Abstract:
:All bacteria release organic compounds called soluble microbial products (SMP) as a part of their normal metabolism. In photobioreactor (PBR) settings, SMP produced by cyanobacteria represent a major pool of carbon and electrons available to heterotrophic bacteria. Thus, SMP in PBRs are a major driver for the growth of heterotrophic bacteria, and understanding the distribution of SMP in PBRs is an important step toward proper management of PBR microbial communities. Here, we analyzed the SMP and microbial communities in two Synechocystis sp. PCC6803-based PBRs. The first PBR (PBRP0) became phosphate limited after several days of operation, while the second PBR (PBRP+) did not have phosphate limitation. Heterotrophic bacteria were detected in both PBRs, but PBRP0 had a much higher proportion of heterotrophic bacteria than PBRP+. Furthermore, PBRP+ had greater biomass production and lower SMP production per unit biomass than PBRP0. Carbohydrates that were most likely derived from hydrolysis of extracellular polymeric substances (EPS) dominated the SMP in PBRP0, while products resulting from cell lysis or decay dominated the SMP in PBRP+. Together, our data support that maintaining phosphate availability in Synechocystis-based PBRs is important for managing SMP and, thus, the heterotrophic community.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Zevin AS,Nam T,Rittmann B,Krajmalnik-Brown Rdoi
10.1002/bit.25602subject
Has Abstractpub_date
2015-09-01 00:00:00pages
1761-9issue
9eissn
0006-3592issn
1097-0290journal_volume
112pub_type
杂志文章abstract::The results from this study demonstrate that neutral resins can selectively concentrate specific indole alkaloids from dilute aqueous mixtures. Adsorption was observed to provide a one to two order of magnitude improvement in concentrating these alkaloids, as compared to an equivalent single-staged extraction. Since t...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260310905
更新日期:1988-06-05 00:00:00
abstract::A sequencing batch membrane biofilm reactor (SBMBfR) was developed for simultaneous carbon, nitrogen, and phosphorus removal from wastewater. This reactor was composed of two functional parts: (1) a gas-permeable membrane on which a nitrifying biofilm formed and (2) a bulk solution in which bacteria, mainly denitrifyi...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20887
更新日期:2006-07-05 00:00:00
abstract::Current animal and 2-D cell culture models employed in metastasis research and drug discovery remain poor mimics of human cancer physiology. Here we describe a "metastasis-on-a-chip" system allowing real time tracking of fluorescent colon cancer cells migrating from hydrogel-fabricated gut constructs to downstream liv...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25950
更新日期:2016-09-01 00:00:00
abstract::The objective of this work was to relate macroscopically measurable on-line fermentation parameters such as dissolved oxygen, off-gas oxygen and carbon dioxide, and cell mass, to the controlled production of key intracellular enzymes under carbon limited conditions. Both batch and perturbed batch aerobic fermentations...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260310406
更新日期:1988-03-01 00:00:00
abstract::Pretreatment of rice straw by using renewable cholinium amino acids ionic liquids ([Ch][AA] ILs)-water mixtures and the subsequent enzymatic hydrolysis of the residues were conducted in the present work. Of the eight mixtures composed of ILs and water, most were found to be effective for rice straw pretreatment. After...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24862
更新日期:2013-07-01 00:00:00
abstract::Three cell lines, that is, the human breast cancer cell line (MCF-7) and the human mammary epithelial cell line (S-1) and its malignant form (T4-2) were embedded in a reconstituted basement membrane (Matrigel) that had 20-nL pyramid-shaped silicon microstructures. The proliferative behavior of the MCF-7 cells was depe...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21274
更新日期:2007-06-15 00:00:00
abstract::Reduced downstream costs, together with high purity recovery of polyhydroxyalkanoate (PHA), will accelerate the commercialization of high quality PHA-based products. In this work, a process was designed for effective recovery of the copolymer poly(hydroxybutyrate-co-hydroxyhexanoate) (P(HB-co-HHx)) containing high lev...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24713
更新日期:2013-02-01 00:00:00
abstract::The results of an initial study of enzymatic catalysis in metastable supersaturated solutions of carbohydrates are presented. It has been shown that such solutions, formed in the presence of small amounts of water and alcohol as plasticizers, are sufficiently stable under ambient conditions to enable enzymatic transfo...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:1998-10-20 00:00:00
