Continuous production of gluconic acid and sorbitol from Jerusalem artichoke and glucose using an oxidoreductase of Zymomonas mobilis and inulinase.

abstract：:Breast cancer cells experience a range of shear stresses in the tumor microenvironment (TME). However most current in vitro three-dimensional (3D) models fail to systematically probe the effects of this biophysical stimuli on cancer cell metastasis, proliferation, and chemoresistance. To investigate the roles of shear...

journal_title：Biotechnology and bioengineering

authors： Novak CM,Horst EN,Taylor CC,Liu CZ,Mehta G

更新日期：2019-11-01 00:00:00

abstract：:Chromatography is the method of choice for the separation of proteins, at both analytical and preparative scale. Orthogonal purification strategies for industrial use can easily be implemented by combining different modes of adsorption. Nevertheless, with flexibility comes the freedom of choice and optimal conditions ...

journal_title：Biotechnology and bioengineering

authors： Dismer F,Hansen S,Oelmeier SA,Hubbuch J

更新日期：2013-03-01 00:00:00

abstract：:There has been much effort exerted to reduce one carbon (C1) gas emission to address climate change. As one promising way to more conveniently utilize C1 gas, several technologies have been developed to convert C1 gas into useful chemicals such as formic acid (FA). In this study, systems metabolic engineering was util...

journal_title：Biotechnology and bioengineering

authors： Ahn JH,Bang J,Kim WJ,Lee SY

更新日期：2017-12-01 00:00:00

abstract：:The cometabolic transformation of 2,4,6-trinitrotoluene (TNT) by an immobilized Phanerochaete chrysosporium culture was investigated under different TNT and/or glycerol feeding conditions in a 5-L reactor. In the fed-batch feeding mode, as a result of four spiking events at an average feeding rate of 20 mg TNT L(-1) d...

journal_title：Biotechnology and bioengineering

authors： Rho D,Hodgson J,Thiboutot S,Ampleman G,Hawari J

更新日期：2001-05-20 00:00:00

abstract：:The analysis of a continuous, aerobic, fixed-film bioreactor is performed by simulating the behavior of penicillin production in a three-phase fluidized bed. Rigorous mathematical models are developed for a fluidized-bed fermentor in which bioparticles are fluidized by the liquid medium and air. The steady-state perfo...

journal_title：Biotechnology and bioengineering

authors： Park Y,Davis ME,Wallis DA

更新日期：1984-05-01 00:00:00

abstract：:Addition of Tween 85 to aqueous suspensions of Anabaena variabilis induced photosynthetic evolution of hydrogen over a time span of several weeks: As much as 148 nmol H(2)/h . mg dry weight was produced in the first week by a suspension containing 4.2 mg dry weight of cells and 77 mM Tween 85. The chemical structure o...

journal_title：Biotechnology and bioengineering

authors： Famiglietti M,Hochkoeppler A,Luisi PL

更新日期：1993-10-01 00:00:00

abstract：:Antimicrobial peptides (AMPs) kill microbes by non-specific membrane permeabilization, making them ideal templates for designing novel peptide-based antibiotics that can combat multi-drug resistant pathogens. For maximum efficacy in vivo and in vitro, AMPs must be biocompatible, salt-tolerant and possess broad-spectru...

journal_title：Biotechnology and bioengineering

authors： Saravanan R,Li X,Lim K,Mohanram H,Peng L,Mishra B,Basu A,Lee JM,Bhattacharjya S,Leong SS

更新日期：2014-01-01 00:00:00

abstract：:Pretreatment of rice straw by using renewable cholinium amino acids ionic liquids ([Ch][AA] ILs)-water mixtures and the subsequent enzymatic hydrolysis of the residues were conducted in the present work. Of the eight mixtures composed of ILs and water, most were found to be effective for rice straw pretreatment. After...

journal_title：Biotechnology and bioengineering

authors： Hou XD,Li N,Zong MH

更新日期：2013-07-01 00:00:00

abstract：:Bovine pancreatic trypsin was treated with ethylene glycol bis(succinic acid N-hydroxysuccinimide ester). Approximately 8 of 14 lysines per trypsin molecule were modified. This derivative (EG trypsin) was more stable than native between 30 degrees and 70 degrees C: T50 values were 59 degrees C and 46 degrees C, respec...

journal_title：Biotechnology and bioengineering

authors： Murphy A,O Fágáin C

更新日期：1998-05-20 00:00:00

abstract：:Buddleja davidii is a unique biomass that has many attractive agroenergy features, especially its wide range of growth habitat. The anatomical characteristics of B. davidii were investigated before and after ethanol organosolv pretreatment (one of the leading pretreatment technologies) in order to further understand t...

