Adaptive pole placement control algorithm for DO-control in beta-lactamase production.

abstract：:Spores, although often considered metabolically inert, catalyze a variety of reactions. The use of spores instead of mycelium for bioconversions has several advantages. In this paper, we describe the difference in susceptibility of mycelium and spores against toxic substrates and products. A higher resistance of spore...

journal_title：Biotechnology and bioengineering

authors： Wolken WA,Tramper J,van der Werf MJ

更新日期：2002-12-20 00:00:00

abstract：:Two metabolic engineering tools, namely gene inactivation and gene overexpression, were employed to examine the effects of two genetic modifications on the fermentation characteristics of Clostridium acetobutylicum. Inactivation of the butyrate kinase gene (buk) was examined using strain PJC4BK, while the combined eff...

journal_title：Biotechnology and bioengineering

authors： Harris LM,Desai RP,Welker NE,Papoutsakis ET

更新日期：2000-01-05 00:00:00

abstract：:We developed a method for the fast sorting and selection of mammalian cells expressing and secreting a protein at high levels. This procedure relies on cell capture using an automated microfluidic device handling antibody-coupled magnetic microparticles and on a timed release of the cells from the microparticles after...

journal_title：Biotechnology and bioengineering

authors： Droz X,Harraghy N,Lançon E,Le Fourn V,Calabrese D,Colombet T,Liechti P,Rida A,Girod PA,Mermod N

更新日期：2017-08-01 00:00:00

abstract：:Formaldehyde is a prevalent environmental toxin and a key intermediate in single carbon metabolism. The ability to monitor formaldehyde concentration is, therefore, of interest for both environmental monitoring and for metabolic engineering of native and synthetic methylotrophs, but current methods suffer from low sen...

journal_title：Biotechnology and bioengineering

authors： Woolston BM,Roth T,Kohale I,Liu DR,Stephanopoulos G

更新日期：2018-01-01 00:00:00

abstract：:A spinning cylindrical filter is often used to retain mammalian cells in a continuous perfusion bioreactor. This device, known as a spinfilter, has typically been with pore size smaller than the cell particles (single cells or aggregates) in order to achieve cell separation. For single cells in suspension, such an ope...

journal_title：Biotechnology and bioengineering

authors： Yabannavar VM,Singh V,Connelly NV

更新日期：1992-10-20 00:00:00

abstract：:Non-autologous somatic gene therapy is an alternate approach to delivering recombinant gene products through implantation of a "universal" donor cell line engineered to produce a therapeutic gene product. The cells are immunologically isolated by enclosure in immunoprotective microcapsules fabricated from alginate-pol...

journal_title：Biotechnology and bioengineering

authors： Awrey DE,Tse M,Hortelano G,Chang PL

更新日期：1996-11-20 00:00:00

abstract：:Both the forward and backward reactions of xylose isomerase (Sweetzyme Q) with xylose and glucose as substrates have been studied in terms of kinetics and thermodynamics. The relationship between the two reactions can thus be determined. Much attention has been given to the reaction with xylose as substrate. The optim...

journal_title：Biotechnology and bioengineering

authors： Olivier SP,du Toit PJ

更新日期：1986-05-01 00:00:00

abstract：:Miniaturized bioreactors for suspension cultures of animal cells, such as Chinese Hamster Ovary (CHO) cells, could improve bioprocess development through the ability to cheaply explore a wide range of bioprocess operating conditions. A miniaturized pressure-cycled bioreactor for animal cell cultures, described previou...

journal_title：Biotechnology and bioengineering

authors： Kim BJ,Zhao T,Young L,Zhou P,Shuler ML

更新日期：2012-01-01 00:00:00

abstract：:Microfiltration is an important unit operation in downstream processing. However, due to the influence of membrane fouling, prediction of the filtration performance for biological suspensions is difficult. This paper describes a modeling approach that allows a comprehensive description of filtration performance. On th...

journal_title：Biotechnology and bioengineering

authors： Meyer F,Gehmlich I,Guthke R,Górak A,Knorre WA

更新日期：1998-07-20 00:00:00

abstract：:All bacteria release organic compounds called soluble microbial products (SMP) as a part of their normal metabolism. In photobioreactor (PBR) settings, SMP produced by cyanobacteria represent a major pool of carbon and electrons available to heterotrophic bacteria. Thus, SMP in PBRs are a major driver for the growth o...

