A limited LCA of bio-adipic acid: manufacturing the nylon-6,6 precursor adipic acid using the benzoic acid degradation pathway from different feedstocks.


:A limited life cycle assessment (LCA) was performed on a combined biological and chemical process for the production of adipic acid, which was compared to the traditional petrochemical process. The LCA comprises the biological conversion of the aromatic feedstocks benzoic acid, impure aromatics, toluene, or phenol from lignin to cis, cis-muconic acid, which is subsequently converted to adipic acid through hydrogenation. Apart from the impact of usage of petrochemical and biomass-based feedstocks, the environmental impact of the final concentration of cis, cis-muconic acid in the fermentation broth was studied using 1.85% and 4.26% cis, cis-muconic acid. The LCA focused on the cumulative energy demand (CED), cumulative exergy demand (CExD), and the CO(2) equivalent (CO(2) eq) emission, with CO(2) and N(2) O measured separately. The highest calculated reduction potential of CED and CExD were achieved using phenol, which reduced the CED by 29% and 57% with 1.85% and 4.26% cis, cis-muconic acid, respectively. A decrease in the CO(2) eq emission was especially achieved when the N(2) O emission in the combined biological and chemical process was restricted. At 4.26% cis, cis-muconic acid, the different carbon backbone feedstocks contributed to an optimized reduction of CO(2) eq emissions ranging from 14.0 to 17.4 ton CO(2) eq/ton adipic acid. The bulk of the bioprocessing energy intensity is attributed to the hydrogenation reactor, which has a high environmental impact and a direct relationship with the product concentration in the broth.


Biotechnol Bioeng


van Duuren JB,Brehmer B,Mars AE,Eggink G,Dos Santos VA,Sanders JP




Has Abstract


2011-06-01 00:00:00












  • Design and construction of improved new vectors for Zymomonas mobilis recombinants.

    abstract::Zymomonas mobilis is a very important gram-negative bacterium having a potential application to simultaneous co-production of biofuel and other high value-added products through biorefinery process technology development. Up to now, pLOI193 has been used as the plasmid of choice for Z. mobilis strains. However, its ap...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Dong HW,Bao J,Ryu DD,Zhong JJ

    更新日期:2011-07-01 00:00:00

  • Cultivation of hybridoma cells in an inclined bioreactor.

    abstract::Murine hybridoma cells were grown in a bubble column that was inclined up to 45 degrees from vertical. Inclining the column by a few degrees separated the rising bubbles against the upper surface, leaving the bulk of the liquid bubble free. The liquid was circulated well by the rising bubbles, but collection of cells ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Lu GZ,Gray MR,Thompson BG,Suresh MR

    更新日期:1995-01-20 00:00:00

  • Metabolic modeling for predicting VFA production from protein-rich substrates by mixed-culture fermentation.

    abstract::Proteinaceous organic wastes are suitable substrates to produce high added-value products in anaerobic mixed-culture fermentations. In these processes, the stoichiometry of the biotransformation depends highly on operational conditions such as pH or feeding characteristics and there are still no tools that allow the p...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Regueira A,Lema JM,Carballa M,Mauricio-Iglesias M

    更新日期:2020-01-01 00:00:00

  • Chemical inhibition of nitrification in activated sludge.

    abstract::Conventional aerobic nitrification was adversely affected by single pulse inputs of six different classes of industrially relevant chemical toxins: an electrophilic solvent (1-chloro-2,4-dinitrobenzene, CDNB), a heavy metal (cadmium), a hydrophobic chemical (1-octanol), an uncoupling agent (2,4-dinitrophenol, DNP), al...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Kelly RT 2nd,Henriques ID,Love NG

    更新日期:2004-03-20 00:00:00

  • Endogenous model state and parameter estimation from an extensive batch experiment.

    abstract::In this paper an extensive batch experiment of endogenous process behavior in an aerobic biodegradation process is presented. From these experimental data, comprising measurements of MLVSS (mixed liquor volatile suspended solids) and respiration rate, in a first step the states and unknown parameters in a four-compart...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Keesman KJ,Spanjers H

    更新日期:2000-05-20 00:00:00

  • Effects of cellobiose and glucose on cellulose hydrolysis by both growing and resting cells of Bacteroides cellulosolvens.

