Selective adsorption of plant products.

abstract：:Pronounced spatial nonuniformities in cell density, physiology, and activity frequently arise within densely packed immobilized cell supports. For a more fundamental understanding of immobilized cell phenomena, we have developed high-resolution microfluorimetric procedures to analyze local variations in both immobiliz...

journal_title：Biotechnology and bioengineering

authors： Kuhn RH,Peretti SW,Ollis DF

更新日期：1991-08-05 00:00:00

abstract：:A two-parameter deactivation model is proposed to describe the kinetics of activity stabilization for some enzymes. The single-step unimolecular mechanism exhibits non-first-order deactivation kinetics since the final enzyme state, E(1) is not completely inactivated. The usefulness of the model is demonstrated by appl...

journal_title：Biotechnology and bioengineering

authors： Sadana A,Henley JP

更新日期：1987-10-20 00:00:00

abstract：:Real-time polymerase chain reaction (PCR) is one of the most sensitive and accurate methods for quantifying transcript levels especially for those expressed at low abundance. The selective amplification of target DNA over multiple cycles allows its initial concentration to be determined. The amplification rate is a co...

journal_title：Biotechnology and bioengineering

authors： Mehra S,Hu WS

更新日期：2005-09-30 00:00:00

abstract：:The kinetic properties of collagen-bound firefly luciferase have been investigated. Under definite hydrodynamic conditions with low agitation in the reaction medium, the observed behavior is modified compared to the enzyme free in solution: reducing the stirring rate decreases the observed enzymatic activity. But diff...

journal_title：Biotechnology and bioengineering

authors： Blum LJ,Coulet PR

更新日期：1986-08-01 00:00:00

abstract：:Genetically transformed shooty teratomas of Atropa belladonna and a Duboisia leichhardtii x D. myoporoides hybrid were studied for biotransformation of tropane alkaloids in shake flasks and bioreactors. Although de novo synthesis of hyoscyamine and scopolamine was limited, shoots of both species were able to transloca...

journal_title：Biotechnology and bioengineering

authors： Subroto MA,Kwok KH,Hamill JD,Doran PM

更新日期：1996-03-05 00:00:00

abstract：:Galactose oxidase (E.C. 1.1.3.9) was covalently immobilized to chemically modified porous silica particles by reaction of the native enzyme with pendant benzoyl azide groups on the carrier. The enzyme loading on the carrier was 100-150 units per milliliter. The immobilized enzyme was incorporated into a hardware assem...

journal_title：Biotechnology and bioengineering

authors： Dahodwala SK,Weibel MK,Humphrey AE

更新日期：1976-12-01 00:00:00

abstract：:High throughput pretreatment (HTPH) and enzymatic hydrolysis systems are now vital for screening large numbers of biomass samples to investigate biomass recalcitrance over various pretreatment and enzymatic hydrolysis conditions. Although hydrothermal pretreatment is currently being employed in most high throughput ap...

journal_title：Biotechnology and bioengineering

authors： Li H,Gao X,Demartini JD,Kumar R,Wyman CE

更新日期：2013-11-01 00:00:00

abstract：:Membrane chromatography (MC) systems are finding increasing use in downstream processing trains for therapeutic proteins due to the unique mass-transfer characteristics they provide. As a result, there is increased need for model-based methods to scale-up MC units using data collected on a scaled-down unit. Here, a st...

journal_title：Biotechnology and bioengineering

authors： Ghosh P,Lin M,Vogel JH,Choy D,Haynes C,von Lieres E

更新日期：2014-08-01 00:00:00

abstract：:A recently discovered thermophilic bacterium, Geobacillus thermoglucosidasius M10EXG, ferments a range of C5 (e.g., xylose) and C6 sugars (e.g., glucose) and is tolerant to high ethanol concentrations (10%, v/v). We have investigated the central metabolism of this bacterium using both in vitro enzyme assays and 13C-ba...

journal_title：Biotechnology and bioengineering

authors： Tang YJ,Sapra R,Joyner D,Hazen TC,Myers S,Reichmuth D,Blanch H,Keasling JD

更新日期：2009-04-01 00:00:00

abstract：:The use of expanded beds of STREAMLINE ion exchange adsorbents for the direct extraction of an intracellular enzyme glucose-6-phosphate dehydrogenase (G6PDH) from unclarified yeast cell homogenates has been investigated. It has been demonstrated that such crude feedstocks can be applied to the bed without prior clarif...

