Acetoclastic methanogenic activity measurement by a titration bioassay.

abstract：:Mammalian cell lines for recombinant protein production need to maintain productivity over extended cultivation times. Long-term stability studies are time and resource intensive, but are widely performed to identify and eliminate unstable candidates during cell line development. Production instability of manufacturin...

journal_title：Biotechnology and bioengineering

authors： Osterlehner A,Simmeth S,Göpfert U

更新日期：2011-11-01 00:00:00

abstract：:Biomass autoflocculation in outdoor algal cultures was found to be associated with increases of culture pH levels, due to CO(2) consumption by the algal photosynthetic activity. Under these alkaline conditions, some medium chemical ions precipitated together with the algal biomass. The chemical substances involved wit...

journal_title：Biotechnology and bioengineering

authors： Sukenik A,Shelef G

更新日期：1984-02-01 00:00:00

abstract：:The inhibitory effect of ethanol on yeast growth and fermentation has been studied for the strain Saccharomyces cerevisiae ATCC No. 4126 under anaerobic batch conditions. The results obtained reveal that there is no striking difference between the response of growth and ethanol fermentation. Two kinetic models are als...

journal_title：Biotechnology and bioengineering

authors： Luong JH

更新日期：1985-03-01 00:00:00

abstract：:A spinning cylindrical filter is often used to retain mammalian cells in a continuous perfusion bioreactor. This device, known as a spinfilter, has typically been with pore size smaller than the cell particles (single cells or aggregates) in order to achieve cell separation. For single cells in suspension, such an ope...

journal_title：Biotechnology and bioengineering

authors： Yabannavar VM,Singh V,Connelly NV

更新日期：1992-10-20 00:00:00

abstract：:Multivalent cations have been known to be important components of activated sludge floc structure due to their bridging ability of the negatively charged sites on the biopolymer network. Recently in batch systems it was found that excess concentration of monovalent cations led to the deterioration in settleability, de...

journal_title：Biotechnology and bioengineering

authors： Kara F,Gurakan GC,Sanin FD

更新日期：2008-06-01 00:00:00

abstract：:As part of an investigation into the use of biological nitrogen fixation for fertilizer ammonia production, continuous culture studies of respiration and nitrogen fixation in the aerobic bacteria Azotobacter vinelandii under oxygen-limited conditions were conducted. Respiration and growth rates followed Monod forms wi...

journal_title：Biotechnology and bioengineering

authors： Diluccio RC,Kirwan DJ

更新日期：1984-01-01 00:00:00

abstract：:A novel reaction system was developed to maximize the catalytic efficiency of chloroperoxidase (CPO, from Caldariomyces fumago) toward the oxidation of hydrocarbons. The reaction system consisted of an organic/aqueous emulsion comprising pure substrate and aqueous buffer supplemented with the surfactant dioctyl sulfos...

journal_title：Biotechnology and bioengineering

authors： Park JB,Clark DS

更新日期：2006-05-05 00:00:00

abstract：:The effect of protein concentration in partitioning in PEG/salt aqueous two-phase systems has been investigated. PEG 4000/phosphate systems in the presence of 0% w/w and 8.8% w/w NaCl have been evaluated using amyloglucosidase, subtilisin, and trypsin inhibitor. Also, a PEG 4000/phosphate system with 3% w/w NaCl was u...

journal_title：Biotechnology and bioengineering

authors： Schmidt AS,Andrews BA,Asenjo JA

更新日期：1996-06-20 00:00:00

abstract：:Anerobic biofilms with dominantly acidogenic bacteria were grown in fixed-bed recycle reactors. The influence of calcium concentration in the culture medium on biofilm mass accumulation, immobilized calcium concentration, and biofilm-specific activity was investigated. The results indicate that the biofilm mass accumu...

journal_title：Biotechnology and bioengineering

authors： Huang J,Pinder KL

更新日期：1995-02-05 00:00:00

abstract：:A simple methodology has been successfully employed to explain the solvent dependence of the substrate specificity of enzymes in organic media. This methodology, which does not require the knowledge of the enzyme structure and is thus applicable to lyophilized and other noncrystalline enzyme preparations, predicts tha...

journal_title：Biotechnology and bioengineering

authors： Wescott CR,Klibanov AM

更新日期：1997-11-05 00:00:00

abstract：:We report the isolation of mutant strains of the methylotrophic yeast Hansenula polymorpha that are able to efficiently oxidize ethanol to acetaldehyde in an intact cell system. The oxidation reaction is catalyzed by alcohol oxidase (AOX), a key enzyme in the methanol metabolic pathway that is typically present only i...

