Promoter methylation and transgene copy numbers predict unstable protein production in recombinant Chinese hamster ovary cell lines.

abstract：:The transesterification reaction of N-acetyl-L-phenylalanine ethyl ester with 1-propanol catalyzed by alpha-chymotrypsin was examined in the ionic liquids 1-butyl-3-methylimidazolium hexafluorophosphate ([bmim][PF(6)]) and 1-octyl-3-methylimidazolium hexafluorophosphate ([omim][PF(6)]), and in combination with supercr...

journal_title：Biotechnology and bioengineering

authors： Laszlo JA,Compton DL

更新日期：2001-10-20 00:00:00

abstract：:The results of an initial study of enzymatic catalysis in metastable supersaturated solutions of carbohydrates are presented. It has been shown that such solutions, formed in the presence of small amounts of water and alcohol as plasticizers, are sufficiently stable under ambient conditions to enable enzymatic transfo...

journal_title：Biotechnology and bioengineering

authors： Millqvist-Fureby A,Gill IS,Vulfson EN

更新日期：1998-10-20 00:00:00

abstract：:To build complex genetic networks with predictable behaviors, synthetic biologists use libraries of modular parts that can be characterized in isolation and assembled together to create programmable higher-order functions. Characterization experiments and computational models for gene regulatory parts operating in iso...

journal_title：Biotechnology and bioengineering

authors： Westbrook A,Tang X,Marshall R,Maxwell CS,Chappell J,Agrawal DK,Dunlop MJ,Noireaux V,Beisel CL,Lucks J,Franco E

更新日期：2019-05-01 00:00:00

abstract：:Anaerobic digestion of biological organic particulates to methane has been described by a structured mathematical model based on multiple-reaction stoichiometry, conventional material balances, and liquid phase equilibrium chemistry. A general stoichiometric treatment for any set of multiple biological reactions is de...

journal_title：Biotechnology and bioengineering

authors： Bryers JD

更新日期：1985-05-01 00:00:00

abstract：:Kluyveromyces marxianus UCD (FST) 55-82 cells were immobilized in Na alginate beads and used in a packed-bed bioreactor system for the continuous production of ethanol from the extract of Jerusalem artichoke tubers. Volumetric ethanol productivities of 104 and 80 g ethanol/ L/h were obtained at 80 and 92% sugar utiliz...

journal_title：Biotechnology and bioengineering

authors： Margaritis A,Bajpai P

更新日期：1982-07-01 00:00:00

abstract：:An experimental study was undertaken to quantify the effects of infection cell density, medium condition, and surface aeration on recombinant protein yields in insect cells. In the absence of surface aeration and fresh medium, insect cells generated higher product yields (on a per cell basis) when infected with recomb...

journal_title：Biotechnology and bioengineering

authors： Lindsay DA,Betenbaugh MJ

更新日期：1992-03-15 00:00:00

abstract：:Acetate accumulation is a common problem observed in aerobic high cell density Escherichia coli cultures. A previous report has hypothesized that the glyoxylate shunt is active in a low acetate producer, E. coli BL21, and inactive in a high acetate producer, JM109. To further investigate this hypothesis, we now develo...

journal_title：Biotechnology and bioengineering

authors： Noronha SB,Yeh HJ,Spande TF,Shiloach J

更新日期：2000-05-05 00:00:00

abstract：:Bovine pancreatic trypsin was treated with ethylene glycol bis(succinic acid N-hydroxysuccinimide ester). Approximately 8 of 14 lysines per trypsin molecule were modified. This derivative (EG trypsin) was more stable than native between 30 degrees and 70 degrees C: T50 values were 59 degrees C and 46 degrees C, respec...

journal_title：Biotechnology and bioengineering

authors： Murphy A,O Fágáin C

更新日期：1998-05-20 00:00:00

abstract：:We investigated the enzymic activity of bilirubin oxidase when it is suspended as a lyophilized powder in a low-water system. The enzyme required buffer salts and a source of water to show activity. This study investigated the complete range of water thermodynamic activity (a(w)) by combining the use of salt hydrates ...

journal_title：Biotechnology and bioengineering

authors： Alston MJ,Freedman RB

更新日期：2002-03-20 00:00:00

abstract：:The possibility of enhancing the biomass productivity of a continuous culture of Saccharomyces cerevisiae growing on a glucose-limited medium is addressed. An unstructured Monod-type model is first identified using steady-state data. The culture is subjected to step changes in dilution rate, and it is seen that the Mo...