journal_title：Biotechnology and bioengineering

authors： Chang YK,Chase HA

更新日期：1996-01-20 00:00:00

abstract：:We have developed a new cell surface display system using a major outer membrane protein of Pseudomonas aeruginosa OprF as an anchoring motif. Pseudomonas fluorescens SIK W1 lipase gene was fused to the truncated oprF gene by C-terminal deletion fusion strategy. The truncated OprF-lipase fusion protein was successfull...

journal_title：Biotechnology and bioengineering

authors： Lee SH,Choi JI,Han MJ,Choi JH,Lee SY

更新日期：2005-04-20 00:00:00

abstract：:We performed a macrokinetic and quantitative microbial investigation of a continuously operating bench-scale biofilter treating styrene-polluted gases. The device was filled with a mixture of peat and glass beads as packing medium and inoculated with the styrene-oxidizing strain, Rhodococcus rhodochrous AL NCIMB 13259...

journal_title：Biotechnology and bioengineering

authors： Zilli M,Converti A,Di Felice R

更新日期：2003-07-05 00:00:00

abstract：:The key objective for recombinant protein production in bacteria is the maximum exploitation of the cell factory's potential, whereby often strong expression vectors are used to increase product yield. If the metabolic load caused by recombinant expression exceeds the host's capacity, the system exhausts itself, resul...

journal_title：Biotechnology and bioengineering

authors： Grabherr R,Nilsson E,Striedner G,Bayer K

更新日期：2002-01-20 00:00:00

abstract：:Two instruments were developed to mechanize the handling of anaerobic microorganisms for microbial mutant isolation. The instruments automatically dispense liquid or agar medium to large and small 96-well platesand petridishes. Protocols were developed for inoculating different microorganisms, and calibration curves o...

journal_title：Biotechnology and bioengineering

authors： Stieglitz B,Defelice CP

更新日期：1986-09-01 00:00:00

abstract：:Saccharomyces cerevisiae is a widely used host organism for the production of heterologous proteins, often cultivated in glucose-based fed-batch processes. This production system however has many factors limiting the productivity, mainly towards the end of the fermentation. For the optimised production of a Camelid an...

journal_title：Biotechnology and bioengineering

authors： van de Laar T,Visser C,Holster M,López CG,Kreuning D,Sierkstra L,Lindner N,Verrips T

更新日期：2007-02-15 00:00:00

abstract：:Despite wide applications of l-tyrosine in the market, microbial overproduction of l-tyrosine has been a great challenge due to the complex gene regulations involved in its biosynthetic pathway. To this end, effects of knocking out tyrR on the l-tyrosine production were further explored during the strain development. ...

journal_title：Biotechnology and bioengineering

authors： Kim B,Binkley R,Kim HU,Lee SY

更新日期：2018-10-01 00:00:00

abstract：:We have previously reported the development of a novel protein expression system based on Ralstonia eutropha. In this study we report on the influence of gene copynumber on recombinant protein expression in R. eutropha. We compare recombinant gene stability and expression levels of chromosomal integration with a plasm...

journal_title：Biotechnology and bioengineering

authors： Srinivasan S,Barnard GC,Gerngross TU

更新日期：2003-10-05 00:00:00

abstract：:The nar promoter of Escherichia coli, which is maximally induced under anaerobic conditions in the presence of nitrate, was characterized to see whether the nar promoter cloned onto pBR322 can be used as an inducible promoter. To increase the expression level, the nar promoter was expressed in E. coli where active nit...

journal_title：Biotechnology and bioengineering

authors： Lee J,Cho MH,Lee J

更新日期：1996-12-05 00:00:00

abstract：:The stoichiometry and kinetics of the spontaneous, chemical reaction between pyrite and ferric iron was studied at 30, 45, and 70 degrees C in shake flasks at pH 1.5 by monitoring the ferrous iron, total iron, elemental sulfur, and sulfate concentration profiles in time. It was found that the sulfur moiety of pyrite w...

journal_title：Biotechnology and bioengineering

authors： Boogerd FC,van den Beemd C,Stoelwinder T,Bos P,Kuenen JG

更新日期：1991-06-20 00:00:00

abstract：:A pure culture of Pseudomonas fluorescens was used as a model system to study the kinetics of denitrification. An exponentially growing culture was harvested and resuspended in an anoxic acetate solution buffered with K/Na phosphate at pH values of 6.6, 7.0, 7.4, and 7.8. The temperature was kept at 28 degrees C in al...

journal_title：Biotechnology and bioengineering

authors： Almeida JS,Reis MA,Carrondo MJ

更新日期：1995-06-05 00:00:00

abstract：:Pyruvate production by Torulopsis glabrata was used as a model to study the mechanism of product inhibition and the strategy for enhancing pyruvate production. It was found that the concentration of cell growth and pyruvate deceased with the increase of NaCl and sorbitol concentrations. To enhance the osmotic stress r...