journal_title：Biotechnology and bioengineering

authors： Moroz OM,Gonchar MV,Sibirny AA

更新日期：2000-04-05 00:00:00

abstract：:The feasibility of NO3- removal by the synergistic action of a prevailing denitrifying anoxic methane oxidising (DAMO), and nitrate-reducing and sulfide-oxidising bacterial (NR-SOB) consortium, using CH4 and H2 S from biogas as electron donors in a biotrickling filter was investigated. The influence of NO3- concentrat...

journal_title：Biotechnology and bioengineering

authors： López JC,Porca E,Collins G,Pérez R,Rodríguez-Alija A,Muñoz R,Quijano G

更新日期：2017-03-01 00:00:00

abstract：:Hairy roots have the potential to produce a variety of valuable small and large molecules. The mist reactor is a gas phase bioreactor that has shown promise for low-cost culture of hairy roots. Using a newer, disposable culture bag, mist reactor performance was studied with two species, Artemisia annua L. and Arachis ...

journal_title：Biotechnology and bioengineering

authors： Sivakumar G,Liu C,Towler MJ,Weathers PJ

更新日期：2010-12-01 00:00:00

abstract：:The aim of this study was to determine the feasibility of using high pressure CO2 for sterilization of Ginseng powder, as an alternative method to conventional techniques such as gamma-irradiation and ethylene oxide. The Ginseng sample used in this study was originally contaminated with fungi and 5 x 10(7) bacteria/g ...

journal_title：Biotechnology and bioengineering

authors： Dehghani F,Annabi N,Titus M,Valtchev P,Tumilar A

更新日期：2009-02-01 00:00:00

abstract：:Three-dimensional cell culture models, such as spheroids, can be used in the process of the development of new anticancer agents because they are able to closely mimic the main features of human solid tumors, namely their structural organization, cellular layered assembling, hypoxia, and nutrient gradients. These prop...

journal_title：Biotechnology and bioengineering

authors： Nunes AS,Barros AS,Costa EC,Moreira AF,Correia IJ

更新日期：2019-01-01 00:00:00

abstract：:Polyhydroxyalkanoates (PHA) terployesters P(3HB-co-3HV-co-3HHx) consisting of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxyhexanoate (3HHx) were produced by wild-type Aeromonas hydrophila 4AK4, its recombinant harboring PHA synthesis genes phaPCJ encoding PHA binding protein phasin, PHA synthase, and...

journal_title：Biotechnology and bioengineering

authors： Zhang HF,Ma L,Wang ZH,Chen GQ

更新日期：2009-10-15 00:00:00

abstract：:The sustainability of autotrophic granular system performing partial nitritation and anaerobic ammonium oxidation (anammox) for complete nitrogen removal is impaired by the production of nitrous oxide (N2 O). A systematic analysis of the pathways and affecting parameters is, therefore, required for developing N 2 O mi...

journal_title：Biotechnology and bioengineering

authors： Chen X,Ni BJ,Sin G

更新日期：2019-06-01 00:00:00

abstract：:A microscale model for the transport and coupled reaction of microbes and chemicals in an idealized two-dimensional porous media has been developed. This model includes the flow, transport, and bioreaction of nutrients, electron acceptors, and microbial cells in a saturated granular porous media. The fluid and chemica...

journal_title：Biotechnology and bioengineering

authors： Dillon R,Fauci L

更新日期：2000-06-05 00:00:00

abstract：:Thermodynamic feasibility analysis (TFA) has been used as a tool capable of providing additional constraints to the mass balance-based methods of analysis of metabolic networks (e.g., flux balance analysis). Several publications have recently appeared in which TFA of different metabolic pathways from relatively simple...

journal_title：Biotechnology and bioengineering

authors： Vojinović V,von Stockar U

更新日期：2009-07-01 00:00:00

abstract：:An approach is presented for the stable covalent immobilization of proteins with a high retention of biological activity. First, chemical modification studies were used to establish enzyme structural and functional properties relevant to the covalent immobilization of an enzyme to agarose based supports. Heparinase wa...