journal_title：Biotechnology and bioengineering

authors： Zevin AS,Nam T,Rittmann B,Krajmalnik-Brown R

更新日期：2015-09-01 00:00:00

abstract：:Copolymers of N-isopropylacrylamide and N-acryloyl amino acid spacers of varying chain length were synthesized. p-Aminobenzamidine (PABA) was chemically linked to the pendant carboxyl groups of these polymers to obtain thermoprecipitating affinity polymers. The inhibition constant (Ki) of these polymers for trypsin de...

journal_title：Biotechnology and bioengineering

authors： Vaidya AA,Lele BS,Kulkarni MG,Mashelkar RA

更新日期：1999-08-20 00:00:00

abstract：:Cellulase was modified with amphilic copolymers made of alpha-allyl-omega-methoxy polyoxyalkylene (POA) and maleic acid anhydride (MAA) to improve the cellulose hydrolytic reactivity and cellulase separation. Amino groups of the cellulase molecule are covalently coupled with the MAA functional groups of the copolymer....

journal_title：Biotechnology and bioengineering

authors： Park JW,Kajiuchi T

更新日期：1995-02-20 00:00:00

abstract：:Rate and yield expressions relating to biomass and xanthan formation and to nitrogen, glucose, and oxygen consumption were established for Xanthomonas campestris batch fermentations in a bubble column. Microbial growth was described by the logistic rate equation, characterized by a maximum specific growth rate mu(M) =...

journal_title：Biotechnology and bioengineering

authors： Pons A,Dussap CG,Gros JB

更新日期：1989-01-20 00:00:00

abstract：:A limited life cycle assessment (LCA) was performed on a combined biological and chemical process for the production of adipic acid, which was compared to the traditional petrochemical process. The LCA comprises the biological conversion of the aromatic feedstocks benzoic acid, impure aromatics, toluene, or phenol fro...

journal_title：Biotechnology and bioengineering

authors： van Duuren JB,Brehmer B,Mars AE,Eggink G,Dos Santos VA,Sanders JP

更新日期：2011-06-01 00:00:00

abstract：:Streamline Direct CST I is a new type of ion exchanger with multi-modal functional groups, specially designed for an expanded bed adsorption (EBA) process, which can capture directly the proteins from the high ionic strength feedstocks with a high binding capacity. In this study, an experimental study is carried out f...

journal_title：Biotechnology and bioengineering

authors： Li P,Xiu G,Mata VG,Grande CA,Rodrigues AE

更新日期：2006-08-20 00:00:00

abstract：:In this paper an extensive batch experiment of endogenous process behavior in an aerobic biodegradation process is presented. From these experimental data, comprising measurements of MLVSS (mixed liquor volatile suspended solids) and respiration rate, in a first step the states and unknown parameters in a four-compart...

journal_title：Biotechnology and bioengineering

authors： Keesman KJ,Spanjers H

更新日期：2000-05-20 00:00:00

abstract：:Substrate transfer rates from organic to aqueous phases were measured in the presence and absence of biocatalyst in the reaction medium, using modified Lewis cells. These measurements, in combination with intrinsic aqueous phase biocatalytic reaction kinetics, were used to confirm that benzyl acetate hydrolysis by pig...

journal_title：Biotechnology and bioengineering

authors： Woodley JM,Brazier AJ,Lilly MD

更新日期：1991-01-20 00:00:00

abstract：:The use of microalgae for biofuel production will be beneficial to society if we can produce biofuels at large scales with minimal mechanical energy input in the production process. Understanding micro-algal physiological responses under variable environmental conditions in bioreactors is essential for the optimizatio...

journal_title：Biotechnology and bioengineering

authors： Hansen TJ,Hondzo M,Mashek MT,Mashek DG,Lefebvre PA

更新日期：2013-01-01 00:00:00

abstract：:The adsorption of cellulase on cellulose and a lignacious residue was examined by using cellulase from Trichoderma reesei, hardwood pretreated by dilute sulfuric acid under high pressure, and a lignacious residue prepared by a complete enzymatic hydrolysis of the pretreated wood. A significant amount of cellulase was ...

journal_title：Biotechnology and bioengineering

authors： Ooshima H,Burns DS,Converse AO

更新日期：1990-08-20 00:00:00

abstract：:The impact of process environment changes on process performance is one of the most crucial process safety issues when cultivating mammalian cells in a bioreactor. In contrast, directed shifting of process parameters can also be used as an optimization tool providing higher cell and product yields. Compared to other s...

journal_title：Biotechnology and bioengineering

authors： Trummer E,Fauland K,Seidinger S,Schriebl K,Lattenmayer C,Kunert R,Vorauer-Uhl K,Weik R,Borth N,Katinger H,Müller D