    abstract::The cellulase system of Bacteroides cellulosolvens was subjected to both catabolite repression and feedback inhibition by cellobiose. Cellulose-solubilizing activity was 50% inhibited at a cellobiose concentration of 2.6 g/L and completely inhibited by 12 g/L. Glucose at 12 g/L (the highest concentration tested) had n...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Murray WD

    更新日期:1987-06-01 00:00:00

  • Active-site titration of serine proteases in organic solvents.

    abstract::Calculation of kinetic constants of an enzymatic reaction in organic solvents requires knowledge of the functional active-site concentration in organic solvents, and this can be significantly different than that in water. An experimental method for active-site titration of serine proteases in organic media has been de...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Wangikar PP,Carmichael D,Clark DS,Dordick JS

    更新日期:1996-05-05 00:00:00

  • Evaluation of heart tissue viability under redox-magnetohydrodynamics conditions: toward fine-tuning flow in biological microfluidics applications.

    abstract::A microfluidic system containing a chamber for heart tissue biopsies, perfused with Krebs-Henseleit buffer containing glucose and antibiotic (KHGB) using peristaltic pumps and continuously stimulated, was used to evaluate tissue viability under redox-magnetohydrodynamics (redox-MHD) conditions. Redox-MHD possesses uni...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Cheah LT,Fritsch I,Haswell SJ,Greenman J

    更新日期:2012-07-01 00:00:00

  • Individual and combined effects of the rolA, B, and C genes on anthraquinone production in Rubia cordifolia transformed calli.

    abstract::It is known that the rolA, rolB, and rolC genes of Agrobacterium rhizogenes T-DNA affect processes of plant development and activate the synthesis of secondary metabolites in transformed plant cells. Although a synergistic activity of the rol genes on root formation is well-documented, little is known about their indi...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Shkryl YN,Veremeichik GN,Bulgakov VP,Tchernoded GK,Mischenko NP,Fedoreyev SA,Zhuravlev YN

    更新日期:2008-05-01 00:00:00

  • Metabolic engineering of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) composition in recombinant Salmonella enterica serovar typhimurium.

    abstract::A recombinant strain of Salmonella enterica serovar Typhimurium (mutant in propionate-activation activity) was metabolically engineered to control the composition of poly(3-hydroxybutyrate-co-3-hydroxy- valerate) (PHBV), a polyhydroxyalkanoate copolymer with commercially desirable properties. A gene (prpE) encoding pr...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Aldor I,Keasling JD

    更新日期:2001-09-01 00:00:00

  • Combinatorial synthetic pathway fine-tuning and comparative transcriptomics for metabolic engineering of Raoultella ornithinolytica BF60 to efficiently synthesize 2,5-furandicarboxylic acid.

    abstract::The compound 5-hydroxymethylfurfural (HMF) has attracted much attention due to its versatility as an important bio-based platform chemical. Here, we engineered Raoultella ornithinolytica BF60 as a whole-cell biocatalyst for a highly efficient synthesis of 2,5-furandicarboxylic acid (FDCA) from HMF. Specifically, vario...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Yuan H,Liu Y,Li J,Shin HD,Du G,Shi Z,Chen J,Liu L

    更新日期:2018-09-01 00:00:00

  • Design of cellular porous biomaterials for wall shear stress criterion.

    abstract::The microfluidic environment provided by implanted prostheses has a decisive influence on the viability, proliferation and differentiation of cells. In bone tissue engineering, for instance, experiments have confirmed that a certain level of wall shear stress (WSS) is more advantageous to osteoblastic differentiation....

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Chen Y,Zhou S,Cadman J,Li Q

    更新日期:2010-11-01 00:00:00

  • DNA-crosslinker cisplatin eradicates bacterial persister cells.

    abstract::For all bacteria, nearly every antimicrobial fails since a subpopulation of the bacteria enter a dormant state known as persistence, in which the antimicrobials are rendered ineffective due to the lack of metabolism. This tolerance to antibiotics makes microbial infections the leading cause of death worldwide and make...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Chowdhury N,Wood TL,Martínez-Vázquez M,García-Contreras R,Wood TK

    更新日期:2016-09-01 00:00:00

  • Progression of continuous downstream processing of monoclonal antibodies: Current trends and challenges.