journal_title：Biotechnology and bioengineering

authors： Hallac BB,Ray M,Murphy RJ,Ragauskas AJ

更新日期：2010-12-01 00:00:00

abstract：:We report the isolation of mutant strains of the methylotrophic yeast Hansenula polymorpha that are able to efficiently oxidize ethanol to acetaldehyde in an intact cell system. The oxidation reaction is catalyzed by alcohol oxidase (AOX), a key enzyme in the methanol metabolic pathway that is typically present only i...

journal_title：Biotechnology and bioengineering

authors： Moroz OM,Gonchar MV,Sibirny AA

更新日期：2000-04-05 00:00:00

abstract：:A model that was used to describe toxicity from high concentrations of chlorinated aliphatic hydrocarbons (CAHs) on reductively dechlorinating cultures in batch reactors (Sabalowsky and Semprini (in press)) was extended here to simulate observations in continuous flow suspended and attached growth reactors. The reduct...

journal_title：Biotechnology and bioengineering

authors： Sabalowsky AR,Semprini L

更新日期：2010-10-15 00:00:00

abstract：:A low-density, porous chitosan/poly-(dl-lactide-co-glycolide) (PLGA) microparticle composite scaffold was produced by thermally induced phase separation followed by lyophilization, to provide a bicontinuous microstructure potentially suitable for tissue engineering and locally controlled drug release. PLGA particles w...

journal_title：Biotechnology and bioengineering

authors： Nygaard JV,Andersen MØ,Howard KA,Foss M,Bünger C,Kjems J,Besenbacher F

更新日期：2008-07-01 00:00:00

abstract：:Measurement of the surface roughness and thickness of biological films is laborious and usually destructive, thus hampering research in this area. We developed a laser triangulation sensor (LTS) set-up for the fast and nondestructive measurement of these biofilm parameters during growth. Using LTS measurements, the mo...

journal_title：Biotechnology and bioengineering

authors： Okkerse WJ,Ottengraf SP,Osinga-Kuipers B

更新日期：2000-12-20 00:00:00

abstract：:All bacteria release organic compounds called soluble microbial products (SMP) as a part of their normal metabolism. In photobioreactor (PBR) settings, SMP produced by cyanobacteria represent a major pool of carbon and electrons available to heterotrophic bacteria. Thus, SMP in PBRs are a major driver for the growth o...

journal_title：Biotechnology and bioengineering

authors： Zevin AS,Nam T,Rittmann B,Krajmalnik-Brown R

更新日期：2015-09-01 00:00:00

abstract：:Hairy roots grow quickly, reach high densities, and can produce significant amounts of secondary metabolites, yet their scale-up to bioreactors remains challenging. Artemisia annua produces a rich array of terpenoids, including the sesquiterpene, artemisinin, and transformed roots of this species provide a good model ...

journal_title：Biotechnology and bioengineering

authors： Souret FF,Kim Y,Wyslouzil BE,Wobbe KK,Weathers PJ

更新日期：2003-09-20 00:00:00

abstract：:We present an evaluation of the qualitative and quantitative effects that high concentrations of benzene and toluene have on the growth rate of several pure cultures that use these compounds as their sole carbon and energy source. The cultures employed were five widely studied environmental isolates: Pseudomonas putid...

journal_title：Biotechnology and bioengineering

authors： Alagappan G,Cowan R

更新日期：2003-09-30 00:00:00

abstract：:Tissue engineering devices as in vitro cell culture systems in scaffolds has encountered the bottleneck due to their much lower cell functions than real tissues/organs in vivo. Such situation has been improved in some extent by mimicking the cell microenvironments in vivo from either chemical or physical ways. However...

journal_title：Biotechnology and bioengineering

authors： Shen C,Meng Q,Zhang G

更新日期：2013-08-01 00:00:00

abstract：:A flow cytometric kinetic study of hybridoma growth and monoclonal antibody production is presented, along with the influence of glutamine on intracellular responses such as (relative) cell size, and cell RNA and total protein content. Specific findings are: (1) RNA content remained constant throughout the growth phas...

journal_title：Biotechnology and bioengineering

authors： Dalili M,Ollis DF

更新日期：1990-06-05 00:00:00

abstract：:James (Jay) E. Bailey was a pioneer in biotechnology and biochemical engineering. During his 30 years in academia he made seminal contributions to many fields of chemical engineering science, including catalysis and reaction engineering, bioprocess engineering, mathematical modeling of cellular processes, recombinant ...