更新日期：2006-08-20 00:00:00

abstract：:The end-product profile of the glucose fermentation by Propionibacterium freudenreichii ET-3 changed on an electrochemical treatment, in which the culture vessel was filled with a carbon felt anode. Acetate and propionate were produced as final end products in a molar ratio of 2:3 without any electrochemical treatment...

journal_title：Biotechnology and bioengineering

authors： Wang YF,Masuda M,Tsujimura S,Kano K

更新日期：2008-10-15 00:00:00

abstract：:Co-Immobilization of dextransucrase (DS) and dextranase (DN) into calcium alginate includes the co-entrapment of soluble DS and adsorbed DN. DS converts sucrose into dextran, which is the substrate for DN, so that isomalto-oligosaccharides (IMOs) are follow-up products of dextran hydrolysis. The boundary conditions fo...

journal_title：Biotechnology and bioengineering

authors： Erhardt FA,Kügler J,Chakravarthula RR,Jördening HJ

更新日期：2008-07-01 00:00:00

abstract：:Bioartificial liver (BAL) devices have been developed to treat patients undergoing acute liver failure. One of the most important parameters to consider in designing these devices is the oxygen consumption rate of the seeded hepatocytes which are known to have oxygen consumption rates 10 times higher than most other c...

journal_title：Biotechnology and bioengineering

authors： Cho CH,Park J,Nagrath D,Tilles AW,Berthiaume F,Toner M,Yarmush ML

更新日期：2007-05-01 00:00:00

abstract：:Clostridium tyrobutyricum ATCC 25755 is known as a natural hyper-butyrate producer with great potentials as an excellent platform to be engineered for valuable biochemical production from renewable resources. However, limited transformation efficiency and the lack of genetic manipulation tools have hampered the broade...

journal_title：Biotechnology and bioengineering

authors： Zhang J,Hong W,Guo L,Wang Y,Wang Y

更新日期：2020-09-01 00:00:00

abstract：:A model is developed to predict batch and continuous culture behavior of fermentations on two different carbon and energy sources. The basic assumption of the model is that the permease for the favored substrate is constitutive, whereas the premease for the second substrate is subject to induction and catabolite repre...

journal_title：Biotechnology and bioengineering

authors： Van Dedem G,Moo-Young M

更新日期：1975-09-01 00:00:00

abstract：:Myristic acid esterification has been performed by an immobilized lipase from Mucor Miehei both in n-hexane and in supercritical carbon dioxide (SCCO(2)). The enzyme is stable in SCCO(2) at 15 MPa and 323 K. The reaction rate is influenced by the concentration of water and by the reaction medium composition. A reactio...

journal_title：Biotechnology and bioengineering

authors： Dumont T,Barth D,Corbier C,Branlant G,Perrut M

更新日期：1992-06-20 00:00:00

abstract：:A major challenge in downstream purification of monoclonal antibodies (mAb) is the removal of host cell proteins (HCPs). Previous studies have shown that cell culture conditions significantly impact the HCP content at harvest. However, it is currently unclear how process conditions affect physiological changes in the ...

journal_title：Biotechnology and bioengineering

authors： Goey CH,Tsang JMH,Bell D,Kontoravdi C

更新日期：2017-12-01 00:00:00

abstract：:In this study we report the isolation of four denitrifying bacteria from a batch reactor, where the progress of hydrogenotrophic denitrification was examined. Only three of the strains had the ability to use hydrogen as electron donor. In the present work, kinetic batch experiments were carried out in order to study t...

journal_title：Biotechnology and bioengineering

authors： Vasiliadou IA,Siozios S,Papadas IT,Bourtzis K,Pavlou S,Vayenas DV

更新日期：2006-10-20 00:00:00

abstract：:Three-dimensional cell culture models, such as spheroids, can be used in the process of the development of new anticancer agents because they are able to closely mimic the main features of human solid tumors, namely their structural organization, cellular layered assembling, hypoxia, and nutrient gradients. These prop...

journal_title：Biotechnology and bioengineering

authors： Nunes AS,Barros AS,Costa EC,Moreira AF,Correia IJ

更新日期：2019-01-01 00:00:00

abstract：:A software sensor was developed to determine the volumetric biomass activity of animal cell cultivations on-line. It was based on the on-line estimation of the ATP-production rate from the oxygen uptake and the lactic-acid production rate. The sensor was verified for a batch culture of Vero cells, and a batch and a co...

journal_title：Biotechnology and bioengineering

authors： Dorresteijn RC,Numan KH,de Gooijer CD,Tramper J,Beuvery EC

更新日期：1996-07-20 00:00:00