journal_title：Biotechnology and bioengineering

authors： Liu L,Xu Q,Li Y,Shi Z,Zhu Y,Du G,Chen J

更新日期：2007-07-01 00:00:00

abstract：:A nuclear magnetic resonance (NMR) method has been developed to monitor on-line lipase-catalyzed esterification reactions without the need to sample the reaction medium. The technique, through (1)H NMR, measures the concentrations of alcohol, ester, hydroxylic hydrogens in the organic phase, and hydroxylic hydrogens i...

journal_title：Biotechnology and bioengineering

authors： Sarazin C,Ergan F,Séguin JP,Goethals G,Legoy MD,Barbotin JN

更新日期：1996-09-20 00:00:00

abstract：:Synthetic mixtures of predominant lignocellulosic hexose sugars were supplemented with separate aliquots of three inhibitory compounds (furfural, hydroxymethylfurfural (HMF), and acetic acid) in a series of concentrations and fermented by the spent sulfite liquor (SSL)-adapted yeast strain Tembec T1 and the natural is...

journal_title：Biotechnology and bioengineering

authors： Keating JD,Panganiban C,Mansfield SD

更新日期：2006-04-20 00:00:00

abstract：:Kluyveromyces marxianus is a promising nonconventional yeast for biobased chemical production due to its rapid growth rate, high TCA cycle flux, and tolerance to low pH and high temperature. Unlike Saccharomyces cerevisiae, K. marxianus grows on low-cost substrates to cell densities that equal or surpass densities in ...

journal_title：Biotechnology and bioengineering

authors： McTaggart TL,Bever D,Bassett S,Da Silva NA

更新日期：2019-07-01 00:00:00

abstract：:Chinese hamster ovary (CHO) cells are the predominant host cell line for the production of biopharmaceuticals, a growing industry currently worth more than $188 billion USD in global sales. CHO cells undergo programmed cell death (apoptosis) following different stresses encountered in cell culture, such as substrate l...

journal_title：Biotechnology and bioengineering

authors： Henry MN,MacDonald MA,Orellana CA,Gray PP,Gillard M,Baker K,Nielsen LK,Marcellin E,Mahler S,Martínez VS

更新日期：2020-04-01 00:00:00

abstract：:The adsorption of cellulase on cellulose and a lignacious residue was examined by using cellulase from Trichoderma reesei, hardwood pretreated by dilute sulfuric acid under high pressure, and a lignacious residue prepared by a complete enzymatic hydrolysis of the pretreated wood. A significant amount of cellulase was ...

journal_title：Biotechnology and bioengineering

authors： Ooshima H,Burns DS,Converse AO

更新日期：1990-08-20 00:00:00

abstract：:It is a common practice in biotherapeutic manufacturing to define a fixed-volume feed strategy for nutrient feeds, based on historical cell demand. However, once the feed volumes are defined, they are inflexible to batch-to-batch variations in cell growth and physiology and can lead to inconsistent productivity and pr...

journal_title：Biotechnology and bioengineering

authors： Zhang A,Tsang VL,Moore B,Shen V,Huang YM,Kshirsagar R,Ryll T

更新日期：2015-12-01 00:00:00

abstract：:Experimental and theoretical studies are conducted to understand the dynamic behavior of a continuous-flow fixed-bed reactor in which an esterification is catalyzed by an immobilized enzyme in an organic solvent medium. The experimental system consists of a commercial immobilized lipase preparation known as Lipozyme a...

journal_title：Biotechnology and bioengineering

authors： Mensah P,Gainer JL,Carta G

更新日期：1998-11-20 00:00:00

abstract：:Genetically engineered microbes have been intensively investigated as a means for the cost-effective production of isoprenoids. Bacillus subtilis is a promising microbial host for this purpose because of its fast growth rate and GRAS (generally regarded as safe) status. To date, development of this host has been impai...

journal_title：Biotechnology and bioengineering

authors： Zhou K,Zou R,Zhang C,Stephanopoulos G,Too HP

更新日期：2013-09-01 00:00:00

abstract：:Foam separation may have potential for protein recovery. However, for foam separation to be a viable protein recovery technique it is important to demonstrate, not only that high enrichments and recoveries can be achieved for single proteins, but also that high enrichments and recoveries, together with selectivity of ...

journal_title：Biotechnology and bioengineering

authors： Brown AK,Kaul A,Varley J

更新日期：1999-02-05 00:00:00