    abstract::Rapid advances in intensifying upstream processes for biologics production have left downstream processing as a bottleneck in the manufacturing scheme. Biomanufacturers are pursuing continuous downstream process development to increase efficiency and flexibility, reduce footprint and cost of goods, and improve product...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章,评审


    authors: Somasundaram B,Pleitt K,Shave E,Baker K,Lua LHL

    更新日期:2018-12-01 00:00:00

  • Improvement of baculovirus as protein expression vector and as biopesticide by CRISPR/Cas9 editing.

    abstract::The clustered regularly interspaced short palindromic repeats (CRISPR) system-associated Cas9 endonuclease is a molecular tool that enables specific sequence editing with high efficiency. In this study, we have explored the use of CRISPR/Cas9 system for the engineering of baculovirus. We have shown that the delivering...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Pazmiño-Ibarra V,Mengual-Martí A,Targovnik AM,Herrero S

    更新日期:2019-11-01 00:00:00

  • Distribution of heparinase covalently immobilized to agarose: Experimental and theoretical studies.

    abstract::An immobilized enzyme reactor has been developed to remove heparin, the anticoagulant that is required in all extracorporeal devices for patients undergoing open-heart surgery or kidney dialysis. The device uses the enzyme heparinase (EC, which is covalently linked to agarose with cyanogen bromide. A critical...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Bernstein H,Yang VC,Langer R

    更新日期:1987-08-05 00:00:00

  • A continuous membrane bioreactor for ester synthesis in organic media: I. Operational characterization and stability.

    abstract::The feasibility of continuous ester synthesis in a membrane bioreactor (MBR) by a recombinant cutinase from Fusarium solani pisi was investigated, using the optimal conditions previously attained by medium engineering. The objective was to analyze the MBR behavior as a differential or an integral reactor. The main com...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Carvalho CM,Aires-Barros MR,Cabral JM

    更新日期:2001-01-20 00:00:00

  • A study of D-lactate and extracellular methylglyoxal production in lactate re-utilizing CHO cultures.

    abstract::In large-scale mammalian cell culture, the key toxic by-products assessed and monitored are lactate and ammonia. Often no distinction between the two isoforms of lactate is made. Here, we present profiles of both D- and L-lactate. D-Lactate is the end molecule of the methylglyoxal pathway. D-Lactate unlike L-lactate i...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Paoli T,Faulkner J,O'kennedy R,Keshavarz-Moore E

    更新日期:2010-09-01 00:00:00

  • Invertase biosynthesis by Saccharomyces carlsbergensis in batch and continuous cultures.

    abstract::The biosynthesis of invertase by Saccharomyces carlsbergensis LAM 1068 was studied in relation to its glucose effect at both unsteady and steady states of growth. Experimental correlations between the dilution rate and invertase specific activity (E/X) in chemostat cultures led to an optimum for the enzyme synthesis a...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Toda K

    更新日期:1976-08-01 00:00:00

  • Fibrous proteins: At the crossroads of genetic engineering and biotechnological applications.

    abstract::Fibrous proteins, such as silk, elastin and collagen are finding broad impact in biomaterial systems for a range of biomedical and industrial applications. Some of the key advantages of biosynthetic fibrous proteins compared to synthetic polymers include the tailorability of sequence, protein size, degradation pattern...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章,评审


    authors: Yigit S,Dinjaski N,Kaplan DL

    更新日期:2016-05-01 00:00:00

  • Analysis of the coupled transport, reaction, and deactivation phenomena in the immobilized glucose oxidase and catalase system.

    abstract::In a previous paper, the overall or macrokinetics of the immobilized glucose oxidase--catalase system has been presented. In this paper a detailed analysis of the interaction of diffusion and reaction in this system will be presented. The mathematical treatment includes two consecutive reactions with two-substrate kin...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Reuss M,Buchholz K

    更新日期:1979-11-01 00:00:00

  • Development of a novel adenovirus purification process utilizing selective precipitation of cellular DNA.

    abstract::The use of recombinant adenoviral vectors for vaccination and gene therapy requires the development of purification processes that are cost-effective, scalable, and capable of robust host cell DNA clearance. An adenovirus purification process was developed which incorporates selective precipitation of host cell DNA, e...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Goerke AR,To BC,Lee AL,Sagar SL,Konz JO