journal_title：Biotechnology and bioengineering

authors： Dordick JS,Khosla C

更新日期：2002-09-05 00:00:00

abstract：:Helical-blade solids mixers have a large potential as bioreactors for solid-state fermentation (SSF). Fundamental knowledge of the flow and mixing behavior is required for robust operation of these mixers. In this study predictions of a discrete particle model were compared to experiments with colored wheat grain part...

journal_title：Biotechnology and bioengineering

authors： Schutyser MA,Briels WJ,Rinzema A,Boom RM

更新日期：2003-10-05 00:00:00

abstract：:The twin arginine translocation (Tat) pathway occurs naturally in E. coli and has the distinct ability to translocate folded proteins across the inner membrane of the cell. It has the potential to export commercially useful proteins that cannot be exported by the ubiquitous Sec pathway. To better understand the biopro...

journal_title：Biotechnology and bioengineering

authors： Branston SD,Matos CF,Freedman RB,Robinson C,Keshavarz-Moore E

更新日期：2012-04-01 00:00:00

abstract：:A model is proposed that accounts for the decreases in yield which occur in chemostat cultures of mesophilic yeasts at superoptimal growth temperatures. Two yield depressing effects were identified, one due to increased maintenance requirements by the viable fraction of the population, the other due to energy substrat...

journal_title：Biotechnology and bioengineering

authors： Van Uden N,Madeira-Lopes A

更新日期：1976-06-01 00:00:00

abstract：:The enantiocatalytic performance of immobilized lipase in an emulsion membrane reactor using stable emulsion prepared by membrane emulsification technology was studied. The production of optical pure (S)-naproxen from racemic naproxen methyl ester was used as a model reaction system. The O/W emulsion, containing the s...

journal_title：Biotechnology and bioengineering

authors： Giorno L,Li N,Drioli E

更新日期：2003-12-20 00:00:00

abstract：:The development of autoantibodies against factor VIII represents one of the major complications in the treatment of hemophilia A patients. We have employed a novel library system to obtain peptides that specifically neutralize the interaction between factor VIII and these inhibitors. The random peptides are presented ...

journal_title：Biotechnology and bioengineering

authors： Mersich C,Billes W,Pabinger I,Jungbauer A

更新日期：2007-09-01 00:00:00

abstract：:The objective of this work was to relate macroscopically measurable on-line fermentation parameters such as dissolved oxygen, off-gas oxygen and carbon dioxide, and cell mass, to the controlled production of key intracellular enzymes under carbon limited conditions. Both batch and perturbed batch aerobic fermentations...

journal_title：Biotechnology and bioengineering

authors： Pih N,De Bernardez E,Dhurjati P

更新日期：1988-03-01 00:00:00

abstract：:A novel acetone-butanol production process was developed which integrates a repeated fed-batch fermentation with continuous product removal and cell recycle. The inhibitory product concentrations of the fermentation by Clostridium acetobutylicum were reduced by the simultaneous extraction process using polyvinylpyridi...

journal_title：Biotechnology and bioengineering

authors： Yang X,Tsao GT

更新日期：1995-08-20 00:00:00

abstract：:This article describes the synthesis and regulation of beta(1-3)glucanase and protease enzymes from the cell lytic system of Oerskovia xanthineolytica LL-G109 in continuous culture using different concentrations of carbon source (glucose) and inducer (glucan). These two enzyme activities are the main components of a l...

journal_title：Biotechnology and bioengineering

authors： Andrews BA,Asenjo JA

更新日期：1987-10-05 00:00:00

abstract：:Soluble steel factor (SF) is a potent stimulator of hematopoietic progenitor cell proliferation in vitro, and cytokine combinations that include SF can support extensive expansions of hematopoietic cells. Recently, we showed that very primitive progenitor cells from normal human bone marrow require exposure to very hi...

journal_title：Biotechnology and bioengineering

authors： Zandstra PW,Jervis E,Haynes CA,Kilburn DG,Eaves CJ,Piret JM

更新日期：1999-05-20 00:00:00

abstract：:The human monoclonal antibody (humAb) 2F5 is a potent candidate for immunotherapy of HIV-1. The xenohybridoma derived humAb 2F5 is of IgG3 kappa isotype. Generally, IgG1 isotype has a longer half-life (beta-clearance) in humans than IgG3. Therefore the isotype was switched to an IgG1 by ligation of the 2F5 heavy chain...

journal_title：Biotechnology and bioengineering

authors： Kunert R,Steinfellner W,Purtscher M,Assadian A,Katinger H

更新日期：2000-01-05 00:00:00