abstract::Uranium contamination is a serious concern at several sites motivating the development of novel treatment strategies such as the Geobacter-mediated reductive immobilization of uranium. However, this bioremediation strategy has not yet been optimized for the sustained uranium removal. While several reactive-transport m...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.23096
更新日期:2011-07-01 00:00:00
abstract::High methanol concentrations have a negative effect on the growth rate and the biomass yield of growth transients induced by methanol pulses in continuous cultures of Methylomonas L3. The physiological basis of this effect is investigated by measuring the effect of the methanol pulse on the cell energy charge (EC) and...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260290110
更新日期:1987-01-01 00:00:00
abstract::Axial and radial oxygen depletion are believed to be critical scale-limiting factors in the design of cell culture hollow fiber bioreactors. A mathematical analysis of oxygen depletion has been performed in order to develop effectiveness factor plots to aid in the scaling of hollow fiber bioreactors with cells immobil...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260370112
更新日期:1991-01-05 00:00:00
abstract::Understanding the regulatory networks which control specific macrophage phenotypes is essential in identifying novel targets to correct macrophage mediated clinical disorders, often accompanied by inflammatory events. Since mesenchymal stromal cells (MSCs) have been shown to play key roles in regulating immune functio...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25282
更新日期:2014-11-01 00:00:00
abstract::A phenomenological model of biocide action against microbial biofilms was derived. Processes incorporated in the model include bulk flow in and out of a well-mixed reactor, transport of dissolved species into the biofilm, substrate consumption by bacterial metabolism, bacterial growth, advection of cell mass within th...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19960220)49:4<445::AID-BIT
更新日期:1996-02-20 00:00:00
abstract::Steady-state simulations using our previously developed structured kinetic model of antibody synthesis and secretion by hybridoma cells are used here in conjunction with factorial design analysis to identify intracellular parameters important in determining the specific antibody secretion rate and predict the dependen...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260390302
更新日期:1992-02-05 00:00:00
abstract::Phosphorus-31 nuclear magnetic resonance ((31)P NMR) was used to compare the anaerobic metabolism of glucose by suspended and gel-entrapped Saccharomyces bayanus cells. The fermentation of glucose was carried out in a reaction system with continuous circulation through the NMR sample tube. The intracellular pH and the...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260410607
更新日期:1993-03-15 00:00:00
abstract::Recently it has been established that low molecular weight displacers can be successfully employed for the purification of proteins in hydrophobic interaction chromatography (HIC) systems. This work investigates the utility of this technique for the purification of an industrial protein mixture. The study involved the...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10582
更新日期:2003-05-05 00:00:00
abstract::Catalytic membranes, obtained by immobilizing thermophilic beta-glycosidase onto nylon supports, were used in a nonisothermal bioreactor to study the effect of temperature gradients on the rate of enzyme reaction. Two experimental approaches were carried out to explain the molecular mechanisms by which the temperature...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:1998-07-05 00:00:00
abstract::An unadapted mixed methanogenic sludge transformed formaldehyde into methanol and formate. The methanol to formate ratio obtained was 1:1. Formaldehyde conversion proceeded without any lag phase, suggesting the constitutive character of the formaldehyde conversion enzymes involved. Because the rate of formaldehyde con...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10294
更新日期:2002-08-05 00:00:00
abstract::The design and operation of an industrial penicillin-V deacylation reactor is simulated, using a kinetic expression and mass transport parameters for the immobilized enzyme particles which were determined experimentally in a previous study. It is desirable to use a series of equalsized plug flow reactors with pH contr...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260261213