    更新日期:2005-07-05 00:00:00

  • Competition between Nitrospira spp. and Nitrobacter spp. in nitrite-oxidizing bioreactors.

    abstract::In this work the question was addressed if in nitrite-oxidizing activated sludge systems the environmental competition between Nitrobacter spp. and Nitrospira spp., which only recently has been discovered to play a role in these systems, is affected by the nitrite concentrations. Two parallel chemostats were inoculate...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Nogueira R,Melo LF

    更新日期:2006-09-05 00:00:00

  • Application of temperature-sensitive mutants for single-cell protein production.

    abstract::Cell-division-cycle, temperature-sensitive mutants of Saccharomyces cerevisiae were investigated as a means of altering the morphological characteristics and subsequent physical properties of single-cell protein (SCP). Strain 4471, harboring mutation cdc 4, formed a visible complex mass at the nonpermissive temperatur...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Miyasaka Y,Rha C,Sinskey AJ

    更新日期:1980-10-01 00:00:00

  • Development of liver microtissues with functional biliary ductular network.

    abstract::Liver tissue engineering aims to create transplantable liver grafts that can serve as substitutes for donor's livers. One major challenge in creating a fully functional liver tissue has been to recreate the biliary drainage in an engineered liver construct through integration of bile canaliculi (BC) with the biliary d...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Hafiz EOA,Bulutoglu B,Mansy SS,Chen Y,Abu-Taleb H,Soliman SAM,El-Hindawi AAF,Yarmush ML,Uygun BE

    更新日期:2021-01-01 00:00:00

  • Nisin-inducible secretion of a biologically active single-chain insulin analog by Lactococcus lactis NZ9000.

    abstract::Oral delivery of insulin to diabetic patients is highly desirable because it would be non-invasive and more closely mimic normal physiology, but this route of administration typically results in low bioavailability due to low pH and enzymatic degradation along the gastrointestinal tract. To explore an alternative appr...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ng DT,Sarkar CA

    更新日期:2011-08-01 00:00:00

  • Assessment of the in vivo biofunctionality of a biomimetic hybrid scaffold for osteochondral tissue regeneration.

    abstract::Chondral and osteochondral lesions represent one of the most challenging problems in the orthopedic field, as these types of injuries lead to disability and worsened quality of life for patients and have an economic impact on the healthcare system. The aim of this in vivo study was to develop a new tissue engineering ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Tschon M,Brogini S,Parrilli A,Bertoldi S,Silini A,Parolini O,Faré S,Martini L,Veronesi F,Fini M,Giavaresi G

    更新日期:2021-01-01 00:00:00

  • Novel non-viral vectors for gene delivery: synthesis of a second-generation library of mono-functionalized poly-(guanidinium)amines and their introduction into cationic lipids.

    abstract::The development of new gene delivery technologies is a prerequisite towards gene therapy clinical trials. Because gene delivery mediated by viral vectors remains of limited scope due to immunological and propagation risks, the development of new non-viral gene delivery systems is of crucial importance. We have synthes...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Byk G,Soto J,Mattler C,Frederic M,Scherman D

    更新日期:1998-04-01 00:00:00

  • Metabolically engineered Caldicellulosiruptor bescii as a platform for producing acetone and hydrogen from lignocellulose.

    abstract::The production of volatile industrial chemicals utilizing metabolically engineered extreme thermophiles offers the potential for processes with simultaneous fermentation and product separation. An excellent target chemical for such a process is acetone (Tb  = 56°C), ideally produced from lignocellulosic biomass. Caldi...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Straub CT,Bing RG,Otten JK,Keller LM,Zeldes BM,Adams MWW,Kelly RM

    更新日期:2020-08-08 00:00:00

  • Characterization of an on-line commercial fluorescence probe: modeling of the probe signal.

    abstract::A biosensor model was developed for a commercial NADH fluorescence probe to describe the single-frequency excitation and emission fluorescence behavior of an aqueous mixture of fluorophores. This model is essential in correlating the measured signals to the concentrations of fluorescent compounds in a bioreactor. In a...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Wang NS,Simmons MB

    更新日期:1991-12-20 00:00:00