journal_title：Biotechnology and bioengineering

authors： Abulesz EM,Lyberatos G

更新日期：1989-09-01 00:00:00

abstract：:A major challenge in downstream purification of monoclonal antibodies (mAb) is the removal of host cell proteins (HCPs). Previous studies have shown that cell culture conditions significantly impact the HCP content at harvest. However, it is currently unclear how process conditions affect physiological changes in the ...

journal_title：Biotechnology and bioengineering

authors： Goey CH,Tsang JMH,Bell D,Kontoravdi C

更新日期：2017-12-01 00:00:00

abstract：:The sensitivity of a conceptual model of a foam emulsion bioreactor (FEBR) used for the control of toluene vapors in air was examined. Model parametric sensitivity studies showed which parameters affect the removal of toluene (as model pollutant) in the FEBR the most significantly, and enabled definition of the limits...

journal_title：Biotechnology and bioengineering

authors： Kan E,Deshusses MA

更新日期：2009-02-15 00:00:00

abstract：:Fluorotelomer alcohols [FTOHs, F(CF(2))(n) CH(2)CH(2)OH, n = 4, 6, and 8] are emerging environmental contaminants. Biotransformation of FTOHs by mixed bacterial cultures has been reported; however, little is known about the microorganisms responsible for the biotransformation. Here we reported biotransformation of FTO...

journal_title：Biotechnology and bioengineering

authors： Kim MH,Wang N,McDonald T,Chu KH

更新日期：2012-12-01 00:00:00

abstract：:RNA viruses are capable of undergoing extremely rapid evolution due to their high rates of reproduction, small genome size, and a high frequency of spontaneous mutagenesis. Here we demonstrate that a virus-like, evolutionary state can be created by propagating a phagemid population in a hypermutator strain of Escheric...

journal_title：Biotechnology and bioengineering

authors： Long-McGie J,Liu AD,Schellenberger V

更新日期：2000-04-05 00:00:00

abstract：:Yeast alcohol dehydrogenase (ADH) solutions (approximately 1 mg/ml, pH 7) were sheared in a coaxial cylindrical viscometer. This was fitted with a lid sealing the contents from the atmosphere and preventing evaporation. At 30 degrees C after a total of 5 hr intermittent shearing at 683 sec-1 no losses of activity were...

journal_title：Biotechnology and bioengineering

authors： Thomas CR,Nienow AW,Dunnill P

更新日期：1979-12-01 00:00:00

abstract：:The kinetics in batch culture of the acetone butanol fermentation by Clostridium acetobutylicum is compared on glucose, xylose, and mixtures of both sugars. The fastest initial growth and transition from an acid to a solvent metabolism occurs on glucose, with a final 62 g/L glucose conversion. On xylose, an initial sl...

journal_title：Biotechnology and bioengineering

authors： Fond O,Engasser JM,Matta-El-Amouri G,Petitdemange H

更新日期：1986-02-01 00:00:00

abstract：:Penicillinase (beta-lactamase I, EC 3.5.2.6) secreted by Bacillus cereus, strain 569/H, was covalently attached to aminoethyl cellulose via glutaraldehyde. The immobilized derivative shows increased thermostability and decreased susceptibility to conformational changes induced by certain substrates of penicillinase. T...

journal_title：Biotechnology and bioengineering

authors： Klemes Y,Citri N

更新日期：1979-05-01 00:00:00

abstract：:The laminate consisted of several polymer layers, aluminium, and one cellulose-based layer containing the active enzymatic system (e.g., glucose oxidase, catalase, glucose, and CaCO3). During the industrial lamination process, the enzyme layer was exposed to three temperature spikes up to 325 degrees C without signifi...

journal_title：Biotechnology and bioengineering

authors： Andersson M,Andersson T,Adlercreutz P,Nielsen T,Hörnsten EG

更新日期：2002-07-05 00:00:00

abstract：:The enantiocatalytic performance of immobilized lipase in an emulsion membrane reactor using stable emulsion prepared by membrane emulsification technology was studied. The production of optical pure (S)-naproxen from racemic naproxen methyl ester was used as a model reaction system. The O/W emulsion, containing the s...

journal_title：Biotechnology and bioengineering

authors： Giorno L,Li N,Drioli E

更新日期：2003-12-20 00:00:00

abstract：:The morphology of mycelial fungi in liquid culture effects culture rheology and this in turn may affect product yield. It is therefore important to understand how environmental factors influence mycelial morphology and this paper describes the effect of dilution rate on two strains of Fusarium graminearum, the relativ...