更新日期:1984-12-01 00:00:00
abstract::The efficiency of a versatile in vivo cascade involving a promiscuous alcohol dehydrogenase, obtained from a biodiversity search, and a Baeyer-Villiger monooxygenase was enhanced by the independent control of the production level of each enzyme to produce ε-caprolactone and 3,4-dihydrocoumarin. This goal was achieved ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27133
更新日期:2019-11-01 00:00:00
abstract::A novel multivariable control strategy is developed for alcohol (ethanol and n-pentanol) concentrations in the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(HB-co-HV), a biodegradable polymer by Paracoccus denitrificans ATCC 1774. This controller, which is developed to control the mole fraction of P(HB...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.1101
更新日期:2001-07-20 00:00:00
abstract::A structured kinetic model describing growth, differentiation, and penicillin production in submerged Penicillium chrysogenum fermentations is reported. The filamentous hyphae are divided into four distinct regions on the basis of the activities and structure of hyphal compartments, viz., actively growing (mainly apic...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19960905)51:5<558::AID-BIT
更新日期:1996-09-05 00:00:00
abstract::A novel technique for immobilization of Candida rugosa lipase onto anionic colloidal gas aphrons (CGAs) is described. CGAs are spherical microbubbles (10-100 microm) composed of an inner gas core surrounded by a surfactant shell. In this initial study, greater than 80% lipase (w/w) was effectively retained on the CGAs...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.1116
更新日期:2001-08-05 00:00:00
abstract::On-line NAD(P)H fluorescence and culture redox potential (CRP) measurements were utilized to investigate the role of Vitreoscilla hemoglobin (VHb) in perturbing oxygen metabolism of microaerobic Escherichia coli Batch cultures of a VHb-synthesizing E. coli strain and the iso-genic control under fully aerated condition...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260470309
更新日期:1995-08-05 00:00:00
abstract::After periodate oxidation of its glycosidic component, invertase was covalently bound onto three types of modified solid supports: glycidyl methacrylate, styrene-divinylbenzene copolymers, and bead cellulose. Direct reaction of the invertase aldehyde groups that were formed with amino groups of the support and use of ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260261011
更新日期:1984-10-01 00:00:00
abstract::Detachment from biofilms was evaluated using a mixed culture biofilm grown on primary wastewater in a tube reactor. The growth of biofilms and the detachment of biomass from biofilms are strongly influenced by hydrodynamic conditions. In a long-term study, three biofilms were cultivated in a biofilm tube reactor. The ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10503
更新日期:2003-03-05 00:00:00
abstract::It is a common practice in biotherapeutic manufacturing to define a fixed-volume feed strategy for nutrient feeds, based on historical cell demand. However, once the feed volumes are defined, they are inflexible to batch-to-batch variations in cell growth and physiology and can lead to inconsistent productivity and pr...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25684
更新日期:2015-12-01 00:00:00
abstract::Anaerobic biological wastewater treatment has numerous advantages over conventional aerobic processes; anaerobic biotechnologies, however, still have a reputation for low-quality effluents and operational instabilities. In this study, anaerobic bioreactors were augmented with an oxygen-transferring membrane to improve...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20219
更新日期:2005-02-20 00:00:00
abstract::We have studied the growth rate dependence of hepatitis B surface antigen (HBsAg) p24(s) monomer and lipoprotein particle synthesis produced in Saccharomyces cerevisiae using galactose-limited continuous culture. The hepatitis B virus S gene, which encodes the p24(s) monomer, is transcribed under the control of the GA...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19960305)49:5<578::AID-BIT
更新日期:1996-03-05 00:00:00
abstract::Microfiltration is an important unit operation in downstream processing. However, due to the influence of membrane fouling, prediction of the filtration performance for biological suspensions is difficult. This paper describes a modeling approach that allows a comprehensive description of filtration performance. On th...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19980720)59:2<189::aid-bit
更新日期:1998-07-20 00:00:00