed model reactor based on a 42-L laboratory fermentor was equipped with six stirrers (Rushton turbines) and five cylindrical disks. In this model reactor, the mixing time, Theta(90), turned out to be 13 times longer compared with the 42-L standard laboratory fermentor fitted with two Rushton turbines...

journal_title：Biotechnology and bioengineering

authors： Schilling BM,Pfefferle W,Bachmann B,Leuchtenberger W,Deckwer W

更新日期：1999-09-05 00:00:00

abstract：:The paper reviews the state-of-the-art in computational modeling of thrombus formation and growth and related phenomena including platelet margination, activation, adhesion, and embolization. Presently, there is a high degree of empiricism in the modeling of thrombus formation. Based on the experimentally observed phy...

journal_title：Biotechnology and bioengineering

authors： Hosseinzadegan H,Tafti DK

更新日期：2017-10-01 00:00:00

abstract：:Cellulase production from lignocellulosic materials was studied in solid-state cultivation by both static and mixed techniques under nonaseptic conditions. The effects of fermentation conditions, such as moisture content, pH, temperature, and aeration, on cellulase production by Trichoderma harzianum using a mixture o...

journal_title：Biotechnology and bioengineering

authors： Deschamps F,Giuliano C,Asther M,Huet MC,Roussos S

更新日期：1985-09-01 00:00:00

abstract：:Two metabolic engineering tools, namely gene inactivation and gene overexpression, were employed to examine the effects of two genetic modifications on the fermentation characteristics of Clostridium acetobutylicum. Inactivation of the butyrate kinase gene (buk) was examined using strain PJC4BK, while the combined eff...

journal_title：Biotechnology and bioengineering

authors： Harris LM,Desai RP,Welker NE,Papoutsakis ET

更新日期：2000-01-05 00:00:00

abstract：:An extractive acetonobutylic fermentation process is developed by integrating bioproduction, Ultrafiltration, and distillation, providing simultaneous retention of biomass, selective removal of inhibitors from the permeate, as well as separation and purification of acetone-butanol-ethanol solvents. Successive batch fe...

journal_title：Biotechnology and bioengineering

authors： Minier M,Grateloup R,Blanc-Ferras E,Goma G

更新日期：1990-04-15 00:00:00

abstract：:Cell-division-cycle, temperature-sensitive mutants of Saccharomyces cerevisiae were investigated as a means of altering the morphological characteristics and subsequent physical properties of single-cell protein (SCP). Strain 4471, harboring mutation cdc 4, formed a visible complex mass at the nonpermissive temperatur...

journal_title：Biotechnology and bioengineering

authors： Miyasaka Y,Rha C,Sinskey AJ

更新日期：1980-10-01 00:00:00

abstract：:Use of cellulase for denim washing is a standard eco-friendly technique to achieve desirable appearance and softness for cotton fabrics and denims. But enzymatic washing of denim till date involved acid cellulase (Trichoderma reesei) and neutral cellulase (Humicola isolens) the use of which has a drawback of backstain...

journal_title：Biotechnology and bioengineering

authors： Anish R,Rahman MS,Rao M

更新日期：2007-01-01 00:00:00

abstract：:The adsorption of cellulase on cellulose and a lignacious residue was examined by using cellulase from Trichoderma reesei, hardwood pretreated by dilute sulfuric acid under high pressure, and a lignacious residue prepared by a complete enzymatic hydrolysis of the pretreated wood. A significant amount of cellulase was ...

journal_title：Biotechnology and bioengineering

authors： Ooshima H,Burns DS,Converse AO

更新日期：1990-08-20 00:00:00