journal_title：Biotechnology and bioengineering

authors： Wiebe MG,Trinci AP

更新日期：1991-06-05 00:00:00

abstract：:A microarray targeting four sequenced strains in the Dehalococcoides (Dhc) genus was used to analyze gene expression in a robust long-term trichloroethene (TCE)-degrading microbial community (designated ANAS) during feeding cycles that involve conditions of periodic substrate supply. The Dhc transcriptome was examined...

journal_title：Biotechnology and bioengineering

authors： West KA,Lee PK,Johnson DR,Zinder SH,Alvarez-Cohen L

更新日期：2013-05-01 00:00:00

abstract：:A method was developed for obtaining high signal-to-noise 13C NMR spectra of intracellular compounds in metabolically active cultured cells. The method allows TCA cycle labeling kinetics to be determined in real time without significant oxygen transport limitations. Cells were immobilized on the surface of nonporous m...

journal_title：Biotechnology and bioengineering

authors： Mancuso A,Beardsley NJ,Wehrli S,Pickup S,Matschinsky FM,Glickson JD

更新日期：2004-09-30 00:00:00

abstract：:Poly lactic acid (PLA) based plastics is renewable, bio-based, and biodegradable. Although present day PLA is composed of mainly L-LA, an L- and D- LA copolymer is expected to improve the quality of PLA and expand its use. To increase the number of thermotolerant microbial biocatalysts that produce D-LA, a derivative ...

journal_title：Biotechnology and bioengineering

authors： Awasthi D,Wang L,Rhee MS,Wang Q,Chauliac D,Ingram LO,Shanmugam KT

更新日期：2018-02-01 00:00:00

abstract：:The cellulase production from immobilized Trichoderma reesei composites prepared by radiation polymerization at low temperature was studied. The production of cellulase from the cells irradiated by radiation was slightly retarded at the initial stage of the culture, but was immediately recovered. The production of cel...

journal_title：Biotechnology and bioengineering

authors： Kumakura M,Kaetsu I,Nisizawa K

更新日期：1984-01-01 00:00:00

abstract：:This article describes a method for the chemical immobilization of concanavalin A (Con A) on the inside wall of a single hollow cellulose fiber for use in glucose affinity sensor. Periodate oxidation of cellulose fiber followed by a spacer for Con A attachment was deemed to be the most optimal procedure for achieving ...

journal_title：Biotechnology and bioengineering

authors： Srinivasan KR,Mansouri S,Schultz JS

更新日期：1986-02-01 00:00:00

abstract：:One potential advantage of an extremely thermophilic metabolic engineering host (T opt ≥ 70°C) is facilitated recovery of volatile chemicals from the vapor phase of an active fermenting culture. This process would reduce purification costs and concomitantly alleviate toxicity to the cells by continuously removing solv...

journal_title：Biotechnology and bioengineering

authors： Zeldes BM,Straub CT,Otten JK,Adams MWW,Kelly RM

更新日期：2018-12-01 00:00:00

abstract：:In a stirred culture of hybridoma cells, the effects of serum reduction from 2.5% to 0% on growth and monoclonal antibody production have been investigated. The shear sensitivity of cells from the same culture has been tested in a bubble column. Serum reduction does not greatly affect viable-cell concentrations, but c...

journal_title：Biotechnology and bioengineering

authors： van der Pol L,Bakker WA,Tramper J

更新日期：1992-06-05 00:00:00

abstract：:The discovery of denitrifying anaerobic methane oxidation (DAMO) processes enables the complete nitrogen removal from wastewater by utilizing the methane produced on site from anaerobic digesters. This model-based study investigated the mechanisms and operational window for efficient nitrogen removal by coupling nitri...

journal_title：Biotechnology and bioengineering

authors： Chen X,Guo J,Xie GJ,Yuan Z,Ni BJ

更新日期：2016-05-01 00:00:00

abstract：:Gluconic acid and sorbitol were simultaneously produced from glucose and Jerusalem artichoke using a glucose-fructose oxidoreductase of Zymomonas mobilis and inulinase. Inulinase was immobilized on chitin by cross-linking with glutaraldehyde. Cells of Z. mobilis permeabilized with toluene were coimmobilized with chiti...

journal_title：Biotechnology and bioengineering

authors： Kim DM,Kim HS

更新日期：1992-02-05 00:00:00

abstract：:Streamline Direct CST I is a new type of ion exchanger with multi-modal functional groups, specially designed for an expanded bed adsorption (EBA) process, which can capture directly the proteins from the high ionic strength feedstocks with a high binding capacity. In this study, an experimental study is carried out f...

journal_title：Biotechnology and bioengineering

authors： Li P,Xiu G,Mata VG,Grande CA,Rodrigues AE

更新日期：2006-08-20 00:00:00