journal_title：Biotechnology and bioengineering

authors： Leckband D,Langer R

更新日期：1991-02-05 00:00:00

abstract：:The biosynthesis of invertase by Saccharomyces carlsbergensis LAM 1068 was studied in relation to its glucose effect at both unsteady and steady states of growth. Experimental correlations between the dilution rate and invertase specific activity (E/X) in chemostat cultures led to an optimum for the enzyme synthesis a...

journal_title：Biotechnology and bioengineering

authors： Toda K

更新日期：1976-08-01 00:00:00

abstract：:Methyl parathion hydrolase (MPH) has been displayed on the surface of microorganisms for the first time using only N- and C-terminal domains of the ice nucleation protein (INPNC) from Pseudomonas syringae INA5 as an anchoring motif. A shuttle vector pINCM coding for INPNC-MPH was constructed and used to target MPH ont...

journal_title：Biotechnology and bioengineering

authors： Yang C,Cai N,Dong M,Jiang H,Li J,Qiao C,Mulchandani A,Chen W

更新日期：2008-01-01 00:00:00

abstract：:Short-chain carboxylic acids generated by various mixed- or pure-culture fermentation processes have been considered valuable precursors for production of bioalcohols. While conversion of carboxylic acids into alcohols is routinely performed with catalytic hydrogenation or with strong chemical reducing agents, here, a...

journal_title：Biotechnology and bioengineering

authors： Perez JM,Richter H,Loftus SE,Angenent LT

更新日期：2013-04-01 00:00:00

abstract：:Linear relations among net conversion rates of primary metabolites in fermentation processes can be determined by using the macroscopic or pathway balancing methods. It is shown that the process observability can be improved by incorporating both the abiotic proton balance and the pH control reagent addition rate meas...

journal_title：Biotechnology and bioengineering

authors： Siano SA

更新日期：1996-12-20 00:00:00

abstract：:The production of beta-galactosidase from the E. coli phoA-lacZ fusion gene was studied to compare the gene expression behavior of two cloning methods: insertion to multicopy plasmids and integration into host cell's chromosome. The chromosome-integrating strain showed more tight control of fusion gene expression leve...

journal_title：Biotechnology and bioengineering

authors： Shin PK,Seo JH

更新日期：1990-12-20 00:00:00

abstract：:Geotrichum candidum was cultivated at the surface of solid model media containing peptone to simulate the composition of Camembert cheese. The surface growth of G. candidum induced the diffusion of substrates from the core to the rind and the diffusion of produced metabolites from the rind to the core. In the range of...

journal_title：Biotechnology and bioengineering

authors： Aldarf M,Fourcade F,Amrane A,Prigent Y

更新日期：2004-07-05 00:00:00

abstract：:An unadapted mixed methanogenic sludge transformed formaldehyde into methanol and formate. The methanol to formate ratio obtained was 1:1. Formaldehyde conversion proceeded without any lag phase, suggesting the constitutive character of the formaldehyde conversion enzymes involved. Because the rate of formaldehyde con...

journal_title：Biotechnology and bioengineering

authors： Gonzalez-Gil G,Kleerebezem R,Lettinga G

更新日期：2002-08-05 00:00:00

abstract：:A microarray presenting glycerol nanodroplets of fluorogenic peptide substrates was used as a biosensor for the detection of multiple enzyme activities within human plasma. Using 10 different plasma proteases (kallikrein, factor XIIa, factor XIa, factor IXa, factor VIIa, factor Xa, thrombin, activated protein C, uPA a...

journal_title：Biotechnology and bioengineering

authors： Gosalia DN,Denney WS,Salisbury CM,Ellman JA,Diamond SL

更新日期：2006-08-20 00:00:00

abstract：:Bioaffinity interactions have been, and continue to be, successfully adapted from nature for use in separation and detection applications. It has been previously reported that the magnetophoretic mobility of labeled cells show a saturation type phenomenon as a function of the concentration of the free antibody-magneti...

journal_title：Biotechnology and bioengineering

authors： Zhang H,Williams PS,Zborowski M,Chalmers JJ

更新日期：2006-12-05 00:00:00

abstract：:A microarray targeting four sequenced strains in the Dehalococcoides (Dhc) genus was used to analyze gene expression in a robust long-term trichloroethene (TCE)-degrading microbial community (designated ANAS) during feeding cycles that involve conditions of periodic substrate supply. The Dhc transcriptome was examined...

journal_title：Biotechnology and bioengineering

authors： West KA,Lee PK,Johnson DR,Zinder SH,Alvarez-Cohen L

更新日期：2013-05-01 00:00:00